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Journal of Texture Studies Aug 2022The use of insects is considered by Food and Agriculture Organization (FAO) as an alternative source of protein for human and animal food in order to reduce the carbon...
The use of insects is considered by Food and Agriculture Organization (FAO) as an alternative source of protein for human and animal food in order to reduce the carbon footprint caused by meat production and to supply the expected population increase with food. In this regard, the aim of this study was to analyze the physicochemical aspects (water activity, protein and fat content, size, optical and mechanical properties) of biscuits prepared with two different insect powders (Tenebrio molitor [TM] and Alphitobius diaperinus [AD], in different percentages [0, 13, 17, 20, and 25%]) based on total weight. In addition, the biscuits formulated with the highest insect powder content were subjected to sensory analysis. The results revealed that all the biscuits formulated with insect powders may be labeled as "high in protein" products following Regulation (EC) No. 1924/2006, although they were not as thick as the control biscuits. The L* and b* coordinates were significantly lower when the insect powders were included, which implied a darkening of the biscuits, which were also harder than the control biscuits, regardless the concentration used. According to the sensory analysis, most panelists perceived the biscuits containing insects as too dark and not crunchy enough and an increase in sweetness might improve their acceptability. Finally, further studies should be carried out to analyze the influence of different structural components and flavorings, such as chocolate, in the formulation of biscuits with insect powders to improve their sensory attributes.
Topics: Animals; Chocolate; Coleoptera; Flour; Humans; Taste; Tenebrio
PubMed: 35560352
DOI: 10.1111/jtxs.12692 -
Environmental Entomology Aug 2023Ornamental plant production in eastern Virginia nurseries have been greatly impacted by Systena frontalis (F.), also known as the red-headed flea beetle. With the advent...
Ornamental plant production in eastern Virginia nurseries have been greatly impacted by Systena frontalis (F.), also known as the red-headed flea beetle. With the advent of S. frontalis as a prevalent pest in the past 2 decades, baseline phenology and behavior are currently understudied within Virginia nurseries. This pest is costly to control due to insecticide expenses and loss of saleable plants. In 2021 and 2022, populations of this insect were monitored at 2 commercial nurseries in eastern Virginia in order to better understand their temporal and spatial population dynamics. Patterns that emerged indicated S. frontalis could have up to 3 generations in eastern Virginia, with peaks of adult abundance in June, late July, and late August to early September. Phenylethyl alcohol was tested as an adult attractant lure, but it was found to be ineffective under nursery conditions. Diel monitoring demonstrated these adults were most active from 1100 to 1500 h. Severity of defoliation at the leaf level increased linearly with increased density of adults, where 5 individuals defoliated up to 4% of any Hydrangea paniculata cv. 'Limelight' leaf in 1 wk under greenhouse conditions. Timing of scouting and insecticide sprays according to the adult activity peaks of the day and across the season may allow reduction in overall insecticide usage.
Topics: Animals; Coleoptera; Insecticides; Virginia; Gardens
PubMed: 37463285
DOI: 10.1093/ee/nvad058 -
Scientific Reports Jul 2020Nature features a plethora of extraordinary photonic architectures that have been optimized through natural evolution in order to more efficiently reflect, absorb or...
Nature features a plethora of extraordinary photonic architectures that have been optimized through natural evolution in order to more efficiently reflect, absorb or scatter light. While numerical optimization is increasingly and successfully used in photonics, it has yet to replicate any of these complex naturally occurring structures. Using evolutionary algorithms inspired by natural evolution and performing particular optimizations (maximize reflection for a given wavelength, for a broad range of wavelength or maximize the scattering of light), we have retrieved the most stereotypical natural photonic structures. Whether those structures are Bragg mirrors, chirped dielectric mirrors or the gratings on top of Morpho butterfly wings, our results indicate how such regular structures might have spontaneously emerged in nature and to which precise optical or fabrication constraints they respond. Comparing algorithms show that recombination between individuals, inspired by sexual reproduction, confers a clear advantage that can be linked to the fact that photonic structures are fundamentally modular: each part of the structure has a role which can be understood almost independently from the rest. Such an in silico evolution also suggests original and elegant solutions to practical problems, as illustrated by the design of counter-intuitive anti-reflective coatings for solar cells.
Topics: Algorithms; Animals; Biological Evolution; Butterflies; Coleoptera; Computational Biology; Computer Simulation; Nanostructures; Photons; Reproduction; Wings, Animal
PubMed: 32694514
DOI: 10.1038/s41598-020-68719-3 -
Pest Management Science Dec 2023Khapra beetle (Trogoderma granarium Everts), one of the most important quarantine pests globally, is capable of causing severe infestation and huge economic loss to...
BACKGROUND
Khapra beetle (Trogoderma granarium Everts), one of the most important quarantine pests globally, is capable of causing severe infestation and huge economic loss to stored grain, and its interception rate has increased in major global trade countries over the past few years. However, difficulties remain in distinguishing this species with similar ones. In order to assist border ports and warehouses in khapra beetle's effective rapid identification as well as pest control at the early stages of monitoring or interception, we herein developed a new and rapid visual detection assay for T. granarium based on recombinase polymerase amplification (RPA) and the CRISPR/Cas12a system.
RESULTS
We designed and selected the first khapra beetle-specific RPA primers and crRNA, and optimized the visualization reaction system (Cas12a/CrRNA = 100 nM/500 nM). With only a 37 °C-heat-source and a blue light torch, RPA and CRISPR/CAS12a-based visualization assays can be completed within 40 min to differentiate between khapra beetle and nine similar Dermestidae species. After DNA extraction using a kit (4-5 h) or a simple method (5 min), the specific amplicons were obtained after a 15 min RPA reaction at 37 °C, followed by a 15 min color reaction under 37 °C in dark conditions using a CRISPR/CAS12a system and a fluorescent probe (5'-FAM/3'-BHQ1 labeled). This method is ingenious to low levels of DNA (10 ng μL ) and meets the sensitivity requirements for detecting a single khapra beetle's egg (≈0.7 mm).
CONCLUSION
Our specificity and sensitivity analysis inferred that the present visualization system is effective to quickly and uniquely detect khapra beetle at room temperature (37 °C), thereby preventing this species before they spread widely. Our study is suitable for being pushed forward in storage pest management, and provides value as a reference for monitoring and identification of other pests. © 2023 Society of Chemical Industry.
Topics: Animals; CRISPR-Cas Systems; Recombinases; Coleoptera; DNA
PubMed: 37605962
DOI: 10.1002/ps.7739 -
Zootaxa Apr 2023We describe the larvae of the diving-beetle genus Sandracottus Sharp, 1882 based on S. femoralis Heller, 1934 and S. mixtus (Blanchard, 1843). We include detailed...
Morphology of the larvae of Sandracottus femoralis Heller, 1934, and S. mixtus (Blanchard, 1843) and phylogenetic comparison with other known Aciliini (Coleoptera: Dytiscidae, Dytiscinae).
We describe the larvae of the diving-beetle genus Sandracottus Sharp, 1882 based on S. femoralis Heller, 1934 and S. mixtus (Blanchard, 1843). We include detailed morphometric and chaetotaxic analyses of the cephalic capsule, head appendages, legs, terminal abdominal segment and urogomphi in order to discover useful characters for distinguishing Sandracottus larvae from those of other known Aciliini (Coleoptera: Dytiscidae, Dytiscinae). A provisional parsimony analysis based on larval characteristics of seven Aciliini species in four genera (Acilius Leach, 1817, Graphoderus Dejean, 1833, Sandracottus, Thermonectus Dejean, 1833) was conducted using the program TNT. A clade grouping Eretini and Aciliini stands out as the most strongly supported in this analysis. The tribe Aciliini finds itself monophyletic with also very good support. Our analysis, however, failed to depict any clear phylogenetic relationships within the Aciliini although each of the four genera studied emerged from our study as morphologically distinct.
Topics: Animals; Coleoptera; Larva; Phylogeny; Records
PubMed: 37044983
DOI: 10.11646/zootaxa.5263.3.1 -
Zootaxa Apr 2022Larvae and pupae of Pseudopyrochroa facialis were collected beneath bark and in coarse, decaying woody debris of a standing, dead Cyclobalanopsis glauca tree in Guiyang...
Descriptions of the mature larva and pupa of Pseudopyrochroa facialis (Fairmaire) from Southwest China (Coleoptera: Pyrochroidae: Pyrochroinae), with redescriptions of adult stages and notes on natural history.
Larvae and pupae of Pseudopyrochroa facialis were collected beneath bark and in coarse, decaying woody debris of a standing, dead Cyclobalanopsis glauca tree in Guiyang City, Guizhou Province, China. The species association was confirmed by rearing both males and females to the adult stage. This represents the first record of a mainland Chinese species of Pseudopyrochroa to be reared in order to establish species association and identity. The mature larva and pupa are described and illustrated along with descriptions and illustrations of reared and additional adult males and females. Observations relating to the larval/pupal macro- and microhabitats and natural history are provided.
Topics: Animals; China; Coleoptera; Female; Larva; Male; Pupa; Trees
PubMed: 36101212
DOI: 10.11646/zootaxa.5125.3.4 -
Molecular Phylogenetics and Evolution Oct 2021Marine rockpools are isolated patches of habitat in the supratidal environment (the so-called splash zone), at the transition between sea and land, found along the rocky...
Marine rockpools are isolated patches of habitat in the supratidal environment (the so-called splash zone), at the transition between sea and land, found along the rocky shores worldwide and characterized by harsh conditions for life. Nonetheless, few specialized invertebrates successfully colonized this peculiar environment. Among them several members of the water beetles Ochthebius Leach, 1815, subgenus Cobalius Rey (1886), which are found almost exclusively in supratidal and upper-most intertidal marine rockpools from the eastern Atlantic Ocean (Cape Verde, Canary Islands and Morocco, Madeira, Azores) throughout the whole Mediterranean basin. The subgenus Cobalius before 2020 was considered to include ten valid species, based on morphological differences. In late 2020, four additional new species were described. However, recent molecular phylogenetic studies have uncovered further cryptic diversity suggesting the presence of multiple undetected species within this group, highlighting that the species boundaries remain unclear and systematics and taxonomy are in need of revision. In this study we provide a molecular phylogeny based on DNA sequence data from mitochondrial and nuclear genes obtained from ten described species belonging to the subgenus Cobalius, and extensive taxon sampling, in order to better understand the phylogenetic relationships within this genus and to infer the biogeographic processes behind its diversification. We also used a molecular clock to define a time window for diversification of distinct clades within the subgenus, and explore aspects of its evolutionary history. Finally, we used three species delimitation methods (PTP, GMYC and ABGD) to clarify taxonomy and validate species boundaries. Our phylogenetic and biogeographic results identified sixteen independent lineages grouped in four main clades and the possible origin of Cobalius was estimated to be in the Early Miocene (~22 Mya) in W Mediterranean area. Moreover, species delimitation methods suggest there are between 16 and 24 putative species, most of them diverged during the Late Miocene, Pliocene and Pleistocene (6.0-0.11 Mya).
Topics: Animals; Base Sequence; Bayes Theorem; Coleoptera; DNA, Mitochondrial; Ecosystem; Phylogeny; Portugal
PubMed: 34224850
DOI: 10.1016/j.ympev.2021.107243 -
Comparative Biochemistry and... Mar 2019The Malpighian tubules in insects play an essential role in osmoregulation, through the transport of ions during excretion, whereas the fat body is usually associated... (Comparative Study)
Comparative Study
The Malpighian tubules in insects play an essential role in osmoregulation, through the transport of ions during excretion, whereas the fat body is usually associated with the intermediary metabolism. The tubules also are involved in excretion of organic solutes and xenobiotics. However, with the exception of a preliminary transcriptional survey of the Zophobas morio (Tenebrionidae) larval tubules, there are no detailed transcriptional analysis of this organ in Coleoptera. A luciferase-like enzyme that displays weak luminescence activity in the presence of firefly D-luciferin and ATP was cloned from the tubules of Z. morio larvae. In order to better understand the molecular physiology of Malpighian tubules and fat body in Coleoptera larvae, and to investigate the occurrence and functions of AMP-CoA ligases in these tissues, we performed a comparative transcriptional analysis of these tissues using Z. morio giant-mealworms. As expected, the tubules displayed organic and inorganic transporters, xenobiotic metabolism enzymes, V-ATPases, channels, and pumps. The fat body showed proteins that are synthesized in this tissue and secreted to the hemolymph, as well as enzymes involved in lipid and carbohydrate metabolism. These tissues are also involved in common pathways, such as nitrogen metabolism to degradation/excretion, eye pigments biosynthesis, immunity, and detoxification. The presence of coumarate-CoA ligase-like enzymes in these tissues suggest their involvement in the degradation of coumaric acid derivatives obtained from the diet, or alternatively, in the biosynthesis of compounds structurally related to coumaric acids such as eye pigments. Our results confirm to the physiological versatility of tubules and fat body in larval Coleoptera.
Topics: Amino Acid Sequence; Animals; Coleoptera; Fat Body; Gene Expression Profiling; Genes, Insect; Insect Proteins; Larva; Malpighian Tubules; RNA, Messenger; Sequence Homology, Amino Acid; Transcription, Genetic
PubMed: 30463043
DOI: 10.1016/j.cbd.2018.11.007 -
Bulletin of Entomological Research Dec 2018It is difficult to control Holotrichia parallela Motschulsky with chemical insecticides due to the larvae's soil-living habit, thus the pest has caused great economic...
It is difficult to control Holotrichia parallela Motschulsky with chemical insecticides due to the larvae's soil-living habit, thus the pest has caused great economic losses in agriculture. In addition, uridine diphosphate-glycosyltransferases (UGTs) catalyze the glycosylation process of a variety of small lipophilic molecules with sugars to produce water-soluble glycosides, and play multiple roles in detoxification, endobiotic modulation, and sequestration in an insect. Some UGTs were found specifically expressed in antennae of Drosophila melanogaster and Spodoptera littoralis, and glucurono-conjugated odorants could not elicit any olfactory signals, suggesting that the UGTs may play roles in odorant inactivation by biotransformation. In the current study, we performed a genome-wide analysis of the candidate UGT family in the dark black chafer, H. parallela. Based on a UGT gene signature and the similarity of these genes to UGT homologs from other organisms, 20 putative H. parallela UGT genes were identified. Bioinformatics analysis was used to predict sequence and structural features of H. parallela UGT proteins, and revealed important domains and residues involved in sugar donor binding and catalysis by comparison with human UGT2B7. Phylogenetic analysis of these 20 UGT protein sequences revealed eight major groups, including both order-specific and conserved groups, which are common to more than one order. Of these 20 UGT genes, HparUGT1265-1, HparUGT3119, and HparUGT8312 were highly (>100-fold change) expressed in antennae, suggesting a possible role in olfactory tissue, and most likely in odorant inactivation and olfactory processing. The remaining UGT genes were expressed in all tissues (head, thorax, abdomen, leg, and wing), indicating that these UGTs likely have different biological functions. This study provides the fundamental basis for determining the function of UGTs in a highly specialized olfactory organ, the H. parallela antenna.
Topics: Amino Acid Sequence; Animals; Arthropod Antennae; Coleoptera; Female; Gene Expression Profiling; Glycosyltransferases; Insect Proteins; Male; Phylogeny; Sequence Alignment; Uridine Diphosphate
PubMed: 29397056
DOI: 10.1017/S0007485318000068 -
The Science of the Total Environment Dec 2023Anthropogenic contamination from coal-fired power plants and nuclear reactors is a pervasive issue impacting ecosystems across the globe. As a result, it is critical...
Anthropogenic contamination from coal-fired power plants and nuclear reactors is a pervasive issue impacting ecosystems across the globe. As a result, it is critical that studies continue to assess the accumulation and effects of trace elements and radionuclides in a diversity of biota. In particular, bioindicator species are a powerful tool for risk assessment of chemically contaminated habitats. Using inductively coupled plasma mass spectrometry (ICP-MS) and auto-gamma counting, we analyzed trace element and radiocesium contaminant concentrations in Scarabaeidae and Silphidae beetles (Order: Coleoptera), important taxa in decomposition and nutrient cycling, at contaminated and reference sites on the Savannah River Site, South Carolina, U.S. Our results revealed variability in trace element concentrations between Scarabaeidae and Silphidae beetles at uncontaminated and contaminated sites. Compared to Scarabaeidae, Silphidae had higher levels of chromium (Cr), copper (Cu), iron (Fe), manganese (Mn), nickel (Ni), and zinc (Zn). Unexpectedly, concentrations of Cr, Cu, and Ni were higher in both taxa at the uncontaminated sites. Scarabaeidae and Silphidae beetles at the coal combustion waste site consistently had high concentrations of arsenic (As), and Scarabaeidae had high concentrations of selenium (Se). Of the 50 beetles analyzed for radiocesium levels, two had elevated radioactivity concentrations, both of which were from a site contaminated with radionuclides. Our results suggest carrion beetles may be particularly sensitive to bioaccumulation of contaminants due to their trophic position and role in decomposition, and thus are useful sentinels of trace element and radionuclide contamination.
Topics: Animals; Trace Elements; Coleoptera; Ecosystem; Coal; Bioaccumulation; Nickel; Chromium; Radioisotopes
PubMed: 37678529
DOI: 10.1016/j.scitotenv.2023.166821