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Clinics in Laboratory Medicine Dec 2022
Topics: Humans; Precision Medicine; Pathology, Molecular; Neoplasms
PubMed: 36368791
DOI: 10.1016/j.cll.2022.09.019 -
Trends in Biotechnology Mar 2015Duplex-specific nucleases (DSNs) are promising tools for bioanalysis because of their unique ability to cleave DNA within duplexes while keeping a single strand intact.... (Review)
Review
Duplex-specific nucleases (DSNs) are promising tools for bioanalysis because of their unique ability to cleave DNA within duplexes while keeping a single strand intact. There is prevalent use of DSNs in both biomedical and biological science applications, such as cDNA library construction, circulating miRNA detection, telomeric overhang detection, and SNP recognition. We present an overview of the current knowledge of DSNs, with special emphasis on DSN-mediated isothermal signal amplification strategies for trace miRNA detection. Continued innovation to address key challenges, such as amplification-free approaches, will open up new avenues in the field of miRNA profiling, offering opportunities for improved personalized medicine, preventive medicine, and translational medicine.
Topics: DNA; Deoxyribonucleases; Molecular Biology; Molecular Diagnostic Techniques; Molecular Medicine; Precision Medicine; RNA, Double-Stranded; Substrate Specificity
PubMed: 25640199
DOI: 10.1016/j.tibtech.2014.12.008 -
Journal of Cellular and Molecular... Feb 2017
Topics: Biomedical Research; Cell Biology; Editorial Policies; Forecasting; Humans; Journal Impact Factor; Molecular Biology; Molecular Medicine; Periodicals as Topic
PubMed: 28121074
DOI: 10.1111/jcmm.13075 -
Nature Reviews. Nephrology Dec 2023Primary aldosteronism is the most common single cause of hypertension and is potentially curable when only one adrenal gland is the culprit. The importance of primary... (Review)
Review
Primary aldosteronism is the most common single cause of hypertension and is potentially curable when only one adrenal gland is the culprit. The importance of primary aldosteronism to public health derives from its high prevalence but huge under-diagnosis (estimated to be <1% of all affected individuals), despite the consequences of poor blood pressure control by conventional therapy and enhanced cardiovascular risk. This state of affairs is attributable to the fact that the tools used for diagnosis or treatment are still those that originated in the 1970-1990s. Conversely, molecular discoveries have transformed our understanding of adrenal physiology and pathology. Many molecules and processes associated with constant adrenocortical renewal and interzonal metamorphosis also feature in aldosterone-producing adenomas and aldosterone-producing micronodules. The adrenal gland has one of the most significant rates of non-silent somatic mutations, with frequent selection of those driving autonomous aldosterone production, and distinct clinical presentations and outcomes for most genotypes. The disappearance of aldosterone synthesis and cells from most of the adult human zona glomerulosa is the likely driver of the mutational success that causes aldosterone-producing adenomas, but insights into the pathways that lead to constitutive aldosterone production and cell survival may open up opportunities for novel therapies.
Topics: Adult; Humans; Aldosterone; Hyperaldosteronism; Public Health; Molecular Medicine; Adenoma
PubMed: 37612380
DOI: 10.1038/s41581-023-00753-6 -
Seminars in Diagnostic Pathology Sep 2019Molecular diagnostic techniques are part of the ancillary arsenal of anatomic pathologists. Advances in technology and knowledge regarding disease pathogenesis,... (Review)
Review
Molecular diagnostic techniques are part of the ancillary arsenal of anatomic pathologists. Advances in technology and knowledge regarding disease pathogenesis, tumorigenesis, and immune function contribute to the development of these assays. However, each technique, if applied incorrectly or in ignorance, can lead to difficulties in execution or errors in interpretation. In this review of commonly used molecular diagnostic tests, including immunohistochemistry, microsatellite instability testing, chromosomal microarray testing, and conventional and next-generation sequencing, the emphasis will be on potential pitfalls and considerations for each platform. Emerging technologies that may be used in clinical applications in the near future will also be discussed. An understanding of the methodologies, advantages, and drawbacks of molecular assays will help pathologists aid in diagnostic and therapeutic decisions.
Topics: Humans; Molecular Diagnostic Techniques; Pathology, Molecular
PubMed: 31182318
DOI: 10.1053/j.semdp.2019.06.002 -
Clinical and Translational Medicine Jan 2021
Topics: Humans; Molecular Medicine; Precision Medicine; Translational Research, Biomedical
PubMed: 33463066
DOI: 10.1002/ctm2.294 -
Chemical Society Reviews Oct 2017
Topics: Drug Delivery Systems; Macromolecular Substances; Molecular Medicine; Thermodynamics
PubMed: 29034939
DOI: 10.1039/c7cs90102j -
Frontiers in Public Health 2023
Topics: Humans; Adolescent; Molecular Epidemiology; Neoplasms
PubMed: 37026123
DOI: 10.3389/fpubh.2023.1177707 -
International Journal of Molecular... Jul 2023After Rudolf Virchow's pioneering works, technological advances boosted the scientific interest in this research field, which nowadays is still far from extinguished...
After Rudolf Virchow's pioneering works, technological advances boosted the scientific interest in this research field, which nowadays is still far from extinguished [...].
Topics: History, 19th Century; Molecular Medicine
PubMed: 37511502
DOI: 10.3390/ijms241411743 -
Biophysical Chemistry 2016Molecular medicine is founded on the synergy between Chemistry, Physics, Biology and Medicine, with the ambitious goal of tackling diseases from a molecular perspective.... (Review)
Review
Molecular medicine is founded on the synergy between Chemistry, Physics, Biology and Medicine, with the ambitious goal of tackling diseases from a molecular perspective. This Review aims at retracing a personal outlook of the birth and development of molecular medicine, as well as at highlighting some of the most urgent challenges linked to aging and represented by incurable neurodegenerative diseases caused by protein misfolding. Furthermore, we emphasize the emerging role of the retromer dysfunctions and improper protein sorting in Alzheimer's disease and other important neurological disordered.
Topics: Aging; Humans; Molecular Medicine; Neurodegenerative Diseases; Protein Folding; Protein Transport; Vesicular Transport Proteins
PubMed: 27214761
DOI: 10.1016/j.bpc.2016.05.004