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Mechanisms of Ageing and Development Apr 2022Nervous system maladaptation is linked to the loss of maximal strength and motor control with aging. Motor unit discharge rates are a critical determinant of force... (Meta-Analysis)
Meta-Analysis Review
Nervous system maladaptation is linked to the loss of maximal strength and motor control with aging. Motor unit discharge rates are a critical determinant of force production; thus, lower discharge rates could be a mechanism underpinning maximal strength and motor control losses during aging. This meta-analysis summarized the findings of studies comparing motor unit discharge rates between young and older adults, and examined the effects of the selected muscle and contraction intensity on the magnitude of discharge rate difference between these two groups. Estimates from 29 studies, across a range of muscles and contraction intensities, were combined in a multilevel meta-analysis, to investigate whether discharge rates differed between young and older adults. Motor unit discharge rates were higher in younger than older adults, with a pooled standardized mean difference (SMD) of 0.66 (95%CI= 0.29-1.04). Contraction intensity had a significant effect on the pooled SMD, with a 1% increase in intensity associated with a 0.009 (95%CI= 0.003-0.015) change in the pooled SMD. These findings suggest that reductions in motor unit discharge rates, especially at higher contraction intensities, may be an important mechanism underpinning age-related losses in maximal force production.
Topics: Aged; Aging; Humans; Isometric Contraction; Motor Neurons; Muscle Contraction; Muscle, Skeletal; Patient Discharge
PubMed: 35218849
DOI: 10.1016/j.mad.2022.111647 -
Experimental Physiology Oct 2021What is the topic of this review? Physiological complexity in muscle force and torque fluctuations, specifically the quantification of complexity, how neuromuscular... (Review)
Review
NEW FINDINGS
What is the topic of this review? Physiological complexity in muscle force and torque fluctuations, specifically the quantification of complexity, how neuromuscular complexityis altered by perturbations and the potential mechanism underlying changes in neuromuscular complexity. What advances does it highlight? The necessity to calculate both magnitude- and complexity-based measures for the thorough evaluation of force/torque fluctuations. Also the need for further research on neuromuscular complexity, particularly how it relates to the performance of functional activities (e.g. manual dexterity, balance, locomotion).
ABSTRACT
Physiological time series produce inherently complex fluctuations. In the last 30 years, methods have been developed to characterise these fluctuations, and have revealed that they contain information about the function of the system producing them. Two broad classes of metrics are used: (1) those which quantify the regularity of the signal (e.g. entropy metrics); and (2) those which quantify the fractal properties of the signal (e.g. detrended fluctuation analysis). Using these techniques, it has been demonstrated that ageing results in a loss of complexity in the time series of a multitude of signals, including heart rate, respiration, gait and, crucially, muscle force or torque output. This suggests that as the body ages, physiological systems become less adaptable (i.e. the systems' ability to respond rapidly to a changing external environment is diminished). More recently, it has been shown that neuromuscular fatigue causes a substantial loss of muscle torque complexity, a process that can be observed in a few minutes, rather than the decades it requires for the same system to degrade with ageing. The loss of torque complexity with neuromuscular fatigue appears to occur exclusively above the critical torque (at least for tasks lasting up to 30 min). The loss of torque complexity can be exacerbated with previous exercise of the same limb, and reduced by the administration of caffeine, suggesting both peripheral and central mechanisms contribute to this loss. The mechanisms underpinning the loss of complexity are not known but may be related to altered motor unit behaviour as the muscle fatigues.
Topics: Electromyography; Isometric Contraction; Muscle Contraction; Muscle Fatigue; Muscle, Skeletal; Torque
PubMed: 34472160
DOI: 10.1113/EP089711 -
Scandinavian Journal of Medicine &... May 2023Examine the effects of 42°C hot-water immersion on muscle contraction function and motor unit discharge rates. Voluntary and evoked contraction assessments were...
PURPOSE
Examine the effects of 42°C hot-water immersion on muscle contraction function and motor unit discharge rates. Voluntary and evoked contraction assessments were examined first with a concomitant increase in the core and muscle temperature, and thereafter with increased muscle temperature but cooled core temperature.
METHODS
Fifteen participants (24.9 ± 5.6 years) performed neuromuscular assessments before, after, and ~15-min after either 90-min of 42°C (hot) or 36°C (control) water immersion. Maximal voluntary contraction (MVC) assessment of knee extension was performed along with surface electromyography (sEMG) (vastus lateralis and medialis [VL, VM]) and voluntary activation level (VAL). Resting evoked twitch was elicited for peak torque and time to peak torque analysis. In addition, the VL and VM motor unit discharge rates (MUDR) were measured.
RESULTS
After hot-water immersion (core temperature ↑1°C; muscle temperature ↑2.4°C), MVC torque and VAL decreased (p < 0.05). The sEMG (VL and VM) and peak twitch torque did not change (p > 0.05), while time to peak torque decreased (p = 0.007). The VL and VM MUDR decreased, showing a time effect, after both water immersion conditions (36 and 42°C) (p > 0.001). Fifteen minutes after the hot-water immersion (core temperature at baseline; muscle temperature ↑1.4°C), MVC torque returned to baseline, but VAL remained lower. The sEMG (VL and VM) remained unchanged. Peak twitch torque increased (p < 0.002) and time to peak torque remained lower (p = 0.028). The MUDR remained lower after both water immersion conditions (p < 0.05).
CONCLUSION
Increased core temperature evoked by 42°C hot-water immersion decreases MVC torque and VAL. However, a passive increase in muscle temperature improved evoked muscle contractile function (i.e., time to peak torque [after] and peak twitch torque [~15 min after]). Moreover, a passive increase in muscle temperature reduced the required MUDR to attain the same torque.
Topics: Humans; Temperature; Patient Discharge; Isometric Contraction; Muscle Contraction; Electromyography; Quadriceps Muscle; Muscle, Skeletal; Torque; Hot Temperature
PubMed: 36610040
DOI: 10.1111/sms.14312 -
Methods in Molecular Biology (Clifton,... 2022Only a limited number of large-scale protocols describe the production of mature skeletal muscle fibers from human induced pluripotent stem cells (hiPSCs). Here we...
Only a limited number of large-scale protocols describe the production of mature skeletal muscle fibers from human induced pluripotent stem cells (hiPSCs). Here we describe a novel procedure for simultaneous differentiation of hiPSC into muscle cells and motor neurons, that generates innervated and contractile multinucleated skeletal muscle fibers with sarcomeric organization. Our protocol permits the production of expandable skeletal muscle progenitor cells and mature skeletal muscle fibers that can be used for the exploration of skeletal muscle differentiation for basic research, disease modeling, and drug discovery.
Topics: Cell Differentiation; Cells, Cultured; Humans; Induced Pluripotent Stem Cells; Motor Neurons; Muscle Contraction; Muscle Fibers, Skeletal; Muscle, Skeletal
PubMed: 33368020
DOI: 10.1007/7651_2020_334 -
Experimental Physiology Oct 2021What is the central question of this study? Is there a critical threshold beyond which the loss of muscle contractility is regulated by the level of muscle activation...
NEW FINDINGS
What is the central question of this study? Is there a critical threshold beyond which the loss of muscle contractility is regulated by the level of muscle activation during single-limb exercise of differing intensities and volumes? What is the main finding and its importance? Plateaus in the decline in muscle contractility during single-limb knee extension depended on both exercise volume and contraction intensity. A plateau was only evident with an increase in exercise volume. Muscle activation increased and did not decline despite substantial reductions in contractility. The findings indicate that the decrease in muscle contractility exhibited by resistance-trained men during the performance of submaximal isometric contractions with the knee extensors was not regulated by the level of muscle activation.
ABSTRACT
Our study examined the influence of contraction intensity and exercise volume on changes in muscle contractility and activation of the knee extensor muscles. Maximal voluntary torque (MVT) and rate of change in torque, surface electromyograms, voluntary activation, V-waves and quadriceps resting twitch measures were assessed in 10 resistance-trained men during two experimental sessions. Each session began with an initial baseline series of contractions at a fixed intensity of 40% or 80% MVT. The 40%-only session continued with five contractions to task failure at 40% MVT. The 80% session continued with five contractions to failure each at 80%, 60% and 40% MVT. Greater reductions in MVT were observed during the baseline contractions of the 40%-only session compared with the 80% session at each matched-volume time point (P < 0.05), with similar changes in twitch values (P < 0.001). MVT and twitch values plateaued at each intensity during the 80% session and were significantly different across intensities: 80% > 60% > 40% (P < 0.001). There were no differences for measures during the five contractions at 40% MVT performed on the different days, despite a greater volume of exercise performed prior to the 40% MVT during the 80% session. At each contraction intensity, a plateau in contractility loss was observed as more contractions were performed. We found that initial increases in muscle activation were maintained in the presence of increases in exercise volume and, in contrast to the critical-threshold hypothesis, did not decline in parallel with reductions in muscle contractility.
Topics: Electromyography; Humans; Isometric Contraction; Knee; Male; Muscle Contraction; Muscle Fatigue; Muscle, Skeletal; Quadriceps Muscle; Torque
PubMed: 34411379
DOI: 10.1113/EP089788 -
Experimental Physiology Dec 2021What is the central question of this study? Is the degree of in human muscle affected by different shortening velocities, or contraction type? What are the main findings...
NEW FINDINGS
What is the central question of this study? Is the degree of in human muscle affected by different shortening velocities, or contraction type? What are the main findings and their importance? The PAP response following maximal concentric contractions was independent of velocity. Slow and moderate velocity maximal contractions produced PAP responses like those from maximal isometric contractions when matched for contraction duration. Despite contraction type differences in cross-bridge and Ca kinetics, maximal contractions, regardless of contraction modality, likely generate sufficient Ca to induce maximal PAP.
ABSTRACT
Post-activation potentiation (PAP) is the acute enhancement of contractile properties following a brief (<10 s) high-intensity contraction. Compared with isometric contractions, little is known about the PAP response induced by concentric conditioning contractions (CCs) and the effect of velocity. In the dorsiflexors of 11 participants, twitch responses were measured following 5 s of maximal effort concentric CCs at each of 10, 20 and 50°/s. Concentric PAP responses were compared to a maximal isometric voluntary contraction (MVC) matched for contraction time. Additionally, concentric CCs were compared to isometric CCs matched for mean torque, contraction area and time. The PAP response following maximal concentric CCs was independent of velocity and there was no difference in the PAP response between concentric CCs and an isometric MVC. During maximal contractions, regardless of contraction modality, there is likely sufficient Ca to induce a similar full PAP response, and thus there was no difference between speeds or contraction type. Following concentric CCs there was a significantly larger peak twitch torque than following their isometric torque matches (49-58%), and faster maximal rates of torque development at the three speeds (62-77%). However, these responses are likely related to greater EMG in concentric contractions, 125-129% of isometric maximum compared to 38-54%, and not to contraction modality per se. Thus, PAP responses following maximal concentric CCs are not affected by velocity and responses are not different from an isometric MVC. This indicates maximal CCs of 5 s produce a maximal PAP response independent of contraction type (isometric vs. concentric) or shortening velocity.
Topics: Electromyography; Humans; Isometric Contraction; Muscle Contraction; Muscle, Skeletal; Torque
PubMed: 34569107
DOI: 10.1113/EP089613 -
Physiological Reports Oct 2020The aim of the present study was to compare the mechanical properties of muscles and tendons at high strain rates between sprinters and untrained men. Fifteen sprinters...
The aim of the present study was to compare the mechanical properties of muscles and tendons at high strain rates between sprinters and untrained men. Fifteen sprinters and 18 untrained men participated in this study. Active muscle stiffness of the medial gastrocnemius muscle was calculated according to changes in the estimated muscle force and fascicle length during fast stretching at five different angular velocities (100, 200, 300, 500, and 600 deg·s ) after submaximal isometric contractions. Stiffness and hysteresis of tendon structures were measured during ramp and ballistic contractions. Active muscle stiffness at 500 deg·s (p = .070) and 600 deg·s (p = .041) was greater in sprinters than untrained men, whereas no differences in those at 100, 200, and 300 deg·s were found between the two groups. There were no differences in stiffness or hysteresis of tendon structures measured during ramp and ballistic contractions between the two groups. These results suggest that, for sprinters, greater active muscle stiffness at a high angular velocity is caused by exercising with a high angular velocity that is typical of their training.
Topics: Adult; Biomechanical Phenomena; Exercise; Humans; Isometric Contraction; Male; Muscle Contraction; Muscle, Skeletal; Tendons; Ultrasonography
PubMed: 33038067
DOI: 10.14814/phy2.14583 -
Journal of Applied Biomechanics Dec 2023Muscle fiber conduction velocity (MFCV) can be affected by muscle fiber geometry at different joint angles and during joint movements. This study aimed to investigate...
Muscle fiber conduction velocity (MFCV) can be affected by muscle fiber geometry at different joint angles and during joint movements. This study aimed to investigate MFCV during electrically evoked contraction at different joint angles, during joint movements, and during voluntary contractions. Sixteen healthy young men participated. A stimulation electrode was attached on the innervation zone of the vastus lateralis, and a linear electrode array was attached on the vastus lateralis. Under a static condition, electrically evoked electromyography signals were recorded at knee joint angles set every 15° between 0° and 105°. Under a passive movement condition, signals were recorded during knee extension and flexion passively. Under a voluntary contraction condition, signals were recorded while performing 30% or 60% of maximum voluntary contraction. MFCV was calculated using cross-correlation coefficients. Under the static condition, there were no differences in MFCV among various joint angles. Under the passive movement condition, MFCV was significantly greater during high velocity or shortening. Under the voluntary contraction condition, MFCV was significantly greater during high-intensity voluntary contraction and with a shortened muscle length. Joint angles do not influence MFCV markedly during relaxation, but it is possible to overestimate MFCV during movement or voluntary contraction.
Topics: Male; Humans; Muscle Fibers, Skeletal; Electromyography; Muscle Contraction; Isometric Contraction; Quadriceps Muscle; Muscle, Skeletal
PubMed: 37678832
DOI: 10.1123/jab.2023-0004 -
Journal of Applied Physiology... Oct 2023A potentiating conditioning contraction (CC) has been shown to increase silent period duration, an index of corticospinal inhibition; however, it is unknown if the CC...
A potentiating conditioning contraction (CC) has been shown to increase silent period duration, an index of corticospinal inhibition; however, it is unknown if the CC must induce potentiation for corticospinal inhibition to increase. Ten healthy, young adults (four females) completed this study to assess potentiation and silent period (SP) duration before and after four types of CCs: voluntary and electrically evoked maximal CCs to optimize potentiation, and voluntary and electrically evoked submaximal CCs (∼40% of maximal voluntary force) that induced minimal potentiation. Stimulation was applied to the ulnar nerve to evoke twitches for the assessment of potentiation and to evoke tetanic CCs of the first dorsal interosseous muscle. The SP was elicited by applying transcranial magnetic stimulation to the motor cortex during brief contractions at 25% of maximal voluntary force. Changes to twitch force and SP duration were not different for voluntary and tetanic contractions, so data were pooled. Twitch force increased by 81.2 ± 35.7% ( < 0.001) and 3.2 ± 6.5% ( = 0.039) following maximal and submaximal CCs, respectively. The SP was prolonged following maximal (12.6 ± 6.3%; < 0.001) and submaximal (4.8 ± 4.9%; < 0.001) CCs. Correlations between post-CC twitch force and SP duration were not significant for maximal or submaximal conditions ( = -0.068; = 0.067; ≥ 0.780, respectively). Duration of the SP increased not only following maximal-intensity CCs but also after submaximal-intensity CCs that induced virtually no potentiation (∼3%). Thus, we suggest that corticospinal inhibition is not directly related to mechanisms of muscle potentiation per se, but, rather, the level of muscle contraction likely mediates feedback from large diameter afferents that affect the SP. The transcranial magnetic stimulation-induced silent period reflects a transient state of corticospinal inhibition that is influenced by recent history of muscle activation, which may include an effect of potentiation. We demonstrate that silent period duration increases following both voluntary and electrically evoked maximal and submaximal conditioning contractions, even though the latter intensity produced virtually no muscle potentiation. Feedback from group Ia and Ib muscle afferents is proposed as the cause of the increased corticospinal inhibition.
Topics: Female; Young Adult; Humans; Muscle, Skeletal; Muscle Contraction; Transcranial Magnetic Stimulation; Electric Stimulation; Electromyography; Evoked Potentials, Motor; Muscle Fatigue; Isometric Contraction
PubMed: 37616335
DOI: 10.1152/japplphysiol.00206.2023 -
Biomechanics and Modeling in... Feb 2016Experimental studies show different muscle-tendon complex (MTC) functions (e.g. motor or spring) depending on the muscle fibre-tendon length ratio. Comparing different...
Experimental studies show different muscle-tendon complex (MTC) functions (e.g. motor or spring) depending on the muscle fibre-tendon length ratio. Comparing different MTC of different animals examined experimentally, the extracted MTC functions are biased by, for example, MTC-specific pennation angle and fibre-type distribution or divergent experimental protocols (e.g. influence of temperature or stimulation on MTC force). Thus, a thorough understanding of variation of these inner muscle fibre-tendon length ratios on MTC function is difficult. In this study, we used a hill-type muscle model to simulate MTC. The model consists of a contractile element (CE) simulating muscle fibres, a serial element (SE) as a model for tendon, and a parallel elastic element (PEE) modelling tissue in parallel to the muscle fibres. The simulation examines the impact of length variations of these components on contraction dynamics and MTC function. Ensuring a constant overall length of the MTC by L(MTC) = L(SE) + L(CE), the SE rest length was varied over a broad physiological range from 0.1 to 0.9 MTC length. Five different MTC functions were investigated by simulating typical physiological experiments: the stabilising function with isometric contractions, the motor function with contractions against a weight, the capability of acceleration with contractions against a small inertial mass, the braking function by decelerating a mass, and the spring function with stretch-shortening cycles. The ratio of SE and CE mainly determines the MTC function. MTC with comparably short tendon generates high force and maximal shortening velocity and is able to produce maximal work and power. MTC with long tendon is suitable to store and release a maximum amount of energy. Variation of muscle fibre-tendon ratio yielded two peaks for MTC's force response for short and long SE lengths. Further, maximum work storage capacity of the SE is at long relL(SE,0). Impact of fibre-tendon length ratio on MTC functions will be discussed. Considering a constant set of MTC parameters, quantitative changes in MTC performance (work, stiffness, force, energy storage, dissipation) depending on varying muscle fibre-tendon length ratio were provided, which enables classification and grading of different MTC designs.
Topics: Acceleration; Biomechanical Phenomena; Computer Simulation; Gravitation; Humans; Isometric Contraction; Muscle Contraction; Muscle Fibers, Skeletal; Tendons; Time Factors
PubMed: 26038176
DOI: 10.1007/s10237-015-0688-7