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Journal of Pharmaceutical and... Nov 2018A novel HPLC method for the determination of the impurities in desonide cream was established and validated for the further improvement of the official monograph in USP....
A novel HPLC method for the determination of the impurities in desonide cream was established and validated for the further improvement of the official monograph in USP. Desonide was well resolved from the photodegradation impurity, which overlapped with desonide in USP method. The method was validated in accordance to the regulatory guidelines recommended by the International Conference on Harmonisation and this validation included specificity, limit of detection, limit of quantification, linearity and accuracy. Four degradation impurities in desonide cream were characterized by a trap-free two-dimensional liquid chromatography coupled to high resolution ion trap/time-of-flight mass spectrometry (2D LC-IT-TOF MS) in positive mode of electrospray ionization. Through the multiple heart-cutting 2D LC approach and online demineralization technique, the problem of incompatibility between non-volatile salt mobile phase and mass spectrometry was solved completely, and the TIC chromatogram of LC-MS could be in conformity with the LC chromatogram of the official analytical method in the peak sequence of impurities. In the first dimension, the column was Phenomenex Kinetex C8 (4.6 mm × 150 mm, 2.6 μm) with a non-volatile salt mobile phase. In the second dimension, the column was Shimadzu Shim-pack GISS C18 (50 mm × 2.1 mm, 1.9 μm) with a volatile salt mobile phase. The structures of four degradation impurities in desonide cream were deduced based on the HPLC-MS data. The established method in this study was simple and reliable for routine quality control of desonide cream.
Topics: Chromatography, High Pressure Liquid; Chromatography, Liquid; Desonide; Drug Contamination; Skin Cream; Spectrometry, Mass, Electrospray Ionization
PubMed: 30205304
DOI: 10.1016/j.jpba.2018.08.055 -
Pakistan Journal of Pharmaceutical... May 2019To observe and analyze the clinical efficacy of mucopolysaccharide polysulfate (MPS) ointment combined with desonide ointment in treatment of infantile eczema. A total... (Randomized Controlled Trial)
Randomized Controlled Trial
To observe and analyze the clinical efficacy of mucopolysaccharide polysulfate (MPS) ointment combined with desonide ointment in treatment of infantile eczema. A total of 180 infants who had been treated for eczema at our hospital were enrolled. The patients were divided into control group accepting desonide ointment only and research group accepting mucopolysaccharide polysulfate ointment and desonide ointment. The therapeutic efficacies of two groups were compared. Results: By comparing the total therapeutic efficacy, results showed that the total efficacy of the research group was 96.67%, while that value of the control group was 82.22%, making the total efficacy of the research group significantly higher (p<0.05). And the improvement of the Eczema Area and Severity Index (EASI) score in the research group after drug administration was significantly better than that of the control group (p<0.05). Moreover, there was a greater decrease in the recurrence rate of the research group than that of the control group (p<0.05). Combined application of mucopolysaccharide polysulfate ointment and desonide ointment can achieve better therapeutic effect in treatment of infantile eczema.
Topics: Anti-Inflammatory Agents; Child, Preschool; Dermatitis, Atopic; Desonide; Drug Therapy, Combination; Female; Glycosaminoglycans; Humans; Infant; Male; Ointments; Treatment Outcome
PubMed: 31551225
DOI: No ID Found -
Pharmaceutical Development and... Jul 2022Nanotechnological products have been used as strategies to optimize the therapy and minimize the side effects of topical corticoids. The objective of this study was to...
Nanotechnological products have been used as strategies to optimize the therapy and minimize the side effects of topical corticoids. The objective of this study was to develop hydrogels by the addition of sclerotium gum to the suspensions of desonide-loaded açai oil-based nanocapsules and to study their biological effect using an animal model of acute skin inflammation. The hydrogels presented a pH compatible with topical application (4.4 to 5.0), nanometric mean diameter (131 to 165 nm), pseudoplastic behavior, and stability under room conditions during 30 days. The skin permeation/penetration study demonstrated that a higher amount of desonide ( < 0.05) was retained in the epidermis from the nanotechnological-hydrogels (0.33 to 0.36 µg.cm) in comparison to the commercial gel cream (0.16 µg.cm). In the dermis, the nanostructured hydrogels promoted a lower DES retention compared to the non-nanostructured formulations ( < 0.05). This result may indicate a smaller amount of drug reaching the bloodstream and, thus, fewer side effects can be expected. Concerning the anti-inflammatory effect, the developed hydrogels reduced both ear edema and inflammatory cell infiltration, showing an effect comparable to the commercially available formulation, which presents twice the drug concentration. The hydrogels developed may be considered a promising approach to treat dermatological disorders.
Topics: Animals; Anti-Inflammatory Agents; Desonide; Glucocorticoids; Hydrogels; Nanocapsules
PubMed: 35850635
DOI: 10.1080/10837450.2022.2103147 -
Skinmed 2021A 40-year-old man presented with pruritic perianal lesions existing for 18 months, accompanied by frequent burning sensation on defecating. He had received oral...
A 40-year-old man presented with pruritic perianal lesions existing for 18 months, accompanied by frequent burning sensation on defecating. He had received oral fluconazole 150 mg weekly for a presumptive diagnosis of candidosis earlier. There was no history of other mucocutaneous lesions or comorbidities. The patient denied any unprotected sexual exposure or having anal intercourse. Examination revealed a well-defined perianal, horseshoe-shaped, white-colored macerated plaque with fissures on the gluteal cleft (Figure 1) with tenderness. Examination of scalp, nail, and oral mucosa was unremarkable. Routine investigations, including complete blood counts and chemical profile, were within normal limits. His fasting glucose level was 85 mg/ dL and glycated hemoglobin (HbA1c) was 5.3. The Venereal Disease Research Laboratory (VDRL) test and testing for human immunodeficiency virus (HIV)-1 and 2 were nonreactive. A 10% potassium hydroxide (KOH) examination was negative for fungal elements. Differential diagnoses of flexural psoriasis, Hailey-Hailey disease, and lichen sclerosus were considered, and a skin biopsy for pathology and direct immunofluorescence was performed. The pathologic features showed epidermal acanthosis, hyperkeratosis with elongated rete ridges, and focal hypogranulosis. The upper part of the dermis showed a moderate perivascular mononuclear inflammatory infiltrate with a few melanophages (Figure 2). Direct immunofluorescence was negative. Based on pathologic features, a diagnosis of psoriasis alba was confirmed, and the patient was started on desonide lotion (0.05%) to be applied in the morning and tacrolimus cream (0.1%) at night, plus oral antihistamines. At the follow-up visit 3 weeks later, the lesions diminished to a great extent (Figure 3).
Topics: Adult; Biopsy; Diagnosis, Differential; Humans; Male; Psoriasis
PubMed: 34861923
DOI: No ID Found -
Clinical and Molecular Allergy : CMA Nov 2022Food allergy is becoming increasingly common among the pediatric population. Despite strict avoidance of food allergens, a subgroup of sensitive individuals still...
BACKGROUND
Food allergy is becoming increasingly common among the pediatric population. Despite strict avoidance of food allergens, a subgroup of sensitive individuals still develops frequent, persistent, and difficult to treat hives (FPDTH) for which there is no curative therapy. Although these cases are rare, these patients are in most need of therapy.
CASE PRESENTATIONS
This is a retrospective review of 3 pediatric patients with highly sensitive food allergies who initially presented with hives daily or every other day, or multiple times a day, but achieved marked remission after traditional Chinese medicine (TCM) therapies. Patient 1 (P1) is a 5-year-old who has experienced 140 reactions in his lifetime. Reactions were mostly hives with 4 episodes of anaphylaxis. P1 had used Prednisone 20 times, had an Epinephrine injection 4 times, and had 3 emergency room (ER) visits. Patient 2 (P2) is a 12-year-old who had experienced hives since age 3. Despite daily antihistamine use, P2 had > 730 reactions in his lifetime at the time of presentation including 2 episodes of anaphylaxis. He had been prescribed prednisone 4 times, an Epinephrine injection 2 times, and had 1 ER visit. Patient 3 (P3) is a 20-month-old girl who had experienced > 120 reactions including 1 episode of anaphylaxis. She was on daily desonide and frequently used an antihistamine, yet still had required a course of prednisone once, an Epinephrine injection once, and had 1 ER visit to manage her reaction. After presenting to our clinic, patients received internal and external TCM treatments, including herbal baths and creams (Remedy A-D) as basic remedies to reduce food reactions, including but not limited to frequent hives. Within 7-9 months of TCM treatment, remarkably all patients had complete remission of atopic symptoms. All three patients also experienced an improvement in other conditions including food intolerance, diarrhea, anxiety, eczema, and environmental allergies. After 1 year of treatment, all three patients had reductions in food-specific IgE levels that had been previously elevated, and additionally, P1 and P3, who initially had high total IgE levels, experienced a marked decrease in total IgE levels as well. All three patients continued to introduce foods into their diet that they previously had reactions to, and all 3 patients remain symptom-free.
CONCLUSIONS
Three pediatric patients with a known history of multiple food sensitivities and physician-diagnosed food allergies that presented with FPDTH underwent a TCM regimen and experienced dramatic improvement in symptoms and reduction in their IgE levels. This regimen appears to be effective in FPDTH population although a further study in a controlled clinical setting is required.
PubMed: 36434719
DOI: 10.1186/s12948-022-00175-y -
Journal of Cosmetic Dermatology May 2024The current nursing procedure after fractional carbon dioxide (fCO) is complex and needs to be optimized. The present study was conducted to evaluate the assisting... (Randomized Controlled Trial)
Randomized Controlled Trial
BACKGROUND
The current nursing procedure after fractional carbon dioxide (fCO) is complex and needs to be optimized. The present study was conducted to evaluate the assisting effect of filament coating system after fCO laser treatment.
METHODS
Chinese individuals aged from 18 to 65 years diagnosed as photoaging or atrophic acne scar were recruited and each participant was treated with one single pass of fCO laser. A split face was randomly assigned as treatment side or control side. For control side, conventional procedure was topically applied respectively, including desonide cream two times for 3 days, fusidic acid cream two times for 7 days, and recombinant human epidermal growth factor (RhEGF) gel four times for 7 days; for treating side, a filament coating system was applied immediately after one application of fusidic acid cream, desonide cream and RhEGF, and removed 3 h later, for 3 days. Erythema, edema, crust, and pain on both sides were scored from 0 to 10 before and 1, 2, 4, and 7 days after fCO laser treatment. Stratum corneum hydration (SCH) and sebum of forehead and cheek on both sides were also measured by using Corneometer-Sebumeter.
RESULTS
Twenty photoaging and 11 atrophic acne scar participants finished the observation. All of them complained of erythema, edema, crust, and pain after fCO laser treatment, and the scores decreased as time passed by. There were no statistical significances of erythema, edema, crust, pain, SCH, and sebum between treating side and control side at each observation time.
CONCLUSION
Filament coating system was effective, safe, convenient, and economic in assisting recovery of ablative fCO laser, which might be a new option for additional nursing procedure.
Topics: Humans; Adult; Middle Aged; Female; Lasers, Gas; Acne Vulgaris; Male; Young Adult; Skin Aging; Adolescent; Cicatrix; Aged; Treatment Outcome
PubMed: 38192154
DOI: 10.1111/jocd.16169 -
The Journal of Family Practice Jan 2017Patch testing data indicate that the 5 most prevalent contact allergens out of more than 3700 that are known are: nickel (14.3% of patients tested), fragrance mix (14%),...
Patch testing data indicate that the 5 most prevalent contact allergens out of more than 3700 that are known are: nickel (14.3% of patients tested), fragrance mix (14%), the topical antibiotic neomycin (11.6%), balsam of Peru (used in some perfumes, toiletries, and pharmaceutical items) (10.4%), and the mercury-based vaccine preservative thimerosal (10.4%).
Topics: Allergens; Child; Dermatitis, Allergic Contact; Desonide; Exanthema; Eyebrows; Female; Humans; Nickel; Treatment Outcome; Umbilicus
PubMed: 28188316
DOI: No ID Found -
Journal of Photochemistry and... Oct 2019This study aimed to develop Eudragit® RL 100 nanocapsules loaded with desonide (DES) using açai oil (AO) or medium chain triglycerides (MCT) as oil core....
This study aimed to develop Eudragit® RL 100 nanocapsules loaded with desonide (DES) using açai oil (AO) or medium chain triglycerides (MCT) as oil core. Pre-formulation study showed that AO and MCT are suitable for nanocapsules preparation. The nanocapsules prepared with AO and MCT presented mean particle size around 165 and 131 nm, respectively; polydispersity index values <0.20, positive zeta potential values, drug content close to the theoretical value (0.25 mg mL), and DES encapsulation efficiency around 81%, regardless of the oil core (AO or MCT). Considering the photoinstability reported to DES, photodegradation studies were performed. The UV-A (365 nm) and UV-C (254 nm) photodegradation studies revealed less DES degradation when associated to the nanocapsules containing AO in comparison to those with MCT. The in vitro release study showed a biphasic release profile for both nanocapsule suspensions: an initial burst effect followed by a prolonged DES release. In addition, the formulations were considered non-phototoxic at 0.5 mg mL when tested on 3 T3 murine fibroblasts and HaCaT human keratinocytes using the MTT and NRU viability assays. The irritant potential of the prepared nanocapsules and DES in free form were evaluated by HET-CAM method. All formulations were classified as slightly irritant, including the non-associate DES. In conclusion, the nanocapsule formulations developed in this study may be promising for therapeutic applications.
Topics: Animals; Anti-Inflammatory Agents; Cell Line; Cell Survival; Desonide; Drug Compounding; Drug Liberation; Drug Stability; Euterpe; Humans; Light; Mice; Nanocapsules; Particle Size; Photolysis; Plant Oils; Polymethacrylic Acids; Suspensions; Triglycerides
PubMed: 31522112
DOI: 10.1016/j.jphotobiol.2019.111606 -
BioMed Research International 2022A topological index is a real number derived from the structure of a chemical graph. It is helpful to determine the physicochemical and biological properties of a wide...
A topological index is a real number derived from the structure of a chemical graph. It is helpful to determine the physicochemical and biological properties of a wide range of drugs, and it better reflects the theoretical properties of organic compounds. This is accomplished using degree-based topological indices. Vitiligo is a common, acquired skin pigmentation disorder that significantly impacts the quality of life. It frequently embodies a therapeutic challenge, resulting in interest in alternative treatments based on vitamin and herbal supplements. In this article, azathioprine, clobetasol, desonide, hydrocortisone valerate, and other drugs utilized to cure vitiligo have discoursed, and the goal of QSPR revision is to determine the mathematical relationship between properties under investigation (e.g., polarity and enthalpy) and diverse descriptors associated with the drugs' molecule. The QSPR model will help to predict physical properties. In this study, topological indices (TIs) imposed on said drugs were found to have a good correlation with physicochemical properties in this course. Finally, this work can be helpful to design and synthesize new vitiligo treatments and other disease drugs.
Topics: Humans; Vitiligo; Quantitative Structure-Activity Relationship; Quality of Life; Autoimmune Diseases; Restraint, Physical
PubMed: 36425334
DOI: 10.1155/2022/6045066 -
Se Pu = Chinese Journal of... Dec 2023Glucocorticoids, which are a class of steroidal hormones secreted by the adrenal cortex, have significant anti-inflammatory, immunosuppressive, and anti-allergic...
Glucocorticoids, which are a class of steroidal hormones secreted by the adrenal cortex, have significant anti-inflammatory, immunosuppressive, and anti-allergic effects. Thus, these compounds are widely used in clinical practice. However, the long-term use of cosmetics containing glucocorticoids can lead to serious consequences, such as hormone-dependent dermatitis, hypertension, and other serious injuries. The Safety and Technical Specification for Cosmetics (2015 edition) and Regulation (EC) No. 1223/2009 of the European Parliament and Council on cosmetic products list glucocorticoids as prohibited raw materials. According to the National Medical Products Administration, reports on the illegal addition of glucocorticoids to cosmetics by manufacturers have increased in recent years. Therefore, establishing high-throughput screening methods to ensure the quality and safety of cosmetics is imperative. In this study, a comprehensive analytical method based on ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) was developed for the rapid screening of 83 glucocorticoids in cosmetics. A series of conditions were optimized using three matrices that are commonly used in cosmetics: water, lotion, and cream (o/w-type). Four mobile-phase systems and three chromatographic columns were then optimized to achieve the best separation effects. Various MS parameters, such as the capillary voltages, cone voltages, desolvation gas flow rates, and collision energies of the ion pairs of the target compounds, were also optimized. Furthermore, pretreatment was essential for glucocorticoid determination owing to the complex matrix effects of cosmetics. The analytes were divided into two groups, with lg =4 as the limit, to compare the effects of the extraction solvent on recoveries. The extraction recoveries of target analytes with six extraction methods, namely, extraction with acetonitrile, extraction with acetone, extraction with ethyl acetate, dispersion in saturated sodium chloride solution followed by extraction with acetonitrile, dispersion in saturated sodium chloride solution followed by extraction with acetone, and dispersion in saturated sodium chloride solution followed by extraction with ethyl acetate, were compared. The recoveries from QuEChERS and solid-phase extraction (SPE) purification were also compared. Based on the experimental results, the final sample pretreatment method included acetonitrile vortex dispersion, ultrasonic extraction, and sample loading after filtration. The 83 target compounds were separated on a Thermo Accucore PFP column (100 mm×2.1 mm, 2.6 μm) with 0.1% (v/v) acetic acid in acetonitrile and 0.1% (v/v) acetic acid in water as the mobile phases. The analytes were determined by dynamic multiple-reaction monitoring (MRM) in electrospray positive ionization mode (ESI) and quantified using the external standard method. Matrix standard curves were used to reduce matrix effects. The calibration curves of the 83 target compounds were linear in the mass concentration range of 2-200 μg/L (>0.995). At three levels of addition, the recoveries were 74.5%-112.4%, and the relative standard deviations (RSDs, =6) were 0.8%-9.9%. The limits of detection (LODs, ≥3) were 0.001-0.023 μg/g, and the limits of quantification (LOQs, ≥10) were 0.002-0.076 μg/g. The developed method was used to detect glucocorticoids in 41 cosmetic samples. Fluocinolone acetonide, beclomethasone dipropionate, desonide 21-acetate, and desonide were detected in four samples. The content range of glucocorticoids in the positive samples was 0.53-634.27 μg/g. Notably, desonide 21-acetate, which is not included in the scope of the statutory detection method, was detected in two batches of samples. In conclusion, the proposed method is simple, sensitive, reliable, and suitable for the high-throughput analysis of the 83 glucocorticoids in cosmetics with different matrices. This method could provide reliable technical support for the daily supervision of cosmetics and serve as a supplement to current glucocorticoid standards.
Topics: Glucocorticoids; Acetone; Chromatography, High Pressure Liquid; Chromatography, Liquid; Desonide; Sodium Chloride; Tandem Mass Spectrometry; Acetic Acid; Acetonitriles; Water; Cosmetics; Solid Phase Extraction
PubMed: 38093538
DOI: 10.3724/SP.J.1123.2023.04009