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Surgical Infections Oct 2014Clostridum difficile is a gram-positive, spore-forming anaerobic bacillus that has substantial associated morbidity, mortality, and associated healthcare burdens....
BACKGROUND
Clostridum difficile is a gram-positive, spore-forming anaerobic bacillus that has substantial associated morbidity, mortality, and associated healthcare burdens. Clostridium difficile spores are not destroyed by alcohol. Alcohol gel dispensers are used commonly as the hand sanitization method of choice in hospitals. It is possible that gel dispensers are fomites for C. difficile.
METHODS
Thirty alcohol-based gel dispenser handles outside of rooms of patients with active C. difficile infection were sampled. The samples were assessed for C. difficile by both culture and polymerase chain reaction (PCR). The samples were also assessed for other organisms by culture.
RESULTS
No C. difficile was cultured or detected by PCR on any of the gel dispensers. Coagulase-negative Staphyloccus spp., diptheroids, and Bacillus spp. were the organisms detected most commonly.
CONCLUSION
At our institution, C. difficile is not present on alcohol-based gel dispensers, but other potentially pathogenis are.
Topics: Academic Medical Centers; Bacteria; Clostridioides difficile; Enterocolitis, Pseudomembranous; Environmental Microbiology; Fomites; Hand Disinfection; Hand Sanitizers; Humans; Polymerase Chain Reaction
PubMed: 25126976
DOI: 10.1089/sur.2013.102 -
Journal of Infection Prevention Jan 2019The objectives of our study were to study the stethoscope cleaning practices of medical personnel and to assess the microbial load on stethoscopes and efficacy of...
BACKGROUND
The objectives of our study were to study the stethoscope cleaning practices of medical personnel and to assess the microbial load on stethoscopes and efficacy of cleaning stethoscopes with alcohol-based disinfectant.
METHODOLOGY
A questionnaire-based cross-sectional study was conducted among medical personnel at a tertiary care hospital in India to assess their knowledge and cleaning practices regarding stethoscope disinfection. Samples from the stethoscopes were collected before and after cleaning with alcohol-based disinfectant.
RESULTS
Out of 62 participants, 53.22% individuals had never cleaned their stethoscope. All the initial swab samples showed bacterial growth. There was mixed growth in the samples taken from 35 stethoscope bells and 31 diaphragms. Bacteria included coagulase negative staphylococci, bacillus species, diptheroids, and After cleaning with an alcohol-based disinfectant, there was a significant decrease in the average number of bacterial colony-forming units.
CONCLUSIONS
Regular cleaning practices should be followed to prevent growth and transmission of potentially pathogenic organisms.
PubMed: 30719088
DOI: 10.1177/1757177418802353