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Current Microbiology Jan 2021The formal scientific name "Acinetobacter mesopotamicus" has recently been proposed for bacterial strain GC2 isolated from soil (Curr Microbiol 77:3192-3200). However,...
Strain "Acinetobacter mesopotamicus" GC2 Does Not Represent a Novel Species, but Belongs to the Species Acinetobacter lwoffii as Revealed by Whole-Genome Sequence-Based Analysis.
The formal scientific name "Acinetobacter mesopotamicus" has recently been proposed for bacterial strain GC2 isolated from soil (Curr Microbiol 77:3192-3200). However, the recalculation of the average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) parameters revealed that their values for the genome sequences of GC2 and Acinetobacter lwoffii NCTC 5866 were as high as 95.7% and 66.1%, respectively. Moreover, the ANI and dDDH values for the genome of GC2 tested against those of 10 reference strains of A. lwoffii were 95.5-96.7% and 65.5-74.2%, respectively, and fall within the intraspecies range of these values known for A. lwoffii. These data indicate that strain GC2 belongs to A. lwoffii and contradicts the results of the authors of the proposal, who found the ANI and dDDH values for the GC2 and A. lwoffii DSM 2403 genomes to be 88.87% and 36.1%, respectively. The misclassification of strain GC2 is likely to result from the use of an incorrect reference genome sequence for in silico comparative analyses.
Topics: Acinetobacter; Bacterial Typing Techniques; Base Composition; DNA, Bacterial; Fatty Acids; Nucleic Acid Hybridization; Phylogeny; RNA, Ribosomal, 16S; Sequence Analysis, DNA
PubMed: 33136203
DOI: 10.1007/s00284-020-02275-x -
Systematic and Applied Microbiology Mar 2019In 1986, Bouvet and Grimont delineated two related taxa of the genus Acinetobacter termed genospecies (GS) 8 and 9. They proposed the name Acinetobacter lwoffii for GS8,...
In 1986, Bouvet and Grimont delineated two related taxa of the genus Acinetobacter termed genospecies (GS) 8 and 9. They proposed the name Acinetobacter lwoffii for GS8, which included the supposed type strain (CIP 64.10). As the authenticity of CIP 64.10 was later questioned, this study aimed at reassessing the taxonomy of these genospecies. We investigated 52 strains of GS8 or GS9, including CIP 64.10 and the genuine type strain of A. lwoffii (NCTC 5866). All strains were subjected to the genus-wide comparative analyses of MALDI-TOF whole-cell mass spectra, rpoB gene sequences and metabolic traits while whole-genome sequences were analysed for 16 strains. The strains were classified into two distinct groups corresponding to GS8 (n=15) and GS9 (n=37). CIP 64.10 fell within GS8 whereas NCTC 5866 belonged to GS9. Intraspecies ANIb values for the genomes of GS8 (n=6) and GS9 (n=10) were ≥96.1% and ≥95.4%, respectively, whereas the ANIb values between them were 86.8-88.6%. Based on core genome phylogeny, GS8 and GS9 formed a distinct clade within the genus, with two respective, strongly supported subclades. GS8 and GS9 were similar in physiological and catabolic properties but were separable by MALDI-TOF MS. We conclude that the name A. lwoffii pertains to GS9 and not to GS8 as originally assumed and that these groups represent two species. We propose the name Acinetobacter pseudolwoffii sp. nov. for GS8, with ANC 5044 (=CCM 8638=CCUG 67963=CIP 111642) as the type strain, and provide the emended description of A. lwoffii.
Topics: Acinetobacter; Bacterial Typing Techniques; DNA-Directed RNA Polymerases; Genes, Bacterial; Phylogeny; Whole Genome Sequencing
PubMed: 30392743
DOI: 10.1016/j.syapm.2018.10.004 -
Biomedical Papers of the Medical... Jun 2019Various food-producing animals have been recognized in recent years as a potential reservoir for the spread of antibiotic resistant bacteria that may pose a risk to...
BACKGROUND
Various food-producing animals have been recognized in recent years as a potential reservoir for the spread of antibiotic resistant bacteria that may pose a risk to human health and therefore their dissemination in the food production chain needs to be assessed.
METHODS
In this study, 450 boot swabs from chicken farms were analyzed for the presence of antimicrobial resistance with a focus on β-lactams resistance in Acinetobacter species.
RESULTS
Two β-lactamase-encoding genes were first time identified in Acinetobacter lwoffii and Acinetobacter schindleri isolates. The deduced amino acid sequence of OXA-496 shared 93.8% identity with OXA-363. The second OXA-134-like enzyme, OXA-537, had the highest sequence identity (97.8%) with OXA-235 and OXA-237.
CONCLUSIONS
The results of this study illustrate the occurrence of new OXA-134-like β-lactamases, called OXA-496 and OXA-537, carrying strains of Acinetobacter lwoffii and Acinetobacter schindleri in chicken farm litter, and highlight the possible role of Acinetobacter as a reservoir of resistance genes.
Topics: Acinetobacter; Animals; Chickens; Czech Republic; Drug Resistance, Bacterial; Farms; Microbial Sensitivity Tests; beta-Lactam Resistance; beta-Lactamases
PubMed: 30150791
DOI: 10.5507/bp.2018.037 -
Allergy Jun 2023The microbiome associations of food protein-induced enterocolitis syndrome (FPIES) are understudied. We sought to prospectively define the clinical features of FPIES in...
BACKGROUND
The microbiome associations of food protein-induced enterocolitis syndrome (FPIES) are understudied. We sought to prospectively define the clinical features of FPIES in a birth cohort, and investigate for the evidence of gut dysbiosis.
METHODS
We identified children diagnosed with FPIES in the Gastrointestinal Microbiome and Allergic Proctocolitis Study, a healthy infant cohort. Children were assessed and stools were collected at each well child visit. The clinical features of the children with FPIES were summarized. Stool microbiome was analyzed using 16S rRNA sequencing comparing children with and without FPIES.
RESULTS
Of the 874 children followed up for 3 years, 8 FPIES cases (4 male) were identified, yielding a cumulative incidence of 0.92%. The most common triggers were oat and rice (n = 3, each) followed by milk (n = 2). The children with FPIES were more likely to have family history of food allergy (50% vs. 15.9% among unaffected, p = .03). The average age of disease presentation was 6 months old. During the first 6 months of life, stool from children with FPIES contained significantly less Bifidobacterium adolescentis, but more pathobionts, including Bacteroides spp. (especially Bacteroides fragilis), Holdemania spp., Lachnobacterium spp., and Acinetobacter lwoffii. The short-chain fatty acid (SCFA)-producing Bifidobacterium shunt was expressed significantly less in the stool from FPIES children.
CONCLUSIONS
In this cohort, the cumulative incidence over the 3-year study period was 0.92%. During the first 6 months of life, children with FPIES had evidence of dysbiosis and SCFA production pathway was expressed less in their stool, which may play an important role in the pathogenesis of FPIES.
Topics: Child; Humans; Infant; Male; Prospective Studies; Dysbiosis; RNA, Ribosomal, 16S; Dietary Proteins; Syndrome; Food Hypersensitivity; Enterocolitis; Allergens
PubMed: 36635218
DOI: 10.1111/all.15644 -
Infectious Disorders Drug Targets 2015Acinetobacter species are ubiquitous in the environment and are important causative agent for nososcomial infection especially in immunocompromised patients. Multi drug...
BACKGROUND
Acinetobacter species are ubiquitous in the environment and are important causative agent for nososcomial infection especially in immunocompromised patients. Multi drug resistant Acinetobacter lwoffii are emerging as a pathogen in neoanatal sepsis.
AIMS AND OBJECTIVE
This study was aimed to evaluate the clinical and antibiotic profile of Acinetobacter lwoffii.
MATERIAL AND METHODS
This study was done on blood samples from neonates admitted to neonatal intensive care unit during a period of one year from January to December 2012, who developed Acinetobacter infection. The diagnosis of isolates and antibiotic susceptibility testing was done by both conventional as well as by automated system.
RESULTS
Out of total 13,133 blood samples received for culture, 1418(10.8%) were from NICU. Ninety (6.3%) isolates were found to be positive for the growth of Acinetobacter species. Of these isolates 31.11% were found to be Acinetobacter lwoffii, 68.9% were Acinetobacter baumannii calcaetius complex. Acinetobacter lwoffii isolates were most commonly sensitive to imepenem 16(57%), cotrimoxazole 9(32%), ciprofloxacin 6(21%) followed by amoxyclavulanic acid 2(7%) and cefuroxime 1(3.5%).
CONCLUSION
Multi drug resistant Acinetobacter lwoffii infection is increasing particularly in premature and very low-birth weight neonates. Judicious and timely antibiotic use in NICUs are one of the important key in controlling multi-drug resistant Acinetobacter infection and improving clinical outcome.
Topics: Acinetobacter; Acinetobacter Infections; Acinetobacter baumannii; Anti-Bacterial Agents; Blood; Child; Communicable Diseases, Emerging; Cross Infection; Drug Resistance, Multiple, Bacterial; Humans; India; Infant, Newborn; Intensive Care Units, Neonatal; Male; Microbial Sensitivity Tests; Sepsis
PubMed: 26307173
DOI: 10.2174/1871526515666150826114745 -
Transboundary and Emerging Diseases Dec 2018Acinetobacter lwoffii, a serious human pathogen, has been identified as a cause of nosocomial infections such as bacteremia, pneumonia and meningitis. There are only a...
Acinetobacter lwoffii, a serious human pathogen, has been identified as a cause of nosocomial infections such as bacteremia, pneumonia and meningitis. There are only a few studies reporting A. lwoffii as a pathogen of fish. During 2016 and 2017, six bacterial strains, isolated from diseased fish of the Schizothorax genus, were identified as A. lwoffii by morphology, biochemical tests, 16S rDNA and gyrB gene sequencing analysis. One of these isolates was selected for experimental infection of Sclizothorax prenanti, Schizothorax davidi and Schizothorax wangchiachii, to confirm its pathogenicity. Experimentally infected fish showed similar symptoms to those observed in fish after natural outbreaks. Susceptibility of the isolates to 14 antibiotics was tested using a disc diffusion method; all isolates were resistant to cephalothin, aminoglycosides and β-lactams, and sensitive only to some fluoroquinolones and tetracyclines. Histological examination revealed that A. lwoffii infection could cause pathological lesions in multiple organs and tissues, especially in liver, kidney, spleen and heart. These lesions included extensive haemorrhage, vacuolar degeneration, necrosis and inflammatory cell infiltration. To our knowledge, this is the first report on A. lwoffii as a virulent pathogen for fish of the Schizothorax genus.
Topics: Acinetobacter; Acinetobacter Infections; Animals; Anti-Bacterial Agents; China; Communicable Diseases, Emerging; DNA Gyrase; Drug Resistance, Multiple, Bacterial; Fish Diseases; Fishes; RNA, Ribosomal, 16S; Sequence Analysis
PubMed: 30239149
DOI: 10.1111/tbed.12957 -
International Microbiology : the... Nov 2023Ingesting marine plastics is increasingly common in cetaceans, but little is known about their potential effects. Here, by utilizing 16S rRNA gene sequencing, we...
Ingesting marine plastics is increasingly common in cetaceans, but little is known about their potential effects. Here, by utilizing 16S rRNA gene sequencing, we profiled the intestinal bacterial communities of a stranded Risso's dolphin (Grampus griseus) which died because of the ingestion of rubber gloves. In this study, we explored the potential relationships between starvation raised by plastic ingestion with the dolphin gut microbiota. Our results showed significant differences in bacterial diversity and composition among the different anatomical areas along the intestinal tract, which may be related to the intestinal emptying process under starvation. In addition, the intestinal bacterial composition of the Risso's dolphin showed both similarity and divergence to that of other toothed whales, suggesting potential roles of both host phylogeny and habitat shaping of the cetacean intestinal microbiome. Perhaps, the microbiota is reflecting a potentially disordered intestinal microbial profile caused by the ingestion of macro-plastics which led to starvation. Moreover, two operational taxonomic units (0.17% of the total reads) affiliated with Actinobacillus and Acinetobacter lwoffii were detected along the intestinal tract. These bacterial species may cause infections in immunocompromised dolphins which are malnourished. This preliminary study profiles the intestinal microbiota of a Risso's dolphin, and provides an additional understanding of the potential relationships between starvation raised by ingesting macro-plastics with cetacean gut microbiota.
Topics: Animals; Dolphins; Gastrointestinal Microbiome; RNA, Ribosomal, 16S; Eating
PubMed: 37059916
DOI: 10.1007/s10123-023-00355-z -
Biology Sep 2021Microbial life can be supported at subzero temperatures in permafrost up to several million years old. Genome analysis of strains isolated from permafrost provides a...
Genome Analysis of Strains Isolated from Permafrost Soils Aged from 15 Thousand to 1.8 Million Years Revealed Their Close Relationships with Present-Day Environmental and Clinical Isolates.
Microbial life can be supported at subzero temperatures in permafrost up to several million years old. Genome analysis of strains isolated from permafrost provides a unique opportunity to study microorganisms that have not previously come into contact with the human population. is a typical soil bacterium that has been increasingly reported as hospital pathogens associated with bacteremia. In order to identify the specific genetic characteristics of ancient permafrost-conserved strains of and their differences from present-day clinical isolates, we carried out a genome-wide analysis of five strains of isolated from permafrost aged from 15 thousand to 1.8 million years. Surprisingly, we did not identify chromosomal genetic determinants that distinguish permafrost strains from clinical isolates and strains from other natural habitats. Phylogenetic analysis based on whole genome sequences showed that permafrost strains do not form a separate cluster and some of them are most closely related to clinical isolates. The genomes of clinical and permafrost strains contain similar mobile elements and prophages, which indicates an intense horizontal transfer of genetic material. Comparison of plasmids of modern and permafrost strains showed that plasmids from the modern strains are enriched with antibiotic resistance genes, while the content of genes for resistance to heavy metals and arsenic is nearly the same. The thawing of permafrost caused by global warming could release new potentially pathogenic strains of .
PubMed: 34571748
DOI: 10.3390/biology10090871 -
Bioresource Technology Jan 2023A novel biobased composite was developed for the removal of phosphate (P) and atrazine from agricultural wastewater. A composite with strong P affinity and good...
A novel biobased composite was developed for the removal of phosphate (P) and atrazine from agricultural wastewater. A composite with strong P affinity and good biocompatibility, synthesized from La and polydopamine (PDA), was immobilized onto an atrazine-degrading bacterium Acinetobacter lwoffii DNS32 (La/PDA/DNS32). Following Box-Behnken design optimization, the maximum removal rate of P (500 mg L) and atrazine (100 mg L) by La/PDA/DNS32 reached 28 % and 100 %, respectively. Density functional theory calculations revealed that La/PDA had more negative adsorption energy (-5.90 eV) than PDA alone and exhibited prominent electrophilic sites. Additionally, La/PDA-induced sorption of atrazine improved transmembrane transport and enhanced expression of degradation-associated genes in strain DNS32. La/PDA nanoparticles surrounding strain DNS32 provided a shielding effect and exhibited desirable biostability, thermal stability, and acid-alkaline resistance under contamination stress. This study demonstrates the promising potential of La/PDA/DNS32 in reducing the P and atrazine pollution caused by agricultural production.
Topics: Atrazine; Lanthanum; Adsorption; Phosphates
PubMed: 36351531
DOI: 10.1016/j.biortech.2022.128266 -
Fukushima Journal of Medical Science Nov 2023The incidence of Acinetobacter infections has increased in recent years. Acinetobacter infections are resistant to most antibiotics and can be found in hospitalized...
The incidence of Acinetobacter infections has increased in recent years. Acinetobacter infections are resistant to most antibiotics and can be found in hospitalized patients. Pregnancies complicated by severe sepsis or septic shock are associated with a higher rate of preterm labor and delivery, fetal infection, and operative delivery. This case report describes septic shock due to Acinetobacter lwoffii infection in the 31st week of gestation. A 47-year-old woman, with a gestation of 31 weeks and one day, presented with a fever, and signs of bacterial infection on laboratory tests. Although the patient was started on tazobactam/piperacillin, she went into septic shock, and was transferred to our hospital. Cesarean section was performed at a gestation of 31 weeks and 4 days because of severe maternal pneumonia and non-reassuring fetal status. A. lwoffii was detected in blood cultures collected at the previous hospital, and susceptibility to piperacillin and meropenem to A. lwoffii was confirmed. The pneumonia responded to antibiotic treatment and there were no findings of infection in the neonate. Maternal sepsis is an infrequent but important complication, causing significant maternal and fetal morbidity and fetal and neonatal mortality; therefore, early antibiotic therapy is required to improve the clinical outcome.
Topics: Infant, Newborn; Humans; Pregnancy; Female; Middle Aged; Shock, Septic; Acinetobacter Infections; Cesarean Section; Anti-Bacterial Agents; Piperacillin; Pneumonia
PubMed: 37766560
DOI: 10.5387/fms.2022-43