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Lasers in Medical Science Jun 2022Evaluate the effects of antimicrobial photodynamic therapy (aPDT) and photobiomodulation (PBM) as alternatives in the treatment of mastitis in sheep. A total of 100...
Evaluate the effects of antimicrobial photodynamic therapy (aPDT) and photobiomodulation (PBM) as alternatives in the treatment of mastitis in sheep. A total of 100 sheep were evaluated, and four teats with clinical mastitis and 16 teats with subclinical mastitis were selected. Milk was collected for isolation and identification of microorganisms. They were grown on TSA, EMB, and MacConkey agar for 24 h, and the microorganisms were identified by Gram stain and biochemical tests. The ceilings were subdivided into four groups: G1, treatment with photosensitizer; G2, treatment with PBM (diode laser λ = 660 nm); G3, aPDT with methylene blue, and G4, control group. Milk samples were collected before, 24 and 48 h after treatments. Cases of subclinical mastitis presented coagulase-negative Staphylococcus and Streptococcus spp, and clinical mastitis had Escherichia coli grow from the samples. The treatments decrease the total bacterial count of negative coagulase Staphylococcus, Streptococcus spp, and Escherichia coli. Comparing the treatments, aPDT stood out, as it was able to photoinactivate all bacteria. Treatment with methylene blue photosensitizer, PBM, and aPDT induced the initial microbial reduction, but aPDT was more effective 48 h after treatment.
Topics: Animals; Anti-Bacterial Agents; Anti-Infective Agents; Coagulase; Escherichia coli; Female; Humans; Mastitis; Methylene Blue; Photochemotherapy; Photosensitizing Agents; Sheep; Streptococcus
PubMed: 35031932
DOI: 10.1007/s10103-022-03506-2 -
The Breast Journal Mar 2021A young woman presented with worsening bilateral periareolar mastitis associated with skin necrosis and delayed vesical formation after oral nipple manipulation by her...
A young woman presented with worsening bilateral periareolar mastitis associated with skin necrosis and delayed vesical formation after oral nipple manipulation by her sexual partner. After multiple failed antibiotic regimens, she was diagnosed with herpes simplex virus 1 (HSV-1) infection. This case demonstrates an uncommon etiology of breast mastitis. We highlight the importance of a timely diagnosis and early administration of antiviral therapy.
Topics: Acyclovir; Adult; Antiviral Agents; Breast Neoplasms; Female; Herpes Simplex; Herpesvirus 1, Human; Humans; Mastitis
PubMed: 33527557
DOI: 10.1111/tbj.14169 -
International Immunopharmacology Sep 2023Mastitis occurs frequently in breastfeeding women and not only affects the women's health but also hinders breastfeeding. Maslinic acid is a type of pentacyclic...
Maslinic acid alleviates LPS-induced mice mastitis by inhibiting inflammatory response, maintaining the integrity of the blood-milk barrier and regulating intestinal flora.
Mastitis occurs frequently in breastfeeding women and not only affects the women's health but also hinders breastfeeding. Maslinic acid is a type of pentacyclic triterpenoid widely found in olives that has good anti-inflammatory activity. This study aims to discuss the protective function of maslinic acid against mastitis and its underlying mechanism. For this, mice models of mastitis were established using lipopolysaccharide (LPS). The results revealed that maslinic acid reduced the pathological lesions in the mammary gland. In addition, it reduced the generation of pro-inflammatory factors and enzymes (IL-6, IL-1β, TNF-α, iNOS, and COX2) in both mice mammary tissue and mammary epithelial cells. The high-throughput 16S rDNA sequencing of intestinal flora showed that in mice with mastitis, maslinic acid treatment altered β-diversity and regulated microbial structure by increasing the abundance of probiotics such as Enterobacteriaceae and downregulating harmful bacteria such as Streptococcaceae. In addition, maslinic acid protected the blood-milk barrier by maintaining tight-junction protein expression. Furthermore, maslinic acid downregulated mammary inflammation by inhibiting the activation of NLRP3 inflammasome, AKT/NF-κB, and MAPK signaling pathways. Thus, in a mice model of LPS-induced mastitis, maslinic acid can inhibit the inflammatory response, protect the blood-milk barrier, and regulate the constitution of intestinal flora.
Topics: Humans; Female; Animals; Mice; Lipopolysaccharides; Milk; Gastrointestinal Microbiome; Mastitis; NF-kappa B; Mammary Glands, Animal
PubMed: 37406397
DOI: 10.1016/j.intimp.2023.110551 -
Life Sciences Apr 2024The World Health Organization recommends breastfeeding for 6 months, but mastitis, a common disease during lactation, presents a major obstacle to fulfilling this...
The World Health Organization recommends breastfeeding for 6 months, but mastitis, a common disease during lactation, presents a major obstacle to fulfilling this recommendation. Maternal nutrient intake during lactation has been shown to be related to mastitis. Therefore, this study aimed to explore the effect of hesperetin, a phytonutrient, on mastitis. The oral administration of hesperetin to lipopolysaccharide (LPS)-induced mastitis mice alleviated their pathological damage, reduced the secretion of pro-inflammatory cytokines, and maintained the integrity of their blood-milk barrier. Moreover, our results showed that oral administration of hesperetin regulates the composition of the intestinal flora of mice. Fecal microbial transplantation (FMT) from the mice of hesperetin group alleviated LPS-induced mastitis in recipient mice. In additional, hesperetin attenuated the inflammatory response and increased the expression of tight junction proteins (TJs) in LPS-stimulated mouse mammary epithelial cells (mMECs). Through network pharmacological analysis and further research, we demonstrated hesperetin inhibits the expression of TLR4 and the activation of NF-κB signaling. In conclusion, hesperetin protects the blood-milk barrier and improve mastitis by regulating intestinal flora and inhibiting the activation of TLR4/NF-κB signaling axis. This study provides a theoretical basis for lactating females to consume hesperetin as a supplement to prevent mastitis and maintain mammary health.
Topics: Humans; Female; Animals; Mice; NF-kappa B; Toll-Like Receptor 4; Milk; Lactation; Lipopolysaccharides; Gastrointestinal Microbiome; Mastitis; Mammary Glands, Animal; Hesperidin
PubMed: 38428570
DOI: 10.1016/j.lfs.2024.122533 -
Veterinary Journal (London, England :... Oct 2021Streptococcus uberis (S. uberis) is a mastitis pathogen with an environmental reservoir. Management factors related to housing design and bedding are associated with the...
Streptococcus uberis (S. uberis) is a mastitis pathogen with an environmental reservoir. Management factors related to housing design and bedding are associated with the risk of S. uberis mastitis. This study aimed to investigate the ability of five distinct strains of S. uberis to survive and replicate on three common bedding materials (sand, wheat straw and kiln dried pine sawdust). Sterilized bedding substrates were inoculated with S. uberis and incubated at room temperature. Bacterial recovery from these media over time indicated that S. uberis numbers increased on used bedding materials, suggesting the addition of faeces and urine promoted replication. The bacterium was recovered for at least 35 days on straw and sand bedding, but could not be recovered beyond 7 days on clean or used sawdust. This study demonstrates the importance of bedding type and management on the environmental survival of S. uberis.
Topics: Animals; Bedding and Linens; Cattle; Cattle Diseases; Female; Mastitis; Mastitis, Bovine; Streptococcus
PubMed: 34391916
DOI: 10.1016/j.tvjl.2021.105731 -
International Journal of Molecular... Apr 2019Nowadays, mastitis is one of the biggest problems in breeding dairy cattle. Treatment of this disease with conventional antibiotics is ineffective because many pathogens...
Nowadays, mastitis is one of the biggest problems in breeding dairy cattle. Treatment of this disease with conventional antibiotics is ineffective because many pathogens are resistant. Researchers have therefore been forced to look for new solutions, and metal nanoparticles (NPs) have been found to be the most appropriate agents. This study uses commercially available silver (AgNPs) and copper (CuNPs) nanoparticles and synthetized silver-copper nanoparticles (AgCuNPs) to evaluate the effect of these NPs on human and bovine mammary cells. The effect of AgNPs, CuNPs, and AgCuNPs on pathogen species commonly involved in udder inflammation (e.g., and ) was also established. The results show that commercially available NPs were of good quality and did not have a toxic effect on mammary gland tissue. AgNPs, CuNPs, and AgCuNPs also influenced or decreased the viability of pathogens. Therefore, the presented data suggest that metal NPs could be used in mastitis prevention and treatment in the future. However, the presented preliminary results require further in vivo analysis.
Topics: Animals; Bacteria; Cattle; Cell Line; Cell Membrane; Cell Survival; Copper; Epithelial Cells; Female; Fungi; Humans; Mastitis; Metal Nanoparticles; Oxidation-Reduction; Particle Size; Silver; Static Electricity
PubMed: 30987188
DOI: 10.3390/ijms20071672 -
Tropical Animal Health and Production Jul 2022This study was carried out to determine the risk factors implicated in camel subclinical mastitis as well as the physico-mineral features of camel milk, there by...
This study was carried out to determine the risk factors implicated in camel subclinical mastitis as well as the physico-mineral features of camel milk, there by examining the effect of this disease on those parameters. Milk samples were obtained from 125 lactating multiparous she-camels (aged 10-12 years) during early lactation from December 2020 to April 2021. The samples were screened for the presence of subclinical mastitis using the somatic cell count (SCC) test. In total, 73 (58.4%) samples showed the presence of subclinical mastitis, whereas the remaining 52 (41.6%) samples showed negative results for subclinical mastitis. Standard methods were used to appreciate the pH and mineral composition of the milk samples, such as Ca, Na, Cl, and K contents. In camels, unhygienic udder condition was identified as the major risk factor for the incidence of subclinical mastitis. Calf mortality and increased herd size were associated with a higher prevalence of subclinical mastitis. Tick infestations were also significantly associated with increased disease risk. Use of antisuckling devices, increase in milk yield, and weakness of body condition were identified as main risk factors and were primarily associated with the disease. However, udder lesions and deformities, milking frequency, and owner visits had no significant influence on the occurrence of subclinical mastitis. The overall mean pH value was 6.54 ± 0.01 and the mean values of Ca, Na, Cl, and K contents were 148.24 ± 2.55, 53.83 ± 1.52, 124.99 ± 1.80, and 240.22 ± 3.80 mg/100 g, respectively. When the level of milk from healthy animals and that from camels with subclinical mastitis (SCC > 2.4 × 10) were compared, it showed that Na and Cl contents were significantly increased (p < 0.05) with the increase in SCC (56.52 vs.50.07 and 128.26 vs.120.40 mg/100 g, respectively). In contrast, there were no notable changes (p > 0.05) in the Ca, K, and pH values. In conclusion, udder hygiene, calf mortality, herd size, tick infestations, use of antisuckling devices, higher milk yield, and body condition were identified as the major risk factors that predispose the camels to subclinical mastitis. Furthermore, an increase in the Na and Cl levels in camel milk was observed along with udder inflammation. Consequently, the elevated Na and Cl levels in milk can be a sign of subclinical mastitis in camels.
Topics: Animals; Camelus; Female; Lactation; Mastitis; Milk; Minerals; Risk Factors; Tick Infestations
PubMed: 35788910
DOI: 10.1007/s11250-022-03220-9 -
JAAPA : Official Journal of the... Nov 2021
Topics: Abscess; Female; Humans; Mastitis
PubMed: 34699452
DOI: 10.1097/01.JAA.0000795040.66115.51 -
Journal of Women's Health (2002) May 2023Mastitis is a common disorder among postpartum women. The discomfort and pain caused by mastitis may lead to the discontinuation of breastfeeding. Large-scale...
Mastitis is a common disorder among postpartum women. The discomfort and pain caused by mastitis may lead to the discontinuation of breastfeeding. Large-scale epidemiological studies examining mastitis are limited. Accordingly, the present study used a nationwide population-based database to collect information about all postpartum women in Taiwan to determine the incidence of and related factors for mastitis. This retrospective population-based study used the National Health Insurance Research Database to collect records of patients with mastitis during 2008-2017 and then linked the collected data to the Taiwan Birth Registry. We included women diagnosed as having lactational mastitis within 6 months of delivery. A multivariable logistic regression model was used to compare the risk of mastitis between parity in multiparous women. We identified 1,686,167 deliveries in 1,204,544 women. 19,794 women with 20,163 deliveries had a medical claim for mastitis. The incidence proportion of mastitis for 6 months postpartum was ∼1.19% and highest during the first month after delivery. Multivariable logistic regression revealed that multiparous women with a history of mastitis were likely to experience mastitis again after subsequent deliveries (adjusted odds ratio = 5.86; 95% confidence interval = 5.21-6.58). The Kaplan-Meier curve indicated that primiparous women had a higher risk of mastitis than did multiparous women (log-rank test, < 0.001). Mastitis generally occurred during the first month postpartum, and primiparous women had a higher risk of mastitis than did multiparous women. Furthermore, multiparous women with a history of mastitis had a 5.86-fold increased risk of recurrence during subsequent deliveries.
Topics: Pregnancy; Female; Humans; Incidence; Retrospective Studies; Taiwan; Postpartum Period; Mastitis
PubMed: 36897761
DOI: 10.1089/jwh.2022.0303 -
Polish Journal of Veterinary Sciences Mar 2018Staphylococci are very common human and animal pathogens. A variety of staphylococcal virulence determinates leads to vast range of infections. One of them is mastitis... (Review)
Review
Staphylococci are very common human and animal pathogens. A variety of staphylococcal virulence determinates leads to vast range of infections. One of them is mastitis which is a common disease of the mammary glands. The incidence of this disease is widespread all over the world and depends on bacterial virulence and on prevention programs. The influence of mastitis on human health is not globally evaluated, however, in veterinary fields loses in milk production caused by bovine mastitis are a constant economic problem. One of the most important parts of the mastitis control programs is accurate diagnosis of the inflammation and characterization of the etiological factors which leads to reduction of mastitis spread. Recent reports show that staphylococci are common bacterial etiological factors of mastitis, and this paper is an overview of the diagnostic typing methods used for characterization of staphylococcal isolates. A number of different techniques available to applicate is described. Phenotypic methods to identify and to differentiate isolates or discriminate virulence factors are still in use, however, some advanced genetic methods offering higher discriminatory power are reported as more accurate. In fact, nowadays the most powerful tool on that field is next generation sequencing (NGS) of the whole genome, but its high cost and requirement of special laboratory equipment makes it hard to use for routine diagnostics. That is why standard PCR techniques-based methods, and the sequencing of particular genes, are mostly used for typing bacterial isolates. Most of these techniques are characterized by a high discriminatory power, big epidemiological concordance, and repeatable results. The presented report describes the techniques used most frequent in mastitis diagnostics related to staphylococci typing and shows their advantages and disadvantages.
Topics: Animals; Bacterial Typing Techniques; Female; Mastitis; Staphylococcal Infections; Staphylococcus
PubMed: 29624017
DOI: 10.24425/119041