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Preparative Biochemistry & Biotechnology Jul 2024In this study, we tried to explore the influence of various tricarboxylic acid (TCA) cycle intermediates on carotenoid production and with a focus on enhancing pigment...
In this study, we tried to explore the influence of various tricarboxylic acid (TCA) cycle intermediates on carotenoid production and with a focus on enhancing pigment biosynthesis, we conducted two statistical analysis. In case of TCA intermediates influence on pigment production by RSPO1; fumaric acid, and malic acid were observed as potent enhancers of pigment biosynthesis. Further, to optimize key media components for enhanced carotenoid production, the Plackett-Burman design was employed encompassing carbon, nitrogen sources, TCA cycle intermediates, and metal salts. The selected factors after Plackett Burman were fine-tuned through Response Surface Methodology and the optimal concentrations that have remarkably elevated carotenoid production were starch-2.24 g/l, MgSO-0.416 g/l, ZnSO-0.0157 g/l, and fumaric Acid-16 mM. Further, evaluation of pigment cytotoxicity against normal (Vero) and Non-Small Cell Carcinoma (A549) cells was performed. The resultant IC values were quantified as 161.3 µg/ml and 7.623 µg/ml for Vero and A549 cells, respectively. Moreover, a reactive oxygen species (ROS) determination study in A549 cells was done which have shown a noteworthy threefold ROS production in A549 cells through fluorescence spectroscopic observation. This implies that the bacterial carotenoids can act as potent pro-oxidants against cancerous cells while being nontoxic toward normal cells.
Topics: Chlorocebus aethiops; Animals; Humans; A549 Cells; Vero Cells; Carotenoids; Paracoccus; Culture Media; Reactive Oxygen Species
PubMed: 38165781
DOI: 10.1080/10826068.2023.2282533 -
Chemosphere Feb 2021Tetracycline (TC) in soil severely imperils food security and ecosystem function. Metaphire guillelmi is a common species in farmland. It could impact the degradation of...
Tetracycline (TC) in soil severely imperils food security and ecosystem function. Metaphire guillelmi is a common species in farmland. It could impact the degradation of antibiotics. However, how it affects is rarely unknown. Hence, the present study aimed to investigate the effects of M. guillelmi on the TC degradation in soil and the changes of the antioxidant system and intestinal bacteria in M. guillelmi. The treatments that M. guillelmi was inoculated on soil contaminated with different TC concentrations were contrasted with those without M. guillelmi. After 21 days, the degradation rate of TC significantly increased by 13.70%, 18.14% and 29.01% at 10, 50 and 100 mg kg TC dose, respectively, due to the inoculation of M. guillelmi. The half-life of TC was also shortened nearly by 1/3 to 2/3. Superoxide dismutase (SOD) increased in a dose-dependent manner with the increase of TC concentration on the 7th and 14th day. Catalase (CAT) and glutathione S-transferase (GST) presented an inverted U-shaped dose response on the 7th day, and the peak of enzyme activities occurred at TC concentration of 0.1, 1 mg kg (CAT) and 0.1 mg kg (GST). Malondialdehyde (MDA) contents did not change significantly. At the phylum level, only Verrucomicrobia significantly decreased under 1 mg kg and 100 mg kg TC dose. Genus Paracoccus, Singulisphaera, Acinetobacter and Bacillus significantly increased and became the dominant bacterium during the TC degradation. Overall, the antioxidant system and intestinal bacteria of M. guillelmi were affected by the different concentrations of TC pollution, which provided new ideas for the research of mechanism of TC degradation by earthworms in the future.
Topics: Animals; Anti-Bacterial Agents; Antioxidants; Bacteria; Catalase; Ecosystem; Oligochaeta; Soil; Soil Pollutants; Superoxide Dismutase
PubMed: 33279238
DOI: 10.1016/j.chemosphere.2020.129097 -
Scientific Reports May 2022The aim of this study was to evaluate an effect of short and medium chain carboxylic acids (CAs) rich stream derived from acidogenic mixed culture fermentation of acid...
The aim of this study was to evaluate an effect of short and medium chain carboxylic acids (CAs) rich stream derived from acidogenic mixed culture fermentation of acid whey on polyhydroxyalkanoates (PHAs) synthesis by Paracoccus homiensis and compare it with the impact of individual synthetic CAs. The obtained results confirmed that the analyzed bacterium is able to metabolize synthetic CAs as the only carbon sources in the growth medium with maximum PHAs production yields of 26% of cell dry mass (CDM). The replacement of the individual CAs by a CAs-rich residual stream was found to be beneficial for the Paracoccus homiensis growth. The highest biomass concentration reached about 2.5 g/L with PHAs content of 17% of CDM. The purified PHAs were identified as poly(3-hydroxybutyrate-co-3-hydroxyvalerate) by applying gas chromatography coupled with mass spectrometry, Fourier transform infrared spectroscopic spectra and UV-Vis spectra. Furthermore, a differential scanning calorimetric, thermogravimetric and water contact angle analysis proved that the extracted copolymers have useful properties. The obtained data are promising in the perspective of developing a microbial PHAs production as a part of an integrated valorization process of high CAs content waste-derived streams.
Topics: Acids; Carboxylic Acids; Culture Media; Gas Chromatography-Mass Spectrometry; Paracoccus; Polyhydroxyalkanoates
PubMed: 35508573
DOI: 10.1038/s41598-022-11114-x -
Environmental Research Jan 2022University students are constantly exposed to potential bacterial pathogens and environmental pollutants in indoor environment because they spend most of their time...
University students are constantly exposed to potential bacterial pathogens and environmental pollutants in indoor environment because they spend most of their time indoors. University dormitory and printing shop are two typical indoor environments frequented by university students. However, little is known about the characteristics of bacterial community as well as the effect of indoor environmental factors on them. 16S rRNA gene sequencing was used to reveal the bacterial community in indoor dust, electronic devices were recorded during dust sampling, and polybrominated diphenyl ethers (PBDEs) were detected by gas chromatography mass spectrometry (GC-MS). Proteobacteria, Actinobacteria and Firmicutes were leading phyla, and Acinetobacter, Paracoccus and Kocuria were dominating genera. The predominant genera showed Acinetobacter > Paracoccus > unidentified Corynebacteriaceae in indoor dusts from university dormitories, whereas Paracoccus > unidentified Cyanobacteria > Acinetobacter in printing shops. The occurrence of Acinetobacter, Kocuria, Corynebacterium, Pseudomonas, and Bacillus suggested the health risks of potential pathogenic bacteria to university students. Significant differences of microbial composition and diversity were proved between university dormitories and printing shops. Chemoheterotrophy and aerobic chemoheterotrophy were dominant bacterial functions, and the seven primary bacterial functions displayed university dormitory > printing shop. BDE 138 and BDE 66 were main environmental parameters affecting the indoor dust bacterial community in university dormitory, while printer and BDE 47 played dominating role in shaping microorganism in printing shop. The complex biotic (potential bacterial pathogens) and abiotic factors (electronic equipment and chemical pollutants) in indoor dusts may pose potential health risks to university students.
Topics: Air Pollution, Indoor; Bacteria; Dust; Environmental Monitoring; Halogenated Diphenyl Ethers; Humans; RNA, Ribosomal, 16S; Students; Universities
PubMed: 34411549
DOI: 10.1016/j.envres.2021.111869 -
Scientific Reports May 2019Bacteria of the genus Paracoccus inhabit various pristine and anthropologically-shaped environments. Many Paracoccus spp. have biotechnological value and several are...
Bacteria of the genus Paracoccus inhabit various pristine and anthropologically-shaped environments. Many Paracoccus spp. have biotechnological value and several are opportunistic human pathogens. Despite extensive knowledge of their metabolic potential and genome architecture, little is known about viruses of Paracoccus spp. So far, only three active phages infecting these bacteria have been identified. In this study, 16 Paracoccus strains were screened for the presence of active temperate phages, which resulted in the identification of five novel viruses. Mitomycin C-induced prophages were isolated, visualized and their genomes sequenced and thoroughly analyzed, including functional validation of their toxin-antitoxin systems. This led to the identification of the first active Myoviridae phage in Paracoccus spp. and four novel Siphoviridae phages. In addition, another 53 prophages were distinguished in silico within genomic sequences of Paracoccus spp. available in public databases. Thus, the Paracoccus virome was defined as being composed of 66 (pro)phages. Comparative analyses revealed the diversity and mosaicism of the (pro)phage genomes. Moreover, similarity networking analysis highlighted the uniqueness of Paracoccus (pro)phages among known bacterial viruses.
Topics: Computer Simulation; DNA, Viral; Genome, Viral; Genomics; Microscopy, Electron, Transmission; Mitomycin; Molecular Sequence Annotation; Mosaicism; Myoviridae; Paracoccus; Prophages; Siphoviridae
PubMed: 31133656
DOI: 10.1038/s41598-019-44460-4 -
Archives of Microbiology Aug 2021A gram-stain-negative, non-motile and rod-shaped strain, designated wg1, was isolated from activated sludge obtained from wastewater treatment plant in Binzhou (Shandong...
A gram-stain-negative, non-motile and rod-shaped strain, designated wg1, was isolated from activated sludge obtained from wastewater treatment plant in Binzhou (Shandong province, PR China). Growth of strain wg1 occurred at 25-45 °C (optimum, 37 °C), at pH 7.0-9.0 (optimum growth at pH 8.0) and at a salinity range of 0-4% (optimum, 1%). The chemotaxonomic, phenotypic and genomic traits were investigated. The 16S rRNA gene sequence analysis showed that strain wg1 belonged to the genus Paracoccus. The species with highest similarity to strain wg1 was Paracoccus communis VKM B-2787 (98.27%), followed by Paracoccus kondratievae VKM B-2222 (98.25%). The isoprenoid quinone was Q-10. Major cellular fatty acids were summed feature 8, C and C. The major polar lipids were diphosphatidylglycerol (DPG), phosphatidylethanolamine (PE), aminoglycolipid (AGL), phosphatidylglycerol (PG), phosphatidylcholine (PC), aminolipid (AL), one unidentified lipid (L) and one unidentified phospholipid (PL). The genome size was 4,834,448 bp with a G+C content of 67.67 mol%. The prediction result of secondary metabolites based on genome has shown that the strain wg1 contained 12 clusters, and the gene involved in primary metabolism showed differences in the comparison between wg1 and reference strains. The dDDH values of strain wg1 with P. communis VKM B-2787, P. kondratievae VKM B-2222 and P. denitrificans DSM 413 were 45.30, 30.60 and 39.50%, respectively. Based on its physiological properties, chemotaxonomic characteristics and low ANI and dDDH results, strain wg1 is considered to represent a novel species for which the name Paracoccus binzhouensis sp. nov., is proposed. The type strain is wg1 (= KCTC 72861 = CCTCC AB 2019400).
Topics: Bacterial Typing Techniques; China; DNA, Bacterial; Fatty Acids; Paracoccus; Phospholipids; Phylogeny; RNA, Ribosomal, 16S; Sequence Analysis, DNA; Sewage; Species Specificity
PubMed: 33774710
DOI: 10.1007/s00203-021-02286-7 -
Environmental Science and Pollution... Apr 2018All locust epiphytic bacteria were screened and a total of 62 epiphytic bacteria were obtained from samples of Acrida cinerea. Via phylogenetic analysis, the 62...
All locust epiphytic bacteria were screened and a total of 62 epiphytic bacteria were obtained from samples of Acrida cinerea. Via phylogenetic analysis, the 62 epiphytic bacteria were allocated to 27 genera, 18 families, 13 orders, six classes, and four phylums. Then, cyhalothrin degradation experiments were conducted, and the 10 strains that degraded more than 30% cyhalothrin and Paracoccus acridae SCU-M53 showed the highest cyhalothrin degradation rate of 70.5%. Furthermore, Paracoccus acridae SCU-M53 was selected for optimal cyhalothrin biodegradation conditions via the response surface method (Design-Expert). Under the optimum conditions (28 °C, 75 mg/L, and 180 rpm), the cyhalothrin degradation rate reached 79.84% after 2 days. This suggests the possibility that isolating biodegradation cyhalothrin strains from Acrida cinerea is feasible.
Topics: Animals; Biodegradation, Environmental; Biodiversity; China; Environmental Monitoring; Environmental Pollutants; Grasshoppers; Nitriles; Paracoccus; Pesticides; Phylogeny; Pyrethrins
PubMed: 29427271
DOI: 10.1007/s11356-018-1410-y -
International Journal of Systematic and... Apr 2020A Gram-stain-negative, non-motile and ovoid bacterial strain, designated 4-2, was isolated from oil-contaminated water which was collected from Xinjiang Province,...
A Gram-stain-negative, non-motile and ovoid bacterial strain, designated 4-2, was isolated from oil-contaminated water which was collected from Xinjiang Province, north-west PR China. The 16S rRNA gene sequence analysis showed that strain 4-2 belonged to the genus . The species with highest similarity to strain 4-2 was YIM 90738 (97.83 %), followed by '' M26 (97.83 %) and SYSUP0003 (97.25 %). The average nucleotide identity values between 4-2 and three type strains were 84.69, 77.88 and 74.07 %, respectively. The genomic DNA G+C content of strain 4-2 was 61.4 mol%. Chemotaxonomical characteristic results showed that the respiratory quinone was ubiquinone Q-10 and the major fatty acids were summed feature 8 (C 7 or C 6) and C cyclo 8. The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, unidentified phospholipids, an unidentified aminolipid and an unidentified polar lipid. The predominant polyamines were putrescine, cadaverine and spermidine. On the basis of phenotypic, chemotaxonomic and phylogenetic inferences, strain 4-2 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is 4-2 (=CGMCC 1.13669=LMG 30882).
Topics: Bacterial Typing Techniques; Base Composition; China; DNA, Bacterial; Fatty Acids; Nucleic Acid Hybridization; Oil and Gas Fields; Paracoccus; Phospholipids; Phylogeny; RNA, Ribosomal, 16S; Sequence Analysis, DNA; Ubiquinone; Water Microbiology
PubMed: 32039746
DOI: 10.1099/ijsem.0.004036 -
Bioresource Technology Jun 2024Acetaminophen (APAP) is a frequently used, over-the-counter analgesic and antipyretic medication. Considering increase in global consumption, its ubiquity in environment...
Acetaminophen (APAP) is a frequently used, over-the-counter analgesic and antipyretic medication. Considering increase in global consumption, its ubiquity in environment with potential toxic impacts has become a cause of great concern. Hence, bioremediation of this emerging contaminant is of paramount significance. The present study incorporates a microcosm centric omics approach to gain in-depth insights into APAP degradation by Paracoccus sp. APAP_BH8. It can metabolize APAP (300 mg kg) within 16 days in soil microcosms. Genome analysis revealed potential genes capable of mediating degradation includes M20 aminoacylase family protein, guanidine deaminase, 4-hydroxybenzoate 3-monooxygenase, and 4-hydroxyphenylpyruvate dioxygenase. Whole proteome analysis showed differential expression of enzymes and bioinformatics provided evidence for stable binding of intermediates at the active site of considered enzymes. Metabolites identified were 4-aminophenol, hydroquinone, and 3-hydroxy-cis, cis-muconate. Therefore, Paracoccus sp. APAP_BH8 with versatile enzymatic and genetic attributes can be a promising candidate for formulating improved in situ APAP bioremediation strategies.
Topics: Acetaminophen; Biodegradation, Environmental; Proteomics; Genomics; Paracoccus; Metabolomics; Proteome
PubMed: 38677386
DOI: 10.1016/j.biortech.2024.130732 -
Bioresource Technology Oct 2019Eight species of mixotrophic sulfide oxidizing bacteria (SOB) were isolated from activated sludge and identified using 16S rRNA sequence analysis. The effects of organic...
Eight species of mixotrophic sulfide oxidizing bacteria (SOB) were isolated from activated sludge and identified using 16S rRNA sequence analysis. The effects of organic substances, dissolved oxygen (DO) and nitrate on sulfide oxidation and bacterial growth were studied in this work. The results showed that Paracoccus sp. (N1), Pseudomonas sp. (N2) and Pseudomonas sp. (S4) have strong adaptability to environments with low DO and high concentrations of organic substance. An SOB additive was optimized in artificial, odorous water. The optimized SOB additive is ablendof 80% N1 and 20% N2 bacteria solution with absorbance equal to 0.5 at a wavelength of 600 nm (OD), and the optimal dose of the additive is 20 ml/L. Oxidation-reduction potential (ORP), ammonia-nitrogen (NH-N) and released HS in an odorous river were measured with and without SOB additive, and the results indicated that the optimized SOB additive has excellent performance for odorous river bioremediation.
Topics: Bacteria; Biodegradation, Environmental; Oxidation-Reduction; RNA, Ribosomal, 16S; Sulfides; Sulfur; Water
PubMed: 31301572
DOI: 10.1016/j.biortech.2019.121721