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International Journal of Molecular... Mar 2020Saliva plays a crucial role in oral cavity. In addition to its buffering and moisturizing properties, saliva fulfills many biofunctional requirements, including...
Saliva plays a crucial role in oral cavity. In addition to its buffering and moisturizing properties, saliva fulfills many biofunctional requirements, including antibacterial activity that is essential to assure proper oral microbiota growth. Due to numerous extra- and intra-systemic factors, there are many disorders of its secretion, leading to oral dryness. Saliva substitutes used in such situations must meet many demands. This study was design to evaluate the effect of core-shell magnetic nanoparticles (MNPs) adding (gold-coated and aminosilane-coated nanoparticles NPs) on antimicrobial (microorganism adhesion, biofilm formation), rheological (viscosity, viscoelasticity) and physicochemical (pH, surface tension, conductivity) properties of three commercially available saliva formulations. Upon the addition of NPs (20 µg/mL), antibacterial activity of artificial saliva was found to increase against tested microorganisms by 20% to 50%. NPs, especially gold-coated ones, decrease the adhesion of Gram-positive and fungal cells by 65% and Gram-negative bacteria cells by 45%. Moreover, the addition of NPs strengthened the antimicrobial properties of tested artificial saliva, without influencing their rheological and physicochemical properties, which stay within the range characterizing the natural saliva collected from healthy subjects.
Topics: Anti-Infective Agents; Bacterial Adhesion; Biofilms; Candida; Elasticity; Electric Conductivity; Gold; Magnetite Nanoparticles; Pseudomonas; Saliva, Artificial; Silanes; Streptococcus; Surface Tension; Viscosity
PubMed: 32183193
DOI: 10.3390/ijms21061979 -
Supportive Care in Cancer : Official... Mar 2015Dry mouth (xerostomia) is one of the commonest symptoms in cancer patients and can adversely affect quality of life. The aim of this review was to determine the... (Review)
Review
PURPOSE
Dry mouth (xerostomia) is one of the commonest symptoms in cancer patients and can adversely affect quality of life. The aim of this review was to determine the effectiveness of pharmacological and non-pharmacological interventions in treating xerostomia in adult advanced cancer patients.
METHODS
The literature search was performed in February 2014 using databases including EMBASE, MEDLINE, CINAHL, BNI and Cochrane library. The search was carried out using standard MeSH terms and was limited to adult population and English language. Studies investigating xerostomia secondary to head and neck cancer treatment and autoimmune disease were excluded. Titles and abstracts were screened and reviewed for eligibility. Only studies involving primary research were included in the analysis.
RESULTS
Six studies met the eligibility criteria for review: three randomized controlled trials and three prospective studies. The quality assessment and reporting was performed using PRISMA, Jadad and STROBE. These studies compared acupuncture, pilocarpine, Saliva Orthana and chewing gum with each other or with placebo. All interventions were considered effective in treating xerostomia. However, effectiveness versus placebo could not be demonstrated for Saliva Orthana. Meta-analysis could not be performed due to heterogeneity of the study type and intervention.
CONCLUSION
Limited published data exists reporting the effectiveness of measures in the treatment of xerostomia in cancer patients. Based on primary research of low quality, firm conclusions cannot be drawn. However, pilocarpine, artificial saliva, chewing gum and acupuncture can be tried based on the available data. This highlights the explicit need to improve our evidence base. Properly constructed randomized controlled trials demonstrating effectiveness of pharmacological and non-pharmacological interventions for dry mouth are required.
Topics: Acupuncture Therapy; Adult; Chewing Gum; Disease Progression; Humans; Neoplasms; Pilocarpine; Prospective Studies; Quality of Life; Saliva, Artificial; Xerostomia
PubMed: 25322971
DOI: 10.1007/s00520-014-2477-8 -
Caries Research 2017The effect of mucin in artificial saliva on rehardening (RE-experiment) and inhibition (DE-experiment) of erosion was evaluated. The treatment groups were: artificial... (Comparative Study)
Comparative Study
The effect of mucin in artificial saliva on rehardening (RE-experiment) and inhibition (DE-experiment) of erosion was evaluated. The treatment groups were: artificial saliva with mucin, artificial saliva without mucin, human saliva, and water. For the RE-experiment, after immersion of enamel blocks in citric acid (4 min), hardness was measured and blocks were subjected to treatment for 2 h. For the DE-experiment, sound blocks were subjected to treatment for 2 h and immersed in citric acid (4 min). Percentages of hardness recovery (RE) and loss (DE) were analyzed (ANOVA/Tukey's test). The salivas promoted similar rehardening, but only the saliva with mucin was similar to human saliva with regard to enamel protection against erosion.
Topics: Animals; Cattle; Hardness; Humans; Mucins; Saliva; Saliva, Artificial; Tooth; Tooth Demineralization; Tooth Erosion
PubMed: 28122369
DOI: 10.1159/000454817 -
Special Care in Dentistry : Official... May 2019To evaluate the efficacy of saliva substitutes and stimulants compared to placebo in improving findings of dry mouth not caused by Sjӧgren's. (Review)
Review
AIMS
To evaluate the efficacy of saliva substitutes and stimulants compared to placebo in improving findings of dry mouth not caused by Sjӧgren's.
METHODS AND RESULTS
Four databases were searched (PubMed, Web of Science, Cochrane Library, and EMBASE) through March 2018. Only randomized controlled trials (RCT) using placebo interventions with dry mouth patients were included. Outcomes included salivary flow and self-reported patient improvement. The Cochrane Collaboration's tool for assessing risk of bias was used. A total of 348 references were reviewed, resulting in 7 RCTs with 257 dry mouth patients with 5 studies at high risk of bias, 1 unclear and 1 low risk being included. Topical spray of 1% malic acid increased unstimulated (Difference in Means [DM] = 0.096, 95% CI = 0.092-0.100; P < 0.001) and stimulated (DM = 0.203, 95% CI = 0.106-0.300; P < 0.001) salivary flow in 2 studies. Patients using 1% malic acid topical spray were 5.4 times more likely to report improvement compared to placebo (95% CI = 2.634-11.091; P < 0.001) in 2 studies. No significant improvements were found with other products when compared to placebo.
CONCLUSION
Use of 1% malic acid topical spray seems to improve dry mouth compared to placebo. Quality of the evidence was low due to risk of bias and imprecision.
Topics: Humans; Randomized Controlled Trials as Topic; Saliva; Saliva, Artificial; Xerostomia
PubMed: 30811076
DOI: 10.1111/scd.12370 -
Food Chemistry Jul 2023Astringency is one of the most important organoleptic characteristics of red wines, and its intensity evaluation method has been the focus of research in recent years....
Astringency is one of the most important organoleptic characteristics of red wines, and its intensity evaluation method has been the focus of research in recent years. An artificial saliva system was developed to establish an accurate and reliable evaluation method for the astringency intensity of dry red wines based on saliva precipitation index (SPI). To achieve this, five key protein families, which presented high reactivities and sensitivities in protein-tannin binding reactions, were selected from human whole saliva. The concentrations of the five proteins (proline-rich protein, α-amylase, lactoferrin, lysozyme, and albumin) and pH were optimized using response surface methodology based on the human salivary conditions to simulate the real salivary environment. The artificial saliva precipitation index method was applied to 60 commercial dry red wines and it exhibited a high correlation (Coef = 0.94) with the sensory scores, indicating better performance than the traditional SPI method and other analytical approaches.
Topics: Humans; Astringents; Wine; Saliva, Artificial; Salivary Proteins and Peptides; Tannins; Saliva; Taste
PubMed: 36750006
DOI: 10.1016/j.foodchem.2023.135628 -
Caries Research 2016The aim of this study was to evaluate the erosion-preventive effect of different artificial saliva formulations and human saliva in vitro compared to human saliva in...
The aim of this study was to evaluate the erosion-preventive effect of different artificial saliva formulations and human saliva in vitro compared to human saliva in situ. In the in vitro experiment, bovine enamel and dentin specimens were stored in artificial saliva (4 different formulations, each n = 20), deionized water (n = 20) or human saliva (n = 6 enamel and dentin specimens/volunteer) for 120 min. In the in situ experiment, each of the 6 enamel and dentin specimens was worn intraorally by 10 volunteers for 120 min. The specimens were then eroded (HCl, pH 2.6, 60 s). Half of the specimens were subjected to microhardness analysis (enamel) and the determination of calcium release into the acid (enamel and dentin), while the other half were again placed in the respective medium or worn intraorally, respectively, for 120 min before a second erosion was performed. Knoop microhardness of enamel and the calcium release of enamel and dentin into the acid were again determined. Statistical analysis was conducted by two-way repeated-measures ANOVA or two-way ANOVA (α = 0.05). Enamel microhardness was not significantly different between all test groups after the first and the second erosive challenge, respectively. Enamel calcium loss was significantly lower in situ compared to the in vitro experiment, where there was no significant difference between all test groups. Dentin calcium loss was significantly lower than deionized water only after the first and than all except one artificial saliva after the second erosion. Under the conditions of this experiment, the use of artificial saliva formulations and human saliva in vitro does not reflect the intraoral situation in dental erosion experiments adequately.
Topics: Animals; Cattle; Dental Enamel; Dentin; Saliva; Saliva, Artificial; Tooth Erosion
PubMed: 26870948
DOI: 10.1159/000443188 -
Applied and Environmental Microbiology Mar 2023The outbreak of SARS-CoV-2 has emphasized the need for a deeper understanding of infectivity, spread, and treatment of airborne viruses. Bacteriophages (phages) serve as...
The outbreak of SARS-CoV-2 has emphasized the need for a deeper understanding of infectivity, spread, and treatment of airborne viruses. Bacteriophages (phages) serve as ideal surrogates for respiratory pathogenic viruses thanks to their high tractability and the structural similarities tailless phages bear to viral pathogens. However, the aerosolization of enveloped SARS-CoV-2 surrogate phi6 usually results in a >3-log reduction in viability, limiting its usefulness as a surrogate for aerosolized coronavirus in "real world" contexts, such as a sneeze or cough. Recent work has shown that saliva or artificial saliva greatly improves the stability of viruses in aerosols and microdroplets relative to standard dilution/storage buffers like suspension medium (SM) buffer. These findings led us to investigate whether we could formulate media that preserves the viability of phi6 and other phages in artificially derived aerosols. Results indicate that SM buffer supplemented with bovine serum albumin (BSA) significantly improves the recovery of airborne phi6, MS2, and 80α and outperforms commercially formulated artificial saliva. Particle sizing and acoustic particle trapping data indicate that BSA supplementation dose-dependently improves viral survivability by reducing the extent of particle evaporation. These data suggest that our viral preservation medium may facilitate a lower-cost alternative to artificial saliva for future applied aerobiology studies. We have identified common and inexpensive lab reagents that confer increased aerosol survivability on phi6 and other phages. Our results suggest that soluble protein is a key protective component in nebulizing medium. Protein supplementation likely reduces exposure of the phage to the air-water interface by reducing the extent of particle evaporation. These findings will be useful for applications in which researchers wish to improve the survivability of these (and likely other) aerosolized viruses to better approximate highly transmissible airborne viruses like SARS-CoV-2.
Topics: Humans; Saliva, Artificial; SARS-CoV-2; COVID-19; Respiratory Aerosols and Droplets; Viruses; Bacteriophages
PubMed: 36856430
DOI: 10.1128/aem.01545-22 -
Acta Odontologica Latinoamericana : AOL Apr 2023Albumin is a salivary enzyme capable of cleaving ester linkages and catalyzing degradation of resin-based dental materials. However, the effect of...
UNLABELLED
Albumin is a salivary enzyme capable of cleaving ester linkages and catalyzing degradation of resin-based dental materials. However, the effect of concentration-dependent esterolytic action on composite resins as yet remains unexplored.
AIM
The purpose of this study was to evaluate whether artificial saliva formulations with different concentrations of albumin affected the surface roughness, flexural strength and microhardness of a composite resin.
MATERIALS AND METHOD
Specimens (25x2x2mm) of a nanofilled composite (Filtek Z350XT, 3M/ESPE) were prepared and analyzed for average surface roughness (Ra/pm). The specimens were then allocated to 6 groups (n=30), to be treated with different salivary albumin concentrations: 0, 10, 50, 100, 200, 400 pg/mL. The specimens were stored in their respective artificial saliva groups, half of them for 24 h and the remainder for 180 days (artificial saliva renewed weekly), after which they were submitted to a new Ra reading, and tested for three-point flexural strength (FS, MPa). The specimens stored for 180 days were analyzed for Knoop microhardness (KH, Kg/mm). Data were submitted to two-way ANOVA (Ra and FS) and one-way ANOVA (KH).
RESULTS
Although Ra increased (p < 0.001) and FS decreased (p < 0.001) from 24 hours to 180 days of storage, the albumin concentration did not significantly affectRa (p = 0.168), FS (p = 0.477) or KH (p = 0.378).
CONCLUSION
The esterolytic action of albumin did not increase the artificial-saliva-induced hydrolytic degradation of the composite resin.
Topics: Composite Resins; Saliva, Artificial; Albumins; Esters
PubMed: 37315309
DOI: 10.54589/aol.36/1/34 -
Chemical and mechanical stability of an ion-exchanged lithium disilicate glass in artificial saliva.Journal of the Mechanical Behavior of... Jan 2023Multi-component lithium disilicate (LD) glasses were ion-exchanged in a pure or mixed nitrate salt bath. The surface morphologies, mechanical properties, chemical...
Multi-component lithium disilicate (LD) glasses were ion-exchanged in a pure or mixed nitrate salt bath. The surface morphologies, mechanical properties, chemical stability and ion leaching of ion-exchanged LD glasses before and after storage in artificial saliva for 21 days were investigated. It can be found that chemical stability of ion-exchanged LD glass was temperature-dependent. The residual compressive stress induced by ion-exchange increased the chemical potential of alkali ions in glass, and the ion-exchanged LD glass, especially 235 °C/64 h group, chemical stability in artificial saliva for 21 days were deteriorated. Back-exchange treatment could relax the stress on the outermost layer of the ion-exchanged LD glass without deteriorating its strengthening effect, and back-exchanged LD glass presented good chemical and mechanical stability in artificial saliva. The results might help to enhance the service stability of ion-exchanged LD glass-ceramics in the oral condition.
Topics: Saliva, Artificial; Materials Testing; Glass; Dental Porcelain
PubMed: 36375276
DOI: 10.1016/j.jmbbm.2022.105563 -
Scientific Reports Oct 2016During dental erosion, tooth minerals are dissolved, leading to a softening of the surface and consequently to irreversible surface loss. Components from human saliva...
During dental erosion, tooth minerals are dissolved, leading to a softening of the surface and consequently to irreversible surface loss. Components from human saliva form a pellicle on the tooth surface, providing some protection against erosion. To assess the effect of different components and compositions of saliva on the protective potential of the pellicle against enamel erosion, we prepared four different kinds of saliva: human whole stimulated saliva (HS), artificial saliva containing only ions (AS), human saliva dialysed against artificial saliva, containing salivary proteins and ions (HS/AS), and human saliva dialysed against deionised water, containing only salivary proteins but no ions (HS/DW). Enamel specimens underwent four cycles of immersion in either HS, AS, HS/AS, HS/DW, or a humid chamber (Ctrl), followed by erosion with citric acid. During the cycling process, the surface hardness and the calcium released from the surface of the specimens were measured. The different kinds of saliva provided different levels of protection, HS/DW exhibiting significantly better protection than all the other groups (p < 0.0001). Different components of saliva, therefore, have different effects on the protective properties of the pellicle and the right proportions of these components in saliva are critical for the ability to form a protective pellicle.
Topics: Adult; Dialysis; Humans; Ions; Saliva; Salivary Proteins and Peptides; Tooth Erosion; Young Adult
PubMed: 27703230
DOI: 10.1038/srep34760