Laboratory Procedure
pyrosequencing
Definitions related to pyrosequencing:
  • A DNA sequencing method that uses a "sequencing by synthesis" strategy. The strand complementary to a template is synthesized a single base at a time through the sequential addition of individual solutions of each base. Incorporation of a base into the growing strand generates free pyrophosphate that is detected as light, as generated by a luciferase-catalyzed reaction.
    NCI
    U.S. National Cancer Institute, 2021
  • A DNA sequencing technique that is based on the detection of released pyrophosphate during DNA synthesis.
    CDISC Terminology
    Clinical Data Interchange Standards Consortium (CDISC), 2021
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