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Frontiers in Immunology 2020This study aimed to investigate the protective effects of 16 (Lac16) and a 10 (BSC10) against (Cp) infection in broilers. A total of 720 one-day-old chicks were...
This study aimed to investigate the protective effects of 16 (Lac16) and a 10 (BSC10) against (Cp) infection in broilers. A total of 720 one-day-old chicks were randomly divided into four groups. The control and Cp group were only fed a basal diet, while the two treatment groups received basal diets supplemented with Lac16 (1 × 10 cfu·kg) and BSC10 (1 × 10 cfu·kg) for 21 days, respectively. On day 1 and days 14 to 20, birds except those in the control group were challenged with 1 × 10 cfu type A strain once a day. The results showed that both Lac16 and BSC10 could ameliorate intestinal structure damage caused by infection. infection induced apoptosis by increasing the expression of Bax and and decreasing Bcl-2 expression and inflammation evidence by higher levels of , , and in the ileum mucosa, and NO production in jejunal mucosa, which was reversed by Lac16 and BSC10 treatment except for ( 0.05). Besides, the two probiotics restored the intestinal microbiota imbalance induced by infection, characterized by the reduced and and the increased at the phyla level and decreased and at the genus level. The two probiotics also reversed metabolic pathways of the microbiota in -infected broilers, including B-vitamin biosynthesis, peptidoglycan biosynthesis, and pyruvate fermentation to acetate and lactate II pathway. In conclusion, Lac16 and BSC10 can effectively protect broilers against infection through improved composition and metabolic pathways of the intestinal microbiota, intestinal structure, inflammation, and anti-apoptosis.
Topics: Animals; Chickens; Clostridium Infections; Clostridium perfringens; Lactobacillus plantarum; Paenibacillus polymyxa; Poultry Diseases
PubMed: 33679724
DOI: 10.3389/fimmu.2020.628374 -
Life Science Alliance Oct 2020is an agriculturally important plant growth-promoting rhizobacterium. Many species are known to be engaged in complex bacteria-bacteria and bacteria-host interactions,...
is an agriculturally important plant growth-promoting rhizobacterium. Many species are known to be engaged in complex bacteria-bacteria and bacteria-host interactions, which in other species were shown to necessitate quorum sensing communication. However, to date, no quorum sensing systems have been described in Here, we show that the type strain ATCC 842 encodes at least 16 peptide-based communication systems. Each of these systems is comprised of a pro-peptide that is secreted to the growth medium and processed to generate a mature short peptide. Each peptide has a cognate intracellular receptor of the RRNPP family, and we show that external addition of communication peptides leads to reprogramming of the transcriptional response. We found that these quorum sensing systems are conserved across hundreds of species belonging to the family, with some species encoding more than 25 different peptide-receptor pairs, representing a record number of quorum sensing systems encoded in a single genome.
Topics: Bacterial Proteins; Genome, Bacterial; Genomics; Paenibacillus; Paenibacillus polymyxa; Plant Development; Quorum Sensing
PubMed: 32764104
DOI: 10.26508/lsa.202000847 -
Plant Disease Mar 2024Strawberry is a popular fruit with valuable nutrition and an attractive fragrance, but its production and propagation are limited by various diseases, including...
Strawberry is a popular fruit with valuable nutrition and an attractive fragrance, but its production and propagation are limited by various diseases, including anthracnose and gray mold. For disease management, biological control measures are environmentally friendly and good alternatives to fungicides to avoid crop losses, reduce carbon emissions, and improve food safety. In this study, TP3, which originated from the strawberry phyllosphere, was shown to antagonize the anthracnose fungal pathogen and reduce leaf symptoms on strawberry plants. Several mass spectra corresponding to fusaricidin were detected in the confrontation assay of TP3 and by image mass spectrometry The transcription of and in the fusaricidin biosynthesis gene cluster increased while TP3 was cultured in the medium containing the culture filtrate of , as detected by reverse-transcription polymerase chain reaction, indicating the involvement of fusaricidins in TP3 antagonism against the anthracnose pathogen. Further disease control assays demonstrated the time frame and spatial mode of TP3-induced systemic resistance of strawberry against . The transcript level of the marker gene of the jasmonic acid pathway increased in strawberry leaves after drenching treatment with TP3, and the callose deposition was enhanced by further flg22 treatment. In addition, TP3 treatments of the strawberry mother plants reduced infection in the daughter plants, which would be a potent feature for the application of TP3 in strawberry nurseries and fields to reduce the impact of diseases, especially anthracnose.
Topics: Paenibacillus polymyxa; Fragaria; Fungicides, Industrial; Peptide Fragments; Thymopoietins
PubMed: 37580883
DOI: 10.1094/PDIS-08-23-1499-RE -
International Journal of Systematic and... Mar 2022Strain UY79 was isolated from a root nodule of , collected at the Esteros de Farrapos National Park, Río Negro, Uruguay. Cells were non-motile Gram-variable rods with...
Strain UY79 was isolated from a root nodule of , collected at the Esteros de Farrapos National Park, Río Negro, Uruguay. Cells were non-motile Gram-variable rods with central to subterminal oval to ellipsoidal endospores that swell the sporangia. Growth was observed in the range of 15-42 °C (optimum, 30 °C), pH 5.0-9.0 (optimum, pH 7.0-8.0) and with up to 3 % (w/v) NaCl (optimum, 1-2 %). Strain UY79 was facultative anaerobic, catalase-positive and oxidase-negative. According to the results of 16S rRNA gene sequence analysis, UY79 belongs to the genus and is closely related to MS2379, BD-57, ATCC 842 and PB172, exhibiting 99.4, 99.0, 99.0 and 98.9% sequence identity, respectively. Average nucleotide identity and digital DNA-DNA hybridization values with the most closely related type strains were 74.3-88.6% and 38.2-48.7 %, respectively. Major fatty acids (>10 %) were anteiso-C, iso-C and C. Menaquinones MK-7 and MK-6 were the only isoprenoid quinones detected. Major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and an unidentified glycolipid. Spermidine was the predominant polyamine. The DNA G+C content based on the draft genome sequence was 46.34 mol%. Based on the current polyphasic study, UY79 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is UY79 (=CCM 9147=CGMCC 1.19038).
Topics: Arachis; Bacterial Typing Techniques; Base Composition; DNA, Bacterial; Fatty Acids; Paenibacillus; Phylogeny; RNA, Ribosomal, 16S; Sequence Analysis, DNA
PubMed: 35324421
DOI: 10.1099/ijsem.0.005294 -
Molecules (Basel, Switzerland) Nov 2023Glycosidases are essential for the industrial production of functional oligosaccharides and many biotech applications. A novel β-galactosidase/α-L-arabinopyranosidase...
Glycosidases are essential for the industrial production of functional oligosaccharides and many biotech applications. A novel β-galactosidase/α-L-arabinopyranosidase (PpBGal42A) of the glycoside hydrolase family 42 (GH42) from KF-1 was identified and functionally characterized. Using NPG as a substrate, the recombinant PpBGal42A (77.16 kD) was shown to have an optimal temperature and pH of 30 °C and 6.0. Using NPαArap as a substrate, the optimal temperature and pH were 40 °C and 7.0. PpBGal42A has good temperature and pH stability. Furthermore, Na, K, Li, and Ca (5 mmol/L) enhanced the enzymatic activity, whereas Mn, Cu, Zn, and Hg significantly reduced the enzymatic activity. PpBGal42A hydrolyzed NP-β-D-galactoside and NP-α-L-arabinopyranoside. PpBGal42A liberated galactose from β-1,3/4/6-galactobiose and galactan. PpBGal42A hydrolyzed arabinopyranose at C20 of ginsenoside Rb2, but could not cleave arabinofuranose at C20 of ginsenoside Rc. Meanwhile, the molecular docking results revealed that PpBGal42A efficiently recognized and catalyzed lactose. PpBGal42A hydrolyzes lactose to galactose and glucose. PpBGal42A exhibits significant degradative activity towards citrus pectin when combined with pectinase. Our findings suggest that PpBGal42A is a novel bifunctional enzyme that is active as a β-galactosidase and α-L-arabinopyranosidase. This study expands on the diversity of bifunctional enzymes and provides a potentially effective tool for the food industry.
Topics: Paenibacillus polymyxa; Lactose; Molecular Docking Simulation; Galactose; Glycoside Hydrolases; Cloning, Molecular; beta-Galactosidase; Hydrogen-Ion Concentration; Substrate Specificity; Paenibacillus
PubMed: 38005185
DOI: 10.3390/molecules28227464 -
BioMed Research International 2020is a plant growth-promoting rhizobacterium that has immense potential to be used as an environmentally friendly replacement of chemical fertilizers and pesticides. In...
is a plant growth-promoting rhizobacterium that has immense potential to be used as an environmentally friendly replacement of chemical fertilizers and pesticides. In the present study, SK1 was isolated from bulbs of . The isolated endophytic strain showed antifungal activities against important plant pathogens like , , , and . The highest percentage of growth inhibition, i.e., 66.67 ± 2.23%, was observed for SK1 against followed by 61.19 ± 3.12%, 60.71 ± 3.53%, and 55.54 ± 2.89% against , , and , respectively. The metabolite profiling of ethyl acetate fraction was assessed through the UHPLC-LTQ-IT-MS/MS analysis, and putative identification was done with the aid of the GNPS molecular networking workflow. A total of 29 compounds were putatively identified which included dipeptides, tripeptides, cyclopeptides (cyclo-(Leu-Leu), cyclo(Pro-Phe)), 2-heptyl-3-hydroxy 4-quinolone, 6-oxocativic acid, anhydrobrazilic acid, 1-(5-methoxy-1H-indol-3-yl)-2-piperidin-1-ylethane-1,2-dione, octadecenoic acid, pyochelin, 15-hydroxy-5Z,8Z,11Z, 13E-eicosatetraenoic acid, (Z)-7-[(2R,3S)-3-[(2Z,5E)-Undeca-2,5-dienyl]oxiran-2-yl]hept-5-enoic acid, arginylasparagine, cholic acid, sphinganine, elaidic acid, gossypin, L-carnosine, tetrodotoxin, and ursodiol. The high antifungal activity of SK1 might be attributed to the presence of these bioactive compounds. The isolated strain SK1 showed plant growth-promoting traits such as the production of organic acids, ACC deaminase, indole-3-acetic acid (IAA), siderophores, nitrogen fixation, and phosphate solubilization. IAA production was strongly correlated with the application of exogenous tryptophan concentrations in the medium. Furthermore, inoculation of SK1 enhanced plant growth of two varieties, Tresor and White Heaven, under greenhouse condition. In the light of these findings, the SK1 may be utilized as a source of plant growth promotion and disease control in sustainable agriculture.
Topics: Anti-Infective Agents; Ascomycota; Carbon-Carbon Lyases; Carboxylic Acids; Endophytes; Fusarium; Indoleacetic Acids; Lilium; Nitrogen Fixation; Paenibacillus polymyxa; Plant Diseases; Plant Growth Regulators; Plant Roots; Siderophores; Tandem Mass Spectrometry
PubMed: 32190683
DOI: 10.1155/2020/8650957 -
Journal of Zhejiang University....In this study, plant growth-promoting rhizobacteria (PGPR) were evaluated as potential biocontrol agents against postharvest pathogens of apple fruits. In vitro...
In this study, plant growth-promoting rhizobacteria (PGPR) were evaluated as potential biocontrol agents against postharvest pathogens of apple fruits. In vitro bioassays revealed that, out of 30 isolates screened, isolates APEC136 and APEC170 had the most significant inhibitory effects against the mycelial growth of several fungal pathogens. Analysis of 16S ribosomal RNA (rRNA) sequences identified the two effective isolates as Paenibacillus polymyxa and Bacillus subtilis, respectively. The two strains showed greater growth in brain-heart infusion broth than in other growth media. Treatment of harvested apples with suspensions of either strain reduced the symptoms of anthracnose disease caused by two fungal pathogens, Colletotrichum gloeosporioides and Colletotrichum acutatum, and white rot disease caused by Botryosphaeria dothidea. Increased productions of amylase and protease by APEC136, and increased productions of chitinase, amylase, and protease by APEC170 might have been responsible for inhibiting mycelial growth. The isolates caused a greater reduction in the growth of white rot than of anthracnose. These results indicate that the isolates APEC136 and APEC170 are promising agents for the biocontrol of anthracnose and white rot diseases in apples after harvest, and suggest that these isolates may be useful in controlling these diseases under field conditions.
Topics: Antibiosis; Bacillus subtilis; Fruit; Malus; Paenibacillus polymyxa; Plant Diseases
PubMed: 27921398
DOI: 10.1631/jzus.B1600117 -
Frontiers in Microbiology 2019The Gram-positive rhizosphere bacterium promotes plant growth and produces various antibiotics. Herein, we review research on this species over the past two and a half... (Review)
Review
The Gram-positive rhizosphere bacterium promotes plant growth and produces various antibiotics. Herein, we review research on this species over the past two and a half decades, and focus on the mechanisms of strain E681, isolated from barley roots in the South Korea in 1995. Strain E681 has outstanding growth-promoting effects on barley, cucumber, pepper, sesame, and and produces antimicrobial compounds that protect plants against pathogenic fungi, oomycetes, and bacteria. Induced systemic resistance elicited by treating seeds or roots with strain E681 is a possible mechanism for protecting systemic plant tissues from biotic and other environmental stresses. Genome sequencing has broadened our horizons for antibiotic development and other industrial applications beyond agricultural use. At least six gene clusters for the biosynthesis of antibiotics have been discovered, including polymyxin (), which was recently re-instated as an antibiotic of last resort against Gram-negative drug-resistant bacteria. Three groups of antibiotic synthetases include the gene clusters that encode one for the non-ribosomal peptide polymyxin, fusaricidin, and tridecaptin, another for the lantibiotic paenilan, and the third for a polyketide. We successfully introduced the gene cluster into the surrogate host and created polymyxin derivatives by domain swapping. Furthermore, various E681 derivatives, including a high fusaricidin producer and strains lacking multi-antibiotics production, have been constructed by random mutagenesis and genome engineering. Thus, E681 is an important bacterium that contributes to both plant and human health.
PubMed: 30930873
DOI: 10.3389/fmicb.2019.00467 -
Scientific Reports Nov 2017Plant-parasitic nematodes (PPNs) cause serious crop losses worldwide. In this study, we investigated the nematicidal factors and the modes and mechanisms of action...
Plant-parasitic nematodes (PPNs) cause serious crop losses worldwide. In this study, we investigated the nematicidal factors and the modes and mechanisms of action involved in nematode control by Paenibacillus polymyxa KM2501-1. Treatment of the second-stage juveniles (J2) juveniles of PPN Meloidogyne incognita with the biological control agent KM2501-1 resulted in a mortality of 87.66% in vitro and reduced symptoms on tomato by up to 82.61% under greenhouse conditions. We isolated 11 volatile organic compounds (VOCs) from strain KM2501-1, of which 8 had contact nematicidal activity, 6 had fumigant activity, and 5 acted as stable chemotactic agents to M. incognita. The VOCs provided a comprehensive strategy against PPNs that included "honey-trap", fumigant, attractant and repellent modes. Furfural acetone and 2-decanol functioned as "honey-traps" attracting M. incognita and then killing it by contact or fumigation. Two other VOCs, 2-nonanone and 2-decanone, as well as strain KM2501-1 itself, destroyed the integrity of the intestine and pharynx. Collectively our results indicate that VOCs produced by P. polymyxa KM2501-1 act through diverse mechanisms to control M. incognita. Moreover, the novel "honey-trap" mode of VOC-nematode interaction revealed in this study extends our understanding of the strategies exploited by nematicidal biocontrol agents.
Topics: Alcohols; Aldehydes; Animals; Antinematodal Agents; Ketones; Paenibacillus polymyxa; Tylenchoidea
PubMed: 29176679
DOI: 10.1038/s41598-017-16631-8 -
Microorganisms Jun 2022Maize ( L.) is an important crop in the world and maize sheath blight damages the yield and quality greatly. In this study, an antagonist strain, which exhibited...
Maize ( L.) is an important crop in the world and maize sheath blight damages the yield and quality greatly. In this study, an antagonist strain, which exhibited antagonism against pathogenic fungi of maize and controlled maize banded leaf sheath blight in the field, was effectively isolated and named strain SF05. High cellulase and chitinase activity of the strain were detected in this study, which might contribute to degrading the cell wall of fungi. Furthermore, different resistant genes such as , and were elicited differently by the strain in the leaves and stems of maize. In order to explain the biocontrol mechanism of strain SF05, the genome was sequenced and then the genes involving the biocontrol mechanism including biofilm formation pathways genes, cell wall degradation enzymes, secondary metabolite biosynthesis gene clusters and volatile organic compounds biosynthesis genes were predicted. The study revealed the biocontrol mechanism of strain SF05 preliminary and laid a foundation for further research of biocontrol mechanism of .
PubMed: 35889037
DOI: 10.3390/microorganisms10071318