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Blood Purification 2021The acute kidney injury (AKI) risk score helps detect moderate and severe AKI in the next 12-24 h. However, inappropriate urine collection may impact its results.
BACKGROUND
The acute kidney injury (AKI) risk score helps detect moderate and severe AKI in the next 12-24 h. However, inappropriate urine collection may impact its results.
AIM
The aim of this study was to evaluate the stability of NephroCheck® after urine storage at different temperatures.
METHODS
The urine sample was centrifuged and split into 3 tubes. One was tested as soon as possible by the laboratory. The other 2 samples were frozen at -20 and -80°C, and the NephroCheck® test was performed 8 weeks later.
RESULTS
The mean values of the AKI risk score were 1.19 ± 0.93, 1.15 ± 1.14, and 1.20 ± 1.11 (ng/mL)2/1,000 for fresh urine, -20, and -80°C, respectively (p = 0.70). Spearman's rank correlation for -20 and -80°C versus immediate processing was strong with a rho of 0.82 and 0.98, respectively.
CONCLUSION
The AKI risk score was relatively stable. Urine could be collected without dry ice or liquid nitrogen and kept for up to 8 weeks with either -20 or -80°C freezing with stable NephroCheck® results.
Topics: Acute Kidney Injury; Biomarkers; Cold Temperature; Humans; Insulin-Like Growth Factor Binding Proteins; Tissue Inhibitor of Metalloproteinase-2; Urinalysis; Urine Specimen Collection
PubMed: 33291111
DOI: 10.1159/000511727 -
Journal of the Advanced Practitioner in... Mar 2019Ms. X is a 23-year-old female who presented to clinic with stage 2A endometrial cancer of the ovary. At her first visit, Ms. X was prescribed paclitaxel and carboplatin... (Review)
Review
Ms. X is a 23-year-old female who presented to clinic with stage 2A endometrial cancer of the ovary. At her first visit, Ms. X was prescribed paclitaxel and carboplatin on day 1 for 6 cycles. During the visit, the nurse noted Ms. X could not keep her hands out of her hair; she continually played with it. The nurse, judging by Ms. X's body language, suspected she valued her hair. Ms. X revealed she had been doing some research and wanted to use a cold cap to try and prevent alopecia during her treatment. On the first day of chemotherapy, Ms. X came to the clinic with her mother. They brought a manual cap for scalp cooling and a cooler of dry ice. Her mother was to serve as a "capper" and change the cap at 20- to 30-minute intervals during treatment to keep her scalp cool. Ms. X was made comfortable in an infusion bed, and the cap was applied 30 minutes prior to the start of therapy. Ms. X's mother changed the dry ice caps every 20 minutes throughout the infusion. Ms. X then left it on for 90 minutes following her chemotherapy. After 6 cycles of chemotherapy, Ms. X still had all her hair. She appeared to have retained 100% of her hair, although she estimates that she lost about 5% of her hair. During therapy, she followed the instructions outlined in Table 1. Ms. X reported that she rented the cap for $500 per month and paid $45 per week for the dry ice. Her mother also had to miss work to be the "capper," and this added to the out-of-pocket costs of scalp cooling.
PubMed: 31538026
DOI: No ID Found -
Methods in Molecular Biology (Clifton,... 2019The packaging and shipment of biospecimens is a multistep process for which a distinct set of regulations needs to be followed, depending on whether a biospecimen is... (Review)
Review
The packaging and shipment of biospecimens is a multistep process for which a distinct set of regulations needs to be followed, depending on whether a biospecimen is shipped domestically or internationally and whether the shipment contains hazardous materials. Shipments may be delayed if these regulations are not followed. Once learned, the process is straightforward. Major principles include double or triple packaging, adequate absorbent material, appropriate coolant, accurate labeling, and complete documentation. Training in packaging and shipping is often offered at major biomedical institutions and is a requirement to avoid shipping biohazards.
Topics: Biological Specimen Banks; Humans; Specimen Handling; Transportation
PubMed: 30539463
DOI: 10.1007/978-1-4939-8935-5_35 -
Biotechnology and Bioengineering Nov 2022As opposed to remarkable advances in the cell therapy industry, research reveal inexplicable difficulties associated with preserving and post-thawing cell death. Post... (Review)
Review
As opposed to remarkable advances in the cell therapy industry, research reveal inexplicable difficulties associated with preserving and post-thawing cell death. Post cryopreservation apoptosis is a common occurrence that has attracted the attention of scientists to use apoptosis inhibitors. Transporting cells without compromising their survival and function is crucial for any experimental cell-based therapy. Preservation of cells allows the safe transportation of cells between distances and improves quality control testing in clinical and research applications. The vitality of transported cells is used to evaluate the efficacy of transportation strategies. For many decades, the conventional global methods of cell transfer were not only expensive but also challenging and had adverse effects. The first determination of some projects is optimizing cell survival after cryopreservation. The new generation of cryopreservation science wishes to find appropriate and alternative methods for cell transportation to ship viable cells at an ambient temperature without dry ice or in media-filled flasks. The diversity of cell therapies demands new cell shipping methodologies and cryoprotectants. In this review, we tried to summarize novel improved cryopreservation methods and alternatives to cryopreservation with safe and viable cell shipping at ambient temperature, including dry preservation, hypothermic preservation, gel-based methods, encapsulation methods, fibrin microbeads, and osmolyte solution compositions.
Topics: Cell Survival; Cryopreservation; Cryoprotective Agents; Dry Ice; Fibrin; Postal Service
PubMed: 35898166
DOI: 10.1002/bit.28197 -
Microorganisms Oct 2020The microbial deterioration of cultural heritage includes physical and chemical damage as well as aesthetic alteration. With the technological advancement, a plethora of... (Review)
Review
The microbial deterioration of cultural heritage includes physical and chemical damage as well as aesthetic alteration. With the technological advancement, a plethora of techniques for removing unwanted microorganisms have opened up new opportunities for microbiologists and conservators. This article reviews the most applied, up-to-date, and sustainable techniques developed for the control of cultural heritage microbial deterioration presenting noteworthy case studies. These techniques include chemical methods, i.e., traditional biocides and nanoparticles; physical methods, such as mechanical removal, UV irradiation, gamma radiation, laser cleaning, heat shocking, microwaves, and dry ice treatment; and biological methods, such as natural molecules with biocidal activity, enzymes, and microorganisms. The application of control systems requires the comprehension of their behavior toward the unwanted microorganisms and possible interactions with the heritage materials. This overview shows also the control methods drawbacks for the purpose of creating awareness in selecting the most suitable technique or combination of techniques.
PubMed: 33036341
DOI: 10.3390/microorganisms8101542 -
Advances in Experimental Medicine and... 2018Food is a multicomponent system that mainly comprises protein, carbohydrate, fat, and water. During food processing and preservation, various physical changes (e.g.,... (Review)
Review
Food is a multicomponent system that mainly comprises protein, carbohydrate, fat, and water. During food processing and preservation, various physical changes (e.g., melting, crystallization, glass transition) occur in food products, affecting their quality. This chapter specifically examines the effect of physical changes on the quality of dry and frozen food products. Dry food products are commonly in an amorphous state. Therefore, glass transition occurs during their dehydration-rehydration processing. To control their texture and physical stability, it is important to elucidate the effects of water contents on the glass transition temperature of dry food products. Frozen foods consist of ice crystals and freeze-concentrated matrix. The formation of ice crystal and the dynamics of ice crystal evolution affect food quality. Therefore control of ice crystals is important for high-quality frozen food. Moreover, because freeze-concentrated matrix consists of solute that are plasticized by the unfrozen water and is in an amorphous state, it can undergo glass transition by freeze concentration. The physical state of freeze-concentrated matrix also strongly affects the stability of food quality during frozen storage.
Topics: Crystallization; Desiccation; Food Additives; Food Handling; Food Preservation; Food Quality; Freezing; Frozen Foods; Ice; Plasticizers; Vitrification; Water
PubMed: 30288721
DOI: 10.1007/978-981-13-1244-1_21 -
Animals : An Open Access Journal From... May 2024To address the safety problems posed by the transportation of boar semen using LN, this study was conducted on the short-term storage of frozen boar semen in dry ice...
To address the safety problems posed by the transportation of boar semen using LN, this study was conducted on the short-term storage of frozen boar semen in dry ice (-79 °C). Boar semen frozen in LN was transferred to dry ice, kept for 1 day, 3 days, 5 days, 7 days, or 8 days, and then moved back to LN. The quality of frozen semen stored in LN or dry ice was determined to evaluate the feasibility of short-distance transportation with dry ice. The results showed that 60 °C for 8 s was the best condition for thawing frozen semen stored in dry ice. No significant differences in spermatozoa motility, plasma membrane integrity, or acrosome integrity were observed in semen after short-term storage in dry ice compared to LN ( > 0.05). There were no significant changes in antioxidant properties between storage groups either ( > 0.05). In conclusion, dry ice could be used as a cold source for the short-term transportation of frozen boar semen for at least 7 days, without affecting sperm motility, morphological integrity, or antioxidant indices.
PubMed: 38791640
DOI: 10.3390/ani14101422 -
The National Medical Journal of India 2022
Topics: Humans; Dry Ice; Frostbite
PubMed: 37167517
DOI: 10.25259/NMJI_35_6_374 -
British Poultry Science Apr 20181. The objective of this study was to evaluate the effects of dry-ice decontamination on equipment and carcase surfaces in a poultry slaughterhouse and to present an...
1. The objective of this study was to evaluate the effects of dry-ice decontamination on equipment and carcase surfaces in a poultry slaughterhouse and to present an effective alternative method to the conventional decontamination processes. 2. Appreciable reductions occurred in total aerobic mesophilic bacterial counts of surface swab samples treated with dry ice (maximum difference 3.92 log cfu/100 cm). 3. After dry-ice treatment, Listeria spp. were detected on surfaces of pluckers and chiller cylinders, whereas Salmonella spp. were totally inhibited. 4. A dry-ice spraying application was more effective than a dry-ice immersing application on total aerobic mesophilic bacteria and yeast and mould counts on poultry carcases. 5. Dry-ice treatment has advantages over conventional processes. Unlike other decontamination techniques, there are no residues, so no need to wash off chemical residues from surfaces as it removes contaminants effortlessly and is environmentally friendly. 6. Dry-ice blasting of production equipment can reduce the microbial load and has potential for use in the poultry industry.
Topics: Abattoirs; Animal Husbandry; Animals; Bacteria, Aerobic; Chickens; Colony Count, Microbial; Decontamination; Dry Ice; Food Handling; Food Microbiology; Listeria; Meat; Salmonella
PubMed: 29160720
DOI: 10.1080/00071668.2017.1403565 -
In Vitro Cellular & Developmental... Dec 2017Successfully recovering stored cryopreserved cells requires attention to two major factors, i.e., storage temperature and proper handling of the frozen samples. These... (Review)
Review
Successfully recovering stored cryopreserved cells requires attention to two major factors, i.e., storage temperature and proper handling of the frozen samples. These key considerations are in turn impacted by how the inventory system is constructed, the frequency and process for retrieving specimens, as well as how the material is secured. In addition, shipping biological materials requires attention to the type of material transported, adherence to regulatory requirements, packaging materials and proper assembly, labeling, and engaging reputable carriers. This paper provides an overview discussion of the current best practices for storing, handling, and shipping of cryopreserved cells and provides references to more detailed information on specific topics.
Topics: Animals; Cells; Containment of Biohazards; Cryopreservation; Dry Ice; Humans; Nitrogen; Product Packaging; Specimen Handling; Temperature; Transportation
PubMed: 29238935
DOI: 10.1007/s11626-017-0214-6