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Cutaneous and Ocular Toxicology 2015Topical medications are frequently neglected as the potential cause of systemic drug reactions. In this case report, a patient with a maculopapular eruption attributed...
Topical medications are frequently neglected as the potential cause of systemic drug reactions. In this case report, a patient with a maculopapular eruption attributed to a drug hypersensitivity reaction was submitted to skin patch tests in order to clarify the drug implicated. Incidentally, a positive reaction to cinchocaine was observed. With the ulterior confirmation of the application of an antihemorrhoidal ointment containing cinchocaine, which was omitted during the initial anamnesis, the diagnosis of systemic contact dermatitis to cinchocaine was made.
Topics: Dermatitis, Contact; Dibucaine; Female; Humans; Middle Aged; Patch Tests
PubMed: 25198408
DOI: 10.3109/15569527.2014.952375 -
Zhongguo Shi Yan Xue Ye Xue Za Zhi Aug 2015This study was aimed to investigate the regulatory effect of protein disulfide isomerase (PDI) on platelet GPIbα ectodomain shedding.
OBJECTIVE
This study was aimed to investigate the regulatory effect of protein disulfide isomerase (PDI) on platelet GPIbα ectodomain shedding.
METHODS
The washed platelets were obtained from healthy volunteers. Platelets were incubated with PDI inhibitor bacitracin before stimulation with PMA (Phorbol-12-myristate-13-acetate), dibucaine and collagen. The N-terminal domain of GPIbα in supernatant was detected by Western blot, the GPIbα expression and the intraplatelet ROS levels were measured by flow cytometry.
RESULTS
neither GC content nor GPIbα expression was changed after the washed platelets from the healthy donors were incubated only with PDI inhibitor. The washed platelets were incubated with PDI inhibitor before stimulation with different stimulin, PMA, dibucaine or collagen, and then GPIbα was cleaved and ROS levels were elevated more than that in the controls.
CONCLUSION
PDI participates in the induced GPIbα ectodomein shedding, and the effect of PDI in this process maybe depend on the change of ROS level inside platelets. These results might provide a new point of view for the platelet drug development.
Topics: Blood Platelets; Collagen; Flow Cytometry; Humans; Platelet Glycoprotein GPIb-IX Complex; Protein Disulfide-Isomerases
PubMed: 26314448
DOI: 10.7534/j.issn.1009-2137.2015.04.032 -
Annales de Dermatologie Et de... Oct 2015
Topics: Aged, 80 and over; Anesthetics, Local; Benzocaine; Conjunctivitis; Dermatitis, Allergic Contact; Dibucaine; Digital Rectal Examination; Eczema; Humans; Lubricants; Male; Ophthalmic Solutions; Patch Tests; Recurrence; Tetracaine
PubMed: 26283423
DOI: 10.1016/j.annder.2015.07.002