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Cell Jun 2019Xenograft cell transplantation into immunodeficient mice has become the gold standard for assessing pre-clinical efficacy of cancer drugs, yet direct visualization of...
Xenograft cell transplantation into immunodeficient mice has become the gold standard for assessing pre-clinical efficacy of cancer drugs, yet direct visualization of single-cell phenotypes is difficult. Here, we report an optically-clear prkdc, il2rga zebrafish that lacks adaptive and natural killer immune cells, can engraft a wide array of human cancers at 37°C, and permits the dynamic visualization of single engrafted cells. For example, photoconversion cell-lineage tracing identified migratory and proliferative cell states in human rhabdomyosarcoma, a pediatric cancer of muscle. Additional experiments identified the preclinical efficacy of combination olaparib PARP inhibitor and temozolomide DNA-damaging agent as an effective therapy for rhabdomyosarcoma and visualized therapeutic responses using a four-color FUCCI cell-cycle fluorescent reporter. These experiments identified that combination treatment arrested rhabdomyosarcoma cells in the G2 cell cycle prior to induction of apoptosis. Finally, patient-derived xenografts could be engrafted into our model, opening new avenues for developing personalized therapeutic approaches in the future.
Topics: Animals; Animals, Genetically Modified; Antineoplastic Combined Chemotherapy Protocols; Female; Heterografts; Humans; K562 Cells; Male; Muscle Neoplasms; Neoplasm Transplantation; Phthalazines; Piperazines; Rhabdomyosarcoma; Temozolomide; Xenograft Model Antitumor Assays; Zebrafish
PubMed: 31031007
DOI: 10.1016/j.cell.2019.04.004 -
Radiologic Clinics of North America Jul 2016Primary lymphoma of bone and soft tissue is rare and almost invariably of B-cell origin. Osseous lymphoma usually reveals aggressive bone destruction and associated soft... (Review)
Review
Primary lymphoma of bone and soft tissue is rare and almost invariably of B-cell origin. Osseous lymphoma usually reveals aggressive bone destruction and associated soft tissue extension. Soft tissue involvement is optimally depicted by MR imaging. Cortical destruction allowing communication between the intraosseous and soft tissue components may be subtle with small striations of extension. Lymphoma of the deep soft tissues usually reveals long cones of intramuscular or intermuscular tumor again best depicted by MR imaging. Cutaneous or subcutaneous lymphoma demonstrates multiple nodules and plaquelike thickening.
Topics: Bone Neoplasms; Diagnosis, Differential; Evidence-Based Medicine; Humans; Lymphoma; Magnetic Resonance Imaging; Muscle Neoplasms; Positron-Emission Tomography; Tomography, X-Ray Computed
PubMed: 27265608
DOI: 10.1016/j.rcl.2016.03.008 -
Ophthalmic Plastic and Reconstructive... 2018
Topics: Adult; Facial Neoplasms; Humans; Leiomyoma; Male; Muscle Neoplasms; Orbit
PubMed: 28151824
DOI: 10.1097/IOP.0000000000000873 -
Joint Bone Spine Nov 2022
Topics: Humans; Multimodal Imaging; Positron-Emission Tomography; Muscles; Muscle Neoplasms; Muscle, Skeletal
PubMed: 35905836
DOI: 10.1016/j.jbspin.2022.105442 -
Mayo Clinic Proceedings Jan 2019
Topics: Biopsy; Diagnosis, Differential; Humans; Lipoma; Muscle Neoplasms; Thigh
PubMed: 30611449
DOI: 10.1016/j.mayocp.2018.11.007 -
RoFo : Fortschritte Auf Dem Gebiete Der... Dec 2020
Topics: Aged; Biopsy; Diagnosis, Differential; Femoral Neoplasms; Femur; Fibrous Dysplasia of Bone; Humans; Magnetic Resonance Imaging; Male; Muscle Neoplasms; Muscle, Skeletal; Myxoma; Neoplasms, Multiple Primary; Syndrome
PubMed: 32516829
DOI: 10.1055/a-1167-8431 -
RoFo : Fortschritte Auf Dem Gebiete Der... Jul 2019
Topics: Aged; Antineoplastic Agents; Breast Neoplasms; Combined Modality Therapy; Female; Humans; Magnetic Resonance Imaging; Mastectomy; Muscle Neoplasms; Muscle, Skeletal; Neoplasm Recurrence, Local; Neoplasm Staging; Nerve Compression Syndromes; Oculomotor Muscles; Optic Nerve; Tomography, X-Ray Computed
PubMed: 30665252
DOI: 10.1055/a-0820-5711 -
Seminars in Musculoskeletal Radiology Aug 2020The role of quantitative magnetic resonance imaging (MRI) and positron emission tomography/computed tomography (PET/CT) techniques continues to grow and evolve in the... (Review)
Review
The role of quantitative magnetic resonance imaging (MRI) and positron emission tomography/computed tomography (PET/CT) techniques continues to grow and evolve in the evaluation of musculoskeletal tumors. In this review we discuss the MRI quantitative techniques of volumetric measurement, chemical shift imaging, diffusion-weighted imaging, elastography, spectroscopy, and dynamic contrast enhancement. We also review quantitative PET techniques in the evaluation of musculoskeletal tumors, as well as virtual surgical planning and three-dimensional printing.
Topics: Bone Neoplasms; Contrast Media; Humans; Image Enhancement; Image Interpretation, Computer-Assisted; Magnetic Resonance Imaging; Muscle Neoplasms; Positron-Emission Tomography
PubMed: 32992370
DOI: 10.1055/s-0040-1708825 -
Thoracic Surgery Clinics May 2024Diaphragm tumors are very rare, with secondary tumors being more common than primary tumors. The most common benign primary tumors include lipomas and cysts, and... (Review)
Review
Diaphragm tumors are very rare, with secondary tumors being more common than primary tumors. The most common benign primary tumors include lipomas and cysts, and malignant primary tumors include rhabdomyosarcoma and leiomyosarcoma. Endometriosis is the most common benign secondary tumor, followed by malignant tumors with localized spread of disease. In addition, widely metastatic disease has been described. Benign lipomas and cysts can be managed conservatively, but more complex or concerning disease typically requires resection. The diaphragm can often be repaired primarily, though any large defect or tension would indicate the need for mesh or an autologous reconstruction.
Topics: Humans; Diaphragm; Muscle Neoplasms
PubMed: 38705667
DOI: 10.1016/j.thorsurg.2024.01.009 -
Seminars in Roentgenology Oct 2017
Topics: Bone Neoplasms; Diagnostic Imaging; Humans; Muscle Neoplasms; Musculoskeletal System
PubMed: 28965539
DOI: 10.1053/j.ro.2017.09.001