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Environmental Monitoring and Assessment Jan 2023Water quality monitoring allows communities to achieve sustainable management of water resources, which is crucial for life-supporting processes. Water quality is...
Water quality monitoring allows communities to achieve sustainable management of water resources, which is crucial for life-supporting processes. Water quality is determined by measuring chemical, physical, and biological parameters, requiring sophisticated meters and trained specialists to perform the measurement. However, in low-income communities, water quality is determined by using human senses-smell, color, and taste-since meter acquisition is limited by costs and most people do not know how to monitor water quality. Therefore, accessible technology is necessary to empower communities to have a sustainable lifestyle. In this paper, we present the design and implementation of PortAqua, a 2-parameter water quality meter (WQM), to promote training on water quality measurement. Using basic electronic components, PortAqua is capable of measuring pH with an error of 0.4, and conductivity with an error of 33% at 85 µS cm, and 8.7% at 1413 µS cm. To demonstrate its preliminary effectiveness as a WQM and its science communication capabilities, the meter has been used in a hands-on workshop with undergraduate and graduate students. During the workshop, attendees participated in a short lecture about water quality measurement techniques and local regulations. Then, they collected water samples from a local source, measured the samples using PortAqua, and discussed the results based on the concepts and regulations. The workshop's effectiveness was evaluated through pre- and post-assessments which revealed increased knowledge of water quality regulations, measurement, and parameters at the end of the activity.
Topics: Humans; Water Quality; Environmental Monitoring; Students; Communication
PubMed: 36705766
DOI: 10.1007/s10661-022-10804-3 -
NeuroImage Jul 2019Rhythm perception refers to the mental interpretation of rhythm by a listener. Musical rhythm perception typically involves two steps: beat extraction and metrical...
Rhythm perception refers to the mental interpretation of rhythm by a listener. Musical rhythm perception typically involves two steps: beat extraction and metrical structure assignment (meter perception). The entrainment theories propose that different neuronal oscillations entrain to different levels of metrical structure in the rhythm (e.g., beat and meter) and thereby form a representation of the rhythm in the mind. Thus, neuronal populations that entrain to beat and meter should theoretically be different. However, although entrainment theories have been supported by many studies, the neuronal populations that entrain to beat and meter remain largely unknown. In this study, we used a paradigm to induce neuronal entrainment to beat and meter and obtained images of the neuronal populations with an electroencephalogram functional magnetic resonance imaging (EEG-fMRI) fusion method. We observed that some neuronal populations, including the bilateral putamen, bilateral caudate, left thalamus, and supplementary motor area (SMA), entrain to both beat and meter. We also observed that the bilateral putamen entrains more to meter and the SMA entrains more to beat. Our results suggest that the bilateral putamen plays an important role in meter perception.
Topics: Acoustic Stimulation; Auditory Perception; Brain; Electroencephalography; Female; Humans; Magnetic Resonance Imaging; Male; Music; Neurons; Young Adult
PubMed: 30914384
DOI: 10.1016/j.neuroimage.2019.03.039 -
BMC Pulmonary Medicine Dec 2019There is little information about vitamin D (Vit D) deficiency in patients with pulmonary hypertension (PH). The objective of this study was: 1) compare Vit D levels...
BACKGROUND
There is little information about vitamin D (Vit D) deficiency in patients with pulmonary hypertension (PH). The objective of this study was: 1) compare Vit D levels between patients with PH, left ventricular failure (LVF) and healthy subjects (HS); 2) correlate, in patients with PH, Vit D levels with prognosis-related variables, such as the 6-min walk test (6MWT).
METHODS
Vitamin D levels were measured in a cross-sectional study in 126 patients from one of three groups: patients with PH (n = 53), patients with LVF (n = 42) and healthy subjects (n = 31). In all groups, 8-h fasting blood samples were obtained in the morning. In the PH and the LVF group, functional class (WHO criteria), metres covered in the 6MWT and echocardiographic parameters were analysed. In the PH group, plasma N terminal pro B type natriuretic peptide (NT-proBNP) level was analysed and a complete haemodynamic evaluation by right heart catheterisation was made.
RESULTS
Mean Vit D levels were lower in PH than in both other groups (ng/ml, mean ± SD): PH 19.25 ± 10, LVF 25.68 ± 12, HS 28.8 ± 12 (PH vs LVF p = 0.017, PH vs HS p = 0.001 and HS vs LVF p = 0.46). Vit D deficiency prevalence was higher in PH as compared to the other groups (PH 53.8%, LVF 45.2%, HS 25%, p = 0.01). Patients with PH in functional class (FC; WHO criteria) III-IV had higher Vit D deficiency prevalence than those in FC I-II (86.7% vs 40.5%, p = 0.003). There was a significant linear correlation between the 6MWT and Vit D levels in PH (p < 0.01), but not in LVF (p = 0.69).
CONCLUSIONS
Vit D levels were lower in patients with PH as compared to patients with LVF and HS and correlated directly with 6-min walk distance.
Topics: Adult; Aged; Case-Control Studies; Cross-Sectional Studies; Echocardiography; Female; Heart Failure; Humans; Hypertension, Pulmonary; Male; Middle Aged; Natriuretic Peptide, Brain; Peptide Fragments; Prevalence; Prognosis; Ventricular Dysfunction, Left; Vitamin D; Vitamin D Deficiency; Walk Test; Young Adult
PubMed: 31864342
DOI: 10.1186/s12890-019-1011-7 -
The Journal of Sports Medicine and... Sep 2018The walking speed maintained during a moderate 1-km treadmill walk (1k-TWT) has been demonstrated to be a valid tool for estimating peak oxygen uptake (VO2peak), and to...
BACKGROUND
The walking speed maintained during a moderate 1-km treadmill walk (1k-TWT) has been demonstrated to be a valid tool for estimating peak oxygen uptake (VO2peak), and to be inversely related to long-term survival and hospitalization in outpatients with cardiovascular disease (CVD). We aimed to examine whether 500-meters and 1-k moderate treadmill-walking tests equally estimate VO2peak in male outpatients with CVD.
METHODS
One hundred forty-two clinically stable male outpatients with CVD, aged 34-92 years, referred to an exercise-based secondary prevention program, performed a moderate and perceptually-regulated (11-13/20 on the Borg Scale) 1k-TWT. Age, height, weight, time to walk 500-meter and the entire 1000-meter, and the corresponding heart rates were entered into validated equations to estimate VO2peak.
RESULTS
VO2peak estimated from the 500-meters test was not different from that estimated from the 1k test (25.2±5.1 vs. 25.1±5.2 mL/kg/min). The correlation coefficient between the two was 0.98. The slope and the intercept of the relationship between the 500-meter and 1k tests were not different from the line of identity. Bland-Altman analysis demonstrated that 96% of the data points were within two standard deviations (from -1.9 to 1.7 mL/kg/min).
CONCLUSIONS
The 500-meter treadmill-walking test is a reliable method for estimating VO2peak in stable male outpatients with CVD. A shorter version of the test, 500-meter, provides similar information as that from the original 1k test, but is more time efficient. These findings have practical implications in the context of transitioning patients from clinically based and supervised programs to fitness facilities or self-guided exercise programs.
Topics: Aged; Cardiac Rehabilitation; Cardiorespiratory Fitness; Exercise Test; Heart Rate; Humans; Male; Middle Aged; Oxygen Consumption; Walking
PubMed: 28967238
DOI: 10.23736/S0022-4707.17.07525-9 -
Veterinary Clinical Pathology Jun 2018Urine pH is an integral part of a complete urinalysis, and is commonly measured in veterinary practice using semiquantitative reagent strips. (Comparative Study)
Comparative Study
BACKGROUND
Urine pH is an integral part of a complete urinalysis, and is commonly measured in veterinary practice using semiquantitative reagent strips.
OBJECTIVE
The aim of this study was to compare the urine pH of dogs and sheep, using visual interpretation of dipstick reactions, and using a pH-meter as the reference method. Agreement between the 2 methods was also assessed. An additional objective was to compare the urine pH before and after centrifugation.
METHODS
A total of 50 voided urine samples from sheep and 52 from dogs were collected into sterile containers. For pH measurements, 2 methods were used, a pH-meter and urine dipstick reagent pads. Measurements were performed using urine samples before (whole urine) and after centrifugation (urine supernatant). For comparison of the 2 methods, Passing and Bablok regression analysis and Bland-Altman plots were used.
RESULTS
The equation created to assess agreement between the 2 methods in dogs showed a constant bias at -0.14 and a positive proportional bias at 0.98. From a clinical standpoint, total bias was below and above the maximum acceptable bias in sheep and dogs, respectively. Clinically acceptable bias was also found using centrifuged urine samples in sheep, but the urine pH values before and after centrifugation were nearly identical in dogs.
CONCLUSION
Urine dipstick reagent pads and pH-meters can be used interchangeably to determine urine pH in sheep without needing centrifugation. In contrast, pH-meters provide more accurate pH measurements than urine dipstick pads in canine urine, which is not improved by centrifugation.
Topics: Animals; Dogs; Hydrogen-Ion Concentration; Reagent Strips; Sheep; Urinalysis
PubMed: 29406602
DOI: 10.1111/vcp.12581 -
Reproduction in Domestic Animals =... Oct 2017The aim of the present research is to study the effect of pH values on the sperm rheotaxis properties. Semen collected from bulls was diluted with SOF medium (1:10). pH... (Comparative Study)
Comparative Study
The aim of the present research is to study the effect of pH values on the sperm rheotaxis properties. Semen collected from bulls was diluted with SOF medium (1:10). pH of the medium was adjusted using a digital pH meter to the following pH values: 6.0, 6.2, 6.4, 6.4, 6.8, 7.0. All kinetic parameters of sperm (n = 3,385) were determined through a computer-assisted sperm analysis (CASA) system using microfluidic devices with controlled flow velocity. The following parameters were determined: total motility (TM%), positive rheotaxis (PR%), straightline velocity (VSL, μm/s), average path velocity (VAP, μm/s), linearity (LIN, as VSL/VCL, %), beat cross-frequency (BCF, Hz) and curvilinear velocity (VCL, μm/s). Nitric oxide, calcium and potassium were estimated in semen at different pH values. To confirm the effect of nitric oxide and K , we used sodium nitroprusside (an NO donor) and KCL as (a K donor) to see their effect on sperm PR%. The results showed no difference in TM% at pH (6-7). The PR% was the lowest at pH 6 and 7. The best parameters for the PR% were at pH 6.4-6.6. The concentration of Ca did not change at different pH values. The mean NO values decreased with the increase of pH; however, the mean values of K increased with the increase of pH. Addition of high concentration of NO and K to the semen media at fixed pH level had a negative effect on TM% and PR%. In conclusion, the bull sperm had the best rheotaxis properties at pH 6.4-6.6 and sensitive to the change of seminal NO and K .
Topics: Animals; Calcium; Cattle; Hydrogen-Ion Concentration; Male; Microfluidics; Nitric Oxide; Potassium; Semen Analysis; Sperm Motility; Spermatozoa
PubMed: 28512759
DOI: 10.1111/rda.12979 -
Nanomaterials (Basel, Switzerland) Dec 2021For more than 50% of multiparous cows, it is difficult to adapt to the sudden increase in calcium demand for milk production, which is highly likely to cause...
For more than 50% of multiparous cows, it is difficult to adapt to the sudden increase in calcium demand for milk production, which is highly likely to cause hypocalcemia. An electrochemical biosensor is a portable and efficient method to sense Ca concentrations, but biomaterial is easily affected by the pH of the analyte solution. Here, an electrochemical biosensor was fabricated using a glassy carbon electrode (GCE) and single-walled carbon nanotube (SWNT), which amplified the impedance signal by changing the structure and length of the DNAzyme. Aiming at the interference of the pH, the electrochemical biosensor (GCE/SWNT/DNAzyme) was coupled with a pH meter to form an electrochemical device. It was used to collect data at different Ca concentrations and pH values, and then was processed using different mathematical models, of which GPR showed higher detecting accuracy. After optimizing the detecting parameters, the electrochemical device could determine the Ca concentration ranging from 5 μM to 25 mM, with a detection limit of 4.2 μM at pH values ranging from 4.0 to 7.5. Finally, the electrochemical device was used to determine the Ca concentrations in different blood and milk samples, which can overcome the influence of the pH.
PubMed: 35009954
DOI: 10.3390/nano12010004 -
ANZ Journal of Surgery Oct 2017Adequate hepatopetal portal vein blood flow is obligatory to ensure proper liver function after liver transplantation. Large collateral veins as shunts impair portal... (Review)
Review
Adequate hepatopetal portal vein blood flow is obligatory to ensure proper liver function after liver transplantation. Large collateral veins as shunts impair portal vein flow and even cause hepatofugal blood flow and portal steal syndrome. In particular, splenorenal shunts in liver transplant recipients can lead to allograft dysfunction and possible allograft loss or hepatic encephalopathy. Restoration of portal flow through left renal vein ligation (LRVL) is a treatment option, which is much easier compared to splenectomy, renoportal anastomosis and shunt closure, but bears the risk of moderate and temporary impairment of renal function. In addition, a patent portal vein is mandatory for LRVL. However, although LRVL has been reported to be an effective, safe and easy method to control portacaval shunts and increase hepatopetal flow in some studies, indications and safety are still not clear. In this review, we summarize existing studies on LRVL during liver transplantation.
Topics: Adult; Anastomosis, Surgical; Female; Humans; Kidney; Ligation; Liver Transplantation; Male; Middle Aged; Portal Vein; Regional Blood Flow; Renal Veins; Splenectomy; Splenorenal Shunt, Surgical; Vascular Surgical Procedures
PubMed: 28851020
DOI: 10.1111/ans.14044 -
Journal of Sports Sciences Jul 2017Mobile power meters provide a valid means of measuring cyclists' power output in the field. These field measurements can be performed with very good accuracy and... (Review)
Review
Mobile power meters provide a valid means of measuring cyclists' power output in the field. These field measurements can be performed with very good accuracy and reliability making the power meter a useful tool for monitoring and evaluating training and race demands. This review presents power meter data from a Grand Tour cyclist's training and racing and explores the inherent complications created by its stochastic nature. Simple summary methods cannot reflect a session's variable distribution of power output or indicate its likely metabolic stress. Binning power output data, into training zones for example, provides information on the detail but not the length of efforts within a session. An alternative approach is to track changes in cyclists' modelled training and racing performances. Both critical power and record power profiles have been used for monitoring training-induced changes in this manner. Due to the inadequacy of current methods, the review highlights the need for new methods to be established which quantify the effects of training loads and models their implications for performance.
Topics: Athletic Performance; Bicycling; Humans; Physical Conditioning, Human; Reproducibility of Results
PubMed: 27686573
DOI: 10.1080/02640414.2016.1215504 -
West African Journal of Medicine Oct 2023Caries is controlled by several factors including exposure to diets rich in carbohydrates, tooth susceptibility, and the presence of some oral flora bacteria such as...
BACKGROUND
Caries is controlled by several factors including exposure to diets rich in carbohydrates, tooth susceptibility, and the presence of some oral flora bacteria such as Streptococcus mutans (S. mutans) and Lactobacilli sp. Tooth demineralisation occurs when the pH in the oral cavity is lower than the critical pH of 5.5 which occurs because of the production of acid by the acidogenic bacteria in the oral flora when they break down sugars (substrate) for energy.
OBJECTIVE
The objective of this study was to determine the salivary Streptococcus mutans and Lactobacillus counts and their association with resting pH of saliva and dental caries in pre-school children in Lagos.
MATERIALSAND METHODS
Fifty preschoolers with and without caries were recruited from the dental clinic and staff primary school in Lagos University Teaching Hospital, Idi-araba respectively. Saliva was collected in Thioglycollate media. The resting pH of the saliva was measured immediately in the clinic with a pH meter (Jenway pH meter, PHS 25). In the laboratory, the samples were streaked on Mitis salivarius bacitracin agar (MSB) and Rogosa agar for S. mutans and Lactobacillus respectively and incubated anaerobically for 48 hours at 37°C. Biochemical tests and morphological characteristics of colonies were used to identify S. mutans and Lactobacillus.
RESULTS
The children were aged between 45 to 71 months with a mean age of 56.66 7.17 months. The mean age of the caries-free group was 55.48 7.57 months and that of caries active group was 57.84 6.76 months. The caries-active subjects recorded significantly higher bacterial counts of S. mutans and Lactobacillus compared to the cariesfree subjects. Streptococcus mutans and Lactobacillus showed a negative and highly significant correlation with resting pH (r = - 0.38 and r = - 0.32 value respectively).
CONCLUSION
The resting pH of saliva in both groups was within normal values. Streptococcus mutans and Lactobacillus counts were significantly inversely correlated to the resting pH value of saliva. Streptococcus mutans and Lactobacillus counts were significantly higher in the caries active group.
CONTEXTE
La carie est contrôlée par plusieurs facteurs dont l'exposition à des régimes riches en glucides, la sensibilité des dents et la présence de certaines bactéries de la flore buccale telles que Streptococcus mutans (S. mutans) et Lactobacilli sp.. La déminéralisation des dents se produit lorsque le pH dans la cavité buccale est inférieur au pH critique de 5,5 qui se produit à la suite de la production d'acide par les bactéries acidurogènes dans la flore buccale lorsqu'elles décomposent les sucres (substrat) pour l'énergie.
BUT ET OBJECTIF
L'objectif de cette étude était de déterminer le nombre de Streptococcus mutans et de Lactobacillus et leur association avec le pH au repos de la salive et des caries dentaires chez les enfants d'âge préscolaire à Lagos.
MATÉRIEL ET MÉTHODES
Cinquante enfants d'âge préscolaire avec et sans caries ont été recrutés respectivement à la clinique dentaire et à l'école primaire du personnel de l'hôpital universitaire de Lagos, Idiaraba. La salive a été recueillie dans un milieu au thioglycolate. Le pH au repos de la salive a été mesuré immédiatement en clinique avec un pHmètre (Jenway pH meter, PHS 25).Au laboratoire, les échantillons ont été striés sur gélose bacitracine Mitis salivarius (MSB) et gélose Rogosa pour S. mutans et Lactobacillus respectivement et incubés en anaérobiose pendant 48 heures à 37°C. Des tests biochimiques et des caractéristiques morphologiques des colonies ont été utilisés pour identifier S. mutans et Lactobacillus.
RÉSULTATS
Les enfants étaient âgés de 45 à 71 mois avec un âge moyen de 56,66 ± 7,17 mois. L'âge moyen du groupe sans carie était de 55,48 ± 7,57 mois et celui du groupe avec carie active était de 57,84 ± 6,76 mois. Les sujets actifs carieux ont enregistré un nombre de bactéries significativement plus élevé de S. mutans et Lactobacillus par rapport aux sujets sans caries. Streptococcus mutans et Lactobacillus ont montré une corrélation négative et hautement significative avec la valeur du pH au repos (r = - 0.38 et r = - 0.32 respectivement) .
CONCLUSION
Le pH au repos de la salive dans les deux groupes était dans les valeurs normales. Les numérations de Streptococcus mutans et de Lactobacillus étaient significativement inversement corrélées à la valeur du pH de la salive au repos. Les numérations de Streptococcus mutans et de Lactobacillus étaient significativement plus élevées dans le groupe actif carieux.
MOTS-CLÉS
Numération bactérienne, Streptococcus mutans, Lactobacillus, pH delasalive,Enfants d'âgepréscolaire.
Topics: Humans; Child, Preschool; Child; Streptococcus mutans; Saliva; Agar; Dental Caries; Nigeria; Cyclophosphamide; Doxorubicin
PubMed: 37906512
DOI: No ID Found