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Cureus Apr 2021species are gram-negative, non-lactose fermenting, aerobic bacilli closely related to genus. () is an emergent human pathogen that is difficult to differentiate from...
species are gram-negative, non-lactose fermenting, aerobic bacilli closely related to genus. () is an emergent human pathogen that is difficult to differentiate from other species by conventional methods. It is known to infect immunocompromised hosts, has the propensity for abscess formation, and is known for its multidrug resistance. We describe the case of an 84-year-old woman with a background of primary sclerosing cholangitis who presented with fatigue, fever, and syncope. Blood cultures grew . Magnetic resonance cholangiopancreatography and endoscopic retrograde cholangiopancreatography were consistent with cholangitis. Cultures from the biliary duct confirmed the same microorganism. The patient was successfully treated with minocycline. Although rare, should be considered as a differential diagnosis in patients with biliary and gut pathology, particularly in immunocompromised patients.
PubMed: 34046280
DOI: 10.7759/cureus.14648 -
Marine Pollution Bulletin Feb 2017Contaminated sediment samples were collected from the Eastern Province, Saudi Arabia for isolation of pyrene- and phenanthrene-degrading bacteria by enrichment method....
Contaminated sediment samples were collected from the Eastern Province, Saudi Arabia for isolation of pyrene- and phenanthrene-degrading bacteria by enrichment method. Four isolates were morphologically characterized as Gram-negative rod strains and 16S rRNA sequence analysis revealed the isolates as closely related to Pseudomonas aeruginosa, P. citronellolis, Ochrobactrum intermedium and Cupriavidus taiwanensis. Degradation of the polycyclic aromatic hydrocarbons (PAHs) by the latter three strains was investigated in liquid cultures. Results of concentration reduction analyzed with gas chromatography show that P. citronellolis_LB was efficient in removing phenanthrene, degrading 94% of 100ppm in 15days while O. intermedium_BC1 was more efficient in pyrene-removal, degrading 62% in 2weeks. Furthermore, bacterial growth assessment using optical density and population counts revealed the latter as more suitable for microbial growth analysis in PAH-containing cultures. In conclusion, the isolated bacterial strains could be further developed for efficient use in biodegradation of PAH.
Topics: Bacteria; Biodegradation, Environmental; Polycyclic Aromatic Hydrocarbons; RNA, Ribosomal, 16S; Saudi Arabia
PubMed: 27912917
DOI: 10.1016/j.marpolbul.2016.11.007 -
Microbiology Resource Announcements Apr 2022Brucella intermedia/Ochrobactrum intermedium strain DF13 was isolated from Brazilian soil and is able to degrade 2,4-dichlorophenoxyacetic acid (2,4-D). Here, we report...
Brucella intermedia/Ochrobactrum intermedium strain DF13 was isolated from Brazilian soil and is able to degrade 2,4-dichlorophenoxyacetic acid (2,4-D). Here, we report on its genome sequence, with 4,570,268 bp and a 57.8% G+C content.
PubMed: 35343769
DOI: 10.1128/mra.01105-21 -
Genomics Data Jun 2016Advances in de novo sequencing technologies allow us to track deeper insights into microbial genomes for restructuring events during the course of their evolution inside...
Advances in de novo sequencing technologies allow us to track deeper insights into microbial genomes for restructuring events during the course of their evolution inside and outside the host. Bacterial species belonging to Ochrobactrum genus are being reported as emerging, and opportunistic pathogens in this technology driven era probably due to insertion and deletion of genes. The Ochrobactrum intermedium M86 was isolated in 2005 from a case of non-ulcer dyspeptic human stomach followed by its first draft genome sequence in 2009. Here we report re-sequencing of O. intermedium M86 laboratory adapted strain in terms of gain and loss of genes. We also attempted for finer scale genome sequence with 10 times more genome coverage than earlier one followed by comparative evaluation on Ion PGM and Illumina MiSeq. Despite their similarities at genomic level, lab-adapted strain mainly lacked genes encoding for transposase protein, insertion elements family, phage tail-proteins that were not detected in original strain on both chromosomes. Interestingly, a 5 kb indel was detected in chromosome 2 that was absent in original strain mapped with phage integrase gene of Rhizobium spp. and may be acquired and integrated through horizontal gene transfer indicating the gene loss and gene gain phenomenon in this genus. Majority of indel fragments did not match with known genes indicating more bioinformatic dissection of this fragment. Additionally we report genes related to antibiotic resistance, heavy metal tolerance in earlier and re-sequenced strain. Though SNPs detected, there did not span urease and flagellar genes. We also conclude that third generation sequencing technologies might be useful for understanding genomic architecture and re-arrangement of genes in the genome due to their ability of larger coverage that can be used to trace evolutionary aspects in microbial system.
PubMed: 27222803
DOI: 10.1016/j.gdata.2016.04.003 -
Access Microbiology 2019Wastewater has become a potential habitat for multi-drug-resistant bacteria. The present study aims to screen for the presence of carbapenem-resistant bacteria in sewage...
Wastewater has become a potential habitat for multi-drug-resistant bacteria. The present study aims to screen for the presence of carbapenem-resistant bacteria in sewage water samples collected from hospital and non-hospital sources. From a total of 19 sewage water samples collected, 100 carbapenem-resistant non-lactose-fermenting Gram-negative bacteria (CR-NF-GNB) were isolated using MacConkey agar cultured with 8 mg l of meropenem. On screening for beta-lactamase resistance genes ( , , , and ), one isolate, , was found to carry the plasmid-borne gene. To the best of our knowledge, we provide the first report of the rare and emerging opportunistic pathogen encoding the OXA-181 gene in its plasmid.
PubMed: 32974517
DOI: 10.1099/acmi.0.000024 -
Journal of Veterinary Research Sep 2023Universally, in microbiological diagnostics the detection of live bacteria is essential. Rapid identification of pathogens enables appropriate remedial measures to be...
INTRODUCTION
Universally, in microbiological diagnostics the detection of live bacteria is essential. Rapid identification of pathogens enables appropriate remedial measures to be taken. The identification of many bacteria simultaneously facilitates the determination of the characteristics of the accompanying microbiota and/or the microbiological complexity of a given environment.
MATERIAL AND METHODS
The effectiveness of the VITEK2 Compact automated microbial identification system and matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry (MALDI-TOF MS), analytical profile index (API) and Remel RapID tests were compared in identification of bacteria isolated from the alpaca gastrointestinal tract.
RESULTS
Most isolates were Gram-positive, such as and and , , and ; ; ; ; ; , , and (the last only isolated manually by API Coryne and the VITEK2 system and (CBC) card). was misidentified by MALDI-TOF MS as (currently ). Gram-positive and Gram-variable were also isolated. Gram-negative , , and ; ; subsp. ; and ; , and ; subsp. ; ; ; ; ; and were also found. The yeasts and were also present.
CONCLUSION
MALDI-TOF MS enabled the identification of pathogens and opportunistic pathogens from the alpaca gut which may represent a high risk to human and animal health.
PubMed: 37786852
DOI: 10.2478/jvetres-2023-0051 -
New Microbes and New Infections Mar 2024is recognized as a rare emerging opportunistic pathogen mostly related with bloodstream infections. In this report, we describe the first clinical case of pneumonia due...
is recognized as a rare emerging opportunistic pathogen mostly related with bloodstream infections. In this report, we describe the first clinical case of pneumonia due to The case involved a 71-year old tetraplegic man hospitalized for vertebral fractures after falling from a ladder.
PubMed: 38357689
DOI: 10.1016/j.nmni.2024.101224 -
Research in Veterinary Science Oct 2014The effects of the lipopolysaccharide (LPS) from Ochrobactrum intermedium in sheep with fasciolosis was reported previously, resulting in lower fecal egg counts and...
The effects of the lipopolysaccharide (LPS) from Ochrobactrum intermedium in sheep with fasciolosis was reported previously, resulting in lower fecal egg counts and fluke burden. In the current study, we analyzed its immunological effects in two groups of sheep, treated (T) and controls (C). Fasciolosis induces a T helper (Th) type-2 response, characterized by IL-4 and IL-10 production; however, at the beginning of the infection, the IFN-γ production predominates (Th type-1 response). Although we did not find differences in IL-4 production or in the expression level of this gene in the hepatic lymph nodes, the expression level of IL-10 was higher (P < 0.05) in the T group at 4 wpi. The IFN-γ production was higher (P < 0.01) at 12 wpi as well as its level of expression at 4 wpi (P < 0.05) in the T group. We found a higher expression level of TGF-β at 4 wpi in the T group (P < 0.05), associated with the previous report of thicker fibrous tracks in a treated group. Immunoglobulin G1, related with a Th type-2 response, was higher (P < 0.01) in the T group at 4 and 12 wpi. In conclusion, the effects of LPS from O. intermedium could have resulted from a predominant Th type-2 immune response.
Topics: Animals; Fasciola hepatica; Fascioliasis; Feces; Immunoglobulin G; Interferon-gamma; Interleukin-10; Interleukin-4; Lipopolysaccharides; Ochrobactrum; Sheep; Sheep Diseases; Th2 Cells; Transforming Growth Factor beta
PubMed: 25151434
DOI: 10.1016/j.rvsc.2014.07.015 -
International Journal of... 2022Inorganic arsenic (As) is a toxic and carcinogenic pollutant that has long-term impacts on environmental quality and human health. plants hyperaccumulate As from soils....
Inorganic arsenic (As) is a toxic and carcinogenic pollutant that has long-term impacts on environmental quality and human health. plants hyperaccumulate As from soils. Soil bacteria are critical for As-uptake by . We examined the use of taxonomically diverse soil bacteria to modulate As speciation in soil and their effect on As-uptake by . Aqueous media inoculated with MK800041, MK344656, MK345459, MK346993 or MK346997 resulted in the oxidation of 5-30% As(III) and a 49-79% reduction of As(V). Soil inoculated with increased extractable As(III) and As(V) from 0.5 and 0.09 in controls to 0.9 and 0.39 mg As kg soil dry weight, respectively. Moreover, and plants inoculated with , , strains, and strains MK344655, MK346994, MK346997, significantly increased As-uptake by 43, 32, 12, 18, 16, and 14%, respectively, compared to controls. The greatest As-accumulation (1.9 ± 0.04 g kg frond Dwt) and bioconcentration factor (16.3 ± 0.35) was achieved in plants inoculated with . Our findings indicate that the tested bacterial strains can increase As-availability in soils, thus enhancing As-accumulation by . , a well-known As-hyperaccumulator, has the remarkable ability to accumulate higher levels of As in their above-ground biomass. The As-tolerant bacteria-plant interactions play a significant role in bioremediation by mediating As-redox and controlling As-availability and uptake by . Our studies indicated that most of the tested bacterial strains isolated from As-impacted soil significantly enhanced As-uptake by . oxidized 20% of As(III) and reduced 50% of As(V), increased As-extraction from soils, and increased As-uptake by 43% greater compared with control. Therefore, these strains associated with can be used in large-scale field applications to remediate As-contaminated soil.
Topics: Arsenic; Bacteria; Biodegradation, Environmental; Pteris; Soil; Soil Pollutants
PubMed: 34334062
DOI: 10.1080/15226514.2021.1951654 -
Tropical Life Sciences Research Apr 2015Endophytic nitrogen fixing bacteria were isolated from the leaves, stems and roots of industrial variety (cv. U-Thong 3; UT3), wild and chewing sugarcane plants grown...
Endophytic nitrogen fixing bacteria were isolated from the leaves, stems and roots of industrial variety (cv. U-Thong 3; UT3), wild and chewing sugarcane plants grown for 6 weeks in nitrogen (N)-free sand. Eighty nine isolates of endophytic bacteria were obtained on N-free agar. An acetylene reduction assay (ARA) detected nitrogenase activity in all 89 isolates. Three isolates from the chewing (C2HL2, C7HL1 and C34MR1) sugarcane and one isolate from the industrial sugarcane (UT3R1) varieties were characterised, and their responses to different yeast extract concentrations were investigated. Three different responses in nitrogenase activity were observed. Isolates C2HL2 and C7HL1 exhibited major increases with the addition of 0.005% yeast extract, C34MR1 exhibited no response, and UT3R1 exhibited a significant decrease in nitrogenase activity with 0.005% yeast extract. In all the isolates, nitrogenase activity decreased with further increase of the yeast extract to 0.05%. The highest nitrogenase activity was observed in isolates C2HL2 and C7HL1, which had 16S rRNA gene sequences that were closely related to Novosphingobium sediminicola and Ochrobactrum intermedium, respectively.
PubMed: 26868592
DOI: No ID Found