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Environment International Jan 2023Pesticide exposure has been associated with adverse health effects. We evaluated relationships between proximity to agricultural insecticide applications and...
BACKGROUND
Pesticide exposure has been associated with adverse health effects. We evaluated relationships between proximity to agricultural insecticide applications and insecticides in household dust, accounting for land use and wind direction.
METHODS
We measured concentrations (ng/g) of nine insecticides in carpet-dust samples collected from 598 California homes. Using a geographic information system (GIS), we integrated the California Pesticide Use Reporting (CPUR) database to estimate agricultural use within residential buffers with radii of 0.5 to 4 km. We calculated the density of use (kg/km) during 30-, 60-, 180-, and 365-day periods prior to dust collection and evaluated relationships between three density metrics (CPUR unit-based, agricultural land area adjusted, and average daily wind direction adjusted) and dust concentrations. We modeled natural-log transformed concentrations using Tobit regression for carbaryl, chlorpyrifos, cypermethrin, diazinon, and permethrin. Odds of detection were modeled with logistic regression for azinphos-methyl, cyfluthrin, malathion, and phosmet. We adjusted for season, year, occupation, and home/garden uses.
RESULTS
Chlorpyrifos use within 1-4 km was associated with 1 to 2-times higher dust concentrations in both the 60- and 365-day periods. Carbaryl applications within 2-4 km of homes 60-days prior to dust collection were associated with 3 to 7-times higher concentrations and the 4 km trend was strongest using the wind-adjusted metric (p-trend = 0.04). For diazinon, there were 2-times higher concentrations for the 60-day metrics in the 2 km buffer and for the CPUR and wind-adjusted metrics within 4 km. Cyfluthrin, phosmet, and azinphos-methyl applications within 4 km in the prior 365-days were associated with 2-, 6-, and 3-fold higher odds of detection, respectively.
CONCLUSIONS
Agricultural use of six of the nine insecticides within 4 km is an important determinant of indoor contamination. Our findings demonstrated that GIS-based metrics for quantifying potential exposure to fugitive emissions from agriculture should incorporate tailored distances and time periods and support wind-adjustment for some, but not all insecticides.
Topics: Insecticides; Chlorpyrifos; Diazinon; Azinphosmethyl; Environmental Exposure; Phosmet; Carbaryl; Agriculture; Pesticides; Dust
PubMed: 36493610
DOI: 10.1016/j.envint.2022.107657 -
Journal of Applied Toxicology : JAT Jan 2019Organophosphorus compounds (OPCs), inhibitors of acetylcholinesterase (AChE), are useful agents as pesticides, but also represent a serious health hazard. Standard... (Review)
Review
Organophosphorus compounds (OPCs), inhibitors of acetylcholinesterase (AChE), are useful agents as pesticides, but also represent a serious health hazard. Standard therapy with atropine and established oxime-type enzyme reactivators (pralidoxime, obidoxime) is unsatisfactory. Better therapeutic results are obtained, when reversible AChE inhibitors are administered before OPC exposure. This review summarizes the history of such a pretreatment approach and sums up a set of experiments undertaken in search of compounds that are efficacious when given before a broad range of OPCs. The prophylactic efficacy of 10 known AChE inhibitors, either already used clinically for different indications (physostigmine, pyridostigmine, ranitidine, tiapride, tacrine, amiloride, metoclopramide, methylene blue) or developed for possible therapeutic use in the future (7-methoxytacrine, K-27) was compared, when administered before exposure to six chemically diverse OPCs in the same experimental setting: ethyl-paraoxon, methyl-paraoxon, diisopropylfluorophosphate, terbufos sulfone, azinphos-methyl and dicrotophos. The experimental oxime K-27 was the most efficacious compound, affording best protection, when administered before terbufos sulfone, azinphos-methyl and dicrotophos, second best before ethyl- and methyl-paraoxon exposure and third best before diisopropylfluorophosphate administration. This ranking was similar to that of physostigmine, which was superior to the Food and Drug Administration-approved pretreatment for soman with pyridostigmine. Tiapride, amiloride, metoclopramide, methylene blue and 7-methoxytacrine did not achieve protection. No correlation was observed between the IC of the reversible AChE inhibitors and their protective efficacy. These studies indicate that K-27 can be considered a very promising broad-spectrum prophylactic agent in case of imminent organophosphate exposure, which may be related to its AChE reactivating activity rather than its AChE inhibition.
Topics: Animals; Cholinesterase Inhibitors; Humans; Models, Animal; Organophosphate Poisoning; Organophosphates; Pre-Exposure Prophylaxis
PubMed: 30027640
DOI: 10.1002/jat.3662 -
Environmental Analysis, Health and... Dec 2022The aim of the study was to assess the occurrence and distribution of organophosphate compounds residue in soil, surface water, sediment, and banana crops in Araromi...
The aim of the study was to assess the occurrence and distribution of organophosphate compounds residue in soil, surface water, sediment, and banana crops in Araromi farm settlement, Osun State, Nigeria. Organophosphate pesticide residues were determined using a gas chromatography equipped with Flame-Ionization Detection (GC-FID) in 16 soil samples from cocoa and banana farms, 6 water and sediment samples each, and 8 banana samples from 4 farms in the study site. Fourteen organophosphate compounds were detected (acephate, omethoate, dementon-s-methyl, dimethoate, tolcofos-methyl, pirimiphos-methyl, malathion, chlorpyrifos, methidathion, prothiofos, profenofos, ethion, azinphos-methyl and pyrazophos). Tolclofos-methyl, pirimiphos-methyl and prothiofos were detected in all the soil and sediment samples with concentration ranges of 1.9-12.9, 2.25-6.98 and 3.38-9.89 mg/kg respectively in soil and 8.13-9.83, 2.82-25.1 and 3.70-19.5 mg/kg respectively in sediment. Dimethoate, pirimiphos-methyl and prothiofos with concentration ranges, 0.06-0.28, 0.09-0.18 and 0.16-6.11 mg/L respectively were mostly detected in water samples while dimethoate, tolcofos-methyl, malathion, methidathion, prothiofos, ethion and azinphos-methyl compounds were detected in all the banana samples with concentration ranges, 3.40-12.0, 1.82-6.26, 5.73-9.48, 29.7-145, 8.24-20.1, 3.87-9.35 and 3.66-12.2 mg/kg respectively. The organophosphate mean residue concentrations were mostly significantly higher than the Maximum Residue Limits (MRL) at p<0.05. Across the three samples, only pirimiphos-methyl was significantly higher in water samples, omethoate in sediment; acephate, dementon-s-methyl and chlorpyrifos in banana were also not significantly higher at p<0.05. A strong positive significant correlation was observed between the organophosphate compounds in the banana and water samples (R=0.77, p=0.002) at p<0.05. The occurrence of organophosphate compounds in concentrations above MRLs may pose serious environmental and health risks.
PubMed: 36916048
DOI: 10.5620/eaht.2022035 -
The Science of the Total Environment Dec 2017Beeswax from Spain was collected during 2016 to determine pesticide residues incidence. The 35 samples were divided in foundation, old combs, cappings or virgin beeswax...
Beeswax from Spain was collected during 2016 to determine pesticide residues incidence. The 35 samples were divided in foundation, old combs, cappings or virgin beeswax to compare pesticide content between groups. Wax was screened for 58 pesticides or their degradation products by QuEChERS extraction and liquid chromatography mass spectrometry (LC-MS/MS). Beeswax was uniformly contaminated with acaricides and, to a much lesser extent, with insecticide and fungicide residues. Virgin followed by cappings were less contaminated than foundation and old combs beeswax. The miticides applied in-hive had a contribution to average pesticide load higher than 95%. Compounds widely used as acaricides, as coumaphos (100%), fluvalinate (86%) and amitraz (83%), were the pesticides most frequently detected with maximum concentrations of 26,858, 3593 and 6884ng·g, respectively. Chlorfenvinphos, acrinathrin and flumethrin, also acaricides, were detected in 77, 71 and 54%, respectively. Frequencies of pesticides used in crops were 40% for chlorpyrifos, 29% for dichlofenthion, 9% for malathion, 6% for fenthion-sulfoxide and 3% for azinphos-methyl, carbendazim, ethion, hexythiazox, imazalil and pyriproxyfen. Pesticide assessment in beeswax could be an excellent monitoring tool to establish veterinary treatments applied by beekeepers and environmental contaminants exposure of honey bees.
Topics: Animals; Bees; Pesticide Residues; Spain; Waxes
PubMed: 28679118
DOI: 10.1016/j.scitotenv.2017.06.174 -
Journal of Applied Toxicology : JAT Nov 2022One of many noteworthy consequences of increasing societal reliance on pesticides is their predominance in aquatic environments. These pernicious chemicals interact with...
Interactive effects of high temperature and pesticide exposure on oxidative status, apoptosis, and renin expression in kidney of goldfish: Molecular and cellular mechanisms of widespread kidney damage and renin attenuation.
One of many noteworthy consequences of increasing societal reliance on pesticides is their predominance in aquatic environments. These pernicious chemicals interact with high temperatures from global climate change, heat waves, and natural variations to create unstable environments that negatively impact organisms' health. To understand these conditions, we examined the dose-dependent effects of environmentally relevant pesticide mixtures (metolachlor, linuron, isoproturon, tebuconazole, aclonifen, atrazine, pendimethalin, and azinphos-methyl) combined with elevated temperatures (22 control vs. 32°C for 4-week exposure) on renin, dinitrophenyl protein (DNP, an indicator of reactive oxygen species, ROS), 3-nitrotyrosine protein (NTP, an indicator of reactive nitrogen species, RNS), superoxidase dismutase (SOD, an antioxidant), and catalase (CAT, an antioxidant) expressions in the kidneys of goldfish (Carassius auratus). Histopathological analysis showed widespread damage to kidney tissues in high temperature and pesticide co-exposure groups, including rupture of the epithelial layer, hemorrhaging, and degeneration of tubular epithelium. Quantitative real-time polymerase chain reaction (qRT-PCR) and immunohistochemical analyses demonstrated significant declines in renin receptor-like mRNA and protein expressions in kidney tissues under combined exposure to high temperature and pesticides compared with controls; conversely, expression of DNP, NTP, SOD, and CAT increased in kidney tissues under the same conditions. Apoptotic cells were also increased in co-exposure groups as assessed by in situ terminal deoxynucleotidyl transferase dUTP nick labeling (TUNEL) assay. The enhanced apoptosis in kidneys of heat and pesticides co-exposed fish was associated with increased caspase-3 (a protease enzyme) mRNA levels. Our results demonstrated that high temperature and pesticides induced oxidative/nitrative stress (i.e., ROS/RNS), damaged tissues, increased cellular apoptosis, and suppressed renin expression in kidneys of goldfish.
Topics: Animals; Antioxidants; Apoptosis; Atrazine; Azinphosmethyl; Caspase 3; Catalase; DNA Nucleotidylexotransferase; Goldfish; Hot Temperature; Kidney; Linuron; Oxidative Stress; Pesticides; RNA, Messenger; Reactive Nitrogen Species; Reactive Oxygen Species; Renin; Superoxide Dismutase; Temperature
PubMed: 35698815
DOI: 10.1002/jat.4357 -
Marine Pollution Bulletin Jul 2023Micropollutants such as pesticides and the prediction of water quality in aquatic environments have been known as a serious risk to the environment and human health. The...
Micropollutants such as pesticides and the prediction of water quality in aquatic environments have been known as a serious risk to the environment and human health. The pollution level of six pesticides-three organochlorines (OCPs: aldrin, dieldrin, and endrin) and three organophosphates (OPPs: diazinon, malathion, and azinphosmethyl)- in water, sediment, and fish samples was examined in the Miankaleh wetland, Iran. Water quality, including dissolved oxygen (DO), biological oxygen demand (BOD), chemical oxygen demand (COD), salinity, electrical conductivity (EC), turbidity, total dissolved solids (TDS), pH, temperature, and physicochemical properties of sediments, was analyzed. Low concentrations of OCPs (0.70 ± 0.01 μg/L) and OPPs (1.31 ± 0.1 μg/L) were observed in water. In contrast, OCPs and OPPs were not detected in sediment and fish samples in the Miankaleh wetland. Low concentrations of OCPs and OPPs in water and no pesticide concentrations in sediment and fish samples indicate low contamination of the aquatic environment in Miankaleh. The results of this study could be used as an effective reference for policy makers in the field of water resource management.
Topics: Animals; Humans; Wetlands; Iran; Water Pollutants, Chemical; Environmental Monitoring; Pesticides; Hydrocarbons, Chlorinated; Insecticides; Organophosphates; Geologic Sediments
PubMed: 37267870
DOI: 10.1016/j.marpolbul.2023.115097 -
Journal of Toxicology and Environmental... Mar 2023The synergy between multiple compounds and other stressors, including heat, creates volatility and greater unpredictability than standard single-chemical toxicity...
The synergy between multiple compounds and other stressors, including heat, creates volatility and greater unpredictability than standard single-chemical toxicity testing, especially in the case of pesticides and metabolites which might contain several noxious ingredients resulting in adverse ecological effects. To address this, the aim of this study was to examine the dose- and time-dependent effects of low- and high-dose pesticide mixture (metalachlor, linuron, isoproturon, tebucanazole, aclonifen, atrazine, pendimethalin, azinphos-methyl) and heat stress co-exposure (22°C control/32°C treatment for 4-week) on free-swimming behaviors and cumulative actionless time (CAT) of goldfish. Behavioral analysis showed a dose- and time-dependent decrease in distance swam, as well as a subsequent increase in CAT. Vertical and horizontal spatial behavioral use were affected under heat and pesticides co-exposure conditions. In 3- and 4-week(s) exposure groups, horizontal spatial behavioral use demonstrated elevated time spent in the lower third of the aquarium. Similarly, during 3- and 4-week(s) exposure (32°C control and 32°C high doses) vertical spatial behavioral use was found to increase time spent in the outermost edges of the aquarium. In all treatment groups, the final condition factor (KM) showed significant attenuation when compared to the initial KM. However, there was an unclear relationship between heat/pesticide co-exposure and growth most notably in 32°C high-dose groups. In addition, the expression of hepatic cytochrome P450 1A mRNA was significantly higher in pesticide-exposed groups. Taken together, data demonstrated that co-exposure with low- or high-dose pesticide mixture and heat stress significantly impacted natural swimming patterns, which over time might result in the broader population and ecological effects.
Topics: Animals; Pesticides; Goldfish; Swimming; Temperature; Cytochrome P-450 Enzyme System
PubMed: 36756740
DOI: 10.1080/15287394.2023.2174463 -
Foods (Basel, Switzerland) Jun 2023Pesticides are extensively used in the cultivation and postharvest protection of citrus fruits, therefore continuous monitoring and health risk assessments of their...
Pesticides are extensively used in the cultivation and postharvest protection of citrus fruits, therefore continuous monitoring and health risk assessments of their residues are required. This study aimed to investigate the occurrence of pesticide residues on citrus fruits and to evaluate the acute and chronic risk for adults and children. The risk ranking of twenty-three detected pesticides was carried out according to a matrix ranking scheme. Multiple residues were detected in 83% of 76 analyzed samples. In addition, 28% contained pesticides at or above maximum residue levels (MRLs). The most frequently detected pesticides were imazalil, azoxystrobin, and dimethomorph. According to the risk ranking method, imazalil was classified in the high-risk group, followed by prochloraz, chlorpyrifos, azinphos-methyl, tebufenpyrad, and fenpiroximate, which were considered to pose a medium risk. The majority of detected pesticides (74%) posed a low risk. The health risk assessment indicated that imazalil and thiabendazole contribute to acute (HQa) and chronic (HQc) dietary risk, respectively. The HQc was negligible for the general population, while the HQa of imazalil and thiabendazole exceeded the acceptable level in the worst-case scenario. Cumulative chronic/acute risk (HIc/HIa) assessment showed that chronic risk was acceptable in all samples for children and adults, while the acute risk was unacceptable in 5.3% of citrus fruits for adults and 26% of citrus fruits for children. Sensitivity analyses indicated that the ingestion rate and individual body weight were the most influential risk factors.
PubMed: 37444192
DOI: 10.3390/foods12132454 -
International Journal of Hygiene and... Mar 2023Our prior work shows that azinphos-methyl pesticide exposure is associated with altered oral microbiomes in exposed farmworkers. Here we extend this analysis to show the...
Our prior work shows that azinphos-methyl pesticide exposure is associated with altered oral microbiomes in exposed farmworkers. Here we extend this analysis to show the same association pattern is also evident in their children. Oral buccal swab samples were analyzed at two time points, the apple thinning season in spring-summer 2005 for 78 children and 101 adults and the non-spray season in winter 2006 for 62 children and 82 adults. The pesticide exposure for the children were defined by the farmworker occupation of the cohabitating household adult and the blood azinphos-methyl detection of the cohabitating adult. Oral buccal swab 16S rRNA sequencing determined taxonomic microbiota proportional composition from concurrent samples from both adults and children. Analysis of the identified bacteria showed significant proportional changes for 12 of 23 common oral microbiome genera in association with azinphos-methyl detection and farmworker occupation. The most common significantly altered genera had reductions in the abundance of Streptococcus, suggesting an anti-microbial effect of the pesticide. Principal component analysis of the microbiome identified two primary clusters, with association of principal component 1 to azinphos-methyl blood detection and farmworker occupational status of the household. The children's buccal microbiota composition clustered with their household adult in ∼95% of the households. Household adult farmworker occupation and household pesticide exposure is associated with significant alterations in their children's oral microbiome composition. This suggests that parental occupational exposure and pesticide take-home exposure pathways elicit alteration of their children's microbiomes.
Topics: Adult; Humans; Child; Pesticides; Farmers; Azinphosmethyl; RNA, Ribosomal, 16S; Agriculture; Occupational Exposure; Microbiota
PubMed: 36516690
DOI: 10.1016/j.ijheh.2022.114090 -
Environmental Science and Pollution... Apr 2018Agriculture represents the second most important economic activity in the North Patagonian Region of Argentina and non-selective insecticides are still being used with...
Agriculture represents the second most important economic activity in the North Patagonian Region of Argentina and non-selective insecticides are still being used with significant implications to the quality of the environment. The range of concentrations (μg/L) determined for azinphosmethyl, chlorpyrifos, and carbaryl in drainage channels were from non-detected to 1.02, 1.45, and 11.21, respectively. Macroinvertebrate abundance and taxon richness in drainage channels were significantly lower in November compared to the other sampling months (October, February). The decrease in taxon richness observed in November was associated with chlorpyrifos and azinphosmethyl peak concentrations. The most remarkable changes were the decrease in sensitive taxa such as Baetidae and the increase in some tolerant taxa such as Chironomidae and Gastropoda.For all three pesticides, the acute hazard quotient exceeded the risk criteria for invertebrates. The effects of the three pesticides on aquatic organisms, characterized by joint probability curves, showed that the LC of 10% of the species were exceeded five and three times by the concentrations of azinphosmethyl and chlorpyrifos during the study period, respectively. However, the correlation between the pesticide concentrations and both taxon richness and abundance of macroinvertebrates at each site (irrigation and drainage channels) was indicative that only chlorpyrifos was negatively correlated with both parameters (Spearman r - 0.61, p = 0.0051 and Spearman r - 0.59, p = 0.0068 for taxon richness and abundance correlation, respectively). We conclude that macroinvertebrate assemblages in drainage channels were highly affected by chlorpyrifos levels.
Topics: Agriculture; Animals; Aquaporins; Argentina; Chlorpyrifos; Gastropoda; Insecticides; Pesticides; Water
PubMed: 29392605
DOI: 10.1007/s11356-018-1330-x