-
Enzyme and Microbial Technology Jan 2020A mediator biosensor based on Paracoccus yeei bacteria for assaying the toxicity of perfumery and cosmetics samples was developed. An approach to selecting an...
A mediator biosensor based on Paracoccus yeei bacteria for assaying the toxicity of perfumery and cosmetics samples was developed. An approach to selecting an electron-transport mediator based on the heterogeneous electron transfer constants for investigated mediators (k) and the mediator-biomaterial interaction constants (k) was proposed. Screening of nine compounds as potential mediators showed a ferrocene mediator immobilized in graphite paste to have the highest efficiency of electron transfer to the graphite-paste electrode (the heterogeneous transfer constant, 0.4 ± 0.1 cm/s) and a high constant of interaction with P. yeei (0.023 ± 0.001 dm/(g·s)). A biosensor for toxicity assessment based on the ferrocene mediator and P. yeei bacteria was formed. The biosensor was tested on samples of four heavy metals (Cu, Zn, Pb, Cd) and two phenols (phenol and p-nitrophenol). Proceeding from the EC index, it was found that the use of the ferrocene mediator made the biosensor more sensitive to investigated toxicants than most analogues described. Toxicity determination of four perfumery and cosmetics samples by the developed biosensor showed prospects of using this system for real-time toxicity monitoring of samples.
Topics: Biosensing Techniques; Metals, Heavy; Nitrophenols; Paracoccus; Perfume; Phenols; Toxicity Tests
PubMed: 31731957
DOI: 10.1016/j.enzmictec.2019.109435 -
Chemosphere Sep 2023Microbial consortia HY3 and JY3 with high degradation efficiency of 2-Diethylamino-4-hydroxy-6-methylpyrimidine (DHMP) were isolated from aerobic and parthenogenic ponds...
Microbial consortia HY3 and JY3 with high degradation efficiency of 2-Diethylamino-4-hydroxy-6-methylpyrimidine (DHMP) were isolated from aerobic and parthenogenic ponds of DHMP-containing pharmaceutical wastewater, respectively. Both consortia were enriched and reached stable degradation performance with a DHMP concentration of 1500 mg L. The DHMP degradation efficiencies of HY3 and JY3 were 95.66% ± 0.24% and 92.16% ± 2.34% under the condition of shaking at 180 r·min and the temperature of 30 °C for 72 h. And the removal efficiencies of chemical oxygen demand were 89.14% ± 4.78% and 80.30% ± 11.74%, respectively. High-throughput sequencing results indicated that three bacterial phyla of Proteobacteria, Bacteroidetes, and Actinobacteria were dominant in both HY3 and JY3, but their dominances varied. At the genus level, the richness of Unclassified Comamonadaceae (34.23%), Paracoccus (14.75%), and Brevundimonas (13.94%) ranked top three in HY3 whereas Unclassified Comamonadaceae (40.80%), Unclassified Burkholderiales (13.81%) and Delftia (13.11%) were dominant in JY3. The metabolites of DHMP degradation by HY3 and JY3 were analyzed in detail. Two pathways for cleavage of the nitrogenous heterocyclic ring were speculated, one of which was identified for the first time in this study.
Topics: Wastewater; Microbial Consortia; Bacteria; Heterocyclic Compounds; Pharmaceutical Preparations; Bioreactors
PubMed: 37217008
DOI: 10.1016/j.chemosphere.2023.139000 -
Bioresource Technology 2015Two bacterial strains capable of degrading N,N-dimethylformamide (DMF) were isolated from the effluent and sludge samples of textile and tyre industries. The 16S rRNA...
Two bacterial strains capable of degrading N,N-dimethylformamide (DMF) were isolated from the effluent and sludge samples of textile and tyre industries. The 16S rRNA gene analysis revealed that bacterial strains belonged to the genera Paracoccus and named as Paracoccus sp. MKU1 and Paracoccus sp. MKU2. The DMF degradation experiments conducted at a DMF concentration of 1% v/v and HPLC analysis revealed that MKU1 and MKU2 degraded 55% and 46% of DMF after 120 h of growth. Biofilm quantification by microtiter plate assay revealed that both the bacterial isolates can form efficient biofilm on during DMF utilization. The presence of secondary carbon sources influenced the DMF degradation and biofilm formation where highest biofilm formation was observed in the presence of acetate and enhanced the DMF degradation to a maximum of 86.59% with MKU1 whereas glucose and acetate enhanced DMF degradation by MKU2 to a maximum of 82.7% and 80% respectively.
Topics: Acetates; Biodegradation, Environmental; Biofilms; Carbon; Chromatography, High Pressure Liquid; Dimethylformamide; Glucose; Kinetics; Nitrogen; Paracoccus; Phylogeny; RNA, Ribosomal, 16S; Sewage; Species Specificity
PubMed: 25728343
DOI: 10.1016/j.biortech.2015.02.042 -
International Journal of Systematic and... Apr 2023Currently, the genus comprises 76 recognized species. Members of are mostly isolated from environmental, animal, and plant sources. This report describes and proposes...
Currently, the genus comprises 76 recognized species. Members of are mostly isolated from environmental, animal, and plant sources. This report describes and proposes a novel species of isolated from clinical specimens of the human ocular surface. We isolated two aerobic, Gram-stain-negative, non-spore-forming, coccoid or short rod-shaped, and non-motile strains (designated DK398 and DK608) from conjunctival sac swabs of two healthy volunteers. The results showed that the strains grew best under the conditions of 28°C, pH 7.0, and 1.0 % (w/v) NaCl. Sequence analysis based on the 16S rRNA gene showed that strains DK398 and DK608 were members of , most similar to 43P (98.54 and 98.62 %), GHD-05 (98.34 and 98.41 %), and NB88 (98.21 and 98.29 %). Phenotypic analysis showed that DK398 and DK608 were positive for catalase and oxidase, negative for producing N-acetyl-β-glucosaminic acid, arginine dihydrolase, and -glucuronidase but positive for leucine arylamidase. The predominant isoprenoid quinone was Q-10, and the major polar lipids included phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, and an unidentified glycolipid. The major fatty acids (>10%) were summed feature 8 (C ω7c and/or C ω6c) and C. The meso-diaminopimelic acid was found in the cell wall peptidoglycan of DK398. The major cell wall sugars were ribose and galactose. Based on the results of phylogenetic analyses, low (<83.22%) average nucleotide identity, digital DNA-DNA hybridization (<26.0%), chemotaxonomic analysis, and physiological properties, strain DK398 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is DK398 (=CGMCC 1.17227=JCM 33719).
Topics: Animals; Humans; Fatty Acids; Phospholipids; Phylogeny; RNA, Ribosomal, 16S; Sequence Analysis, DNA; Ubiquinone; Bacterial Typing Techniques; DNA, Bacterial; Base Composition; Paracoccus
PubMed: 37068015
DOI: 10.1099/ijsem.0.005838 -
Environmental Research Nov 2023The impact of environmental pollution in air and water is reflected mainly in the soil ecosystem as it impairs soil functions. Also, since the soil is the habitat for...
The impact of environmental pollution in air and water is reflected mainly in the soil ecosystem as it impairs soil functions. Also, since the soil is the habitat for billions of organisms, the biodiversity is in turn altered. Microbes are precise sensors of ecological contamination, and bacteria have a key and important function in terms of bioremediation of the contaminated soil. Hence in the current work, we aimed at assessing the unidentified bacterial population through Illumina MiSeq sequencing technology and their community structural changes in different levels of petroleum-contaminated soil and sludge samples (aged, sludge, and leakage soil) to identify unique bacteria for their potential application in remediation. The studies showed that major bacterial consortiums namely, Proteobacteria (57%), Alphaproteobacteria (31%), and Moraxellaceae (23%) were present in aged soil, whereas Proteobacteria (52%), Alphaproteobacteria (33%), and Rhodobacteraceae (28%) were dominantly found in sludge soil. In leakage soil, Proteobacteria (59%), Alphaproteobacteria (33%), and Rhodobacteraceae (29%) were abundantly present. The Venn diagrams are used to analyze the distribution of abundances in individual operational taxonomic units (OTUs) within three soil samples. After data filtering, they were grouped into OTU clusters and 329 OTUs were identified from the three soil samples. Among the 329, 160 OTUs were common in the three soil samples. The bacterial diversity is estimated using alpha diversity indices and Shanon index and was found to be 4.490, 4.073 and 4.631 in aged soil, sludge soil and leakage soil, respectively and similarly richness was found to be 618, 417 and 418. The heat map was generated by QIIME software and from the top 50 enriched genera few microbes such as Pseudomonas, Bacillus, Mycobacterium, Sphingomonas and Paracoccus, were shown across all the samples. In addition, we also analyzed various physicochemical properties of soil including pH, temperature, salinity, electrical conductivity, alkalinity, total carbon, total organic matter, nitrogen, phosphorus and potassium to calculate the soil quality index (SQI). The SQI of aged, sludge and leakage soil samples were 0.73, 0.64, and 0.89, respectively. These findings show the presence of unexplored bacterial species which could be applied for hydrocarbon remediation and further they can be exploited for the same.
Topics: Petroleum; Ecosystem; Sewage; Soil Pollutants; Soil Microbiology; RNA, Ribosomal, 16S; Bacteria; Biodegradation, Environmental; Soil; Genetic Variation
PubMed: 37517495
DOI: 10.1016/j.envres.2023.116779 -
Bioresource Technology Dec 2021A novel symbiotic system combined by heterotrophic nitrification-aerobic denitrification (HN-AD) mixed bacteria and Chlorella pyrenoidosa was firstly proposed to resolve...
Effect of bacteria-to-algae volume ratio on treatment performance and microbial community of a novel heterotrophic nitrification-aerobic denitrification bacteria-chlorella symbiotic system.
A novel symbiotic system combined by heterotrophic nitrification-aerobic denitrification (HN-AD) mixed bacteria and Chlorella pyrenoidosa was firstly proposed to resolve the poor tolerance and nitrogen removal performance of traditional symbiotic system for treating high ammonia biogas slurry. Results showed that the volume ratio of bacteria to algae had significant effects on nitrogen removal efficiency, microbial community structure, functional bacteria and genes. The optimal ratio was 1/3, and the average removal efficiency of TN and TP increased by 28.9% and 67.6% respectively, compared to those of HN-AD bacteria. High-throughput sequencing indicated nitrogen removal was jointly completed by HN-AD and heterotrophic denitrification. HN-AD bacteria Halomonas and Pseudomonas played a key role in nitrogen removal, and Rhodocyclaceae and Paracoccus took an important part in phosphorus removal. According to the functional gene prediction, the total relative abundance of nitrogen removal genes (0.0127%) and narG, narH and narL genes (0.0054%) were highest in 1/3 system.
Topics: Aerobiosis; Bacteria; Chlorella; Denitrification; Heterotrophic Processes; Microbiota; Nitrification; Nitrogen
PubMed: 34600093
DOI: 10.1016/j.biortech.2021.126025 -
International Journal of Systematic and... Dec 2018A Gram-stain-negative, short ovoid- to coccus-shaped, aerobic, non-flagellated, and nonmotile strain, designated WN007, was isolated from the natural saline-alkali...
A Gram-stain-negative, short ovoid- to coccus-shaped, aerobic, non-flagellated, and nonmotile strain, designated WN007, was isolated from the natural saline-alkali wetland soil. Growth occurred at 10-45 °C (optimum 33-37 °C), pH 6.5-10.0 (optimum, pH 7.5-8.0) and with 0-15 % (w/v) NaCl (optimum, 2-4 % NaCl). Catalase- and oxidase-positive. A comparison of the 16S rRNA gene sequence of WN007 showed the highest sequence similarities to Paracoccus chinensis (97.5 %) and Paracoccus niistensis (97.4 %). The major respiratory quinone of strain WN007 was Q10 and the fatty acid profile of strain WN007 contained a predominant amount of summed feature 7 and small quantities of C10 : 0 3OH, C16 : 00 and C18 : 00. The major polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylcholine, glycolipid, aminolipid and lipid. The genome revealed that the G+C content was 63.9 mol% and the DNA-DNA relatedness values between strain WN007 and the type strains of P. chinensis CGMCC 1.7655and P. niistensis KCTC 22789 were 46.9±2.3 and 42.4±1.7 %, respectively. This was also confirmed by the low average nucleotide identity values (<83.5 %) between strain WN007 and the most closely related recognized Paracoccus species. According to these results, strain WN007 represents a novel species of the genus Paracoccus, for which the name Paracoccussalipaludis sp. nov. is proposed. The type strain is WN007 (=KCTC 52851=ACCC 19972).
Topics: Bacterial Typing Techniques; Base Composition; China; DNA, Bacterial; Fatty Acids; Nucleic Acid Hybridization; Paracoccus; Phospholipids; Phylogeny; RNA, Ribosomal, 16S; Salinity; Sequence Analysis, DNA; Soil Microbiology; Ubiquinone
PubMed: 30325298
DOI: 10.1099/ijsem.0.003065 -
Current Microbiology Apr 2024A novel Paracoccus-related strain, designated YLB-12, was isolated from a sediment sample from the tidal zone of Shapowei Port, Xiamen, Fujian Province, PR China. The...
A novel Paracoccus-related strain, designated YLB-12, was isolated from a sediment sample from the tidal zone of Shapowei Port, Xiamen, Fujian Province, PR China. The novel strain is a Gram-stain-negative, short, rod-shaped, nonmotile, catalase- and oxidase-positive strain that grows at 10-37 °C and pH 5.0-9.0 in the presence of 0-12.0% (w/v) NaCl. Phylogenetic analysis of the 16S rRNA gene sequences indicated that this strain belongs to the genus Paracoccus and that its highest sequence similarity was to Paracoccus homiensis DD-R11 (98.5%), followed by Paracoccus zeaxanthinifaciens ATCC 21588 (97.4%), Paracoccus rhizosphaerae LMG 26205 (97.2%), Paracoccus beibuensis CGMCC 1.7295 (97.1%) and Paracoccus halotolerans CFH 90064 (97.0%). The DNA‒DNA hybridization values between strain YLB-12 and the five closely related type strains ranged from 20.4 to 22.4%. The genomic G+C content of strain YLB-12 was 63.7%. In addition to diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine and phosphatidylglycerol, the polar lipids of the strain YLB-12 also consisted of an unidentified glycolipid and four unidentified polar lipids. The cells contained summed feature 8 (Cω6c /Cω7c, 62.7%) as the major cellular fatty acid and ubiquinone-10 as the predominant menaquinone. On the basis of its phenotypic and genotypic characteristics, strain YLB-12 represents a novel species within the genus Paracoccus, for which the name Paracoccus maritimus sp. nov. is proposed. The type strain was YLB-12 (= MCCC 1A17213 = KCTC 82197).
Topics: Phylogeny; RNA, Ribosomal, 16S; Fatty Acids; Paracoccus; DNA
PubMed: 38592513
DOI: 10.1007/s00284-024-03637-5 -
International Journal of Systematic and... Aug 2023Strain designated TK19116 was isolated from the shallow-sea hydrothermal systems off Kueishantao Island in Taiwan, China. The bacterium was Gram-stain-negative, aerobic,...
Strain designated TK19116 was isolated from the shallow-sea hydrothermal systems off Kueishantao Island in Taiwan, China. The bacterium was Gram-stain-negative, aerobic, oxidase-positive and catalase-positive. Cells of the strain TK19116 were short-rod-shaped and non-motile. The results of phylogenetic analysis of 16S rRNA gene sequences indicated that strain TK19116 belonged to the genus , with the highest sequence similarity to 4-2 (97.1 %). The average nucleotide identity values between the strain TK19116 with 4-2, J6, M26 and BM15 were 75.3, 76.7, 76.7 and 75.8%, respectively. The digital DNA-DNA hybridization value between the strain TK19116 with 4-2, J6, M26 and BM15 were 19.7, 20.3, 20.5 and 20.0%, respectively. The main respiratory quinone of strain TK19116 was ubiquinone 10. The polar lipids include aminolipid, phosphatidylcholine, diphosphatidylglycerol, glycolipid, phosphatidylglycerol and phospholipid. The principal fatty acid of strain TK19116 was summed feature 8 (C 6 and/or C 7). The G+C content of the chromosomal DNA was 64.2 %. The combination of the results of the phylogenetic, phenotypic and chemotaxonomic analysis, strain TK19116 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is TK19116 (= MCCC 1K08025=JCM 35527).
Topics: Fatty Acids; Phylogeny; RNA, Ribosomal, 16S; DNA, Bacterial; Base Composition; Bacterial Typing Techniques; Sequence Analysis, DNA; Phospholipids; Paracoccus
PubMed: 37535057
DOI: 10.1099/ijsem.0.005998 -
Biotechnology and Bioengineering Mar 2024The biodegradation of chloroethene compounds under oxic and anoxic conditions is well established. However, the biological reactions that take place under microoxic...
The biodegradation of chloroethene compounds under oxic and anoxic conditions is well established. However, the biological reactions that take place under microoxic conditions are unknown. Here, we report the biostimulated (BIOST: addition of lactate) and natural attenuated (NAT) degradation of chloroethene compounds under microoxic conditions by bacterial communities from chloroethene compounds-contaminated groundwater. The degradation of tetrachloroethene was significantly higher in NAT (15.14% on average) than in BIOST (10.13% on average) conditions at the end of the experiment (90 days). Sporomusa, Paracoccus, Sedimentibacter, Pseudomonas, and Desulfosporosinus were overrepresented in NAT and BIOST compared to the source groundwater. The NAT metagenome contains phenol hydrolase P1 oxygenase (dmpL), catechol-1,2-dioxygenase (catA), catechol-2,3-dioxygenases (dmpB, todE, and xylE) genes, which could be involved in the cometabolic degradation of chloroethene compounds; and chlorate reductase (clrA), that could be associated with partial reductive dechlorination of chloroethene compounds. Our data provide a better understanding of the bacterial communities, genes, and pathways potentially implicated in the reductive and cometabolic degradation of chloroethene compounds under microoxic conditions.
Topics: Bacteria; Tetrachloroethylene; Lactic Acid; Biodegradation, Environmental; Catechols
PubMed: 38116701
DOI: 10.1002/bit.28630