-
Yonsei Medical Journal Jul 2017In Korea, registration of paraquat-containing herbicides was canceled in November 2011, and sales thereof were completely banned in November 2012. We evaluated the...
PURPOSE
In Korea, registration of paraquat-containing herbicides was canceled in November 2011, and sales thereof were completely banned in November 2012. We evaluated the effect of the paraquat ban on the epidemiology and mortality of herbicide-induced poisoning.
MATERIALS AND METHODS
This retrospective study analyzed patients treated for herbicide poisoning at 17 emergency departments in South Korea between January 2010 and December 2014. The overall and paraquat mortality rates were compared pre- and post-ban. Factors associated with herbicide mortality were evaluated using logistic analysis. To determine if there were any changes in the mortality rates before and after the paraquat sales ban and the time point of any such significant changes in mortality, R software, version 3.0.3 (package, bcp) was used to perform a Bayesian change point analysis.
RESULTS
We enrolled 2257 patients treated for herbicide poisoning (paraquat=46.8%). The overall and paraquat poisoning mortality rates were 40.6% and 73.0%, respectively. The decreased paraquat poisoning mortality rate (before, 75% vs. after, 67%, p=0.014) might be associated with increased intentionality. The multivariable logistic analysis revealed the paraquat ban as an independent predictor that decreased herbicide poisoning mortality (p=0.035). There were two major change points in herbicide mortality rates, approximately 3 months after the initial paraquat ban and 1 year after complete sales ban.
CONCLUSION
This study suggests that the paraquat ban decreased intentional herbicide ingestion and contributed to lowering herbicide poisoning-associated mortality. The change point analysis suggests a certain timeframe was required for the manifestation of regulatory measures outcomes.
Topics: Bayes Theorem; Demography; Female; Geography; Herbicides; Humans; Logistic Models; Male; Middle Aged; Multivariate Analysis; Paraquat; Poisoning; Republic of Korea
PubMed: 28541002
DOI: 10.3349/ymj.2017.58.4.859 -
Science Progress 2021The aquatic ecosystem is under increasing pressure from environmental contaminants due to anthropogenic activities. This study investigated the potential of glyphosate...
The aquatic ecosystem is under increasing pressure from environmental contaminants due to anthropogenic activities. This study investigated the potential of glyphosate and paraquat to induce DNA damage and other cell abnormalities in juvenile African Catfish, . Juvenile fish were exposed for 96 h to 0.36, 0.48, 0.60, 0.72 and 0.84 mg/L glyphosate; and 0.018, 0.037, 0.055, 0.110 and 0.221 mg/L paraquat. Following the exposure, the fish liver and blood were analysed for DNA damage and micronucleus respectively. DNA damage was analysed using comet assay while the micronucleus test was used for assessing nuclear abnormalities. Both herbicides induced DNA damage in fish, with paraquat exhibiting higher toxicity. The severity in liver DNA damage was observed to be dependent on concentration. The herbicides triggered formation of micronuclei, bean-shaped cells, lobed nuclei, and apoptosis in blood cells of fish. Both herbicides also increased the frequency of occurrence of these cell abnormalities in erythrocytes and showed mutagenic potential in fish. Glyphosate and Paraquat both have mutagenic potentials in fish and this is a reflection of the threat these contaminants pose to fish and other forms of aquatic life in our natural water bodies. Low concentrations of these herbicides should be encouraged when usage is inevitable.
Topics: Animals; Catfishes; DNA Damage; Ecosystem; Glycine; Herbicides; Mutagens; Paraquat; Water Pollutants, Chemical; Glyphosate
PubMed: 34148463
DOI: 10.1177/00368504211021751 -
International Journal of Environmental... Jun 2018This study aimed to assess paraquat concentrations in the urine of women at 28 weeks of pregnancy, delivery and 2 months postpartum and in the meconium of neonates. In...
This study aimed to assess paraquat concentrations in the urine of women at 28 weeks of pregnancy, delivery and 2 months postpartum and in the meconium of neonates. In all, 79 pregnant women were recruited from three hospitals located in agricultural areas in Thailand. The subjects were interviewed about personal characteristics, agricultural activities and pesticide use patterns. Paraquat was analyzed in urine and meconium using high performance liquid chromatography equipped with a fluorescence detector. The geometric mean (GSD) of urinary paraquat concentrations at 28 weeks of pregnancy, delivery and 2 months postpartum were 2.04 (4.22), 2.06 (5.04) and 2.42 (5.33) ng/mL, respectively. The urinary paraquat concentrations at 28 weeks of pregnancy, delivery and 2 months postpartum between agriculturist and non-agriculturist were not significantly different ( = 0.632, = 0.915, = 0.57 respectively). The geometric mean (GSD) of paraquat concentration in the meconium was 33.31 (4.59) ng/g. The factors predicting paraquat exposures among pregnant women and neonates included working outside, living near farmland, having family members who work on a farm, drinking well water and using herbicides or paraquat.
Topics: Adult; Agriculture; Environmental Exposure; Female; Humans; Infant, Newborn; Maternal Exposure; Meconium; Paraquat; Pesticides; Postpartum Period; Pregnancy; Pregnant Women; Thailand; Young Adult
PubMed: 29865285
DOI: 10.3390/ijerph15061163 -
QJM : Monthly Journal of the... May 2022
Topics: Herbicides; Humans; Paraquat
PubMed: 35294038
DOI: 10.1093/qjmed/hcac077 -
Microglia-derived exosomal circZNRF1 alleviates paraquat-induced neuronal cell damage via miR-17-5p.Ecotoxicology and Environmental Safety Sep 2023Paraquat (PQ) is an environmental poison that causes clinical symptoms similar to those of Parkinson's disease (PD) in vitro and in rodents. It can lead to the...
Paraquat (PQ) is an environmental poison that causes clinical symptoms similar to those of Parkinson's disease (PD) in vitro and in rodents. It can lead to the activation of microglia and apoptosis of dopaminergic neurons. However, the exact role and mechanism of microglial activation in PQ-induced neuronal degeneration remain unknown. Here, we isolated the microglia-derived exosomes exposed with 0 and 40 μM PQ, which were subsequently co-incubated with PQ-exposed neuronal cells to simulate intercellular communication. First, we found that exosomes released from microglia caused a change in neuronal cell vitality and reversed PQ-induced neuronal apoptosis. RNA sequencing data showed that these activated microglia-derived exosomes carried large amounts of circZNRF1. Moreover, a bioinformatics method was used to study the underlying mechanism of circZNRF1 in regulating PD, and miR-17-5p was predicted to be its target. Second, an increased Bcl2/Bax ratio could play an anti-apoptotic role. Bcl2 was predicted to be a downstream target of miR-17-5p. Our results showed that circZNRF1 plays an anti-apoptotic role by absorbing miR-17-5p and regulating the binding of Bcl2 after exosomes are internalized by dopaminergic neurons. In conclusion, we demonstrated a new intercellular communication mechanism between microglia and neurons, in which circZNRF1 plays a key role in protecting against PQ-induced neuronal apoptosis through miR-17-5p to regulate the biological process of PD. These findings may offer a novel approach to preventing and treating PD.
Topics: Microglia; Paraquat; Dopaminergic Neurons; Proto-Oncogene Proteins c-bcl-2; MicroRNAs
PubMed: 37591128
DOI: 10.1016/j.ecoenv.2023.115356 -
Plant Communications May 2022Paraquat (PQ) is the third most used broad-spectrum nonselective herbicide around the globe after glyphosate and glufosinate. Repeated usage and overreliance on this... (Review)
Review
Paraquat (PQ) is the third most used broad-spectrum nonselective herbicide around the globe after glyphosate and glufosinate. Repeated usage and overreliance on this herbicide have resulted in the emergence of PQ-resistant weeds that are a potential hazard to agriculture. It is generally believed that PQ resistance in weeds is due to increased sequestration of the herbicide and its decreased translocation to the target site, as well as an enhanced ability to scavenge reactive oxygen species. However, little is known about the genetic bases and molecular mechanisms of PQ resistance in weeds, and hence no PQ-resistant crops have been developed to date. Forward genetics of the model plant Arabidopsis thaliana has advanced our understanding of the molecular mechanisms of PQ resistance. This review focuses on PQ resistance loci and resistance mechanisms revealed in Arabidopsis and examines the possibility of developing PQ-resistant crops using the elucidated mechanisms.
Topics: Arabidopsis; Crops, Agricultural; Herbicide Resistance; Herbicides; Paraquat; Plant Weeds
PubMed: 35576161
DOI: 10.1016/j.xplc.2022.100321 -
Environmental Toxicology and... Mar 2023Given the paucity of research surrounding the effect of chronic paraquat on striatal neurotoxicity, there is a need for further investigation into the neurotoxic effects...
Given the paucity of research surrounding the effect of chronic paraquat on striatal neurotoxicity, there is a need for further investigation into the neurotoxic effects of paraquat in mouse striatum. Furthermore, while previous studies have shown that inhibiting soluble epoxide hydrolase mitigates MPTP-mediated endoplasmic reticulum stress in mouse striatum, its effect on paraquat toxicity is still unknown. Thus, this study attempts to observe changes in inflammatory and endoplasmic reticulum stress markers in mouse striatum following chronic paraquat administration to determine whether inhibiting soluble epoxide hydrolase mitigates paraquat-induced neurotoxicity and whether it can reduce TLR4-mediated inflammation in primary astrocytes and microglia. Our results show that while the pro-inflammatory effect of chronic paraquat is small, there is a significant induction of inflammatory and cellular stress markers, such as COX2 and CHOP, that can be mitigated through a prophylactic administration of a soluble epoxide hydrolase inhibitor.
Topics: Mice; Animals; Paraquat; Epoxide Hydrolases; Rodentia; Neurotoxicity Syndromes; Inflammation
PubMed: 36682504
DOI: 10.1016/j.etap.2023.104070 -
Langmuir : the ACS Journal of Surfaces... Jan 2023Although paraquat is a widely used herbicide, it is toxic to humans if ingested or absorbed through an open wound. To improve the safety of paraquat, a new formulation...
Although paraquat is a widely used herbicide, it is toxic to humans if ingested or absorbed through an open wound. To improve the safety of paraquat, a new formulation of paraquat based on photoresponsive polymers was exploited. Photoresponsive β-cyclodextrin polymer microspheres (AZO-CD) were synthesized via a host-guest interaction between β-cyclodextrin and azobenzene. AZO-CD were characterized by Fourier transform infrared spectrometry, circular dichroism, ultraviolet (UV) spectrophotometry, and thermogravimetric analysis, and their photoresponsiveness was also evaluated. AZO-CD were used to load paraquat, which yielded photoresponsive paraquat-loaded microspheres. For the paraquat-loaded microspheres, irradiation with UV light or sunlight induced the isomerization of azobenzene into the cis form. Then, the cis-azobenzene was liberated from the cavities of the β-cyclodextrin. The paraquat-loaded microspheres released paraquat continuously over time. Furthermore, under UV light, the herbicidal capacity of the paraquat-loaded microspheres against barnyard grass was comparable to that of free paraquat at the same dose. Our findings show that loading paraquat into AZO-CD provides a safe and environmentally friendly herbicide formulation.
Topics: Humans; Paraquat; Microspheres; beta-Cyclodextrins; Azo Compounds; Polymers; Herbicides
PubMed: 36542801
DOI: 10.1021/acs.langmuir.2c03004 -
International Journal of Environmental... Mar 2021Extensive use of herbicides is common among rural agricultural workers in Sri Lanka. Recent studies have postulated their role in the development of chronic kidney...
Extensive use of herbicides is common among rural agricultural workers in Sri Lanka. Recent studies have postulated their role in the development of chronic kidney disease of unknown etiology (CKDu). Paraquat and glyphosate are leading herbicides used by sugarcane farmers (SF), hence occupational exposure is inevitable. This study examined the expression of urinary paraquat, glyphosate and biomarkers among residential SF in CKDu emerging regions, Warunagama (WA) and Rahathangama (RH), in the Uva Province with non-endemic Matara (MA) in the Southern Province of Sri Lanka. Urinary glyphosate, Paraquat, kidney injury molecule -1 (KIM-1), neutrophil gelatinase-associated lipocalin (NGAL) and β2-microglobulin (B2M) were determined using enzyme-linked immunosorbent assays (ELISA). Urinary creatinine, microalbumin, serum creatinine (SCr), serum cystatin C, estimated glomerular filtration rate (eGFR), and albumin creatinine ratio (ACR) were also assessed. Generally, herbicide residues and kidney injury biomarkers were higher in SF compared to the non-endemic MA. Creatinine-adjusted urinary glyphosate and paraquat levels were significantly higher in WA compared to MA. ACR in RH (median 14.9; IQR 5.4-393.1 mg/g) and WA (23.7; 11.5-64.6) was significantly higher than MA (4.3; 2.2-6.7). This study reports 39 individuals with impaired kidney function among SF in Sri Lanka for the first time. Urinary NGAL levels were significantly higher in both WA (median 2.14; IQR 1.28-6.15 ng/mg Cr) and RH (3.09; 1.15-9.09) compared to MA (1.28; 0.56-2.81). However, urinary KIM-1 levels in RH (3.2; 1.29-106.1 ng/g Cr) and WA (3.6; 1.94-115.1) were not significantly higher in MA (1.74; 0.76-116.9). Urinary NGAL (r = 0.493), eGFR (r = -0.147) and ACR (r = 0.171) significantly correlated with urinary glyphosate, but not with urinary paraquat levels. Urinary KIM-1 levels did not correlate with either urinary glyphosate or paraquat, while urinary B2M and serum cystatin C levels showed significant correlation with urinary glyphosate levels. The current study reports higher urinary herbicide levels among sugarcane farmers in WA and RH, and that is potentially linked to the subsequent decline in kidney function, as indicated by ACR, eGFR, and NGAL. We posit that these indicators may serve as markers to detect renal injury among herbicide-exposed SF in Rural Sri Lanka.
Topics: Agriculture; Biomarkers; Creatinine; Glycine; Humans; Paraquat; Sri Lanka; Glyphosate
PubMed: 33810013
DOI: 10.3390/ijerph18063278 -
Nature Communications Jul 2018Paraquat, as one of the most widely used herbicides globally, is highly toxic to humans, and chronic exposure and acute ingestion leads to high morbidity and mortality...
Paraquat, as one of the most widely used herbicides globally, is highly toxic to humans, and chronic exposure and acute ingestion leads to high morbidity and mortality rates. Here, we report user-friendly, photo-responsive paraquat-loaded supramolecular vesicles, prepared via one-pot self-assembly of amphiphilic, ternary host-guest complexes between cucurbit[8]uril, paraquat, and an azobenzene derivative. In this vesicle formulation, paraquat is only released upon UV or sunlight irradiation that converts the azobenzene derivative from its trans- to its cis- form, which in turn dissociates the ternary host-guest complexations and the vesicles. The cytotoxicity evaluation of this vesicle formulation of paraquat on in vitro cell models, in vivo zebrafish models, and mouse models demonstrates an enhanced safety profile. Additionally, the PQ-loaded vesicles' herbicidal activity against a model of invasive weed is nearly identical to that of free paraquat under natural sunlight. This study provides a safe yet effective herbicide formulation.
Topics: Animals; Azo Compounds; COS Cells; Chlorocebus aethiops; Female; Herbicides; Humans; Kidney; Liver; Mice; Mice, Inbred C57BL; Models, Animal; Paraquat; Zebrafish
PubMed: 30054483
DOI: 10.1038/s41467-018-05437-5