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Bioresources and Bioprocessing Jul 2023Bacterioruberin and its rare glycosylated derivatives are produced by Arthrobacter agilis as an adaptation strategy to low temperature conditions. The high antioxidant...
Bacterioruberin and its rare glycosylated derivatives are produced by Arthrobacter agilis as an adaptation strategy to low temperature conditions. The high antioxidant properties of bacterioruberin held great promise for different future applications like the pharmaceutical and food industries. Microbial production of bacterioruberin via a cost-effective medium will help increase its commercial availability and industrial use. The presented study aims to optimize the production of the rare C carotenoid bacterioruberin and its derivatives from the psychotrophic bacteria Arthrobacter agilis NP20 strain on a whey-based medium as a cost effective and readily available nutritious substrate. The aim of the study is extended to assess the efficiency of whey treatment in terms of estimating total nitrogen content in treated and untreated whey samples. The significance of medium ingredients on process outcome was first tested individually; then the most promising factors were further optimized using Box Behnken design (BBD). The produced carotenoids were characterized using UV-visible spectroscopy, FTIR spectroscopy, HPLC-DAD chromatography and HPLC-APCI-MS spectrometry. The maximum pigment yield (5.13 mg/L) was achieved after a 72-h incubation period on a core medium composed of 96% sweet whey supplemented with 0.46% MgSO & 0.5% yeast extract and inoculated with 6% (v/v) of a 24 h pre-culture (10 CFU/mL). The cost of the formulated medium was 1.58 $/L compared with 30.1 $/L of Bacto marine broth medium. The extracted carotenoids were identified as bacterioruberin, bis-anhydrobacteriouberin, mono anhydrobacterioruberin, and glycosylated bacterioruberin. The presented work illustrates the possibility of producing bacterioruberin carotenoid from Arthrobacter agilis through a cost-effective and eco-friendly approach using cheese whey-based medium.
PubMed: 38647623
DOI: 10.1186/s40643-023-00662-3 -
Chemosphere Nov 2022The viable and degradation potential of the strains which adhered to soil minerals are essential for eliminating organic pollutants from soil. Herein, the interaction...
The viable and degradation potential of the strains which adhered to soil minerals are essential for eliminating organic pollutants from soil. Herein, the interaction (growth, biofilm formation and survive) of Arthrobacter sp. DNS10, an atrazine degrading strain, with three kinds of typical soil minerals, such as montmorillonite, kaolinite and goethite, as well as the atrazine degradation gene (trzN) expression of the strain in the minerals system were studied. The results showed that montmorillonite had significant promotion effect on the growth of strain DNS10, followed by kaolinite, but goethite significantly inhibited the growth of strain DNS10. In contrast, goethite notably promoted the biofilm formation and there was less biofilm detected in montmorillonite containing system. The percentage of the survival bacteria in the biofilm that formed on montmorillonite, kaolinite and goethite was 53.8%, 40.8% and 28.2%. In addition, there were more reactive oxygen species (ROS) were detected in the cells that exposed to goethite than those of the cells exposed to kaolinite and montmorillonite. These results suggest that the electrostatic repulsion between kaolinite/montmorillonite and strain DNS10 prevents them from contacting each other and facilitates bacterial growth by allowing the strain to obtain more nutrients. Oppositely, the needle-like morphology of goethite might damage the strain DNS10 cell when they were combined by electrostatic attraction, and the goethite induced ROS also aggravate the cytotoxicity of goethite on strain DNS10. In addition, the relative transcription of trzN in the cells contacted with montmorillonite, kaolinite and goethite was 0.94-, 0.27- and 0.20- fold of the no mineral exposure treatment. Briefly, this research suggests that the minerals with different structure and/or physicochemical characteristics might cause various trend for the biofilm formation and degradation potential of the bacteria.
Topics: Arthrobacter; Atrazine; Bentonite; Biofilms; Iron Compounds; Kaolin; Minerals; Reactive Oxygen Species; Soil; Soil Pollutants
PubMed: 35940415
DOI: 10.1016/j.chemosphere.2022.135904 -
International Journal of Systematic and... Oct 2023A Gram-stain-positive, catalase-positive, non-motile bacteria, with a rod-coccus cycle (designated as EH-1B-1) was isolated from a soil sample from Union Glacier in...
A Gram-stain-positive, catalase-positive, non-motile bacteria, with a rod-coccus cycle (designated as EH-1B-1) was isolated from a soil sample from Union Glacier in Ellsworth Mountains, Antarctica. Strain EH-1B-1 had an optimal growth temperature of 28 °C and grew at pH 7-10. The major cellular fatty acids were anteiso-C, iso-C, C and anteiso-C. The G+C content based on the whole genome sequence was 63.1 mol%. Strain EH-1B-1 was most closely related to members of the genus , namely and . The strain grew on tryptic soy agar, Reasoner's 2A agar, lysogeny broth agar and nutrient agar. The average nucleotide identity and digital DNA-DNA hybridization values between strain EH-1B-1 and its closest reference type strains ranged from 78 to 88 % and from 20.9 to 36.3 %, respectively. Based on phenotypic, chemotypic and genotypic evidence, it is proposed that strain EH-1B-1 represents a novel species of , for which the name sp. nov. is proposed, with strain EH-1B-1 (RGM 3386=LMG 32961) as the type strain.
Topics: Fatty Acids; Arthrobacter; Phospholipids; Ice Cover; Antarctic Regions; Agar; Base Composition; Phylogeny; DNA, Bacterial; RNA, Ribosomal, 16S; Bacterial Typing Techniques; Sequence Analysis, DNA; Soil Microbiology; Vitamin K 2; Peptidoglycan; Soil
PubMed: 37861393
DOI: 10.1099/ijsem.0.006095 -
BMC Genomics Jun 2021The Arthrobacter group is a known set of bacteria from cold regions, the species of which are highly likely to play diverse roles at low temperatures. However, their...
BACKGROUND
The Arthrobacter group is a known set of bacteria from cold regions, the species of which are highly likely to play diverse roles at low temperatures. However, their survival mechanisms in cold regions such as Antarctica are not yet fully understood. In this study, we compared the genomes of 16 strains within the Arthrobacter group, including strain PAMC25564, to identify genomic features that help it to survive in the cold environment.
RESULTS
Using 16 S rRNA sequence analysis, we found and identified a species of Arthrobacter isolated from cryoconite. We designated it as strain PAMC25564 and elucidated its complete genome sequence. The genome of PAMC25564 is composed of a circular chromosome of 4,170,970 bp with a GC content of 66.74 % and is predicted to include 3,829 genes of which 3,613 are protein coding, 147 are pseudogenes, 15 are rRNA coding, and 51 are tRNA coding. In addition, we provide insight into the redundancy of the genes using comparative genomics and suggest that PAMC25564 has glycogen and trehalose metabolism pathways (biosynthesis and degradation) associated with carbohydrate active enzyme (CAZymes). We also explain how the PAMC26654 produces energy in an extreme environment, wherein it utilizes polysaccharide or carbohydrate degradation as a source of energy. The genetic pattern analysis of CAZymes in cold-adapted bacteria can help to determine how they adapt and survive in such environments.
CONCLUSIONS
We have characterized the complete Arthrobacter sp. PAMC25564 genome and used comparative analysis to provide insight into the redundancy of its CAZymes for potential cold adaptation. This provides a foundation to understanding how the Arthrobacter strain produces energy in an extreme environment, which is by way of CAZymes, consistent with reports on the use of these specialized enzymes in cold environments. Knowledge of glycogen metabolism and cold adaptation mechanisms in Arthrobacter species may promote in-depth research and subsequent application in low-temperature biotechnology.
Topics: Antarctic Regions; Arthrobacter; Base Composition; Comparative Genomic Hybridization; Genome, Bacterial
PubMed: 34078272
DOI: 10.1186/s12864-021-07734-8 -
Pathogens (Basel, Switzerland) Apr 2021is a Gram-positive, aerobic that is widely distributed in the environment worldwide. Little is known about infection and it is commonly mis-identified by culturing...
is a Gram-positive, aerobic that is widely distributed in the environment worldwide. Little is known about infection and it is commonly mis-identified by culturing with commercial kits. To date, only six cases of bacteremia caused by have been reported in the literature. Herein, we report a case of bacteremia in an immunocompromised host. In this case report, the results of antimicrobial susceptibility testing showed that this clinical isolate of is sensitive to vancomycin, teicoplanin, but resistant to penicillin, cephalosporin and ciprofloxacin. Additionally, whole genome sequencing analysis identified common subunits of the urease system.
PubMed: 33917709
DOI: 10.3390/pathogens10040443 -
International Journal of Systematic and... Dec 2018A novel Gram-stain-positive, strictly aerobic strain, NEAU-SA1, which showed a rod-coccus growth life cycle, was isolated from forest soil from Zhangjiajie, Hunan...
A novel Gram-stain-positive, strictly aerobic strain, NEAU-SA1, which showed a rod-coccus growth life cycle, was isolated from forest soil from Zhangjiajie, Hunan Province, China. The isolate grew at 10-40 °C (optimum 28 °C), at pH 5.0-10.0 (optimum pH 7.0) and in the presence of up to 5 % (w/v) NaCl, although NaCl was not required for growth. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain NEAU-SA1 belonged to the genus Arthrobacter and was closely related to Arthrobacter cupressi DSM 24664 (98.1 % similarity). Average nucleotide identity values between NEAU-SA1 and A. cupressi DSM 24664 were 88.91 and 87.41 % by ANIm and ANIb analysis, respectively. The in silico DNA-DNA hybridization value between strain NEAU-SA1 and A. cupressi DSM 24664 was 34.20 %, again indicating they belong to different taxa. The genomic DNA G+C content was 66.74 mol%. The major cellular fatty acids (>10 %) were anteiso-C15 : 0, anteiso-C17 : 0 and iso-C16 : 0. The polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and two unidentified glycolipids. The predominant menaquinone was MK-9(H2). The peptidoglycan type was A3α with an interpeptide bridge comprising l-Lys and l-Ala. Glucose, ribose and galactose were the whole-cell sugars. On the basis of morphological, physiological, biochemical and chemotaxonomic analysis, strain NEAU-SA1 was classified as representing a novel species in the genus Arthrobacter, for which the name Arthrobacter silvisoli sp. nov. is proposed. The type strain is NEAU-SA1 (=DSM 106716=CCTCC AB 2017271).
Topics: Arthrobacter; Bacterial Typing Techniques; Base Composition; Cell Wall; China; DNA, Bacterial; Fatty Acids; Forests; Glycolipids; Nucleic Acid Hybridization; Peptidoglycan; Phospholipids; Phylogeny; RNA, Ribosomal, 16S; Sequence Analysis, DNA; Soil Microbiology; Vitamin K 2
PubMed: 30351271
DOI: 10.1099/ijsem.0.003085 -
Archives of Microbiology Nov 2020A Gram-stain positive, motile, aerobic and rod-shaped strain (MIC A30) was isolated from river sediment in Yuantouzhu park, Wuxi City, China. Growth occurred at...
A Gram-stain positive, motile, aerobic and rod-shaped strain (MIC A30) was isolated from river sediment in Yuantouzhu park, Wuxi City, China. Growth occurred at 20-40 °C, at pH 6.0-9.0 and at 0-5.0% NaCl. Strain MIC A30 was moderately related to Arthrobacter liuii CGMCC 1.12778 (97.9%), Arthrobacter pokkalii (97.9%) and Arthrobacter globiformis NBRC 12137 (96.7%) by 16S rRNA analysis. The DNA-DNA relatedness values between strain MIC A30 and these reference strains were below 30%. The DNA G+C content was 63.1 mol%. Average nucleotide identity (ANI) and genome-to-genome distance (GGD) values between strain MIC A30 and A. liuii CGMCC 1.12778 were 60.34% and 29.39%, respectively. Quinone was identified as MK-9(H2). Major polar lipids were diphosphatidylglycerol and phosphatidylglycerol. Major fatty acids were iso-C, anteiso-C and anteiso-C. Whole-cell sugars were galactose, mannose and rhamnose. The cell wall peptidoglycan contained A4α peptidoglycan type with lysine as the diagnostic diamino acid. Based on several taxonomic results, strain MIC A30 is identified as a novel species in genus Arthrobacter, whose name is proposed as Arthrobacter sedimenti sp. nov. The type strain is MIC A30 (= KACC 19599 = CGMCC 1.13474).
Topics: Arthrobacter; Base Composition; China; DNA, Bacterial; Geologic Sediments; Nucleic Acid Hybridization; Peptidoglycan; RNA, Ribosomal, 16S; Rivers; Soil Microbiology; Species Specificity
PubMed: 32661667
DOI: 10.1007/s00203-020-01968-y -
Microbiology Resource Announcements Nov 2023London is a predicted temperate bacteriophage with siphovirus morphology infecting NRRL strain B-2880. Sequencing of the genome revealed a length of 43,599 bp...
London is a predicted temperate bacteriophage with siphovirus morphology infecting NRRL strain B-2880. Sequencing of the genome revealed a length of 43,599 bp comprising 69 predicted open-reading frames and no tRNA genes. It is categorized as a cluster AZ1 phage along with closely related actinobacteriophages Elezi, Eraser, and Niobe.
PubMed: 37906022
DOI: 10.1128/MRA.00819-23 -
International Journal of Systematic and... Jan 2018A novel Gram-stain-positive, strictly aerobic, non-endospore-forming bacterium, designated CAU 9143, was isolated from a hydric soil sample collected from Seogmo Island...
A novel Gram-stain-positive, strictly aerobic, non-endospore-forming bacterium, designated CAU 9143, was isolated from a hydric soil sample collected from Seogmo Island in the Republic of Korea. Strain CAU 9143 grew optimally at 30 °C, at pH 7.0 and in the presence of 1 % (w/v) NaCl. The phylogenetic trees based on 16S rRNA gene sequences revealed that strain CAU 9143 belonged to the genus Arthrobacter and was closely related to Arthrobacter ginkgonis SYP-A7299 (97.1 % similarity). Strain CAU 9143 contained menaquinone MK-9 (H2) as the major respiratory quinone and diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, two glycolipids and two unidentified phospholipids as the major polar lipids. The whole-cell sugars were glucose and galactose. The peptidoglycan type was A4a (l-Lys-D-Glu2) and the major cellular fatty acid was anteiso-C15 : 0. The DNA G+C content was 64.4 mol% and the level of DNA-DNA relatedness between CAU 9143 and the most closely related strain, A. ginkgonis SYP-A7299, was 22.3 %. Based on phenotypic, chemotaxonomic and genetic data, strain CAU 9143 represents a novel species of the genus Arthrobacter, for which the name Arthrobacterpaludis sp. nov. is proposed. The type strain is CAU 9143 (=KCTC 13958,=CECT 8917).
Topics: Arthrobacter; Bacterial Typing Techniques; Base Composition; Cell Wall; DNA, Bacterial; Fatty Acids; Glycolipids; Nucleic Acid Hybridization; Peptidoglycan; Phospholipids; Phylogeny; RNA, Ribosomal, 16S; Republic of Korea; Sequence Analysis, DNA; Vitamin K 2; Wetlands
PubMed: 29099354
DOI: 10.1099/ijsem.0.002426 -
Chemosphere Nov 2023Di(2-ethylhexyl) phthalate (DEHP) has been widely detected in soil, water, and sediment as a priority control pollutant. Immobilized microorganism technology is...
The mechanism of DEHP degradation by the combined action of biochar and Arthrobacter sp. JQ-1: Mechanisms insight from bacteria viability, degradation efficiency and changes in extracellular environment.
Di(2-ethylhexyl) phthalate (DEHP) has been widely detected in soil, water, and sediment as a priority control pollutant. Immobilized microorganism technology is gradually mature and applied in production. Biochar prepared from agricultural wastes is an excellent immobilized carrier because of its porous structure and abundant functional groups. Environmental acidification was caused by degrading bacteria Arthrobacter sp. JQ-1 (JQ-1) respiration and acidic metabolites during DEHP degradation, which affected the passage life of microorganisms and the removal efficiency of DEHP. The mechanism of DEHP degradation by the combined action of JQ-1 and corn straw biochar (BC) at 600 °C was investigated, and bacterial viability, microenvironmental changes, and kinetic tests were performed in this research. Compared with biodegradation group alone, the degradation rate of DEHP in 1% biochar unloaded and loaded with JQ-1 increased by 18.3% and 30.9%, and its half-life decreased to 23.90 h and 11.95h, a reduction of 31.37 h. The percentage of detected living JQ-1 increased as biochar content increased when loading capacity was less than 1%. In which, (JQ-1-BC2) group was 4.1% higher than (JQ-1-BC1) group. Biochar has the ability to neutralize acidifying environmental pH due to its alkaline functional groups, including lactone group, -OH, -COO-. 1% biochar loaded with JQ-1 increased the pH of the microenvironment by 0.57 and alkaline phosphatase (AKP) activity by 0.0063 U·mL, which promoted the reduction of PA. Study suggested that biochar loaded with JQ-1 could simultaneously adsorb and degrade DEHP during the process of DEHP removal. Biochar could be used as a biological stimulant to increase abundance and metabolism, enhance the utilization of DEHP by JQ-1. Biochar (1% (w/v)) loaded with JQ-1 as DEHP removal material showed good performance. Biochar not only as an immobilized carrier, but also as a biostimulant, providing an effective strategy for the collaborative remediation of PAEs contaminated.
Topics: Diethylhexyl Phthalate; Arthrobacter; Microbial Viability; Soil Pollutants; Biodegradation, Environmental; Soil; Phthalic Acids
PubMed: 37678595
DOI: 10.1016/j.chemosphere.2023.140093