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FEMS Microbiology Reviews Nov 2015The bacterial genus Pantoea comprises many versatile species that have been isolated from a multitude of environments. Pantoea was delineated as a genus approximately 25... (Review)
Review
The bacterial genus Pantoea comprises many versatile species that have been isolated from a multitude of environments. Pantoea was delineated as a genus approximately 25 years ago, but since then, approximately 20 species have been identified having a diversity of characteristics. Isolates from water and soil have been harnessed for industrial purposes including bioremediation, and the degradation of herbicides and other toxic products. Other isolates possess nitrogen fixation and plant growth-promoting capabilities, which are currently being explored for agricultural applications. Some isolates are antibiotic producers, and have been developed into biocontrol agents for the management of plant diseases. Pantoea is also known to form host associations with a variety of hosts, including plants, insects and humans. Although often thought of as a plant pathogen, recent evidence suggests that Pantoea is being frequently isolated from the nosocomial environment, with considerable debate as to its role in human disease. This review will explore this highly versatile group and its capabilities, its known associations, and the underlying genetic and genomic determinants that drive its diversity and adaptability.
Topics: Animals; Biodiversity; Environmental Microbiology; Genome, Bacterial; Host-Pathogen Interactions; Humans; Industrial Microbiology; Pantoea; Phylogeny; Plants
PubMed: 26109597
DOI: 10.1093/femsre/fuv027 -
Journal of Equine Veterinary Science Sep 2020Pantoea agglomerans, family Enterobacteriaceae, is a Gram-negative bacterium that may be isolated from soil and from plants. This bacterium has been associated with...
Pantoea agglomerans, family Enterobacteriaceae, is a Gram-negative bacterium that may be isolated from soil and from plants. This bacterium has been associated with disease in plants, humans, and rarely in domestic animal species. We describe here a case of fibrinonecrotic placentitis and equine abortion associated with P. agglomerans infection in southern Brazil. A fetus with 10 months of gestation and its placenta were evaluated. Gross lesions were observed in the cervical star extending to the body of the chorioallantois and consisted of a focally extensive, transmural, severely thickened yellow area. Histologically, this area in the chorioallantois was effaced by severe necrosis, associated with marked inflammatory infiltrate of neutrophils and abundant deposition of fibrin and cellular debris. Aggregates of bacterial rods were noted intermixed with inflammation areas. No significant lesions were observed in the remaining organs inspected. Tissue samples of the lung, placenta, and stomach contents were cultured, and microbiological tests revealed the growth of P. agglomerans in all evaluated samples. The present study reaffirms the participation of P. agglomerans as a cause of bacterial placentitis and abortion in horses.
Topics: Animals; Brazil; Chorioamnionitis; Female; Horse Diseases; Horses; Pantoea; Placenta; Placenta Diseases; Pregnancy
PubMed: 32797784
DOI: 10.1016/j.jevs.2020.103156 -
Ecotoxicology (London, England) Aug 2023The application of synthetic pesticides is one of the fastest acting tools at farmers' disposal to prevent and mitigate the threats posed by plant pests in agriculture....
The application of synthetic pesticides is one of the fastest acting tools at farmers' disposal to prevent and mitigate the threats posed by plant pests in agriculture. However, the effects of these above-ground applications of pesticides are known to be detrimental to some belowground, non-target soil biota. At present, the effects many pesticides have on key functional microbial groups associated with phosphate (P) solubilization in the soil are still largely unknown. The purpose of this study was to compare the effects of two herbicides, glyphosate, and paraquat, on phosphate solubilizing bacteria (PSB) with and without pH adjustment (after herbicide addition) since pH is a major indicator of P solubilization. In our assay, two PSB strains (Pantoea agglomerans and Serratia rubidaea) were chosen to assess their ability to solubilize tricalcium phosphate (TCP) by using the vanadate-molybdate method (to measure the amount of P solubilized) in the presence of glyphosate (5.4 g/L and 10.8 g/L) or paraquat (2 g/L and 4 g/L) separately. To assess the effect of PSB treated by the herbicides, a growth experiment using PSB inoculated wheat seedlings was performed under greenhouse conditions (25 °C, light 16 h/8 h dark). After four weeks, wheat above-ground growth parameters were measured. Our results showed that even under recommended doses of glyphosate (5.4 g/L) and paraquat (2 g/L), a decrease in P solubilization activity was observed in P. agglomerans and S. rubidaea. Whilst paraquat affected TCP solubilization more than glyphosate with and without pH adjustment, there was a significant decrease (p < 0.05) in TCP solubilization, up to 39% and 93% in the presence of glyphosate and paraquat, respectively, for S. rubidaea, and up to 45% and 95% in the presence of glyphosate and paraquat, respectively, for P. agglomerans. The effect of the herbicides on the PSB had the same results as in the greenhouse test on wheat seedling growth, confirming that these herbicides have both above and belowground negative effects, despite being used at recommended doses.
Topics: Phosphates; Herbicides; Soil; Pantoea; Paraquat
PubMed: 37407783
DOI: 10.1007/s10646-023-02681-4 -
International Journal of Molecular... Jan 2023The bacterial genus comprises species found in a variety of different environmental sources. spp. are often recovered from plant material and are capable of both...
The bacterial genus comprises species found in a variety of different environmental sources. spp. are often recovered from plant material and are capable of both benefitting the plants and acting like phytopathogens. Some species of (including ) are considered opportunistic human pathogens capable of causing various infections in immunocompromised subjects. In this study, a strain of (identified by 16S rRNA gene sequencing) was isolated from a dead specimen of an unidentified Latvian grasshopper species. The retrieved strain of was then used as a host for the potential retrieval of phages from the same source material. After rounds of plaque purification and propagation, three high-titer lysates corresponding to putatively distinct phages were acquired. Transmission electron microscopy revealed that one of the phages was a myophage with an unusual morphology, while the two others were typical podophages. Whole-genome sequencing (WGS) was performed for each of these isolated phages. Genome de novo assembly and subsequent functional annotation confirmed that three different strictly lytic phages were isolated. Elaborate genomic characterization of the acquired phages was performed to elucidate their place within the so-far-uncovered phage diversity.
Topics: Humans; Bacteriophages; Pantoea; RNA, Ribosomal, 16S
PubMed: 36768143
DOI: 10.3390/ijms24031820 -
Journal of Applied Microbiology Jul 2021Isolation, characterization and identification of possible microbial contaminant(s) in the inflated foil bag containing hop pellets packed and stored in a modified...
AIMS
Isolation, characterization and identification of possible microbial contaminant(s) in the inflated foil bag containing hop pellets packed and stored in a modified atmosphere.
METHODS AND RESULTS
Package gas of the inflated foil bag containing hop pellets was analysed by gas chromatography. Compared with the reference modified atmosphere, containing about 16 vol.% of CO , the inflated bag atmosphere contained 53 vol.% CO , suggesting possible microbial contamination. Therefore, several standard and mineral media, with added hop pellets or hop infusion, were used for cultivation at different temperatures under an anaerobic atmosphere. Cultivation in mineral medium with hop pellets yielded a bacterial isolate that was identified by MALDI-TOF mass spectrometry and verified by partial 16S rRNA gene analysis as Pantoea agglomerans, a known plant epiphyte.
CONCLUSIONS
A novel strain of P. agglomerans (designed as DBM 3696) was found to be suspicious of causing inflation of the foil bag containing dried hop pellets packed in modified atmosphere.
SIGNIFICANCE AND IMPACT OF THE STUDY
This study suggests that P. agglomerans, probably hop epiphyte, could cause sporadic inflation of bags with hop pellets packed in modified atmosphere causing logistical problems during bags transport.
Topics: Atmosphere; Food Packaging; Food Storage; Humulus; Mass Spectrometry; Pantoea; RNA, Ribosomal, 16S
PubMed: 33320407
DOI: 10.1111/jam.14970 -
Plant Disease Oct 2022Species of represent a group of plant pathogenic bacteria that infect a variety of agro-economically important plant species. Among these, a complex of , , , and...
Species of represent a group of plant pathogenic bacteria that infect a variety of agro-economically important plant species. Among these, a complex of , , , and subsp. cause center rot in onion, resulting in significant economic losses. As species of are phenotypically closely related, identification of species relies on the sequencing and phylogenetic analysis of housekeeping genes. To aid in rapid identification of species, efforts have been made in developing species-specific primers to be used in PCR assays. In the current study, two , one , one , and three published primers as well as newly developed PagR primers were evaluated for their specificity against 79 strains, belonging to 15 different species. To ensure that selected primers were evaluated against accurately identified species, sequencing and phylogenetic analysis of housekeeping gene were conducted. Thereafter, PCR assays using selected species-specific primers were performed. The results showed that previously described -specific PANA_1008; -specific allii-leuS; -specific PANST_rpoB, 3614galE, and DC283galE primers; and one newly designed -specific PagR primer pair were highly specific for their target species. They accurately identified these strains into their species and, in some cases, their subspecies level. The findings of the current study will facilitate rapid and reliable identification of , , , and
Topics: Pantoea; Phylogeny; Polymerase Chain Reaction; Species Specificity
PubMed: 35171633
DOI: 10.1094/PDIS-08-21-1810-SC -
Molecular Plant-microbe Interactions :... Feb 2023The phytopathogen belongs to the Bacteria, Proteobacteria, Gammaproteobacteria, Enterobacterales, Erwiniaceae in species classification. It causes disease symptoms in...
The phytopathogen belongs to the Bacteria, Proteobacteria, Gammaproteobacteria, Enterobacterales, Erwiniaceae in species classification. It causes disease symptoms in many plants such as corn, banana, and walnut. This study aimed to report the complete genome of CHTF15, which represents the first whole-genome sequence of an isolate from diseased walnut leaves. The total length of the assembled genome was 4,820,607 bp, with an average GC content of 55.3%, including a circular chromosome and three circular plasmids, two of which were previously unreported sequences and one was announced previously. The CHTF15 genome helps understand the pathogenic mechanism of this important plant pathogen and provides an important theoretical basis for disease epidemic and field control. [Formula: see text] The author(s) have dedicated the work to the public domain under the Creative Commons CC0 "No Rights Reserved" license by waiving all of his or her rights to the work worldwide under copyright law, including all related and neighboring rights, to the extent allowed by law, 2023.
Topics: Pantoea; Juglans; Plasmids
PubMed: 36693088
DOI: 10.1094/MPMI-10-22-0216-A -
Microorganisms Sep 2020Bacteriophages are viruses capable of recognizing with high specificity, propagating inside of, and destroying their bacterial hosts. The phage lytic life cycle makes...
Bacteriophages are viruses capable of recognizing with high specificity, propagating inside of, and destroying their bacterial hosts. The phage lytic life cycle makes phages attractive as tools to selectively kill pathogenic bacteria with minimal impact on the surrounding microbiome. To effectively harness the potential of phages in therapy, it is critical to understand the phage-host dynamics and how these interactions can change in complex populations. Our model examined the interactions between the plant pathogen , the antagonistic epiphyte , and the bacteriophages that infect and kill both species. strains are used as a phage carrier; their role is to deliver and propagate the bacteriophages on the plant surface prior to the arrival of the pathogen. Using liquid cultures, the populations of the pathogen, carrier, and phages were tracked over time with quantitative real-time PCR. The jumbo phage ϕEa35-70 synergized with both the ϕEa21-4 and ϕEa46-1-A1 and was most effective in combination at reducing growth over 24 h. Phage ϕEa35-70, however, also reduced the growth of . Phage cocktails of ϕEa21-4, ϕEa46-1-A1, and ϕEa35-70 at multiplicities of infections (MOIs) of 10, 1, and 0.01, respectively, no longer inhibited growth of . When this cocktail was grown with for 8 h prior to pathogen introduction, pathogen growth was reduced by over four log units over 24 h. These findings present a novel approach to study complex phage-host dynamics that can be exploited to create more effective phage-based therapies.
PubMed: 32971807
DOI: 10.3390/microorganisms8091449 -
Archives of Microbiology Aug 2019The genus Pantoea contains a broad range of plant-associated bacteria, including some economically important plant pathogens as well as some beneficial members effective... (Review)
Review
The genus Pantoea contains a broad range of plant-associated bacteria, including some economically important plant pathogens as well as some beneficial members effective as biological control agents of plant pathogens. The most well-characterized representatives of biological control agents from this genus generally produce one or more antimicrobial compounds adding to biocontrol efficacy. Some Pantoea species evaluated as biocontrol agents for fire blight disease of apple and pear produce a histidine-reversible antibiotic. Three commonly studied histidine-reversible antibiotics produced by Pantoea spp. are herbicolin O, MccEh252, and pantocin A. Pantocin A is a novel ribosomally encoded and post-translationally modified peptide natural product. Here, we review the current knowledge on the chemistry, genetics, biosynthesis, and incidence and environmental relevance of pantocin A and related histidine-reversible antibiotics produced by Pantoea.
Topics: Biological Control Agents; Glycopeptides; Pantoea; Peptides; Plant Diseases
PubMed: 30868174
DOI: 10.1007/s00203-019-01647-7 -
Ecotoxicology and Environmental Safety Oct 2018Interactions between microorganisms and minerals have the potential contribution to remove polycyclic aromatic hydrocarbons (PAHs) in model systems. In this study,...
Interactions between microorganisms and minerals have the potential contribution to remove polycyclic aromatic hydrocarbons (PAHs) in model systems. In this study, phenanthrene (PHE) was used as a probe molecule to explore the potential adsorption and biotransformation processes in the presence of microorganisms and various reference clays, such as montmorillonite (M), kaolinite (K), and pyrophyllite (P). Equilibrium adsorption experiments and scanning electron microscopy (SEM) technique were used to investigate the sorption of Pantoea agglomerans strains on clay minerals saturated with cations (Na and Fe). The adsorption isotherms of PHE and Pantoea agglomerans strains on cation-modified clay minerals fitted to Langmuir equation, and their adsorbed amounts both followed the sequence: montmorillonite > kaolinite > pyrophyllite. For six types of cation-modified minerals, the behavior of PHE adsorbed and Pantoea agglomerans adhered onto mentioned minerals was in the order of Na(I)-M > Fe(Ⅲ)-M, Na(I)-K > Fe(Ⅲ)-K and Fe(Ⅲ)-P > Na(I)-P, respectively. The biodegradation results showed that cation-modified clay minerals could enhance the biodegradation of PHE, ascribing to their large specific surface area, and cation exchange capability, as well as the difference in zeta potential between minerals and Pantoea agglomerans strains. Comparison of biodegradation rates displayed that PHE was degraded the highest in the presence of Na-M (93.285%). In addition, the obtained results suggested that the adhesion of bacteria onto cation-exchanged clay minerals was beneficial to the biodegradation of PHE. Anthracen-9-ylmethanol and 3,4-dimethyl-2-(3-methylbutanoyl)benzoic acid were detected as the main intermediate compounds, which can be further biodegraded into small molecules. The overall results obtained in this study are of valuable significance for the understanding of the behavior of PHE in soil and associated environment.
Topics: Adsorption; Aluminum Silicates; Bentonite; Biodegradation, Environmental; Cations; Clay; Ferric Compounds; Kaolin; Minerals; Pantoea; Phenanthrenes; Sodium; Soil Pollutants
PubMed: 29886310
DOI: 10.1016/j.ecoenv.2018.05.091