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Frontiers in Immunology 2024Chronic low-grade inflammation is an important aspect of morbidity and mortality in older adults. The level of circulating pro-inflammatory cytokines (interleukin... (Meta-Analysis)
Meta-Analysis
INTRODUCTION
Chronic low-grade inflammation is an important aspect of morbidity and mortality in older adults. The level of circulating pro-inflammatory cytokines (interleukin (IL)-6, tumor necrosis factor (TNF) or IL-1β) is a risk factor in cardiovascular and neurodegenerative diseases and is also associated with sarcopenia and frailties. The objective of this study was to assess each cytokine: IL-6, TNF, and IL-1β separately in the elderly with comorbidities against controls without diseases according to the data published in the available literature.
METHODS
The electronic bibliographic PubMed database was systematically searched to select all the relevant studies published up to July 2023. The total number of the subjects involved in the meta-analysis included patients with diseases (=8154) and controls (=33967).
RESULTS
The overall concentration of IL-6 was found to be higher in patients with diseases compared to controls and the difference was statistically significant, with a -value of <0.001 (SMD, 0.16; 95% CI, 0.12-0.19). The heterogeneity was considerable with Q = 109.97 (P <0.0001) and I = 79.2%. The potential diagnostic usefulness of IL-6 was confirmed by odds ratio (OR) analysis (OR: 1.03, 95% CI (1.01; 1.05), =0.0029). The concentration of both TNF and IL-1β was elevated in the control group compared to patients and amounted to SMD -0.03; 95% CI, -0.09-0.02, -value 0.533 and SMD-0.29; 95% CI, -0.47- -0.12; = 0.001, respectively. For TNF, however, the difference was statistically insignificant.
DISCUSSION
IL-6, unlike TNF and IL-1β, could be a useful and convenient marker of peripheral inflammation in older adults with various comorbidities.
Topics: Aged; Humans; Aging; Cytokines; Inflammation; Interleukin-1beta; Interleukin-6; Tumor Necrosis Factor-alpha
PubMed: 38495887
DOI: 10.3389/fimmu.2024.1330386 -
The Ocular Surface Oct 2023Aging is a complex biological process that is characterized by low-grade inflammation, called inflammaging. Aging affects multiple organs including eye and lacrimal...
Aging is a complex biological process that is characterized by low-grade inflammation, called inflammaging. Aging affects multiple organs including eye and lacrimal gland. Tumor necrosis factor (TNF) is a pleiotropic cytokine that participates in inflammation, activation of proteases such as cathepsin S, and formation of ectopic lymphoid organs. Using genetic and pharmacological approaches, we investigated the role of TNF in age-related dry eye disease, emphasizing the ocular surface and lacrimal gland inflammation. Our results show the increased protein and mRNA levels of TNF in aged lacrimal glands, accompanied by increased TNF, IL1β, IL-18, CCL5, CXCL1, IL-2, IL-2 receptor alpha (CD25), IFN-γ, IL-12p40, IL-17, and IL-10 proteins in tears of aged mice. Moreover, genetic loss of the Tnf in mice decreased goblet cell loss and the development of ectopic lymphoid structures in the lacrimal gland compared to wild-type mice. This was accompanied by a decrease in cytokine production. Treatment of mice at an early stage of aging (12-14-month-old) with TNF inhibitor tanfanercept eye drops for eight consecutive weeks decreased cytokine levels in tears, improved goblet cell density, and decreased the marginal zone B cell frequency in the lacrimal gland compared to vehicle-treated animals. Our studies indicate that modulation of TNF during aging could be a novel strategy for age-related dry eye disease.
Topics: Animals; Mice; Cytokines; Dry Eye Syndromes; Lacrimal Apparatus; Tumor Necrosis Factor-alpha; Tears; Inflammation; Disease Models, Animal
PubMed: 37634571
DOI: 10.1016/j.jtos.2023.08.004 -
Frontiers in Immunology 2019
Topics: Animals; Disease Susceptibility; Homeostasis; Humans; Interleukin-1; Multigene Family
PubMed: 31798575
DOI: 10.3389/fimmu.2019.02596 -
Oxidative Medicine and Cellular... 2021As a chronic inflammatory disease, atherosclerosis is a leading cause of morbidity and mortality in most countries. Inflammation is responsible for plaque instability... (Review)
Review
As a chronic inflammatory disease, atherosclerosis is a leading cause of morbidity and mortality in most countries. Inflammation is responsible for plaque instability and the subsequent onset of acute coronary syndrome (ACS), which is one of the leading causes of hospitalization. Therefore, exploring the potential mechanism underlying ACS is of considerable concern, and searching for alternative therapeutic targets is very urgent. Interleukin-37 (IL-37) inhibits the production of proinflammatory chemokines and cytokines and acts as a natural inhibitor of innate and adaptive immunity. Interestingly, our previous study with murine models showed that IL-37 alleviated cardiac remodeling and myocardial ischemia/reperfusion injury. Of note, our clinical study revealed that IL-37 is elevated and plays a beneficial role in patients with ACS. Moreover, dendritic cells (DCs) orchestrate both immunity and tolerance, and tolerogenic DCs (tDCs) are characterized by more secretion of immunosuppressive cytokines. As expected, IL-37-treated DCs are tolerogenic. Hence, we speculate that IL-37- or IL-37-treated DCs is a novel therapeutic possibility for ACS, and the precise mechanism of IL-37 requires further study.
Topics: Acute Coronary Syndrome; Animals; Dendritic Cells; Humans; Interleukin-1; Mice
PubMed: 34471467
DOI: 10.1155/2021/6454177 -
Nature Reviews. Rheumatology Mar 2019
Topics: Adalimumab; Tumor Necrosis Factor-alpha
PubMed: 30728451
DOI: 10.1038/s41584-019-0183-0 -
Acta Biochimica Et Biophysica Sinica Jan 2017Intervertebral disc (IVD) degeneration (IDD) is the most common cause leading to low back pain (LBP), which is a highly prevalent, costly, and crippling condition... (Review)
Review
Intervertebral disc (IVD) degeneration (IDD) is the most common cause leading to low back pain (LBP), which is a highly prevalent, costly, and crippling condition worldwide. Current treatments for IDD are limited to treat the symptoms and do not target the pathophysiology. Tumor necrosis factor-α (TNF-α) is one of the most potent pro-inflammatory cytokines and signals through its receptors TNFR1 and TNFR2. TNF-α is highly expressed in degenerative IVD tissues, and it is deeply involved in multiple pathological processes of disc degeneration, including matrix destruction, inflammatory responses, apoptosis, autophagy, and cell proliferation. Importantly, anti-TNF-α therapy has shown promise for mitigating disc degeneration and relieving LBP. In this review, following a brief description of TNF-α signal transduction, we mainly focus on the expression pattern and roles of TNF-α in IDD, and summarize the emerging progress regarding its inhibition as a promising biological therapeutic approach to disc degeneration and associated LBP. A better understanding will help to develop novel TNF-α-centered therapeutic interventions for degenerative disc disease.
Topics: Humans; Intervertebral Disc Degeneration; Protein Conformation; Signal Transduction; Tumor Necrosis Factor-alpha
PubMed: 27864283
DOI: 10.1093/abbs/gmw112 -
American Journal of Respiratory Cell... Apr 2023
Topics: Interleukin-1beta; Signal Transduction; Fibroblasts; Lung
PubMed: 36893326
DOI: 10.1165/rcmb.2023-0008ED -
Minerva Anestesiologica Jul 2019
Topics: Cardiac Surgical Procedures; Cardiopulmonary Bypass; Hemadsorption; Interleukin-8; Tumor Necrosis Factor-alpha
PubMed: 30762328
DOI: 10.23736/S0375-9393.19.13516-X -
Current Protocols in Protein Science Nov 2014Purification of human IL-1β is used in this unit as an example of the preparation of a soluble protein from E. coli. Bacteria containing IL-1β are lysed, and IL-1 β... (Review)
Review
Purification of human IL-1β is used in this unit as an example of the preparation of a soluble protein from E. coli. Bacteria containing IL-1β are lysed, and IL-1 β in the resulting supernatant is purified by anion-exchange chromatography, salt precipitation, and cation-exchange chromatography, and then concentrated. Finally, the IL-1 β protein is applied to a gel-filtration column to separate it from remaining higher- and lower-molecular-weight contaminants, the purified protein is stored frozen or is lyophilized. The purification protocol described is typical for a protein that is expressed in fairly high abundance (i.e., >5% total protein) and accumulates in a soluble state. In addition, the purification procedure serves as an example of how to use classical protein purifications methods, which may also be used in conjunction with the affinity-based methods now more commonly used.
Topics: Escherichia coli; Humans; Interleukin-1beta; Recombinant Proteins; Solubility
PubMed: 25367009
DOI: 10.1002/0471140864.ps0602s78 -
The Journal of Allergy and Clinical... Aug 2018
Topics: Interleukin-17; Tumor Necrosis Factor alpha-Induced Protein 3; Tumor Necrosis Factor-alpha
PubMed: 29859964
DOI: 10.1016/j.jaci.2018.05.020