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Journal of Ethnopharmacology Aug 2020Dipsacus asper Wall. ex C.B. Clarke, a traditional Chinese herbal medicine, has long been used in China for the therapy of bone diseases (e.g. bone fracture,... (Review)
Review
ETHNOPHARMACOLOGICAL RELEVANCE
Dipsacus asper Wall. ex C.B. Clarke, a traditional Chinese herbal medicine, has long been used in China for the therapy of bone diseases (e.g. bone fracture, osteoporosis, rheumatic arthritis), traumatic hematoma, uterine bleeding and those caused by the deficiency of liver and kidney.
AIM OF THE STUDY
This work aims to evaluate current research progress on chemical constituents, pharmacological activities, quality control, and pharmacokinetic of Dipsacus asper Wall. ex C.B. Clarke, pinpoint the shortcomings of existing studies, and provide meaningful guidelines for our future investigations.
METHODS
Extensive database retrieval, such as PubMed, SciFinder and CNKI, was carried out by using keywords such as "Dipsacus asper", "Radix Dipsaci", and "Xuduan". Furthermore, relevant textbooks, patents, reviews, and digital documents were consulted to collate all available scientific literature and to provide a complete science-based survey of the topic.
RESULTS
More than 100 compounds have been isolated and identified from Dipsacus asper Wall. ex C.B. Clarke, a substantial proportion of which were reported to be triterpenoids and iridoids. Biological effects such as protective effects against bone fracture, anti-osteoporosis, neuroprotective, cardioprotective, anti-aging and protection of reproductive system activities were also evaluated in vitro and in vivo.
CONCLUSIONS
Diaphoretic processing resulted in the drop in the content of asperosaponin VI, which was highly associated with bone protective effect of DA. Therefore, diaphoretic processing was not a suitable processing method for DA. Although Dipsacus asper Wall. ex C.B. Clarke was traditionally used for therapy of osteoarthritis, the in-depth study of the underlying mechanism was very rare. Much endeavor had been made on the effect of DA on bone fracture. Notably, high-dose of Dipsacus asper administration may cause adverse impacts in maternal healthy and embryo-fetal development. It's not suitable for further development in those bioactivities, such as anti-inflammatory and free radical scavenging, which are shared in many other plant species. Pharmacological effects of individual component of DA is not equivalent to its traditional usage. Attention should be paid to the traditional effect of extract of DA.
Topics: Animals; Dipsacaceae; Drugs, Chinese Herbal; Humans; Medicine, Chinese Traditional; Plant Extracts; Quality Control
PubMed: 32348843
DOI: 10.1016/j.jep.2020.112912 -
Nutrition and Cancer 2017In Europe, both the fruits and flowers of Sambucus nigra L. have been used against cold, as well as laxative, diaphoretic, and diuretic remedies. There are also a number...
In Europe, both the fruits and flowers of Sambucus nigra L. have been used against cold, as well as laxative, diaphoretic, and diuretic remedies. There are also a number of commercially available food products that contain elderberry juice, puréed or dried elderberries. Recent comprehensive literature data on pharmacology and chemistry of Sambuci fructus have encouraged us to screen extracts with different polarities from this plant material against cancer cell lines. The cytotoxic activity of the ethyl acetate and aqueous acetone extracts from elderberries as well as detected triterpenoids on human colon adenocarcinoma cell line (LoVo) and human breast cancer cell line (MCF-7) was investigated by sulforhodamine B assay. Moreover, cell migration assay was conducted for triterpenoid fraction and pure compounds. Aqueous acetone extract possessed much lower IC value in cancer cell lines compared to ethyl acetate extract. The latter manifested high cytotoxicity against studied cell lines, suggesting that nonpolar compounds are responsible for the cytotoxic activity. Indeed, the phytochemical analysis revealed that ursolic and oleanolic acids are the main triterpenoids in the mentioned extract of which ursolic acid showed the highest activity with IC values of 10.7 µg/mL on MCF-7 and 7.7 µg/mL on LoVo cells.
Topics: Acetates; Antineoplastic Agents, Phytogenic; Cell Line, Tumor; Cell Movement; Cell Proliferation; Chromatography, High Pressure Liquid; Drug Screening Assays, Antitumor; Humans; Inhibitory Concentration 50; MCF-7 Cells; Oleanolic Acid; Plant Extracts; Sambucus; Triterpenes; Ursolic Acid
PubMed: 28323490
DOI: 10.1080/01635581.2017.1295085 -
Journal of Wound Care Jul 2022The present study aims to create -loaded nanofibre-based wound dressing materials to enhance the wound healing process. is an annual herb native to the Mediterranean...
OBJECTIVE
The present study aims to create -loaded nanofibre-based wound dressing materials to enhance the wound healing process. is an annual herb native to the Mediterranean region. It is antipyretic, antifungal, antioedema, antidiabetic, anti-inflammatory (wound, oral and pharyngeal mucosa), antispasmodic, treats chronic ocular surface diseases, acts as a stimulant and a diaphoretic. It is also used in the prevention of acute dermatitis, and in the treatment of gastrointestinal ulcers, wounds and burns.
METHOD
Electrospinning is an effective method for creating nano- and microfibres for biomedical applications. (CA) of various concentrations 5%, 10% and 15%)-loaded polyvinyl alcohol (PVA)/sodium alginate (SAlg) nanofibre mats were successfully produced via blend electrospinning. Nanofibre mats were evaluated using: scanning electron microscopy (SEM); Fourier transform infrared spectroscopy (FTIR) analysis; gel content; water vapour transmission rate (WVTR); swelling ratio; in vitro drug release studies; viability evaluation (cell culture and MTT assay); and an in vivo study using male Wistar rats. Rats were divided into three groups (n=3). In each group, rats were inflicted with five full-thickness wounds on the back and were treated with sterile gauze (control), PVA/SAlg nanofibre dressing (CA-free control), PVA/SAlg/CA5%, PVA/SAlg/CA10%, and PVA/SAlg/CA15% nanofibre dressing.
RESULTS
Results showed that the obtained fibres were smooth with no surface aggregates, indicating complete incorporation of . The release of from loaded PVA/SAlg fibre mats in the first four hours was burst released and then was constant. PVA/SAlg and PVA/SAlg/CA nanofibres were not toxic to L929 mouse fibroblasts and supported cell attachment and proliferation. The results of the in vivo study showed that the PVA/SAlg/CA10% nanofibre dressing had a higher full-thickness wound healing closure rate compared with the control group on days seven, 14 and 21 after treatment.
CONCLUSION
The results of this evaluation showed that PVA/SAlg/CA nanofibrous mats could be a candidate as an effective wound dressing; however, the percentage of CA in this compound needs further investigation.
Topics: Alginates; Animals; Bandages; Calendula; Male; Mice; Nanofibers; Plant Extracts; Polyvinyl Alcohol; Rats; Rats, Wistar
PubMed: 35797256
DOI: 10.12968/jowc.2022.31.7.598 -
Journal of Ethnopharmacology Oct 2022Inflammation is the body's normal protective response to injury and is stimulated by pathogens, toxic compounds, damaged cells or radiation, promoting healing and...
ETHNOPHARMACOLOGICAL RELEVANCE
Inflammation is the body's normal protective response to injury and is stimulated by pathogens, toxic compounds, damaged cells or radiation, promoting healing and restoring homeostasis to the injured tissue. Leaves of Piper gaudichaudianum Kunth, known as "pariparoba" are widely used in folk medicine for the relief of toothache, while the fresh roots are used as anti-inflammatory and to treat liver disorders. For P. mikanianum (Kunth) Steud is known as "aguaxima", is widely used in the treatment of inflammation, rheumatism and ulcer, with its roots being used for stomach disorders and as a diaphoretic in intermittent fevers.
AIM OF THE STUDY
Therefore, this work aims to chemically characterize the essential oil of Piper gaudichaudianum and Piper mikanianum, as well as the evaluation of neutrophil antichemotactic activity of both essential oils in order to complement the information of its traditional use, taking the leaves as plant material and, with that, corroborating its use in folk medicine for the treatment of inflammatory diseases.
MATERIALS AND METHODS
The essential oil from leaves of both Piper species were obtained from crushed fresh samples, by hydrodistillation using a Clevenger type-apparatus for 4 h. The yield determination was performed as vol/wt (v/w) and in triplicate. The amount of essential oil obtained was quantified in mL. The identification and quantification of the compounds was performed using gas chromatography-mass spectrometry (GC-MS) and gas chromatography-flame ionization detection (GC-FID). The in vitro anti-inflammatory activity was evaluated using the model of modified Boyden chamber. In this test the essential oils were tested for their ability to inhibit leukocyte chemotaxis stimulated by Escherichia coli lipopolysaccharide.
RESULTS
The chemical composition of the essential oils revealed the identification of 26 constituents for P. gaudichaudianum being the sesquiterpenes β-selinene (14.0%) and viridiflorene (10.5%) the main compounds, followed by caryophyllene oxide (9.3%) and (E)-nerolidol (9.0%). For P. mikanianum essential oil, β-myrcene (17.2%) and bicyclogermacrene (26.3%) were the major components in the monoterpenes and sesquiterpene fractions, respectively. The essential oils were also tested for their ability to inhibit neutrophil chemotaxis in vitro when stimulated by Escherichia coli lipopolysaccharide. Both essential oils showed antichemotactic effect with reduction in migration of 0-72.2% for P. gaudichaudianum and 8.6-100% for P. mikanianum to same concentrations, suggesting a response to acute inflammatory processes.
CONCLUSIONS
Since up to date there is no report of this biological activities by this mechanism (antichemotactic assay) for essential oils this species. These results showed that the essential oils of P. gaudichaudianum and P. mikanianum have a great capacity to inhibit neutrophil chemotaxis in an inflammatory process, in a dose-dependent way, suggesting anti-inflammatory potential, by preventing its accumulation at the injury site with the possibility of tissue damage. Findings of these studies support the traditional use of these species in the treatment of inflammatory processes.
Topics: Anti-Inflammatory Agents; Escherichia coli; Gas Chromatography-Mass Spectrometry; Inflammation; Lipopolysaccharides; Oils, Volatile; Piper; Plant Leaves; Plant Oils
PubMed: 35840057
DOI: 10.1016/j.jep.2022.115533 -
PloS One 2018Birch buds (Gemmae Betulae) are widely used in Russian and Chinese traditional medicine mainly as a diuretic and diaphoretic agent but also as an antiseptic,...
Birch buds (Gemmae Betulae) are widely used in Russian and Chinese traditional medicine mainly as a diuretic and diaphoretic agent but also as an antiseptic, anti-inflammatory and analgesic. Despite the long history of therapeutic use of birch buds in folk medicine, the existing information on their chemical composition and pharmacological effects is insufficient. This circumstance warrants further study of the chemistry and pharmacology of birch buds. The present study was designed to investigate (a) the chemical composition of buds from two species of white birch and (b) the in vitro cytotoxic effect of extracts from these sources on selected tumour cells. Extracts from Betula pubescens Ehrh. and Betula pendula Roth. buds were obtained using three different methods: carbon dioxide supercritical fluid extraction (SFE), washing of exudate covering whole buds, and extraction of milled buds with diethyl ether. The chemical composition of extracts was investigated by GC-MS. Cytotoxicity was determined by MTT assay, and cell proliferation was determined by [3H]thymidine uptake in cancer cells and normal skin fibroblasts. The GC-MS investigation identified a total of 150 substances of different classes. The chemical composition of B. pubescens and B. pendula buds differed, with bud extracts from the former containing a relatively high quantity of sesquiterpenoids and flavonoids, while the main components of extracts from the latter were triterpenoids. The results of the biological assay indicated that birch bud extracts demonstrated time- and concentration-dependent and differential cytotoxicity. The highest cytotoxic activity demonstrated bud exudates and SFE extracts obtained from both Betula species. The rich chemical composition of birch buds suggests the possibility of a wider spectrum of biological activity than previously thought. Birch bud extracts could be a promising source of compounds with cytotoxic activity against various cancers.
Topics: Antineoplastic Agents, Phytogenic; Betula; Cell Line, Tumor; Cell Proliferation; Cell Survival; Humans; Inhibitory Concentration 50; Neoplasms; Phytochemicals; Plant Extracts
PubMed: 30106978
DOI: 10.1371/journal.pone.0201949 -
Biological & Pharmaceutical Bulletin Sep 2017Sulfuretin is a natural flavonoid found in the plant Rhus verniciflua STOKES. The plant has been traditionally used as medicinal agent for antiviral, cathartic,...
Sulfuretin is a natural flavonoid found in the plant Rhus verniciflua STOKES. The plant has been traditionally used as medicinal agent for antiviral, cathartic, diaphoretic, anti-rheumatic and sedative activities in East Asia. In this study we isolated and identified sulfuretin from R. verniciflua and investigated its anti-adipogenic activity against 3T3-L1 preadipocytes cells. We evaluated the effects of sulfuretin on the adipogenic transcription factors like peroxisome proliferator-activated receptor γ (PPARγ), CCAAT/enhancer-binding protein α (C/EBPα), fatty acid synthase (FAS), Fabp4, adiponectin and zinc fingerprint protein (Zfp) 521 by gene expression (real-time QPCR) and Western blot analysis. Sulfuretin treatment at Day 0 and 2 showed significant reduction of lipid production in 3T3-L1 cells in concentration dependent manner. Gene expression analysis (real-time PCR) revealed that sulfuretin inhibited the both major adipogenic factors (C/EBPα, C/EBPβ and PPARγ) and minor adipogenic factors (sterol regulatory element-binding protein (SREBP1c), adiponectin, FAS, Fabp4, Zfp423, and Ebf1). Western blot analysis showed the increased expression of β-catenin and suppression of PPARγ after sulfuretin treatment. Overall, sulfuretin is a natural flavonoid having potent anti-adipogenic activity through the suppression of major adipogenic factors C/EBPα, C/EBPβ and PPARγ, which initiate adipogenesis.
Topics: 3T3-L1 Cells; Adipocytes; Adipogenesis; Adipose Tissue; Animals; Anti-Obesity Agents; Benzofurans; CCAAT-Enhancer-Binding Proteins; DNA-Binding Proteins; Flavonoids; Gene Expression; Mice; Obesity; PPAR gamma; Phytotherapy; Plant Extracts; Rhus; Sterol Regulatory Element Binding Protein 1; Transcription Factors; beta Catenin
PubMed: 28579594
DOI: 10.1248/bpb.b17-00049 -
Frontiers in Pharmacology 2023The genus consists of 160 accepted flowering species thriving throughout temperate regions, mainly in the Mediterranean Basin, Northern America, and southwestern and...
The genus consists of 160 accepted flowering species thriving throughout temperate regions, mainly in the Mediterranean Basin, Northern America, and southwestern and eastern Asia. species bear a long-standing record of use in the folk medicine of indigenous tribes and communities worldwide, along with multitudinous applications in traditional cuisines, cosmeceuticals, and agricultural fields. Up-to-date data related to traditional uses, phytochemistry, biological activities, toxicity and clinical trials of the genus were systematically reviewed from several online scientific engines, including PubMed, Web of Science, Scopus, SciFinder, Wiley Online, Science Direct, and Cochrane library. Over the past three decades, 241 metabolites have been isolated from nearly twenty species, including phenolic acids, flavonoids, coumarins, fatty acids and alkanes, aldehydes, volatile compounds, and naphthoquinones. Some unique metabolites have also been identified, such as the ceramides tanacetamide (A-D) from , pyrethrins from , and sesquiterpene lactones from several species. However, these secondary metabolites are still poorly studied despite clues highlighting their colossal pharmacological properties, especially as hypotensive, neuroprotective, anticancer, and antimicrobial agents. Scientific studies have validated some traditional claims of the plant, such as antidiabetic, anticancer, anthelmintic, insecticide, antioxidant, and hepatoprotective activities, as well as against festering wounds, skin ulcers, urinary tract infections, and sexually transmitted diseases. Other ethnomedicinal uses for arthritis, gout, rheumatism, anemia, and as a litholytic, antivenom and diaphoretic have not yet been supported and would constitute the subject of further research.
PubMed: 37153781
DOI: 10.3389/fphar.2023.1169629 -
The Hastings Center Report Mar 2017Dr. Brown, a hospitalist, admits Mark, a patient transferred from a local jail for management of cellulitis. The patient, who was taken into custody two days prior to...
Dr. Brown, a hospitalist, admits Mark, a patient transferred from a local jail for management of cellulitis. The patient, who was taken into custody two days prior to hospital admission, has a history of intravenous heroin use. Mark explains that he had been prescribed buprenorphine-naloxone maintenance therapy for opioid use disorder for several years prior to being arrested and had not used other opioids during that time. As a policy, the jail where Mark is detained does not prescribe opioid agonists, and his maintenance therapy was stopped upon his arrival there. Dr. Brown discovers that Mark is diaphoretic and appears distressed. Mark's symptoms suggest to Dr. Brown that, in addition to having cellulitis, Mark is actively withdrawing from opioids. Mark tells Dr. Brown that he has felt "horrible" since his buprenorphine-naloxone therapy was stopped and that he now has intense cravings for opioids. He asks Dr. Brown to help alleviate the withdrawal symptoms. Dr. Brown, who is accustomed to treating opioid withdrawal with opioid replacement therapy, wonders if she should initiate ORT for Mark while he is in the hospital.
Topics: Hospitalization; Humans; Opiate Substitution Treatment; Opioid-Related Disorders; Patient Discharge; Prisons; Substance Withdrawal Syndrome
PubMed: 28301697
DOI: 10.1002/hast.683 -
Biomedicine & Pharmacotherapy =... Jan 2020Cynaroside (CYN) is the predominant derivative of luteolin in aerial parts of Bidens tripartita which has been used in folk medicine as a diaphoretic, diuretic,...
OBJECTIVES
Cynaroside (CYN) is the predominant derivative of luteolin in aerial parts of Bidens tripartita which has been used in folk medicine as a diaphoretic, diuretic, antiseptic and anti-inflammatory agent. In our study, alginate (ALG), which is an anionic polymer with bioadhesive properties, was used as a CYN carrier, and multiple hydrogel formulations were created. Additionally, the present study evaluated the in vivo anti-inflammatory and anti-allergic activities of all preparations.
METHODS
Novel gel formulations as topical carriers for CYN obtained from B. tripartita were developed and characterized. The bioadhesive properties of the designed preparations were also evaluated in an ex vivo model using the skin of hairless mice. In vitro CYN release from all formulations was examined and analysed by HPLC. Histopathological evaluation of mouse skin sections stained with H&E after carrageenan and oxazolone administration was also carried out. In addition, the influence of CYN on cell proliferation was examined by the PCNA staining method.
RESULTS
The results showed that 10 % CYN inhibited the release of anti-inflammatory mediators, and both tested concentrations, which included 5 % and 10 % (2 mg and 20 mg CYN per site, respectively), reduced oxazolone-induced ear swelling. Histopathological examination of the samples revealed a marked reduction in paw skin and ear tissue inflammation and in inflammatory infiltrates. The influence of CYN on cell proliferation was examined by the PCNA staining method, and the staining and distribution of PCNA-immunoreactive (PCNA-IR) cells were observed. After the application of the 5 % and 10 % hydrogels, the investigated samples showed decreased nuclear immunoreactivity to PCNA, which was similar to that of the control. Moreover, after application of the placebo formulation, fewer PCNA-IR cells were also observed.
CONCLUSION
The obtained data suggest that the topical application of CYN significantly reduces the number of T cells, mast cells and histiocytes in mouse skin with inflammation or atopic dermatitis.
Topics: Animals; Anti-Allergic Agents; Anti-Inflammatory Agents; Disease Models, Animal; Drug Compounding; Drug Liberation; Edema; Glucosides; Hydrogels; Luteolin; Male; Mice, Inbred C57BL; Oxazolone; Proliferating Cell Nuclear Antigen
PubMed: 31810125
DOI: 10.1016/j.biopha.2019.109681 -
Cureus Jun 2022Autoimmune polyendocrine syndrome type 2, also known as Schmidt's syndrome, is a rare autosomal dominant life-threatening syndrome. It is defined by the presence of...
Autoimmune polyendocrine syndrome type 2, also known as Schmidt's syndrome, is a rare autosomal dominant life-threatening syndrome. It is defined by the presence of Addison's disease in combination with at least one of the known autoimmune diseases: thyroid autoimmune disease, type 1 diabetes, and hypogonadism. It is more common in middle-aged females and is treatable if diagnosed early. However, in this case, we report Schmidt's syndrome in a young male without a family history. A 20-year-old male with a past medical history of hypothyroidism, adrenal insufficiency, and type 1 diabetes presented to the emergency department (ED) feeling lethargic, somnolent, and diaphoretic. Laboratory blood tests showed elevated thyroid-stimulating hormone, hyperkalemia of 6.4 mmol/L, and hyponatremia of 131 mmol/l indicating an Addisonian crisis. The patient had low blood glucose (at home: 60 mg/dL, and at ED: 85 mg/dL), hypotensive blood pressure of approximately 85/55 mmHg, and a peaked T-wave on EKG, which were consistent with the diagnosis of Schmidt's syndrome. Based on the laboratory findings and history, the patient was diagnosed with polyendocrine syndrome Type 2 (Schmidt's syndrome). The patient was treated for adrenal insufficiency first followed by thyroid insufficiency. Schmidt's syndrome is a rare disease and difficult to diagnose because the presentation depends on which gland is initially involved. A few cases have been reported in the literature of atypical presentations of Schmidt's syndrome. Therefore, this case report can contribute to the medical literature on Schmidt's syndrome, which can help in early diagnosis and improve patient outcomes.
PubMed: 35911265
DOI: 10.7759/cureus.26322