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Open Medicine (Warsaw, Poland) 2022Lung cancer (LC) is a prevailing primary tumor in the lung. lncRNA non-coding RNA activated by DNA damage (NORAD) is a popular target in human cancers. This experiment...
Lung cancer (LC) is a prevailing primary tumor in the lung. lncRNA non-coding RNA activated by DNA damage (NORAD) is a popular target in human cancers. This experiment is designed to probe the mechanism of lncRNA in LC progression. NORAD expression in normal lung epithelial cells and LC cells was examined and then silenced to assess its effect on LC cell proliferation, invasion, and migration. Subcellular localization of NORAD was analyzed through online databases and then corroborated by fractionation of nuclear and cytoplasmic RNA assay. The target binding relations between NORAD and miR-28-3p and between miR-28-3p and E2F2 were verified. Eventually, LC cells with NORAD silencing were transfected with miR-28-3p inhibitor or pcDNA3.1-E2F2 to measure LC cell proliferation, invasion, and migration. NORAD was overexpressed in LC cells and NORAD knockout led to suppressed LC cell proliferation, invasion, and migration. Besides, NORAD targeted miR-28-3p and miR-28-3p targeted E2F2 transcription. Inhibiting miR-28-3p or overexpressing E2F2 could both annul the inhibitory role of si-NORAD in LC cell proliferation, invasion, and migration. Generally, our findings demonstrated that NORAD competitively bound to miR-28-3p with E2F2, to promote LC cell progression.
PubMed: 36245705
DOI: 10.1515/med-2022-0538 -
Chemosphere Apr 2021Valorization of waste phytomass into valuable components provide new functionality to these biowastes and annul problems associated with their safe disposal. In this...
Valorization of waste phytomass into valuable components provide new functionality to these biowastes and annul problems associated with their safe disposal. In this study, date palm (Phoenix dactylifera) coir (DPC) waste was tested for its toxic hexavalent chromium (Cr(VI)) ions biosorption. The DPC biosorbent was subjected to SEM, EDX, FTIR, TGA and N adsorption/desorption characterization studies. Results showed that the cellulose-rich DPC surface contained mesopores with a wide number of functional groups and possessed suitable surface attributes for Cr(VI) ions sequestration. Batch biosorption tests established the Cr(VI) ions sequestration potential of the DPC biosorbent with a maximum chromium removal efficiency of 87.2% for a 100 ppm initial feed concentration at pH 2, dosage 0.3 g, temperature 30 °C, contact time 60 min and agitation speed 100 rpm. Langmuir isotherm fitted well (R = 0.9955) with the experimental data while the kinetic analysis showed that Cr(VI) ions sequestration by DPC followed the pseudo-second order model. Biosorption thermodynamics revealed the exothermic nature and low-temperature preference for the effective binding of chromium ions on DPC. Regeneration of the biosorbent using NaOH wash showed a nearly steady Cr(VI) ions removal efficiency (with a loss <10%) by the DPC till four recycle runs. Economic analysis showed a very low production cost of $1.09/kg for the DPC biosorbent with a total cost of $4.36/m for a scale-up batch process wastewater treatment plant. Thus, a low-cost, effectual and sustainable biosorbent for effective treatment of Cr(VI) ions polluted water streams has been reported.
Topics: Adsorption; Chromium; Hydrogen-Ion Concentration; Kinetics; Lignin; Phoeniceae; Thermodynamics; Water Pollutants, Chemical; Water Purification
PubMed: 33187657
DOI: 10.1016/j.chemosphere.2020.128809 -
Journal of Leukocyte Biology May 2022Ischemic stroke (IS) can cause disability and death, and microglia as the immune component of the CNS can release inflammatory factors and participate in blood-brain...
Ischemic stroke (IS) can cause disability and death, and microglia as the immune component of the CNS can release inflammatory factors and participate in blood-brain barrier (BBB) dysfunction. This study aimed to investigate the effects of long noncoding RNA (lncRNA) SNHG8 on microglia activation and BBB permeability in IS. A rat model of permanent middle cerebral artery occlusion (p-MCAO) and a cell model of oxygen and glucose deprivation (OGD) in microglia were established, followed by evaluation of neurobehavioral function, BBB permeability, brain edema, and pathologic changes of microglia in brain tissue. The activation status of microglia and expressions of inflammatory factors were detected. Cell viability and integrity of microglia membrane were assessed. The downstream microRNA (miR), gene, and pathway of SNHG8 were analyzed. LncRNA SNHG8 was down-regulated in MCAO rats. Overexpression of SNHG8 improved the neural function defect, reduced brain water content, BBB permeability, brain tissue damage and inflammation, and inhibited microglia activation. In OGD-induced microglia, overexpression of SNHG8 or miR-449c-5p down-regulation increased cell viability and decreased lactate dehydrogenase activity. Moreover, SNHG8 sponged miR-449c-5p to regulate SIRT1. Overexpression of SNHG8 increased the expression of SIRT1 and FoxO1. MiR-449c-5p mimic could annul the effect of SNHG8 overexpression on ischemic microglia. Collectively, SNHG8 inhibits microglia activation and BBB permeability via the miR-449c-5p/SIRT1/FoxO1 pathway, thus eliciting protective effects on ischemic brain injury.
Topics: Animals; Blood-Brain Barrier; Brain Ischemia; Glucose; Ischemic Stroke; MicroRNAs; Microglia; Nerve Tissue Proteins; Permeability; RNA, Long Noncoding; Rats; Sirtuin 1
PubMed: 34585441
DOI: 10.1002/JLB.1A0421-217RR -
Environmental Science and Pollution... Nov 2023The rapid development of the Belt and Road Initiative (BRI) has led to severe air pollution dominated by PM2.5 concentrations which can cause a profound negative impact...
The rapid development of the Belt and Road Initiative (BRI) has led to severe air pollution dominated by PM2.5 concentrations which can cause a profound negative impact on human health and economic activity. This problem poses a critical environmental challenge to efficiently handling large-scale spatial-temporal PM2.5 data in this extended region. Functional data analysis (FDA) technique offers powerful tools that have the potential to enhance the analysis of spatial distributions and temporal dynamic changes in high-dimensional pollution data. However, modeling the spatial-temporal variability of PM2.5 concentrations by FDA remains unrevealed in the BRI region. To address this research gap, our study aimed to achieve two main objectives: first, to model the spatial-temporal dynamic variability of PM2.5 in 125 BRI nations (1998-2021), and second, to identify the underlying clusters behind the variations. We employed the recently developed functional adaptive density peak (FADP) clustering approach to solve the current problem. The proposed method is based on the joint use of functional principal components (FPCs) and functional cluster analyses. The main results are as follows: (i) The first three FPCs almost captured 99% of the total variations involving all valuable information on PM2.5 concentrations. (ii) PM2.5 pollution was highly concentrated in the developing countries (Pakistan, Bangladesh, and Nigeria) and the developed countries (Arabian Gulf countries: Qatar, United Arab Emirates, Bahrain, Saudi Arabia, Oman), and the least developed countries (Yemen and Chad). (iii) Three optimal clusters were identified and thus classified the PM2.5 into three distinct degrees of pollution: severe, moderate, and light. (iv) Cluster 1 had a severe pollution effect degree with a high rate of change, and it covered the Arabian Peninsula countries, African countries (Cameroon, Egypt, Gambia, Mali, Mauritania, Nigeria, Sudan, Senegal, Chad), Bangladesh, and Pakistan. (v) About 62 BRI countries belonged to cluster 2 showing a light pollution degree with annul average of less than 20 [Formula: see text]; this pointed out that the PM2.5 concentration remains stable in the cluster 2-related countries. The findings of this research would benefit governments and policymakers in preventing and controlling PM2.5 pollution exposure in BRI. Furthermore, this research could pay attention to sustainable development goals and the vision of the Green BRI policy.
Topics: Humans; Air Pollution; Particulate Matter; Bahrain; Oman; Nigeria; Economic Development
PubMed: 37798523
DOI: 10.1007/s11356-023-30048-z -
Engineering (Beijing, China) Feb 2017Knee osteoarthritis (OA) is the most common form of arthritis worldwide. The incidence of this disease is rising and its treatment poses an economic burden. Two early...
Knee osteoarthritis (OA) is the most common form of arthritis worldwide. The incidence of this disease is rising and its treatment poses an economic burden. Two early targets of knee OA treatment include the predominant symptom of pain, and cartilage damage in the knee joint. Current treatments have been beneficial in treating the disease but none is as effective as total knee arthroplasty (TKA). However, while TKA is an end-stage solution of the disease, it is an invasive and expensive procedure. Therefore, innovative regenerative engineering strategies should be established as these could defer or annul the need for a TKA. Several biomaterial and cell-based therapies are currently in development and have shown early promise in both preclinical and clinical studies. The use of advanced biomaterials and stem cells independently or in conjunction to treat knee OA could potentially reduce pain and regenerate focal articular cartilage damage. In this review, we discuss the pathogenesis of pain and cartilage damage in knee OA and explore novel treatment options currently being studied, along with some of their limitations.
PubMed: 35392109
DOI: 10.1016/j.eng.2017.01.003 -
BMC Public Health Jan 2015Lifestyle diseases could be prevented and controlled by disseminating health knowledge. This study explored the health knowledge awareness and the impact factors of...
BACKGROUND
Lifestyle diseases could be prevented and controlled by disseminating health knowledge. This study explored the health knowledge awareness and the impact factors of health knowledge awareness, and the way people received health knowledge in western China.
METHODS
We undertook a cross-sectional survey in 8 counties, 24 townships and 72 villages from July 2011 to April 2012 in Inner Mongolia, Xinjiang, Chongqing and Qinghai in China. Collected data, which were publicly available, consisted of two parts, namely, socio-demographic information and the 1466 corresponding rural residents' awareness and the approach of health knowledge. Analysis of Variance (ANOVA) was used to explore the impact factors of health knowledge awareness. Multiple linear regressions was then applied to examine the potential predictors of health knowledge awareness.
RESULTS
Four predictors-age (negative factor), educational level (positive factor), distance from home to the nearest medical institution (negative factor) and annul disposable household income (negative factor) were in the final liner regression model (p < 0.05). The results showed that awareness of health knowledge associated with risk factors was the highest (58.85%). The highest awareness rate of health knowledge is the title "Whether secondhand smoke is harmful to myself" (69.78%) and the lowest title is "Whether eating with hepatitis B patients will be infected Hepatitis B" (21.69%). The main way to receive health knowledge was traditional way such as doctors (80.45%). About more than half of the residents received health knowledge through television, video, newspaper and magazines (65.78%), family members, neighbors (67.38%) and the village health bulletin boards (53.16%).
CONCLUSION
Health knowledge awareness of rural residents was quite low and the way of receiving health knowledge was simple and traditional. One of the critical factors was education level. Direct results showed that lower income families always obtained higher health knowledge level than the rich families. The main way to receive health knowledge was traditional ways. In the process of health education, different means of education should be adopted for different groups so as to achieve ideal effect. Potential interventions may be different from education process which should be adapted to different income level families.
Topics: Adult; Age Factors; Aged, 80 and over; Awareness; China; Cross-Sectional Studies; Educational Status; Female; Health Behavior; Health Knowledge, Attitudes, Practice; Humans; Life Style; Male; Middle Aged; Risk Factors; Rural Population; Socioeconomic Factors; Surveys and Questionnaires
PubMed: 25637079
DOI: 10.1186/s12889-015-1393-2 -
Environmental Science and Pollution... May 2023Selective catalytic reduction (SCR) has been one of the most efficient and widely used technologies to remove nitrogen oxides (NO). SCR research has developed rapidly in...
Selective catalytic reduction (SCR) has been one of the most efficient and widely used technologies to remove nitrogen oxides (NO). SCR research has developed rapidly in recent years, which can be reflected by the dramatic increase of related academic publications. Herein, based on the 10,627 documents from 2001 to 2020 in Web of Science, the global research landscape and hotspots in SCR are investigated based on a comprehensive bibliometric analysis. The results show that SCR research has developed positively; the annul number of articles increase sharply from 246 in 2001 to 1092 in 2020. People's Republic of China and Chinese Academy of Sciences are the most productive country and institution, respectively. The global collaboration is extensive and frequent, while People's Republic of China and USA have the most frequent research cooperation. Applied Catalysis B-Environmental is the leading publication source with 711 records. Five major research areas on SCR are identified and elaborated, including catalyst, reductant, deactivation, mechanism, and others. Zeolite is the most widely studied SCR catalyst, while copper, silver, platinum, and iron are the most popular metal elements in catalyst. Ammonia (NH) is dominated among various SCR reductants, while hydrocarbon reductant has gained more attention. Sulfur dioxide (SO) and vapor are the two most concerned factors leading to catalyst deactivation, and catalyst regeneration is also an important research topic. Density functional theory (DFT), in situ diffuse reflectance infrared Fourier transform spectroscopy (DRIFTS), Fourier transform infrared spectroscopy (FTIR), X-ray photoelectron spectroscopy (XPS), and kinetics are the most widely used methods to conduct mechanism study. The studies on "low temperature," "atomic-scale insight," "elemental mercury," "situ DIRFTS investigation," "arsenic poisoning," "SPOA-34," "Cu-CHA catalyst," "TiO catalyst," and "Ce catalyst" have been the hotspots in recent years.
Topics: Humans; Oxidation-Reduction; Reducing Agents; Nitrogen Oxides; Ammonia; Catalysis
PubMed: 37081369
DOI: 10.1007/s11356-023-26993-4 -
The Science of the Total Environment Aug 2020Bacteria are critical ecosystem drivers in both aquatic and terrestrial ecosystems. However, our understanding of the mechanisms generating and maintaining biodiversity...
Bacteria are critical ecosystem drivers in both aquatic and terrestrial ecosystems. However, our understanding of the mechanisms generating and maintaining biodiversity on large spatial scales remains limited, especially mechanisms involving rare taxa in soil ecosystems. In the present study we took paddy soils in China as model ecosystems and studied the ecological diversity and assembly mechanisms of both the rare and abundant bacterial subcommunities. We collected 339 paddy soil samples from 113 sites across 19 Chinese provinces that span distances of up to 3869 km. The bacterial community was characterized by high-throughput sequencing of the 16S rRNA gene. The α-diversity of rare and abundant subcommunities showed opposite quadratic correlations with the key environmental factor soil pH. Rare taxa exhibited a stronger distance-decay relationship than the abundant subcommunity. Moreover, deterministic selection processes dominated in the assembly of the abundant subcommunity while stochastic processes dominated in that of the rare subcommunity based on both variation partitioning analysis and the phylogenetic null model. Soil pH was also the main deterministic factor driving the geographical distributions of both the rare and abundant subcommunity. Besides, mean annul temperature and soil texture were also found to be important factors affecting the biogeography and diversity patterns of abundant and rare subcommunities. These results indicate that the mechanisms generating and maintaining the diversity of the abundant and rare subcommunities were totally different in paddy soils, suggesting that these two subcommunities may respond differently to future environmental change.
Topics: Bacteria; China; Ecosystem; Oryza; Phylogeny; RNA, Ribosomal, 16S; Soil; Soil Microbiology
PubMed: 32402971
DOI: 10.1016/j.scitotenv.2020.139116 -
Pediatric Transplantation Dec 2021Progressive familial intrahepatic cholestasis type 1 (PFIC1) arises from biallelic variants in the ATP8B1 gene that annul FIC1 activity, resulting in progressive liver...
BACKGROUND
Progressive familial intrahepatic cholestasis type 1 (PFIC1) arises from biallelic variants in the ATP8B1 gene that annul FIC1 activity, resulting in progressive liver disease. Liver transplant (LT) is indicated in refractory disease; however, post-LT complications including worsening diarrhea and steatohepatitis progressing to fibrosis with graft loss have been reported. We aim to describe long-term outcomes of PFIC1 LT recipients at our center, focusing on the histological changes of the allografts.
METHODS
We assessed 7 PFIC1 patients post-LT at the Children's Hospital of Pittsburgh (CHP). All pre-transplant, explant, and sequential post-transplant pathology samples were reviewed. Continuous data are presented as the mean ± SD. We compared the pre- and post-transplant height and weight z-scores using Wilcoxon signed-rank test.
RESULTS
Seven (29% male) patients with PFIC1 received a LT (n = 6) or had post-LT care (n = 1) at CHP. Six had confirmed or suspected identical genetic. At a mean follow-up of 10.9 years, both patient survival and graft survival were 100%. Diarrhea persisted (n = 3) or newly developed (n = 4) in all patients after LT contributing to ongoing growth failure, with mean z-scores -2.63 (weight) and -2.98 (height) at follow-up. Histologically, allograft steatosis was common but was not accompanied by significant inflammation, ballooning, or fibrosis.
CONCLUSION
We show that extrahepatic disease persists and near-universal allograft steatosis occurs. However, at a mean follow-up period of over 10 years, no patients developed steatohepatitis or significant fibrosis, and both patient survival and graft survival are excellent.
Topics: Adolescent; Child; Child, Preschool; Cholestasis, Intrahepatic; Female; Humans; Infant; Liver Transplantation; Male; Pennsylvania
PubMed: 34339082
DOI: 10.1111/petr.14108 -
Chemistry (Weinheim An Der Bergstrasse,... Oct 2018The use of a template that bears pre-programmed receptor sites for selectively accommodating chromophores at given positions is an attractive approach for engineering...
The use of a template that bears pre-programmed receptor sites for selectively accommodating chromophores at given positions is an attractive approach for engineering artificial-light-harvesting systems. Indulging this line of thought, this work tackles the creation of tailored antenna architectures with yellow, red and blue chromophores, exploiting three dynamic covalent reactions simultaneously, namely disulfide exchange, acyl hydrazone, and boronic ester formations. The effect of various structural modifications, such as the chromophores as well as their spatial organization (distance, orientation, order) on the energy transfer within the antennas was studied by means of steady-state UV/Vis absorption and fluorescence spectroscopies. This systematic study allowed for a significant improvement of the energy-transfer efficiencies to a noticeable 22 and 15 % for the yellow and red donors, respectively, across the chromophores to the blue acceptor. Metadynamics simulations suggested that the conformational properties of the antennas are driven by intramolecular chromophoric stacking interactions that, upon forcing the α-helix to fold on itself, annul any effects deriving from the programming of the spatial arrangement of the receptor sides in the peptide backbone.
Topics: Boronic Acids; Disulfides; Energy Transfer; Esters; Fluorescent Dyes; Hydrazones; Hydrophobic and Hydrophilic Interactions; Light; Light-Harvesting Protein Complexes; Molecular Conformation; Molecular Dynamics Simulation; Peptides; Photosynthesis; Solvents
PubMed: 30133049
DOI: 10.1002/chem.201803205