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Current Microbiology Nov 2019A novel Gram-stain-negative, motile, and facultative anaerobic coccus, strain ChDC F240 was isolated from human subgingival dental plaque of a gingivitis lesion. The...
A novel Gram-stain-negative, motile, and facultative anaerobic coccus, strain ChDC F240 was isolated from human subgingival dental plaque of a gingivitis lesion. The phylogenetic analysis based on the 16S ribosomal RNA gene (16S rDNA) sequence showed that the strain belonged to the genus Lautropia. 16S rDNA of strain ChDC F240 had the highest similarity to that of Lautropia mirabilis ATCC 51599 (98.8%). Major cellular fatty acids of strain ChDC F240 were C (43.9%) and C/C (38.1%). Draft genome of the strain was 3,834,139 bp in length and the G+C content was 65.0 mol%. Average nucleotide identity and genome-to-genome distance values between strain ChDC F240 and L. mirabilis ATCC 51599 were 81.99% and 28.50% (26.1-30.9%), respectively. These results reveal that strain ChDC F240 is a novel species within the genus Lautropia, for which the name Lautropia dentalis sp. nov. is proposed; type strain is ChDC F240 (= KCOM 2505 = JCM 33297).
Topics: Bacterial Typing Techniques; Base Composition; Burkholderiaceae; DNA, Bacterial; DNA, Ribosomal; Dental Plaque; Fatty Acids; Genome, Bacterial; Gingivitis; Humans; Phylogeny; RNA, Ribosomal, 16S
PubMed: 31446477
DOI: 10.1007/s00284-019-01761-1 -
BMC Infectious Diseases Jul 2022Campylobacter rectus is a gram-negative rod, and Parvimonas micra is a gram-positive coccus, both of which are oral anaerobes that cause chronic periodontitis. Chronic...
Femoral osteomyelitis caused by oral anaerobic bacteria with mixed bacteremia of Campylobacter rectus and Parvimonas micra in a chronic periodontitis patient: a case report.
BACKGROUND
Campylobacter rectus is a gram-negative rod, and Parvimonas micra is a gram-positive coccus, both of which are oral anaerobes that cause chronic periodontitis. Chronic periodontitis can cause bacteremia and systemic diseases, including osteomyelitis. Hematogenous osteomyelitis caused by anaerobic bacteria is uncommon, and to date, there have been no reports of mixed bacteremia with C. rectus and P. micra. Here, we report the first case of osteomyelitis of the femur caused by anaerobic bacteria with mixed bacteremia of C. rectus and P. micra caused by chronic periodontitis.
CASE PRESENTATION
A 75-year-old man with chronic periodontitis, hyperuricemia, and benign prostatic hyperplasia was admitted to the hospital with a fracture of the left femur. The patient had left thigh pain for 4 weeks prior to admission. Left femoral intramedullary nail fixation was performed, and a large amount of abscess and necrotic tissue was found intraoperatively. The cultures of abscess specimens were identified as P. micra, Fusobacterium nucleatum, and C. rectus. C. rectus and P. micra were also isolated from blood cultures. C. rectus was identified by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and 16 S ribosomal RNA sequencing. Sulbactam-ampicillin was administered for approximately 1 month, after which it was replaced by oral clavulanic acid-amoxicillin for long-term suppressive treatment.
CONCLUSIONS
Only five cases of bloodstream infection with C. rectus have been reported, and this is the first report of mixed bacteremia with P. micra. Clinicians should consider that chronic periodontitis caused by rare oral anaerobic bacteria can cause systemic infections, such as osteomyelitis.
Topics: Abscess; Aged; Bacteremia; Bacteria, Anaerobic; Campylobacter rectus; Chronic Periodontitis; Femur; Firmicutes; Humans; Male; Osteomyelitis; Peptostreptococcus
PubMed: 35836203
DOI: 10.1186/s12879-022-07573-2 -
Journal of Microbiology, Immunology,... Oct 2016Veillonella parvula, an anaerobic, Gram-negative coccus is part of the normal flora of the oral, gastrointestinal, respiratory, and genitourinary tracts in humans and... (Review)
Review
Veillonella parvula, an anaerobic, Gram-negative coccus is part of the normal flora of the oral, gastrointestinal, respiratory, and genitourinary tracts in humans and animals. We herein present a case of epidural abscess caused by V. parvula in a 68-year-old man with sinus squamous cell carcinoma who presented with a 3-week history of low back pain. Blood and pus cultures were positive for Veillonella spp. After sequencing of the 16S ribosomal DNA, the pathogen was identified as V. parvula. Surgical debridement was performed following which the patient received intravenous administration of amoxicillin/clavulanate. To our knowledge, there are only seven reported cases of spinal infection caused by Veillonella spp. and these are reviewed here.
Topics: Adult; Aged; Amoxicillin; Anti-Bacterial Agents; Carcinoma, Squamous Cell; Clavulanic Acid; Epidural Abscess; Female; Gram-Negative Bacterial Infections; Humans; Male; Middle Aged; RNA, Ribosomal, 16S; Veillonella
PubMed: 25066704
DOI: 10.1016/j.jmii.2014.05.002 -
International Journal of Systematic and... May 2019A Gram-stain-positive, non-spore-forming, catalase-positive and facultatively anaerobic coccus, designated ZY16052, was isolated from mesenteric lymph nodes of a sick...
A Gram-stain-positive, non-spore-forming, catalase-positive and facultatively anaerobic coccus, designated ZY16052, was isolated from mesenteric lymph nodes of a sick piglet in Kunming, Yunnan Province, PR China and its taxonomic position was studied by following a polyphasic approach. Optimal growth was observed at 37 °C, pH 8.0 and 2 % NaCl (w/v) on Columbia agar. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain ZY16052 formed a separated evolutionary lineage from recognized genera of the family Aerococcaceae and shared low similarity to its closest related species Facklamiasourekii (93.8 %) and Ignavigranum ruoffiae (93.4 %). Phylogenetic analysis based on the housekeeping gene recA indicated that strain ZY16052 represented a deep and distinct evolutionary lineage, and was well separated from all genera in the family Aerococcaceae, with very low sequence similarity(≤73.2 %). Sequence analysis based on the housekeeping gene rpoA indicated that strain ZY16052 shared very low similarity ≤77.0 % to related genera. The genomic OrthoANI values between strain ZY16052 and type species of related genera in the family Aerococcaceae and species in the genus Facklamia were ≤67.77 and ≤68.11 %, respectively. The genomic G+C content was 42.3 mol%. The predominant fatty acids (>5 %) were C16 : 0, C18 : 1ω9c, C14 : 0 and summed feature 5 (C18 : 2ω6,9c and/or C18 : 0 ante). The major polar lipids were digalactosyldiacylglycerol, phosphatidylglycerol, diacylglycerols, triacylglycerol and phosphatidic acid. The peptidoglycan contained the amino acids lysine, glycine, alanine and glutamic acid, which is characteristic of peptidoglycan type A1a. Based on the phylogenetic and phenotypic evidence, we propose that the unknown bacterium be classified as Suicoccus acidiformans gen. nov., sp. nov. The type strain of Suicoccus acidiformans is ZY16052 (=CCTCC AB 2017017=DSM 105755).
Topics: Aerococcaceae; Animals; Bacterial Typing Techniques; Base Composition; China; DNA, Bacterial; Fatty Acids; Genes, Bacterial; Lymph Nodes; Peptidoglycan; Phospholipids; Phylogeny; RNA, Ribosomal, 16S; Sequence Analysis, DNA; Swine
PubMed: 30882297
DOI: 10.1099/ijsem.0.003340 -
Anales Del Sistema Sanitario de Navarra Dec 2017Rothia mucilaginosa (R. mucilaginosa), formerly named Stomatococcus mucilaginosus, is a facultatively anaerobic, encapsulated gram-positive coccus, which forms part of...
Rothia mucilaginosa (R. mucilaginosa), formerly named Stomatococcus mucilaginosus, is a facultatively anaerobic, encapsulated gram-positive coccus, which forms part of the normal oropharyngeal and is rarely considered to be a pathogen in immunocompetent patients, although it can produce, on rare occasions, serious infections like bacteremia, endocarditis and respiratory infections; such as pneumonia, pleural empyema or superinfection of bronchiectasis. We present the case of a 74-year-old male diagnosed with right basal pneumonia of torpid evolution with a poor initial response to different antibiotics, with clinical and radiological worsening and the appearance of bilateral bronchopneumonia with pseudonodular images. R. mucilaginosa in pure culture was isolated in three sputum cultures and in bronchial suction. The patient was finally treated with Linezolid with a good clinical response and normalisation of the thorax radiography, confirming the disappearance of R. mucilaginosa in subsequent sputum cultures. As there are few documented cases of pneumonia due to R. mucilaginosa, we believe that presenting this case will be of interest.
Topics: Aged; Bronchopneumonia; Gram-Positive Bacterial Infections; Humans; Male; Micrococcaceae
PubMed: 29149111
DOI: 10.23938/ASSN.0090 -
Anaerobe Dec 2021Peptostreptococcus anaerobius is a gram-positive anaerobic coccus (GPAC) found in the gastrointestinal and vaginal microbiota. The organism is mainly found in... (Review)
Review
Peptostreptococcus anaerobius: Pathogenicity, identification, and antimicrobial susceptibility. Review of monobacterial infections and addition of a case of urinary tract infection directly identified from a urine sample by MALDI-TOF MS.
Peptostreptococcus anaerobius is a gram-positive anaerobic coccus (GPAC) found in the gastrointestinal and vaginal microbiota. The organism is mainly found in polymicrobial and scarcely in monobacterial infections such as prosthetic and native endocarditis. Anaerobic bacteria have rarely been reported as the cause of urinary tract infection (UTI). Although GPAC are susceptible to most antimicrobials used against anaerobic infections, P. anaerobius has shown to be more resistant. Herein, we report a case of UTI caused by P. anaerobius from a 62-year-old man with a history of urological disease. Surprisingly, the microorganism was directly identified by Matrix-Assisted Laser Desorption-Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS) from the urine sample. The isolate was successfully identified by phenotypic methods, MALDI-TOF MS, and 16S rRNA gene sequencing. P. anaerobius showed no β-lactamase-producing activity, was resistant to penicillin, ampicillin, ciprofloxacin and levofloxacin, and displayed intermediate susceptibility to ampicillin-sulbactam and amoxicillin-clavulanic acid. Successful treatment was achieved with oral amoxicillin-clavulanic acid. Antimicrobial susceptibility testing (AST) should be performed on P. anaerobius isolates due to their unpredictable AST patterns and because empirically administered antimicrobial agents may not be active. This report shows that MALDI-TOF MS, directly used in urine specimens, may be a quick option to diagnose UTI caused by P. anaerobius or other anaerobic bacteria. This review is a compilation of monobacterial infections caused by P. anaerobius published in the literature, their pathogenicity, identification, and data about the antimicrobial susceptibility of P. anaerobius.
Topics: Anti-Infective Agents; Bacterial Typing Techniques; Disease Susceptibility; Gram-Positive Bacterial Infections; Humans; Male; Microbial Sensitivity Tests; Middle Aged; Molecular Typing; Peptostreptococcus; RNA, Ribosomal, 16S; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Treatment Outcome; Urinary Tract Infections
PubMed: 34626800
DOI: 10.1016/j.anaerobe.2021.102461 -
International Journal of Systematic and... Feb 2020An obligately anaerobic, Gram-stain-positive and coccus-shaped bacterium, designated strain YH-panp20, was isolated from pig faeces. Phylogenetic analysis based on 16S...
An obligately anaerobic, Gram-stain-positive and coccus-shaped bacterium, designated strain YH-panp20, was isolated from pig faeces. Phylogenetic analysis based on 16S rRNA gene sequencing indicated that the isolate belongs to the family , and is most closely related to KCTC 15517 (93.5 % sequence similarity), followed by KCTC 5815 (92.2 %), KCTC 15521 (90.2 %) and KCTC 5969 (89.6 %). Average nucleotide identity values between YH-panp20 and its closest relatives were lower than 71 %. The G+C content of the isolate was 38.4 mol%, and its cell-wall peptidoglycan was found to be of A1γ type and contained -diaminopimelic acid. The predominant fatty acids were C 9, C DMA and C. The major end-products of glucose fermentation were lactate, acetate and formate. Therefore, based on the phenotypic, phylogenetic and chemotaxonomic properties, a novel genus and species, gen. nov., sp. nov., is proposed for isolate YH-panp20 (=KCTC 15747=JCM 32769).
Topics: Animals; Bacterial Typing Techniques; Base Composition; Cell Wall; DNA, Bacterial; Diaminopimelic Acid; Fatty Acids; Feces; Fermentation; Firmicutes; Gram-Positive Rods; Peptidoglycan; Phylogeny; RNA, Ribosomal, 16S; Republic of Korea; Sequence Analysis, DNA; Swine
PubMed: 31702538
DOI: 10.1099/ijsem.0.003803 -
BMJ Case Reports May 2020Necrotising fasciitis is a life-threatening condition characterised by inflammation, affecting the soft tissues, which spreads within a fascial plane. Skin changes can...
Necrotising fasciitis is a life-threatening condition characterised by inflammation, affecting the soft tissues, which spreads within a fascial plane. Skin changes can be delayed and can often go unnoticed. The condition arises from a bacterial infection, commonly being of polymicrobial aetiology. We describe an uncommon case of necrotising fasciitis caused by , an anaerobic coccus, in a 40-year-old patient with diabetes. is a Gram-positive anaerobic coccus, which was previously known as The bacteria is found in the normal flora of the urogenital tract. The bacteria is associated with severe infections such as native valve endocarditis, paravalvular abscess around a bioprosthetic valve, purulent pericarditis complicated by mediastanitis, meningitis after pneumonia and necrotising pneumonia complicated by pyopneumothorax. There have been no cases in the literature describing necrotising fasciitis of the abdominal wall caused by .
Topics: Abdominal Wall; Adult; Fasciitis, Necrotizing; Firmicutes; Humans; Male
PubMed: 32467123
DOI: 10.1136/bcr-2020-235115 -
Case Reports in Infectious Diseases 2015Streptococcus suis is a facultative anaerobic, Gram-positive coccus that can cause severe disease to both pigs and humans. Its zoonotic potential was first recognized in...
Streptococcus suis is a facultative anaerobic, Gram-positive coccus that can cause severe disease to both pigs and humans. Its zoonotic potential was first recognized in 1968 when the first human case of meningitis was reported in Denmark. Since then, over 1600 human cases have been reported worldwide, the vast majority of which originated in Southeast Asia, and, thus, S. suis has been fairly characterized as an emerging pathogen. Infection in humans presents most commonly as bacteremia and/or meningitis while less common clinical manifestations such as endocarditis and septic arthritis can occur. S. suis infection is extremely uncommon in Greece and this is the third human case to be reported. Correct identification is of importance for optimization of antimicrobial treatment and epidemiological monitoring.
PubMed: 26435860
DOI: 10.1155/2015/505834 -
Anaerobe Feb 2023Murdochiella asaccharolytica is a Gram-positive anaerobic coccus firstly isolated from wound specimens, since then, it was very rarely isolated from human infections. We...
Murdochiella asaccharolytica is a Gram-positive anaerobic coccus firstly isolated from wound specimens, since then, it was very rarely isolated from human infections. We report the first case of bloodstream infection due to this bacterium in a 90 years-old-man. The patient had a history of B-cell lymphoma and presented with pain and induration of the base of penis along with fever. A blood culture was obtained yielding M. asaccharolytica in pure culture. Only resistance to clindamycin was demonstrated. Treatment with metronidazole was started and the clinical evolution improved with antimicrobial treatment, being discharged after 6 days.
Topics: Male; Humans; Aged, 80 and over; Bacteria, Anaerobic; Bacterial Infections; Bacteremia; Metronidazole; Bacteria; Gram-Positive Cocci; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
PubMed: 36649886
DOI: 10.1016/j.anaerobe.2023.102697