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Korean Journal of Critical Care Medicine Nov 2017Although methidathion is an organophosphate insecticide, it is different from the other organophosphates in terms of toxicity. Because of its relatively high fat...
Although methidathion is an organophosphate insecticide, it is different from the other organophosphates in terms of toxicity. Because of its relatively high fat solubility, the apparent volume of methidathion distribution throughout the body is very high, indicating that hemoperfusion is not effective in removing this organophosphate from the body. Redistribution of methidathion from fat to blood can also occur when plasma levels diminish. Additionally, acetylcholinesterase aging, which is the loss of an alkyl side chain that prevents reactivation by oximes, is very rapid so that the effective reactivation by oximes is thwarted. Thus, methidathion's effect on acetylcholinesterase inhibition is long lasting, particularly with a high dose. In addition to its parasympatholytic effect and ability to induce muscle paralysis, methidathion poisoning is associated with a profound and long-lasting circulatory collapse due to sympathetic ganglion blockade. This report presents the case of a 55-year-old man who accidentally ingested a high dose of methidathion. He later developed enteroinvasive aspergillosis infection-induced multiple bowel perforations on two separate occasions while on mechanical ventilator support, resulting in a fatal outcome. The renin-angiotensin axis activated by sympathetic ganglion blockade may have reduced the patient's splanchnic blood flow, contributing to translocation of endotoxin. Also, the effect of excessive acetylcholine on non-neuronal acetylcholine receptors may have contributed to the development of fatal enteroinvasive aspergillosis in this patient.
PubMed: 31723659
DOI: 10.4266/kjccm.2016.00073 -
Pesticide Biochemistry and Physiology Aug 2023Methidathion is a highly effective organophosphorus pesticide and is extensively utilized for the control of insects in agricultural production. However, there is little...
Methidathion is a highly effective organophosphorus pesticide and is extensively utilized for the control of insects in agricultural production. However, there is little information on the adverse effects and underlying mechanisms of methidathion on aquatic organisms. In this work, embryonic zebrafish were exposed to methidathion at concentrations of 4, 10, and 25 mg/L for 96 h, and morphological changes and activities of antioxidant indicators alterations were detected. In addition, the locomotor behavioral abilities of zebrafish exposed to methidathion were also measured. To further explore the mechanism of the toxic effects of methidathion, gene expression levels associated with cardiac development, cell apoptosis, and the immune system were tested through qPCR assays. The findings revealed that methidathion exposure could induce a decrease in survival rate, hatchability, length of body, and increase in abnormality of zebrafish, as well as cardiac developmental toxicity. The LC value of methidathion in zebrafish embryos was determined to be about 30.72 mg/L at 96 hpf. Additionally, methidathion exposure triggered oxidative stress in zebrafish by increasing SOD activity, ROS, and MDA content. Acridine orange (AO) staining indicated that methidathion exposure led to apoptosis, which was mainly distributed in the pericardial region. Furthermore, significant impairments of locomotor activity in zebrafish larvae were induced by methidathion exposure. Lastly, the expression of pro-inflammatory factors including IFN-γ, IL-6, IL-8, CXCL-clc, TLR4, and MYD88 significantly up-regulated in exposed zebrafish. Taken together, the results in this work illustrated that methidathion caused developmental toxicity, cardiotoxicity, and immunotoxicity in embryogenetic zebrafish.
Topics: Animals; Zebrafish; Cardiotoxicity; Organophosphorus Compounds; Pesticides; Oxidative Stress; Embryo, Nonmammalian; Water Pollutants, Chemical
PubMed: 37532338
DOI: 10.1016/j.pestbp.2023.105526 -
The Journal of Toxicological Sciences 2022Methidathion [3-(dimethoxyphosphinothioylsulfanylmethyl)-5-methoxy-1,3,4-thiadiazol-2-one; hereinafter DMTP], one of the most widely used organophosphorus pesticides,...
Methidathion [3-(dimethoxyphosphinothioylsulfanylmethyl)-5-methoxy-1,3,4-thiadiazol-2-one; hereinafter DMTP], one of the most widely used organophosphorus pesticides, has been detected in some clinical cases of accidental exposure and suicide in Japan. It has been reported that DMTP concentration is decreased in blood. In this study, it is difficult to recover DMTP in the free form because DMTP is bound to human serum albumin (HSA). We detected DMTP adducts in HSA by liquid chromatography quadrupole time-of-flight mass spectrometry (LC-Q/TOF-MS). The mass spectra showed that DMTP was preferably bound to the lysine (K), tyrosine (Y), and cysteinylproline (CP) residues of HSA. The concentrations of K-adduct, DMTP-Y-adduct and DMTP-CP-adduct were increased in vitro in a dose-dependent fashion when DMTP concentration was lower than the lethal dose. Furthermore, the DMTP-Y-adduct and DMTP-CP-adduct were also detected in post-mortem blood of an autopsied subject who died by intentional DMTP ingestion. The results suggested that the DMTP-Y-adduct and DMTP-CP-adduct could be used as a biomarker of DMTP poisoning, and the decrease concentration of DMTP in blood after death could be determined on the basis of the concentration of the DMTP-CP-adduct in blood.
Topics: Humans; Organophosphorus Compounds; Organothiophosphorus Compounds; Pesticides; Serum Albumin, Human
PubMed: 35370241
DOI: 10.2131/jts.47.139 -
RSC Advances Jun 2023Water contamination by pesticides is a critical environmental issue, necessitating the development of sustainable and efficient degradation methods. This study focuses...
Water contamination by pesticides is a critical environmental issue, necessitating the development of sustainable and efficient degradation methods. This study focuses on synthesizing and evaluating a novel heterogeneous sonocatalyst for degrading pesticide methidathion. The catalyst consists of graphene oxide (GO) decorated CuFeO@SiO nanocomposites. Comprehensive characterization using various techniques confirmed the superior sonocatalytic activity of the CuFeO@SiO-GOCOOH nanocomposite compared to CuFeO@SiO alone. The enhanced performance is attributed to the combined effects of GO and CuFeO@SiO, including increased surface area, enhanced adsorption capabilities, and efficient electron transfer pathways. Reaction parameters such as time, temperature, concentration, and pH significantly influenced the degradation efficiency of methidathion. Longer reaction times, higher temperatures, and lower initial pesticide concentrations favored faster degradation and higher efficiency. Optimal pH conditions were identified to ensure effective degradation. Remarkably, the catalyst demonstrated excellent recyclability, indicating its potential for practical implementation in pesticide-contaminated wastewater treatment. This research contributes to the development of sustainable methods for environmental remediation, highlighting the promising potential of the graphene oxide decorated CuFeO@SiO nanocomposite as an effective heterogeneous sonocatalyst for pesticide degradation.
PubMed: 37388148
DOI: 10.1039/d3ra02773b -
Foods (Basel, Switzerland) Sep 2023Pesticide residues in kumquat fruits from China, and the quality and chronic/acute intake risks in Chinese consumers, were assessed using the QuEChERS procedure and...
Pesticide residues in kumquat fruits from China, and the quality and chronic/acute intake risks in Chinese consumers, were assessed using the QuEChERS procedure and UHPLC-MS/MS and GC-MS/MS methods. Our 5-year monitoring and survey showed 90% of the 573 samples of kumquat fruits collected from two main production areas contained one or multiple residual pesticides. Overall, 30 pesticides were detected, including 16 insecticides, 7 fungicides, 5 acaricides, and 2 plant growth modulators, of which 2 pesticides were already banned. Two or more residual pesticides were discovered in 81% of the samples, and pesticide residues in 9.4% of the samples surpassed the , such as profenofos, bifenthrin, triazophos, avermectin, spirodiclofen, difenoconazole, and methidathion. The major risk factors on the safety of kumquat fruits before 2019 were profenofos, bifenthrin, and triazophos, but their over-standard frequencies significantly declined after 2019, which was credited to the stricter supervision and management policies by local governments. Despite the high detection rates and multi-residue occurrence of pesticides in kumquat fruits, about 81% of the samples were assessed as qualified. Moreover, the accumulative chronic diet risk determined from is very low. To better protect the health of customers, we shall formulate stricter organic phosphorus pesticide control measures and stricter use guidelines, especially for methidathion, triazophos, chlorpyrifos, and profenofos. This study provides potential data for the design of kumquat fruit quality and safety control guidelines and for the reduction in health risks to humans.
PubMed: 37761133
DOI: 10.3390/foods12183423 -
Journal of Environmental Science and... Dec 2016A H-nuclear magnetic resonance (NMR) spectroscopy with multivariate analysis was applied to detect the toxicity of antiacetylcholinesterase insecticides, methomyl...
A H-nuclear magnetic resonance (NMR) spectroscopy with multivariate analysis was applied to detect the toxicity of antiacetylcholinesterase insecticides, methomyl (methyl (1E)-N-(methylcarbamoyloxy)ethanimidothioate) and methidathion (3-(dimethoxyphosphinothioyl sulfanylmethyl)-5-methoxy-1,3,4-thiadiazol-2-one), using zebrafish (Danio rerio) and Chinese bleak (Aphyocypris chinensis). Generally, methomyl and methidathion have been believed not to highly accumulate in fish tissues. However, these pesticides showed their toxicity by altering patterns of whole-body metabolites in neurotransmitter balance, energy metabolism, oxidative stress, and muscle maintenance in low concentrations. We used Pearson correlation analysis to contextualize the metabolic markers in pesticide treated groups. We observed that the positive correlations of choline with acetate and betaine in untreated control were shifted to null correlations showing acetylcholinesterase specific toxicity. This research demonstrated the applicability and potential of NMR metabolomics in detecting toxic effects of insecticide with a modicum of concentrations in aquatic environment.
Topics: Animals; Behavior, Animal; Cyprinidae; Female; Insecticides; Male; Metabolomics; Methomyl; Multivariate Analysis; Organothiophosphorus Compounds; Oxidative Stress; Pesticides; Proton Magnetic Resonance Spectroscopy; Toxicity Tests; Water Pollutants, Chemical; Zebrafish
PubMed: 27715651
DOI: 10.1080/03601234.2016.1208460 -
Toxicology Jan 2022Short-term biomarkers of toxicity have an increasingly important role in the screening and prioritization of new chemicals. In this study, we examined early indicators... (Comparative Study)
Comparative Study
Short-term biomarkers of toxicity have an increasingly important role in the screening and prioritization of new chemicals. In this study, we examined early indicators of liver toxicity for three reference organophosphate (OP) chemicals, which are among the most widely used insecticides in the world. The OP methidathion was previously shown to increase the incidence of liver toxicity, including hepatocellular tumors, in male mice. To provide insights into the adverse outcome pathway (AOP) that underlies these tumors, effects of methidathion in the male mouse liver were examined after 7 and 28 day exposures and compared to those of two other OPs that either do not increase (fenthion) or possibly suppress liver cancer (parathion) in mice. None of the chemicals caused increases in liver weight/body weight or histopathological changes in the liver. Parathion decreased liver cell proliferation after 7 and 28 days while the other chemicals had no effects. There was no evidence for hepatotoxicity in any of the treatment groups. Full-genome microarray analysis of the livers from the 7 and 28 day treatments demonstrated that methidathion and fenthion regulated a large number of overlapping genes, while parathion regulated a unique set of genes. Examination of cytochrome P450 enzyme activities and use of predictive gene expression biomarkers found no consistent evidence for activation of AhR, CAR, PXR, or PPARα. Parathion suppressed the male-specific gene expression pattern through STAT5b, similar to genetic and dietary conditions that decrease liver tumor incidence in mice. Overall, these findings indicate that methidathion causes liver cancer by a mechanism that does not involve common mechanisms of liver cancer induction.
Topics: Animals; Basic Helix-Loop-Helix Transcription Factors; Cell Transformation, Neoplastic; Chemical and Drug Induced Liver Injury; Constitutive Androstane Receptor; Cytochrome P-450 Enzyme System; Fenthion; Gene Expression Profiling; Genomics; Insecticides; Liver; Liver Neoplasms; Male; Mice; Organophosphorus Compounds; Organothiophosphorus Compounds; PPAR alpha; Parathion; Receptors, Aryl Hydrocarbon; STAT5 Transcription Factor; Transcriptome
PubMed: 34813904
DOI: 10.1016/j.tox.2021.153046 -
International Journal of Environmental... May 2020Organochlorine (OCPs) and organophosphorus pesticides (OPPs) have been intensively applied in rice paddy field farming to control pest infestation and increase the...
Organochlorine (OCPs) and organophosphorus pesticides (OPPs) have been intensively applied in rice paddy field farming to control pest infestation and increase the yield. In this study, we investigated the presence of organochlorine and organophosphorus pesticides in paddy rice and soil from rice plantations in Thailand and China. According to concentration and distribution of OCPs, the most abundant OCPs residues in rice and soil from Thailand and China were dichlorodiphenyltrichloroethane and hexachlorocyclohexanes. The OPPs of methidathion, carbophenothion, chlorpyrifos, and diazinon were common to Thailand and China in both types of samples. The detection frequency of multiple types of these pesticides was greater than 50% of total samples. The relative concentration of some OPPs residues in rice and soil from Thailand and China were significantly different from each other (p < 0.0083), whereas, no significant difference was observed for the relative concentration of OCPs residues in rice and soil from both countries, except for HCHs (p < 0.05). Bioaccumulation factors of OCPs between rice and soil samples indicated that OCPs and OPPs in soil could accumulate in rice. The carcinogenic and non- carcinogenic risks of OCPs and OPPs seem to be in the safe range as recommended by the European Union.
Topics: China; Environmental Monitoring; Humans; Hydrocarbons, Chlorinated; Oryza; Pesticides; Risk Assessment; Soil; Soil Pollutants; Thailand
PubMed: 32471043
DOI: 10.3390/ijerph17113786 -
Environmental Analysis, Health and... Dec 2022The aim of the study was to assess the occurrence and distribution of organophosphate compounds residue in soil, surface water, sediment, and banana crops in Araromi...
The aim of the study was to assess the occurrence and distribution of organophosphate compounds residue in soil, surface water, sediment, and banana crops in Araromi farm settlement, Osun State, Nigeria. Organophosphate pesticide residues were determined using a gas chromatography equipped with Flame-Ionization Detection (GC-FID) in 16 soil samples from cocoa and banana farms, 6 water and sediment samples each, and 8 banana samples from 4 farms in the study site. Fourteen organophosphate compounds were detected (acephate, omethoate, dementon-s-methyl, dimethoate, tolcofos-methyl, pirimiphos-methyl, malathion, chlorpyrifos, methidathion, prothiofos, profenofos, ethion, azinphos-methyl and pyrazophos). Tolclofos-methyl, pirimiphos-methyl and prothiofos were detected in all the soil and sediment samples with concentration ranges of 1.9-12.9, 2.25-6.98 and 3.38-9.89 mg/kg respectively in soil and 8.13-9.83, 2.82-25.1 and 3.70-19.5 mg/kg respectively in sediment. Dimethoate, pirimiphos-methyl and prothiofos with concentration ranges, 0.06-0.28, 0.09-0.18 and 0.16-6.11 mg/L respectively were mostly detected in water samples while dimethoate, tolcofos-methyl, malathion, methidathion, prothiofos, ethion and azinphos-methyl compounds were detected in all the banana samples with concentration ranges, 3.40-12.0, 1.82-6.26, 5.73-9.48, 29.7-145, 8.24-20.1, 3.87-9.35 and 3.66-12.2 mg/kg respectively. The organophosphate mean residue concentrations were mostly significantly higher than the Maximum Residue Limits (MRL) at p<0.05. Across the three samples, only pirimiphos-methyl was significantly higher in water samples, omethoate in sediment; acephate, dementon-s-methyl and chlorpyrifos in banana were also not significantly higher at p<0.05. A strong positive significant correlation was observed between the organophosphate compounds in the banana and water samples (R=0.77, p=0.002) at p<0.05. The occurrence of organophosphate compounds in concentrations above MRLs may pose serious environmental and health risks.
PubMed: 36916048
DOI: 10.5620/eaht.2022035 -
International Journal of Environmental... Jan 2022This mixed methods study used laboratory measurements of pesticide residues in produce, semi-structured questionnaires, and in-depth interview data to describe trends in...
This mixed methods study used laboratory measurements of pesticide residues in produce, semi-structured questionnaires, and in-depth interview data to describe trends in pesticide residue in produce and handling and processing practices for fruits (watermelon and passion fruit) and vegetables (tomato, cabbage, and eggplant) along the farm to fork chain. Of the 50 farmers visited, 34 (68.0%) sold their fruits and vegetables to transporters, 11 (22.0%) to market vendors, and 4 (8.0%) directly to homes and restaurants. The majority 42 (93.3%) of the consumers (home/restaurant) purchased their fruits and vegetables from market vendors and transporters. Washing with water or vinegar, wiping with a cloth, peeling the outer layer, and blending and cooking were the most common post-harvesting processing methods used by stakeholders along the supply chain. Some farmers and market vendors reported spraying fruits and vegetables with pesticides either prior- or post-harvest to increase shelf life. Statistically significant decreasing pesticide residue trends along the farm to fork chain were observed for dioxacarb, likely due to degradation or washing, peeling, cooking, blending, or wiping by consumers. Increasing trends were observed for methidathion and quinalphos possibly due to pesticide applications. There is a need in Uganda to promote practices that minimize pesticide use and exposure through diet, while maintaining food integrity.
Topics: Farms; Food Contamination; Fruit; Pesticide Residues; Uganda; Vegetables
PubMed: 35162373
DOI: 10.3390/ijerph19031350