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Automated Detection and Diameter Estimation for Mouse Mesenteric Artery Using Semantic Segmentation.Journal of Vascular Research 2021Pressurized myography is useful for the assessment of small artery structures and function. However, this procedure requires technical expertise for sample preparation...
BACKGROUND
Pressurized myography is useful for the assessment of small artery structures and function. However, this procedure requires technical expertise for sample preparation and effort to choose an appropriate sized artery. In this study, we developed an automatic artery/vein differentiation and a size measurement system utilizing machine learning algorithms.
METHODS AND RESULTS
We used 654 independent mouse mesenteric artery images for model training. The model yielded an Intersection-over-Union of 0.744 ± 0.031 and a Dice coefficient of 0.881 ± 0.016. The vessel size and lumen size calculated from the predicted vessel contours demonstrated a strong linear correlation with manually determined vessel sizes (R = 0.722 ± 0.048, p < 0.001 for vessel size and R = 0.908 ± 0.027, p < 0.001 for lumen size). Last, we assessed the relation between the vessel size before and after dissection using a pressurized myography system. We observed a strong positive correlation between the wall/lumen ratio before dissection and the lumen expansion ratio (R = 0.832, p < 0.01). Using multivariate binary logistic regression, 2 models estimating whether the vessel met the size criteria (lumen size of 160-240 μm) were generated with an area under the receiver operating characteristic curve of 0.761 for the upper limit and 0.747 for the lower limit.
CONCLUSION
The U-Net-based image analysis method could streamline the experimental approach.
Topics: Animals; Arterial Pressure; Automation; Female; Genotype; Image Interpretation, Computer-Assisted; Machine Learning; Male; Mesenteric Arteries; Mesenteric Veins; Mice, Inbred C57BL; Mice, Transgenic; Microscopy; Myography; Neural Networks, Computer; Phenotype; Predictive Value of Tests; Mice
PubMed: 34182554
DOI: 10.1159/000516842 -
Ugeskrift For Laeger Apr 2016
Topics: Computed Tomography Angiography; Esophageal and Gastric Varices; Gastrointestinal Hemorrhage; Humans; Male; Mesenteric Veins; Middle Aged; Radionuclide Imaging; Varicose Veins
PubMed: 27136951
DOI: No ID Found -
Vascular Pharmacology Apr 2021Chronic venous insufficiency (CVI) is a common disorder associated with a variety of symptoms in later disease stages; despite the high prevalence of this pathology,...
Chronic venous insufficiency (CVI) is a common disorder associated with a variety of symptoms in later disease stages; despite the high prevalence of this pathology, suitable pharmaceutical therapies have not been explored to date. In this context, it was recently reported that a chronic increase in venous wall stress or biomechanical stretch is sufficient to cause development of varicose veins. Recent evidence demonstrate that flavonoids are natural substances that convey the circulatory system functionality, playing a key role in blood flow. Particularly, troxerutin, diosmin and horse chestnut extract, appear protective for the management of vascular diseases. The aim of the present study was to evaluate the effect of a flavonoid compound, containing troxerutin, diosmin and horse chestnut extract on in vitro model on HUVECs cells, due to its production of vasculoregulatory and vasculotropic molecules, on an ex-vivo model on mesenteric vessel contraction, to regularize mesenteric microcirculation and on in vivo model of CVI-induced by saphene vein ligation. Furthermore, the flavonoid compound capacity of extensibility and compatibility with peripheral veins was investigated through a tissue block culture study. The degree of absorption, the contractile venous activity, the histological analysis, the immunoistochemical and immunofluorescence evaluation for VEGF and CD34 were performed, together with inflammatory mediators dosage. For the first time, this research revealed the therapeutic potential of a compound, enriched with flavonoids, to be a supportive treatment, suitable to reduce varicose vein pathophysiology and to regularize venous tone.
Topics: Animals; Cardiovascular Agents; Cells, Cultured; Chronic Disease; Cytokines; Disease Models, Animal; Flavonoids; Human Umbilical Vein Endothelial Cells; Humans; Male; Mesenteric Veins; Mice; Nitric Oxide Synthase Type III; Saphenous Vein; Vascular Endothelial Growth Factor A; Vasoconstriction; Venous Insufficiency
PubMed: 33278582
DOI: 10.1016/j.vph.2020.106825 -
Annals of Surgical Oncology Jan 2018Pancreatic cancer continues to carry a dismal prognosis with the majority of patients presenting at advanced stages of disease. Complete surgical resection remains... (Review)
Review
Pancreatic cancer continues to carry a dismal prognosis with the majority of patients presenting at advanced stages of disease. Complete surgical resection remains essential for prolonging survival and increasing the possibility of cure. However, few patients will be resectable at diagnosis, with a significant portion presenting with borderline or locally advanced disease. The addition of vascular resection and reconstruction at the time of pancreatectomy enables expansion of the patient population able to undergo resection with curative intent and achieve tumor-free margins. This review provides an overview of the literature regarding the role of venous and arterial resection in the treatment of pancreatic cancer, with a focus on outcomes including survival, morbidity, and mortality.
Topics: Arteries; Chemotherapy, Adjuvant; Humans; Mesenteric Veins; Neoadjuvant Therapy; Pancreatectomy; Pancreatic Neoplasms; Pancreaticoduodenectomy; Portal Vein; Plastic Surgery Procedures; Vascular Surgical Procedures
PubMed: 27882472
DOI: 10.1245/s10434-016-5676-3 -
Abdominal Imaging Jun 2015
Review
Topics: Contrast Media; Crohn Disease; Diagnosis, Differential; Humans; Mesenteric Arteries; Mesenteric Veins; Radiographic Image Enhancement; Tomography, X-Ray Computed
PubMed: 25367811
DOI: 10.1007/s00261-014-0285-8 -
European Journal of Vascular and... Mar 2021
Topics: Arteriovenous Fistula; Computed Tomography Angiography; Female; Humans; Mesenteric Artery, Superior; Mesenteric Veins; Middle Aged; Phlebography
PubMed: 33262092
DOI: 10.1016/j.ejvs.2020.10.034 -
Arquivos Brasileiros de Cirurgia... 2014Due to their complexity and risks, mesenteric-portal axis resection and reconstruction during the pancreatectomy procedure were not recommended back in the early...
BACKGROUND
Due to their complexity and risks, mesenteric-portal axis resection and reconstruction during the pancreatectomy procedure were not recommended back in the early nineties. However, as per technical improvements and the reduction in morbidity and mortality rates, they have been routinely indicated in large medical centers.
AIM
To show results from cases of patients subjected to mesenteric-portal axis resection during pancreatectomy.
METHOD
Patients subjected to mesenteric-portal axis resection during pancreatectomy were prospectively and consecutively assessed. The procedure was indicated according to anatomical criteria defined by imaging exams or intraoperative assessment.
RESULTS
Ten patients, half of them were male, with mean age of 55.7 years (40-76) were included. The most frequent underlying diseases were pancreatic adenocarcinoma and Frantz tumor. The circumferential resection of the portal vein associated with the superior mesenteric vein with splenic vein ligature (4 cases=40%) and the primary anastomosis of the vascular stumps (5 cases=50%) were, respectively, the most performed types of vascular resection and reconstruction. Surgery time ranged from 480 to 600 minutes (average=556 minutes) and postoperative hospitalization time ranged from 9 to 114 days (average=34.8 days). Morbidity rate was 60%, and clinical pancreatic fistula (grade B and C) was the most common complication (3 cases=30%). Mortality rate was 10% (1 case).
CONCLUSION
Mesenteric-portal axis resection is a valid technical procedure. It should be taken into account after a clinical assessment that included not only the patients' clinical condition but also the technical and anatomical conditions of the mesenteric-portal axis tumor infiltration as well as life expectancy based on the patient's cancer prognosis.
Topics: Adult; Aged; Female; Humans; Male; Mesenteric Veins; Middle Aged; Pancreatectomy; Portal Vein; Prospective Studies; Treatment Outcome
PubMed: 25626936
DOI: 10.1590/S0102-67202014000400009 -
Updates in Surgery Sep 2016Nowadays, pancreaticoduodenectomies (PD) with an "en-bloc" resection of the spleno-mesenterico-portal (SMP) venous axis are safely performed at tertiary centers for... (Review)
Review
Nowadays, pancreaticoduodenectomies (PD) with an "en-bloc" resection of the spleno-mesenterico-portal (SMP) venous axis are safely performed at tertiary centers for patients presenting venous invasion. However, for tumors infiltrating the SMP confluence optimal management of the splenic vein (SV) remains a matter of debate. Simple SV ligation has been associated with the development of sinistral portal hypertension, gastrointestinal bleeding and hypersplenism over the long term. To avoid these complications, reconstructive methods such as the direct implantation of the SV into a SMP "neoconfluence", the inferior mesenteric vein-SV anastomosis and the distal spleno-renal shunt have been reported. This article summarizes the different technical solutions available and the current evidence supporting the optimal management of the SV stump during a "safe" radical PD for pancreatic cancer. Technical issues, advantages as well as drawbacks of the different techniques, are discussed.
Topics: Anastomosis, Surgical; Humans; Ligation; Mesenteric Veins; Pancreatic Neoplasms; Pancreaticoduodenectomy; Portal Vein; Spleen; Splenic Vein
PubMed: 27651335
DOI: 10.1007/s13304-016-0396-6 -
Radiographics : a Review Publication of... 2020Pancreatic surgery with en bloc venous resection and reconstruction is becoming increasingly common in the current era of expanding neoadjuvant oncologic therapies and... (Review)
Review
Pancreatic surgery with en bloc venous resection and reconstruction is becoming increasingly common in the current era of expanding neoadjuvant oncologic therapies and advanced surgical techniques for patients with more anatomically complex tumors. However, patients who have alterations in their venous outflow are at increased risk for postoperative portomesenteric venous stenosis and/or thrombosis. Cross-sectional imaging for postoperative surveillance, including multiphase CT or MRI, is critical for recognizing portomesenteric venous complications and thus implementing early intervention and preventing complications related to portomesenteric venous hypertension. Hypertension-related complications include ascites, variceal or gastrointestinal bleeding, postprandial abdominal pain, intestinal edema, protein-losing enteropathy, malabsorptive diarrhea, and splenomegaly. Percutaneous transhepatic, transsplenic, and transjugular portomesenteric interventions, including venoplasty, stent placement, and thrombectomy or thrombolysis, are safe and effective options for restoring patency to the portomesenteric venous system. Preintervention CT or MRI and diagnostic catheter venography are important for procedural planning, while postintervention CT or MRI surveillance is critical for detecting recurrent stenosis or thrombosis, or de novo portomesenteric venous disease. RSNA, 2020.
Topics: Humans; Mesenteric Veins; Pancreatic Neoplasms; Portal System; Postoperative Complications; Vascular Surgical Procedures
PubMed: 31977263
DOI: 10.1148/rg.2020190100 -
Diagnostic and Interventional Imaging Nov 2022
Topics: Humans; Mesenteric Veins; Diverticulitis; Colon, Sigmoid; Sigmoid Diseases; Portal Vein
PubMed: 36266193
DOI: 10.1016/j.diii.2022.09.008