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Applied Biochemistry and Biotechnology Jan 2023Astaxanthin and β-carotene are the most prominent carotenoids extensively used in pharmaceutics. Here, we present a halotolerant bacterium from Lake Wadi El-Natrun...
Astaxanthin and β-carotene are the most prominent carotenoids extensively used in pharmaceutics. Here, we present a halotolerant bacterium from Lake Wadi El-Natrun capable of producing astaxanthin and β-carotene analyzed by HPLC, ESI-MS, and infrared spectroscopy. The phenotypic and phylogenetic analyses classified the isolate as a novel strain of the genus Planococcus, for which the name Planococcus sp. Eg-Natrun is proposed. Carotenoid biosynthesis can exceptionally occur in a light-inducible or constitutive manner. The maximum carotenoid yields were 610 ± 13 µg/g (~ 38% β-carotene and ~ 21% astaxanthin) in a minimal medium with acetate and 1024 ± 53 µg/g dry cells in a rich marine medium. The carotenogenesis incentives (e.g., acetate) and disincentives (e.g., methomyl) were discussed. Moreover, we successfully isolated the CrtE gene, one of the astaxanthin biosynthesis genes, from the unknown genome using a consensus-based degenerate PCR approach. To our knowledge, this is the first report elucidating astaxanthin and β-carotene in the genus Planococcus.
Topics: beta Carotene; Phylogeny; Xanthophylls; Carotenoids
PubMed: 36070166
DOI: 10.1007/s12010-022-04148-4 -
Journal of Agricultural and Food... Sep 2022Astaxanthin (AST), a fat-soluble carotenoid, shows excellent antioxidant and anti-inflammatory activities, but its low biocompatibility and stability limit its...
Astaxanthin (AST), a fat-soluble carotenoid, shows excellent antioxidant and anti-inflammatory activities, but its low biocompatibility and stability limit its application in the food industry. In this work, we constructed the targeted hyaluronic acid (HA)-modified milk exosome-based astaxanthin delivery system to improve the biocompatibility stability and targeted transport properties of astaxanthin. Nuclear magnetic resonance (NMR) and Fourier transform infrared spectroscopy (FTIR) showed that HA was efficiently modified onto the surface of the milk exosome by an amide condensation reaction. The fluorescence images showed that the targeted delivery system accumulated in RAW264.7 macrophages, and the targeting effect on inflammatory cells was significantly enhanced. Compared with free astaxanthin, the delivery system could enhance the cellular uptake of astaxanthin and alleviate the overproduction of reactive oxygen species significantly and the depolarization of mitochondrial membrane potential in a lipopolysaccharide-induced cellular model. The delivery system also notably inhibited the expression of IL-1β, IL-6, and other inflammatory factors. Therefore, the targeted hyaluronic acid-modified milk exosome-based astaxanthin delivery system prevents the activation of macrophages and the production of inflammatory mediators and has the potential to apply to the prevention of chronic inflammatory diseases.
Topics: Animals; Exosomes; Hyaluronic Acid; Milk; Xanthophylls
PubMed: 36018242
DOI: 10.1021/acs.jafc.2c03683 -
Bioresource Technology Nov 2023Microalgal astaxanthin possesses numerous bioactivities and has several health applications. The current research focuses on designing and optimizing the two-stage...
Microalgal astaxanthin possesses numerous bioactivities and has several health applications. The current research focuses on designing and optimizing the two-stage mixotrophic bioprocess by Chlorella zofingiensis for astaxanthin production. Gradual increase in light intensity (4-8k-lux) and 3x micronutrient concentration were the key parameters for maximizing biomass yield of 2.5 g/L during 15 days of stage I. Furthermore, stress conditions (excessive CO light, salinity, etc.) enhanced astaxanthin yield at stage II. 20k lux light, 3x nutrients, and 5% CO were the best ranges for maximum astaxanthin production. Maximum biomass yield and astaxanthin content were 3.3 g/L and 16.7 mg/g, respectively, after 29 days of bioprocess. Astaxanthin biosynthesis was also affected by salinity, but less than other parameters. Astaxanthin bioprocess resulted in enhanced lipid yields of 35-37%, which could be used for biodiesel. This study shows promising scale-up potential with attractive sustainability features of Chlorella zofingiensis model for commercial astaxanthin-lipid biorefinery.
Topics: Microalgae; Chlorella; Xanthophylls; Biomass; Lipids
PubMed: 37544537
DOI: 10.1016/j.biortech.2023.129635 -
Marine Drugs Aug 2023The use of conventional astaxanthin extraction methods, typically involving organic solvents, leads to a heightened environmental impact. The aim of this study was to...
BACKGROUND
The use of conventional astaxanthin extraction methods, typically involving organic solvents, leads to a heightened environmental impact. The aim of this study was to explore the potential use of environmentally friendly extraction solvents, such as vegetable oils, for recovering the shrimp by-product astaxanthin.
METHODS
Ultrasound-assisted extraction (UAE) in vegetable oils, including olive oil (OO), sunflower oil (SO), and flaxseed oil (FO), was employed to extract astaxanthin. The astaxanthin antioxidant activity was evaluated using an ABTS assay, and a mixture of gum Arabic and soy lecithin was used to form coacervates to produce astaxanthin encapsulation.
RESULTS
A by-product-vegetable oil ratio of 1:60, extraction time of 210 min, 60% amplitude of the extraction process, and the use of OO as the extracting medium resulted in an astaxanthin yield of 235 ± 4.07 μg astaxanthin/g by-products. The astaxanthin encapsulation efficiency on day 0 and astaxanthin recovery on day 1 were recorded at 66.6 ± 2.7% and 94.4 ± 4.6%, respectively.
CONCLUSIONS
The utilization of OO as an extraction solvent for astaxanthin from shrimp by-products in UAE represents a novel and promising approach to reducing the environmental impact of shrimp by-products. The effective astaxanthin encapsulation efficiency highlights its potential application in food industries.
Topics: Animals; Plant Oils; Ultrasonics; Xanthophylls; Crustacea; Solvents
PubMed: 37755080
DOI: 10.3390/md21090467 -
Photosynthesis Research Sep 2015Massive accumulation of the secondary ketokarotenoid astaxanthin is a characteristic stress response of certain microalgal species with Haematococcus pluvialis as an... (Review)
Review
Massive accumulation of the secondary ketokarotenoid astaxanthin is a characteristic stress response of certain microalgal species with Haematococcus pluvialis as an illustrious example. The carotenogenic response confers these organisms a remarkable ability to survive in extremely unfavorable environments and makes them the richest source of natural astaxanthin. Exerting a plethora of beneficial effects on human and animal health, astaxanthin is among the most important bioproducts from microalgae. Though our understanding of astaxanthin biosynthesis, induction, and regulation is far from complete, this gap is filling rapidly with new knowledge generated predominantly by application of advanced "omics" approaches. This review focuses on the most recent progress in the biology of astaxanthin accumulation in microalgae including the genomic, proteomic, and metabolomics insights into the induction and regulation of secondary carotenogenesis and its role in stress tolerance of the photosynthetic microorganisms. Special attention is paid to the coupling of the carotenoid and lipid biosynthesis as well as deposition of astaxanthin in the algal cell. The place of the carotenogenic response among the stress tolerance mechanisms is revisited, and possible implications of the new findings for biotechnological production of astaxanthin from microalgae are considered. The potential use of the carotenogenic microalgae as a source not only of value-added carotenoids, but also of biofuel precursors is discussed.
Topics: Microalgae; Xanthophylls
PubMed: 25975708
DOI: 10.1007/s11120-015-0156-3 -
Advances in Experimental Medicine and... 2021Paracoccus carotinifaciens is an aerobic Gram-negative bacterium that exhibits motility by a peritrichous flagellum. It produces a carotenoid mixture containing...
Paracoccus carotinifaciens is an aerobic Gram-negative bacterium that exhibits motility by a peritrichous flagellum. It produces a carotenoid mixture containing astaxanthin as the main component. Selective breeding of P. carotinifaciens has been performed using classical techniques for mutation induction, such as chemical treatment and ultraviolet irradiation, and not using genetic engineering technology. The commercial production of astaxanthin with P. carotinifaciens has been established by optimizing fermentation medium and conditions in the process. Dehydrated P. carotinifaciens is used as a coloring agent for farmed fish and egg yolks. Compared with the administration of chemically synthesized astaxanthin, dehydrated P. carotinifaciens imparts more natural coloration, which is favored by consumers. In addition, astaxanthin-rich carotenoid extracts (ARE) derived from P. carotinifaciens are developed for human nutrition. Animal and clinical studies with ARE for evaluating its efficacy have been conducted and suggested that ARE would be useful for preventing anxiety, stomach ulcer, and retinal damage, as well as improving cognitive function. The efficacy is anticipated to result from not only astaxanthin but also other carotenoids in ARE, such as adonirubin and adonixanthin, in some studies. Hence, astaxanthin commercially produced with P. carotinifaciens has been applied widely in animals and humans.
Topics: Animals; Anxiety; Humans; Paracoccus; Xanthophylls
PubMed: 33783727
DOI: 10.1007/978-981-15-7360-6_2 -
Marine Drugs Sep 2020Several antitumour drugs have been isolated from natural products and many clinical trials are underway to evaluate their potential. There have been numerous reports...
Several antitumour drugs have been isolated from natural products and many clinical trials are underway to evaluate their potential. There have been numerous reports about the antitumour effects of astaxanthin against several tumours but no studies into its effects against glioblastoma. Astaxanthin is a red pigment found in crustaceans and fish and is also synthesized in ; adonixanthin is an intermediate product of astaxanthin. It is known that both astaxanthin and adonixanthin possess radical scavenging activity and can confer a protective effect on several damages. In this study, we clarified the antitumour effects of astaxanthin and adonixanthin using glioblastoma models. Specifically, astaxanthin and adonixanthin showed an ability to suppress cell proliferation and migration in three types of glioblastoma cells. Furthermore, these compounds were confirmed to transfer to the brain in a murine model. In the murine orthotopic glioblastoma model, glioblastoma progression was suppressed by the oral administration of astaxanthin and adonixanthin at 10 and 30 mg/kg, respectively, for 10 days. These results suggest that both astaxanthin and adonixanthin have potential as treatments for glioblastoma.
Topics: Administration, Oral; Animals; Antineoplastic Agents; Brain Neoplasms; Carotenoids; Cell Line, Tumor; Disease Progression; Glioblastoma; Humans; Male; Mice; Mice, Inbred C57BL; Mice, Inbred ICR; Xanthophylls
PubMed: 32962073
DOI: 10.3390/md18090474 -
Biomaterials Jan 2023Smart delivery systems with stimuli-responsive capability are able to improve the bioaccessibility through increasing the solubility, physicochemical stability and...
Smart delivery systems with stimuli-responsive capability are able to improve the bioaccessibility through increasing the solubility, physicochemical stability and biocompatibility of bioactive compounds. In this study, the astaxanthin nanoparticles with reactive oxygen species (ROS) and pH dual-response function were design and constructed using poly (propylene sulfide) covalently modified sodium alginate as carriers based on ultrasonic assisted self-assembly strategy. Atomic force microscope and scanning electron microscope analysis showed that the nanoparticles were spherical in shape with a size of around 260 nm. Meanwhile, the astaxanthin nanoparticles showed both pH and ROS stimuli-responsive release characteristics. In vitro cell experiments showed that astaxanthin nanoparticles significantly inhibited the production of ROS and mitochondrial depolarization induced by oxidative stress. In vivo colitis experiment of mice revealed that astaxanthin nanoparticles could significantly relieve colitis, protect the integrity of colon tissue and restore the expression of tight junction proteins ZO-1 and occludin. The abundance of Lactobacillus and Lachnospiraceae, and the ratio of Firmicutes/Bacteroidota of gut microbiota were significantly improved after intervention of the stimuli-responsive astaxanthin nanoparticles. This work provided a simple strategy for constructing ROS/pH dual response delivery system, which provided an experimental basis for improving the oral bioavailability of hydrophobic active compounds.
Topics: Mice; Animals; Reactive Oxygen Species; Nanoparticles; Colitis; Hydrogen-Ion Concentration
PubMed: 36495803
DOI: 10.1016/j.biomaterials.2022.121937 -
Journal of the Science of Food and... Aug 2022Oil bodies (OBs) are a kind of natural and stable oil nucleate microcapsule in which the triglyceride matrix can be used as an appropriate carrier of hydrophobic...
BACKGROUND
Oil bodies (OBs) are a kind of natural and stable oil nucleate microcapsule in which the triglyceride matrix can be used as an appropriate carrier of hydrophobic molecules. Astaxanthin has high antioxidant properties but is extremely sensitive to oxidation, causing the loss of its bioactive properties.
RESULTS
The purpose of this study was to clarify the effects of environmental factors (light, oxygen, temperature, and pH) on the physical and oxidative stability of astaxanthin microcapsules prepared with peanut oil bodies (POBs). After 14 days of storage, the retention rate of astaxanthin in peanut oil microcapsules (POMs) was significantly increased. The astaxanthin retention rate of POMs stored under light conditions was higher than under dark conditions. Similarly, the retention rate of astaxanthin in POMs was significantly increased during vacuum storage. The astaxanthin retention rate was also the highest when POMs were stored at 4 °C, whereas it was the lowest at pH 3.0.
CONCLUSION
The experiment demonstrated that microcapsulation could improve the astaxanthin retention rate and storage stability, and recombinant OBs were potential ideal wall materials for astaxanthin embedding. © 2022 Society of Chemical Industry.
Topics: Capsules; Liposomes; Oxidative Stress; Xanthophylls
PubMed: 35246844
DOI: 10.1002/jsfa.11854 -
Critical Reviews in Food Science and... 2023Microorganisms such as bacteria, microalgae and fungi, are natural and rich sources of several valuable bioactive antioxidant's compounds, including carotenoids. Among... (Review)
Review
Microorganisms such as bacteria, microalgae and fungi, are natural and rich sources of several valuable bioactive antioxidant's compounds, including carotenoids. Among the carotenoids with antioxidant properties, astaxanthin can be highlighted due to its pharmaceutical, feed, food, cosmetic and biotechnological applications. The best-known producers of astaxanthin are yeast and microalgae cells that biosynthesize this pigment intracellularly, requiring efficient and sustainable downstream procedures for its recovery. Conventional multi-step procedures usually involve the consumption of large amounts of volatile organic compounds (VOCs), which are regarded as toxic and hazardous chemicals. Considering these environmental issues, this review is focused on revealing the potential of unconventional extraction procedures [., Supercritical Fluid Extraction (SFE), Ultrasound-Assisted Extraction (UAE), Microwave-Assisted Extraction (MAE), High-Pressure Homogenization (HPH)] combined with alternative green solvents (biosolvents, eutectic solvents and ionic liquids) for the recovery of microbial-based astaxanthin from microalgae (such as ) and yeast (such as ) cells. The principal advances in the area, process bottlenecks, solvent selection and strategies to improve the recovery of microbial astaxanthin are emphasized. The promising recovery yields using these environmentally friendly procedures in lab-scale are good indications and directions for their effective use in biotechnological processes for the production of commercial feed and food ingredients like astaxanthin.
Topics: Antioxidants; Biomass; Saccharomyces cerevisiae; Carotenoids; Solvents; Microalgae
PubMed: 35766952
DOI: 10.1080/10408398.2022.2093326