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Journal of Microbiology, Immunology,... Dec 2019Acinetobacter is an aerobic, gram-negative coccobacillus, which causes nosocomial infections including bacteremia. Recent development of molecular techniques has made... (Comparative Study)
Comparative Study
The clinical characteristics of Acinetobacter bacteremia differ among genomospecies: A hospital-based retrospective comparative analysis of genotypically identified strains.
BACKGROUND/PURPOSE
Acinetobacter is an aerobic, gram-negative coccobacillus, which causes nosocomial infections including bacteremia. Recent development of molecular techniques has made classification of the Acinetobacter genomospecies possible, but there are still only a few studies comparing clinical features of the subspecies. We investigated bacteremia caused by Acinetobacter, isolated subspecies, and compared clinical features for each group.
METHODS
A retrospective analysis of Acinetobacter bacteremia cases was made in a 900-bed hospital in Japan. In addition to conventional procedures, subspecies identification based on rpoB sequence was made, and comparison of clinical characteristics between each subspecies were analyzed.
RESULTS
We collected 35 cases (Acinetobacter baumannii 14, A. nosocomialis 12, Acinetobacter ursingii 6, and A. seifertii 3). All of the A. seifertii bacteremia cases were blood stream infection occurring in cerebrovascular disease patients, showing particularly higher incidence of shock (100%) and high Pitt bacteremia score (PBS) (6.33 ± 2.52) in comparison to A. baumannii (43% and 2.86 ± 2.25, respectively). Sequential Organ Failure Assessment (SOFA) score and the PBS were slightly higher in A. nosocomialis in comparison to A. baumannii, and the 7 day mortality rate was higher in A. nosocomialis (25%) than in A. baumannii (7%), though this difference was not found to be significant.
CONCLUSIONS
A.seifertii, the recently defined novel species, showed distinctive clinical features of bacteremia. And, in contrast to previous studies, the severity of A. nosocomialis infection was not lower than that of A. baumannii, which might suggest the influence of local epidemiology. Further characterization of these subspecies should be continued.
Topics: Acinetobacter; Acinetobacter Infections; Acinetobacter baumannii; Adult; Aged; Aged, 80 and over; Anti-Bacterial Agents; Bacteremia; Bacterial Proteins; Cross Infection; Female; Genotype; Hospitals; Humans; Japan; Male; Microbial Sensitivity Tests; Middle Aged; Retrospective Studies; Young Adult
PubMed: 31813733
DOI: 10.1016/j.jmii.2019.09.007 -
Clinical Pediatrics Nov 2022Acute suppurative parotitis (ASP) of neonates is a rare condition characterized by irritability, erythema, and tenderness of the affected gland. (Review)
Review
BACKGROUND
Acute suppurative parotitis (ASP) of neonates is a rare condition characterized by irritability, erythema, and tenderness of the affected gland.
METHODS/RESULTS
Only few cases have been reported in Engilsh literature, mostly in male neonates, in a unilateral fashion. In our case, a polymicrobial etiology (, and ) was found. Based on the review of the microbiological findings of cases of ASP in English literature for the years 1970 to 2020, is the most commonly isolated microorganism (47% of the total 65 patients). Our patient was born with a C-section procedure and was not breast-fed, making dysbiosis along with the usage of the feeding bottle, possible risk factors for the development of ASP.
CONCLUSIONS
ASP may be due to polymicrobial etiology. Initial presentation in neonates may not include typical signs and symptoms, like fever. Aseptic technique of oral procedures is of utmost importance also in immune-competent neonates.
Topics: Anti-Bacterial Agents; Female; Humans; Infant; Infant, Newborn; Male; Parotitis; Staphylococcal Infections; Staphylococcus aureus; Suppuration
PubMed: 35678072
DOI: 10.1177/00099228221102712 -
New Microbes and New Infections Mar 2018species can be important opportunistic pathogens in humans, especially in healthcare settings. We report here the first isolation of from an animal species; it was...
species can be important opportunistic pathogens in humans, especially in healthcare settings. We report here the first isolation of from an animal species; it was isolated from a canine urinary tract infection, and phenotypic identification proved unreliable.
PubMed: 29556400
DOI: 10.1016/j.nmni.2017.11.007 -
Microbial Pathogenesis Feb 2019The study aims to explore the characteristic microorganisms of the common tongue coatings in patients with gastric cancer (GC).
PURPOSE
The study aims to explore the characteristic microorganisms of the common tongue coatings in patients with gastric cancer (GC).
METHODS
A total of 115 GC patients were assigned to four groups: White-thin coating (W-thin) group, White-thick coating (W-thick) group, Yellow-thin coating (Y-thin) group and Yellow-thick coating (Y-thick) group. Thirty-five healthy volunteers with White-thin coating were recruit as controls. High-throughput sequencing was used to describe the microbial community of the tongue coatings based on 16S rRNA and 18S rRNA genes. Multi-factors statistical analysis was carried out to present the microbial biomarkers of the tongue coating in GC patients.
RESULTS
At bacterial phylum level, Saccharibacteria had higher relative abundance in W-thick group than W-thin group, Proteobacteria was more abundant in W-thin group than Y-thick group and less abundant in Y-thick group than Y-thin group. At fungal genus level, Guehomyces and Aspergillus presented to be significantly different among the common tongue coatings. Forteen significantly increased taxa were sorted out as the microbial biomarkers of common tongue coatings by LEfSe and ROC analysis. At species level, bacterial Capnocytophaga leadbetteri and fungal Ampelomyces_sp_IRAN_1 may be the potential biomarkers of W-thin coating, four bacterial species (Megasphaera micronuciformis, Selenomonas sputigena ATCC 35185, Acinetobacter ursingii, Prevotella maculosa) may be the potential biomarkers of W-thick coating. In general, the white coatings held more complex commensal relationship than the yellow coatings.
CONCLUSION
The common tongue coating owned characteristic microorganisms and special commensal relationship in the GC patients.
Topics: Aged; Bacteria; Cluster Analysis; DNA, Bacterial; DNA, Fungal; DNA, Ribosomal; Female; Fungi; Humans; Male; Microbiota; Middle Aged; Phylogeny; RNA, Ribosomal, 16S; RNA, Ribosomal, 18S; ROC Curve; Sequence Analysis, DNA; Stomach Neoplasms; Tongue
PubMed: 30508628
DOI: 10.1016/j.micpath.2018.11.051 -
Zhonghua Er Bi Yan Hou Tou Jing Wai Ke... Sep 2023To investigate the characteristics of nasal flora and the pathogenic role of differential microbiome in patients with allergic rhinitis (AR) and non-allergic rhinitis...
To investigate the characteristics of nasal flora and the pathogenic role of differential microbiome in patients with allergic rhinitis (AR) and non-allergic rhinitis (nAR). Thirty-five patients with AR who attended the rhinology outpatient clinic of the Second Hospital of Harbin Medical University from February to July 2022 were selected. A total of 35 nAR patients were selected as the test group, and 20 cases of healthy people with physical examination at the same period were selected as the control group, including 39 males and 51 females, aged 8 to 55 years. 16SrDNA High-throughput sequencing was used to analyze the relative abundance from nasal flora in the three groups of subjects. Alpha diversity index analysis was conducted with R software, and differences between groups were analyzed with LEfSe, Metastats, and tests. At the same time, the role of microbiome and its relationship with environmental factors were analyzed with R software. There was a significant difference in the bacterial composition of the samples from the three groups, with the relative abundance of Staphylococcus aureus (=0.032) and Corynebacterium proinquum (=0.032) within the AR group being significantly higher than that of the nAR group, and that of Lactobacillus murinus, Lactobacillus kunkeei, and Alcaligenes faecalis ( value was 0.016, 0.005, and 0.001, respectively) being significantly lower than that of the nAR group. The relative abundance of Ackermannia muciniphila within the nAR group was higher than that of the control group (=0.009). Correlation analysis of environmental factors showed a negative correlation between Lactobacillus kunkeei and IgE (=0.044), and a positive correlation between Lactobacillus murinus and age (=0.019). AR and nAR random forest prediction models were constructed for the five genera, respectively, and the area under the curve (AUC) of the models of Streptococcus-SP-FF10, Pseudoalteromonas luteoviolacea, Pseudomonas parafulva, Acinetobacter ursingii, and Azotobacter chroococcum in the AR group was 100% (95%CI: 100% to 100%). The AUC for the Pseudomonas parafulva, Azotobacter chroococcum, Closoridium baratii, Turicibacter-SP-H121, and Streptococcus lutetiensis models in the nAR group was 98.4% (95%CI: 94.9% to 100%). The distribution of nasal flora in AR patients, nAR patients and healthy subjects is significantly different, and the changes of bacterial flora abundance are significantly related to the occurrence of AR and nAR. Combined detection of microbiota has the potential to diagnose AR and nAR patients.
Topics: Female; Male; Humans; Rhinitis; Rhinitis, Allergic
PubMed: 37675527
DOI: 10.3760/cma.j.cn115330-20221012-00605 -
Microbial Ecology Feb 2019Despite the considerable role of aquatic plant-associated bacteria in host plant growth and nutrient cycling in aquatic environments, the mode of their plant...
Despite the considerable role of aquatic plant-associated bacteria in host plant growth and nutrient cycling in aquatic environments, the mode of their plant colonization has hardly been understood. This study examined the colonization and competition dynamics of a plant growth-promoting bacterium (PGPB) and two plant growth-inhibiting bacteria (PGIB) in the aquatic plant Lemna minor (common duckweed). When inoculated separately to L. minor, each bacterial strain quickly colonized at approximately 10 cells per milligram (plant fresh weight) and kept similar populations throughout the 7-day cultivation time. The results of two-membered co-inoculation assays revealed that the PGPB strain Aquitalea magnusonii H3 consistently competitively excluded the PGIB strain Acinetobacter ursingii M3, and strain H3 co-existed at almost 1:1 proportion with another PGIB strain, Asticcacaulis excentricus M6, regardless of the inoculation ratios (99:1-1:99) and inoculation order. We also found that A. magnusonii H3 exerted its growth-promoting effect over the negative effects of the two PGIB strains even when only a small amount was inoculated, probably due to its excellent competitive colonization ability. These experimental results demonstrate that there is a constant ecological equilibrium state involved in the bacterial colonization of aquatic plants.
Topics: Agricultural Inoculants; Araceae; Bacteria; Biodiversity; Plant Roots
PubMed: 30603770
DOI: 10.1007/s00248-018-1306-x -
Seminars in Ophthalmology May 2024To study the metagenomics of the microbes isolated from the lacrimal sac in patients with failed dacryocystorhinostomy (DCR).
PURPOSE
To study the metagenomics of the microbes isolated from the lacrimal sac in patients with failed dacryocystorhinostomy (DCR).
METHODS
A prospective study was performed on 10 consecutive patients with failed DCR. Lacrimal sac samples were obtained for metagenomic analysis during the revision endoscopic DCR. The samples were collected intraoperatively after a full-length lacrimal sac marsupialization and immediately transported on ice to the laboratory. A whole shotgun metagenome sequencing was performed on the Illumina platform following DNA extraction and library preparation. The downstream analysis of the samples was performed using various software packaged in the Squeeze Metapipeline v1.3.0 and marker gene-based metagenomic phylogenetic analysis using MetaPhlAn4.
RESULTS
The five major phyla identified across the samples of failed DCR include Proteobacteria, Firmicutes, Actinobacteria, Bacteroidetes, and Fusobacteria (Figure 1). The prevalent species include Stenotrophomonas maltophilia, Pseudomonas juntendi, Streptococcus pneumoniae, Acinetobacter ursingii, Citrobacter koseri, and Cutibacterium acnes (Figure 2). Among the other organisms identified, few were from genera candida and mezorhizobium. Among the viruses, the most abundant was the BeAn 58058 virus. It was interesting to note the occasional presence of plasmodium and toxoplasma species. The functional category distribution of KEGG (Kyoto encyclopedia of genes and genomes) data showed microbial metabolism to be the most involved function, followed by cellular processes.
CONCLUSION
This is the first whole metagenome sequencing of the lacrimal sac contents from failed DCR patients. The organisms identified varied significantly from those isolated from patients with primary acquired nasolacrimal duct obstruction (PANDO) using similar techniques and reflect altered lacrimal microbiota in surgically unsuccessful DCRs.
Topics: Humans; Dacryocystorhinostomy; Nasolacrimal Duct; Lacrimal Duct Obstruction; Prospective Studies; Phylogeny; Microbiota; Treatment Outcome
PubMed: 38461372
DOI: 10.1080/08820538.2024.2327481 -
Deutsche Medizinische Wochenschrift... Aug 2022A 19-year-old patient presented as an inpatient with an acute flare-up of ulcerative colitis for about six weeks and symptom progression for one and a half weeks. The...
HISTORY AND CLINICAL FINDINGS
A 19-year-old patient presented as an inpatient with an acute flare-up of ulcerative colitis for about six weeks and symptom progression for one and a half weeks. The patient was treated with topical and oral 5-aminosalicylic acid (5-ASA) preparations and oral prednisolone. With an intensification of the prednisolone dose, administration of a monoclonal antibody (Vedolizumab) and antibiotic therapy, inpatient discharge against medical advice. Oral administration of ciprofloxacin due to continued elevated infection parameters. The 5-ASA administration was continued. In the following two weeks, recurrent episodes of fever and renewed inpatient admission.
INVESTIGATIONS AND DIAGNOSIS
Microbiological detection of in blood cultures. Echocardiographic evidence of pericardial effusion. Transesophageal echocardiography revealed no evidence of infective endocarditis. Serological evidence of elevated cardiac enzyme levels. An MRI scan of the heart confirmed the diagnosis of perimyocarditis.
TREATMENT AND COURSE
Intensive medical care and resistogram-based antibiotic therapy with meropenem, stopping the topical and oral 5-ASA doses and not continuing the integrin antagonist therapy. In addition, start of guideline-based cardiac insufficiency therapy and change of therapy to a tumor necrosis factor alpha blocker. Under the accelerated application scheme, stabilization and improvement of the general condition protracted. After two months of hospitalization, the patient was discharged to outpatient care.
DISCUSSION
Perimyocarditis may occur after long-term administration of 5-ASA. The integrin antagonist as a new therapy can also be a causal factor. Therapeutic management when the cause of the complaint is unclear depends on the symptoms and the most likely cause of the disease. If there is no response to therapy, carry out early re-evaluations.
Topics: Adult; Anti-Bacterial Agents; Anti-Inflammatory Agents, Non-Steroidal; Colitis, Ulcerative; Humans; Immunosuppressive Agents; Integrins; Mesalamine; Pharmaceutical Preparations; Prednisolone; Young Adult
PubMed: 35970187
DOI: 10.1055/a-1894-4615 -
Vector Borne and Zoonotic Diseases... Nov 2020We sought to evidence the presence of emerging bacterial pathogens in clothes lice collected from sheltered homeless individuals from Marseille, France. During the...
We sought to evidence the presence of emerging bacterial pathogens in clothes lice collected from sheltered homeless individuals from Marseille, France. During the 2013-2018 period, a total of 507 lice were collected from 37 individuals and were processed for molecular analysis. We reported a low prevalence of DNA carriage (1.2%). No louse tested positive for sp., , sp., sp., , or . A comparison with studies conducted before 2013 showed a 17.5-fold reduction in the rate of DNA positivity. By contrast, a high prevalence of species DNA carriage (40.8%), mostly (32.9%), was observed, tending to increase over time. In addition, we detected DNA in clothes lice for the first time. Genotypic characterization and antimicrobial susceptibility testing of isolates from clothes lice are needed to assess whether these strains present in lice are similar to those responsible for human infections and harbor mechanisms of resistance against antibiotics.
Topics: Acinetobacter baumannii; Animals; Bartonella quintana; Clothing; France; Ill-Housed Persons; Host-Pathogen Interactions; Phthiraptera; Phylogeny; Prevalence
PubMed: 32366193
DOI: 10.1089/vbz.2019.2603 -
Emerging Infectious Diseases Jan 2016We report a community-acquired bloodstream infection with Acinteobacter ursingii in an HIV-negative woman who injected drugs. The infection was successfully treated with...
We report a community-acquired bloodstream infection with Acinteobacter ursingii in an HIV-negative woman who injected drugs. The infection was successfully treated with meropenem. Species identification was performed by using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Improved identification of Acinetobacter spp. by using this method will help identify clinical effects of this underdiagnosed pathogen.
Topics: Acinetobacter; Acinetobacter Infections; Bacteremia; Community-Acquired Infections; Female; Humans; Meropenem; Middle Aged; Thienamycins
PubMed: 26689082
DOI: 10.3201/eid2201.151298