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Molecular Medicine Reports Mar 2019The brain‑derived neurotrophic factor (BDNF) and c‑Jun NH 2‑terminal kinase (JNK) signaling pathways are therapeutic targets to prevent degeneration in the central...
The brain‑derived neurotrophic factor (BDNF) and c‑Jun NH 2‑terminal kinase (JNK) signaling pathways are therapeutic targets to prevent degeneration in the central nervous system. Dexamethasone (DXMS), a glucocorticoid, protects against vestibular brain injury, however, the molecular mechanisms have yet to be fully elucidated. To investigate whether the BDNF and JNK signaling pathways are involved in the protective effects of DXMS in rats with vestibular dysfunction, a rat model of severe vestibular deficits was established by middle ear injection of arsanilic acid (AA; 100 mg/ml; 0.05 ml). After 3 days, rat symptoms and behavior scores with vestibular disorders were detected. In brain tissues, histopathological alterations, cell apoptosis, expression levels and patterns of BDNF signaling pathway‑associated BDNF, tyrosine receptor kinase B (TrKB) and K+/Cl‑ cotransporter isoform 2 (KCC2), and the expression of apoptosis‑related cleaved‑caspase 3 and the JNK signaling pathway were detected. It was identified that DXMS relieved AA‑induced vestibular dysfunction, leading to improvement in rat behavior scores to normal levels, minimizing brain damage at the histopatholojnnkngical level, reducing cell apoptosis, enhancing the expression of BDNF, TrKB and KCC2, and downregulating cleaved‑caspase 3 and phosphorylated‑JNK1/2 in brain tissues. Together, these findings indicated the protective effect of DXMS on AA‑induced rat vestibular dysfunction, and that activating BDNF and inhibiting JNK singling pathways were the underlying mechanisms. In addition, with additional treatment of mifepristone (RU486), a specific glucocorticoid agonist, all the events elicited by DXMS mentioned above in the AA‑treated rat rats were reversed. In conclusion, DXMS was identified as a therapeutic agent targeting the BDNF and JNK singling pathways for AA‑induced rat vestibular dysfunction.
Topics: Animals; Arsanilic Acid; Brain; Brain-Derived Neurotrophic Factor; Caspase 3; Dexamethasone; MAP Kinase Signaling System; Male; RNA, Messenger; Rats, Sprague-Dawley; Receptor, trkB; Vestibular Diseases
PubMed: 30628712
DOI: 10.3892/mmr.2019.9835 -
The Science of the Total Environment Feb 2022Organoarsenic contaminants existing in water body threat human health and ecological environment due to insufficient bifunctional treatment technologies for...
Multifunctional capacity of CoMnFe-LDH/LDO activated peroxymonosulfate for p-arsanilic acid removal and inorganic arsenic immobilization: Performance and surface-bound radical mechanism.
Organoarsenic contaminants existing in water body threat human health and ecological environment due to insufficient bifunctional treatment technologies for organoarsenic degradation and inorganic arsenic immobilization. In order to safely and efficiently treat organoarsenic contaminants discharged into the aquatic environment, Co-Mn-Fe layered double hydroxide (CoMnFe-LDH) and Co-Mn-Fe layered double oxide (CoMnFe-LDO) were fabricated and employed as peroxymonosulfate (PMS) activator for organoarsenic degradation and inorganic arsenic immobilization, and p-arsanilic acid (p-ASA) was selected as target pollutant. Results demonstrated that the satisfactory removal of p-ASA (100.0%) in both CoMnFe-LDH/PMS and CoMnFe-LDO/PMS systems was obtained within 30 min, and substantial inorganic arsenic adsorption could be achieved (below 0.5 mg/L) in two systems with converting major inorganic arsenic species to arsenate. As XPS, ESR and quenching experiment revealed, the existence and generation of surface-bound radicals in two systems were identified. Based on density functional theory calculation and XPS analysis, the catalytic mechanism of CoMnFe-LDO/PMS system that PMS could be activated via direct electron transfer from adsorbed p-ASA was clarified, which differed from PMS activation via coupling with surface hydroxyl groups in CoMnFe-LDH/PMS system. Catalytic performance assessment under various critical operation parameters indicated that CoMnFe-LDH presented more stable ability of p-ASA removal in a wide pH range and complex aquatic environment. The recycle experiment demonstrated the excellent stability and reusability of CoMnFe-LDH(LDO). Besides, seven degradation products of p-ASA in CoMnFe-LDH/PMS system including phenolic compounds, azophenylarsonic acid, nitrobenzene and benzoquinne were identified by UV-Vis spectra and LC-TOF-MS analysis, and the corresponding degradation pathway was proposed. In summary, compared to CoMnFe-LDO/PMS, CoMnFe-LDH/PMS holds great promise for the development of an oxidation-adsorption process for efficient control of organoarsenic pollutant.
Topics: Arsanilic Acid; Arsenic; Humans; Hydroxides; Peroxides
PubMed: 34571222
DOI: 10.1016/j.scitotenv.2021.150379 -
The Science of the Total Environment Sep 2021p-arsanilic acid (p-ASA) is still widely applied as feed additive in many countries. Accompanied with chemical reactions in the environment, p-ASA will release more...
p-arsanilic acid (p-ASA) is still widely applied as feed additive in many countries. Accompanied with chemical reactions in the environment, p-ASA will release more toxic inorganic arsenic. In order to safely and efficiently treat p-ASA flow washing into the environment, iron encapsulated B/N-doped carbon nanotubes (Fe@C-NB) were fabricated and used as the catalyst for the degradation of p-ASA. The calcination temperature and the dose of the iron salt have significant effects on the structure and properties of the catalysts. We have produced a series of catalysts of the same type to facilitate the degradation of p-ASA. Under optimal conditions of material (Fe@C-NB) syntheses, both 95% degradation of p-ASA and 86% total arsenic immobilization can be obtained with oxidant (Peroxymonosulfate, PMS) and catalyst (Fe@C-NB) treatment after 60 min. The effects of oxidant types (peroxydisulfate (PDS), PMS, hydrogen peroxide (HO)), amount, initial solution pH, inorganic anion, and other reaction conditions were studied in the p-ASA removal. In this Fenton-like reaction, the Fe@C-NB exhibits high efficiency and excellent stability without complex preparation methods; besides, the advantages of short reaction time and natural reaction conditions in Fe@C-NB/PMS system will promote the practical application of Fenton-like.
PubMed: 33933762
DOI: 10.1016/j.scitotenv.2021.147152 -
Environmental Science and Pollution... Mar 2023As a typical wide band gap photocatalyst, titania (TiO) cannot use the visible light and has fast recombination rate of photogenerated electron-hole pairs. Simultaneous...
As a typical wide band gap photocatalyst, titania (TiO) cannot use the visible light and has fast recombination rate of photogenerated electron-hole pairs. Simultaneous introduction of erbium ion (Er) and graphene oxide (rGO) into TiO might overcome these two drawbacks. In this study, Er and rGO were co-doped on TiO to synthesize Er-rGO/TiO photocatalyst through a two-step sol-gel method. Based on the UV-visible diffuse reflectance spectra and photoluminescence spectrum, the introduction of Er and rGO increased the visible light absorption efficiency and enhanced the migration of photogenerated electron. Pure TiO has almost no photocatalytic activity for arsanilic acid (p-ASA) degradation under visible light irradiation. However, while doping with 2.0 mol% Er and 10.0 mol% rGO, the p-ASA could be completely degraded within 50 min by the Er-rGO/TiO photocatalyst under visible light irradiation, and most of produced inorganic arsenic was in situ removed by adsorption from the solution. The reactive oxygen species (ROS) reacting with p-ASA was determined and superoxide radical (O) and singlet oxygen (O) were the dominant ROS for the oxidation of p-ASA and arsenite. This work provides an approach of introducing Er and rGO to enhance the visible light photocatalytic efficiency of TiO.
Topics: Arsanilic Acid; Reactive Oxygen Species; Graphite
PubMed: 36525183
DOI: 10.1007/s11356-022-24627-9 -
Molecules (Basel, Switzerland) Dec 2021A sensitive electrochemical immunosensor was prepared for rapid detection of ASA based on arsanilic acid (ASA) monoclonal antibody with high affinity. In the preparation...
A sensitive electrochemical immunosensor was prepared for rapid detection of ASA based on arsanilic acid (ASA) monoclonal antibody with high affinity. In the preparation of nanomaterials, polyethyleneimine (PEI) improved the stability of the solution and acted as a reducing agent to generate reduced graphene oxide (rGO) with relatively strong conductivity, thereby promoting the transfer of electrons. The dual conductivity of rGO and silver nanoparticles (AgNPs) improved the sensitivity of the sensor. The synthesis of nanomaterials were confirmed by UV-Vis spectroscopy, X-ray diffraction, transmission electron microscopy and scanning electron microscopy. In the optimal experiment conditions, the sensor could achieve the detection range of 0.50-500 ng mL and the limit of detection (LOD) of 0.38 ng mL (S/N = 3). Moreover, the sensor exhibited excellent specificity and acceptable stability, suggesting that the proposed sensor possessed a good potential in ASA detection. Thus, the as-prepared biosensor may be a potential way for detecting other antibiotics in meat and animal-derived foods.
Topics: Arsanilic Acid; Biosensing Techniques; Electrochemical Techniques; Graphite; Immunoassay; Limit of Detection; Metal Nanoparticles; Nanocomposites; Polyethyleneimine; Reproducibility of Results; Silver
PubMed: 35011402
DOI: 10.3390/molecules27010172 -
Environmental Science & Technology May 2021As one of the extensively used feed additives in livestock and poultry breeding, -arsanilic acid (-ASA) has become an organoarsenic pollutant with great concern. For the...
As one of the extensively used feed additives in livestock and poultry breeding, -arsanilic acid (-ASA) has become an organoarsenic pollutant with great concern. For the efficient removal of -ASA from water, the combination of chemical oxidation and adsorption is recognized as a promising process. Herein, hollow/porous Mn-Fe-mixed oxide (MnFeO) nanocubes were synthesized and used in coupling with peroxymonosulfate (PMS) to oxidize -ASA and remove the total arsenic (As). Under acidic conditions, both -ASA and total As could be completely removed in the PMS/MnFeO process and the overall performance was substantially better than that of the Mn/Fe monometallic system. More importantly, an interface-promoted direct oxidation mechanism was found in the -ASA-involved PMS/MnFeO system. Rather than activate PMS to generate reactive oxygen species (i.e., SO, ·OH, and O), the MnFeO nanocubes first adsorbed -ASA to form a ligand-oxide interface, which improved the oxidation of the adsorbed -ASA by PMS and ultimately enhanced the removal of the total As. Such a direct oxidation process achieved selective oxidation of -ASA and avoidance of severe interference from the commonly present constituents in real water samples. After facile elution with dilute alkali solution, the used MnFeO nanocubes exhibited superior recyclability in the repeated -ASA removal experiments. Therefore, this work provides a promising approach for efficient abatement of phenylarsenical-caused water pollution based on the PMS/MnFeO oxidation process.
Topics: Arsanilic Acid; Arsenic; Oxidation-Reduction; Oxides; Peroxides; Water Pollutants, Chemical
PubMed: 33961405
DOI: 10.1021/acs.est.1c00386 -
Chemosphere Feb 2022Efficient and rapid removal of p-arsanilic acid (p-ASA) in water is very important in environmental protection and human health, however it is still a severe challenge...
Efficient and rapid removal of p-arsanilic acid (p-ASA) in water is very important in environmental protection and human health, however it is still a severe challenge in actual engineering. Herein, a novel sorbent (CF-PEI) was successfully fabricated by simply modifying the amphiphilic skin collagen fiber (CF) substrate with Polyethylenimine (PEI). The as-prepared CF-PEI exhibits high-efficiency adsorption for negatively charged p-ASA with aromatic rings due to the introduction of amino groups and the existence of hydrophobic bands, and the maximum adsorption capacity of CF-PEI for p-ASA was high up to 285.71 mg g. In addition, the adsorption mechanism of CF-PEI on p-ASA mainly includes electrostatic interaction, hydrogen bond and amphiphilicity. The multi-level all-fiber structure of CF makes it mainly focus on surface mass transfer with short mass transfer distance, and its capillary drainage effect can realize large flow and rapid separation. CF-PEI based on CF can realize the ability to separate low-concentration p-ASA with high flow rate and high efficiency. The effective processing volume was 12.5 L g when the separation flux reached as high as 9931.27 L m h. Notably, the p-ASA adsorbed on CF-PEI was almost completely eluted by NaOH (0.5 mol L). The adsorbent is convenient to prepare, recyclable, high in efficiency, and has a great application prospect in removing organic micro-pollutants.
Topics: Arsanilic Acid; Collagen; Humans; Water
PubMed: 34653489
DOI: 10.1016/j.chemosphere.2021.132542 -
Environmental Research Mar 2024Arsanilic acid (p-AsA), a prevalently used feed additive, is frequently detected in environment posing a great threat to humans. Potassium ferrate (Fe(VI)) was an...
Arsanilic acid (p-AsA), a prevalently used feed additive, is frequently detected in environment posing a great threat to humans. Potassium ferrate (Fe(VI)) was an efficient way to tackle arsenic contamination under acid and neutral conditions. However, Fe(VI) showed a noneffective removal of p-AsA under alkaline conditions due to its oxidation capacity attenuation. Herein, a magnetic iron-doped carbon nanotubes (F-CNT) was successfully prepared and further catalyzed Fe(VI) to remove p-AsA and total As species. The Fe(VI)/F-CNT system showed an excellent capability to oxidize p-AsA and adsorb total As species over an environment-related pH range of 6-9. The high-valent iron intermediates Fe(V)/Fe(IV) and the mediated electron-transfer played a significant part in the degradation of p-AsA according to the probes/scavengers experiments and galvanic oxidation process. Moreover, the situ formed iron hydroxide oxide and F-CNT significantly improved the adsorption capacity for total As species. The electron-donating groups (semiquinone and hydroquinone) and high graphitization of F-CNT were responsible for activating Fe(VI) based on the analysis of X-ray photoelectron spectroscopy (XPS). Density functional theory calculations and the detected degradation products both indicated that the amino group and the C-As bond of p-AsA were main reactive sites. Notably, Fe(VI)/F-CNT system was resistant to the interference from Cl, SO, and HCO, and could effectively remove p-AsA and total As species even in the presence of complex water matrix. In summary, this work proposed an efficient method to use Fe(VI) for degrading pollutants under alkaline conditions and explore a new technology for livestock wastewater advanced treatment.
Topics: Humans; Iron; Nanotubes, Carbon; Arsanilic Acid; Arsenic; Electrons; Oxidation-Reduction; Water Pollutants, Chemical; Water Purification; Ferric Compounds
PubMed: 38061591
DOI: 10.1016/j.envres.2023.117849 -
Journal of Hazardous Materials Nov 2020In this study, magnetic material based reduced graphene oxide (M-rGO) was prepared through co-precipitation and displayed high catalytic efficiency together with...
In this study, magnetic material based reduced graphene oxide (M-rGO) was prepared through co-precipitation and displayed high catalytic efficiency together with persulfate (PS) for simultaneous p-arsanilic acid (p-ASA) decomposition and arsenic removal. Linear sweep voltammetry and chronoamperometric measurements with M-rGO revealed that PS was effectively bound to M-rGO surface and probably formed charge transfer complex, in which M-rGO was pivotal in mediating facile electron transfer. The effects of pH, temperatures, anions, p-ASA concentration, PS, and M-rGO dosages on p-ASA decomposition were studied in the system. Excellent degradation of p-ASA was carried out at a wide range of pH values, which was unattainable by other Fenton-like processes. Under optimal conditions, M-rGO exhibited prominent removal of both p-ASA (98.8 %) and inorganic arsenic (89.8 %). M-rGO had reasonably excellent repeatability and stability, and 77.7 % p-ASA degraded in the third recovered catalyst. The advantages of environmental friendliness, short reaction time, and straightforward synthesis of M-rGO will facilitate the development of heterogeneous Fenton-like catalysts under neutral conditions.
PubMed: 32937710
DOI: 10.1016/j.jhazmat.2020.123032 -
The Science of the Total Environment Feb 2021Adsorption and desorption of p-arsanilic acid (p-ASA) and roxarsone (ROX) on six soil minerals, including hematite (α-FeO), goethite (α-FeOOH), ferrihydrite (Fe(OH)),...
Adsorption and desorption of p-arsanilic acid (p-ASA) and roxarsone (ROX) on six soil minerals, including hematite (α-FeO), goethite (α-FeOOH), ferrihydrite (Fe(OH)), aluminum oxide (α-AlO), manganese oxide (γ-MnO), and kaolinite, were studied, and the impact of solution matrices on their adsorption was systematically evaluated. Adsorption of p-ASA/ROX on the metal (hydro)oxide and clay minerals occurred quickly (mostly within 2 h), and could be well described by the pseudo second-order kinetic model. The apparent maximum adsorption capacities of α-FeO, α-FeOOH, Fe(OH), α-AlO, γ-MnO, and kaolinite (at an initial pH of 7.0) for p-ASA were 1.7, 0.9, 2.5, 0.08, 1.1, and 0.02 μmol/m, while those for ROX were 1.6, 0.7, 2.4, 0.1, 0.5, and 0.05 μmol/m, respectively. Besides adsorbing p-ASA/ROX, γ-MnO also caused their oxidation. Experimental results suggest that formation of inner-sphere complexes through the arsonic acid group is the primary mechanism for adsorption of p-ASA/ROX on iron (hydro)oxides and γ-MnO, while outer-sphere complexation plays a critical role in their adsorption on α-AlO and kaolinite. Adsorption of p-ASA/ROX on the metal (hydro)oxide and clay minerals was affected by solution pH, co-existing metal ions (Ca, Mg, Al, Cu, Fe, and Zn), oxyanions (HPO, HCO, and SO), and humic acid. The solid-to-liquid partition coefficients of p-ASA during the desorption from α-FeO, α-FeOOH, Fe(OH), α-AlO, γ-MnO, and kaolinite were 0.47, 2.69, 4.38, 0.03, 30.4, and 0.1 L/g, while those of ROX were 0.28, 1.68, 3.48, 0.02, 4.0, and 0.02 L/g, respectively. Agricultural soils with lower contents of organic carbon exhibited higher adsorption capacities towards p-ASA/ROX, which indicates that soil minerals play a key role in the adsorption of phenylarsonic acid compounds while organic matter could have strong inhibitory effect. These findings could help better understand and predict the transport and fate of p-ASA/ROX in surface soils with low contents of organic matter.
PubMed: 33229094
DOI: 10.1016/j.scitotenv.2020.143765