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Molecular Cell Oct 2022The dietary factor vitamin K has been found to protect against ferroptosis, a form of cell death driven by lipid peroxidation. This reveals new dietary links to cancers...
The dietary factor vitamin K has been found to protect against ferroptosis, a form of cell death driven by lipid peroxidation. This reveals new dietary links to cancers and degenerative conditions and a key factor involved in warfarin poisoning.
Topics: Ferroptosis; Vitamin K; Warfarin; Lipid Peroxidation; Cell Death
PubMed: 36270246
DOI: 10.1016/j.molcel.2022.10.001 -
Sheng Wu Gong Cheng Xue Bao = Chinese... Nov 2022Fermentation is a green, low-carbon and sustainable process for the production of food, chemicals, fuels, and materials by using microbial strains as biocatalysts and... (Review)
Review
Fermentation is a green, low-carbon and sustainable process for the production of food, chemicals, fuels, and materials by using microbial strains as biocatalysts and renewable resources such as starch and biomass as feedstocks. China has the world's largest fermentation industry, the scale of amino acids, vitamins, and some other fermentation products accounted for 60%-80% of the global market share. The development of fermentation industry is of great significance for the strategic goal of "carbon neutralization and carbon peak" and the development of bioeconomy. Microbial strains are the core of fermentation industry, which directly decide what kind of chemical can be produced from what kind of feedstock at what cost. Innovating industrial strains to improve the conversion efficiency of raw materials, increase the production level, and expand product portfolio is the key to the high-quality development of fermentation industry. In recent years, the development of synthetic biology and systems biology has further deepened the understanding of the physiological and metabolic mechanisms of microbial chassis and accelerated the development of gene editing and other enabling technologies for strain design and engineering. All these advances have provided new driving force for the upgrading of industrial strains. This review focused on the representative fermentation products including amino acids, B vitamins, citric acid, and bio-ethanol. The latest progress of strain development for fermentation industry was reviewed from the perspective of basic research and technology innovation for industrial microbial chassis. How the integration of artificial intelligence and automation with life science will reshape the upgrading of industrial strains was also discussed.
Topics: Fermentation; Artificial Intelligence; Vitamin K; Amino Acids; Carbon
PubMed: 37699686
DOI: 10.13345/j.cjb.220611 -
European Journal of Cancer Prevention :... Mar 2020This review summarizes the current evidence on the potential role of phytol, a microbial metabolite of chlorophyl A, and its metabolites, phytanic and pristanic acids,... (Review)
Review
This review summarizes the current evidence on the potential role of phytol, a microbial metabolite of chlorophyl A, and its metabolites, phytanic and pristanic acids, in carcinogenesis. Primary food sources in Western diets are the nut skin for phytol and lipids in dairy, beef and fish for its metabolites. Phytol and its metabolites gained interest as dietary compounds for cancer prevention because, as natural ligands of peroxisome proliferator-activated receptor-α and -γ and retinoid X receptor, phytol and its metabolites have provided some evidence in cell culture studies and limited evidence in animal models of anti-carcinogenic, anti-inflammatory and anti-metabolic-syndrome properties at physiological concentrations. However, there may be a narrow range of efficacy, because phytol and its metabolites at supra-physiological concentrations can cause in vitro cytotoxicity in non-cancer cells and can cause morbidity and mortality in animal models. In human studies, evidence for a role of phytol and its metabolites in cancer prevention is currently limited and inconclusive. In short, phytol and its metabolites are potential dietary compounds for cancer prevention, assuming the challenges in preventing cytotoxicity in non-cancer cells and animal models and understanding phytol metabolism can be mitigated.
Topics: Animals; Butter; Carcinogenesis; Diet Surveys; Diet, Western; Dietary Supplements; Disease Models, Animal; Fatty Acids; Feeding Behavior; Humans; Neoplasms; Nuts; PPAR alpha; PPAR gamma; Phytanic Acid; Phytol; Retinoid X Receptors; Risk Assessment
PubMed: 31436750
DOI: 10.1097/CEJ.0000000000000534 -
MMW Fortschritte Der Medizin Nov 2022
Topics: Humans; Anticoagulants; Fibrinolytic Agents; Vitamin K; Kidney
PubMed: 36310260
DOI: 10.1007/s15006-022-2045-y -
MMW Fortschritte Der Medizin Jul 2022
Topics: Humans; Neoplasms; Vitamin K; Vitamins
PubMed: 35817897
DOI: 10.1007/s15006-022-1280-6 -
Revue Medicale Suisse Aug 2022
Topics: Diabetes Complications; Diabetes Mellitus; Humans; Vitamin K
PubMed: 36004661
DOI: 10.53738/REVMED.2022.18.792.1571 -
Clinical Chemistry Sep 2017
Topics: Blood Coagulation Tests; Prothrombin Time; Vitamin K
PubMed: 28848027
DOI: 10.1373/clinchem.2016.269498 -
Clinical Chemistry Sep 2017
Topics: Blood Coagulation Tests; Prothrombin Time; Vitamin K
PubMed: 28848028
DOI: 10.1373/clinchem.2017.272054 -
Magyar Sebeszet Dec 2023Egy 44 éves férfi betegnél endokarditisz talaján kialakult súlyos aortabillentyű-elégtelenség tett szükségessé szívsebészeti beavatkozást. A kevesebb mint...
ESETISMERTETÉS
Egy 44 éves férfi betegnél endokarditisz talaján kialakult súlyos aortabillentyű-elégtelenség tett szükségessé szívsebészeti beavatkozást. A kevesebb mint egy év alatt bekövetkezett többszöri trombotikus esemény felvetette antifoszfolipid szindróma lehetőségét. A lupus antikoaguláns pozitivitás és az említett klinikai kép ezt igazolta.
MEGBESZÉLÉS
Betegünk fiatal életkora és a biológiai műbillentyű korlátozott élettartama ellenére biológiai műbillentyű beültetése mellett döntöttünk. Választásunkat azzal indokoljuk, hogy tanulmányok bizonyítják antifoszfolipid szindrómás betegeknél a mechanikus műbillentyűvel összefüggésbe hozható thromboembolia viszonylag gyakori előfordulását. A műtétet szövődménymentesen elvégeztük, aktivált parciális thromboplastin idővel kontrollált Na-heparin korai adása mellett állítottuk be az orális antikoaguláns terápiát 3,0 INR célértékkel a műtét utáni ötödik napra. A műtét során eltávolított billentyűből korokozó nem tenyészett ki. A kórszövettani vizsgálat abakteriális endokarditiszt véleményezett, nem kizárva a korábbi fertőzést. Biológiai műbillentyű implantáció után három hónapig ajánlott aszpirin vagy K-vitamin antagonista adása, betegünk esetében viszont élethosszig tartó antikoaguláns kezelés szükséges, tekintettel rendszerbetegségére.
KÖVETKEZTETÉS
Halmozódó tromboembóliás események kapcsán gondolni kell antifoszfolipid szindrómára, mely igazolása adott esetben meghatározhatja a választható műbillentyű fajtáját. Az ajánlások legtöbbször csak általánosságban fedik le a ritka társbetegségeket, ezért a kapcsolódó szakirodalom áttekintése is szükséges az optimális, betegre szabott döntéshez.
Topics: Humans; Candy; Heparin; Mentha; Thromboplastin; Vitamin K
PubMed: 38175209
DOI: 10.1556/1046.2023.40002 -
Anti-cancer Agents in Medicinal... 2024Non-Small Cell Lung Cancer (NSCLC) is a malignancy with a significant prevalence and aggressive nature, posing a considerable challenge in terms of therapeutic...
BACKGROUND
Non-Small Cell Lung Cancer (NSCLC) is a malignancy with a significant prevalence and aggressive nature, posing a considerable challenge in terms of therapeutic interventions. Autophagy and apoptosis, two intricate cellular processes, are integral to NSCLC pathophysiology, each affecting the other through shared signaling pathways. Phytol (Phy) and α-bisabolol (Bis) have shown promise as potential anticancer agents individually, but their combined effects in NSCLC have not been extensively investigated.
OBJECTIVE
The present study was to examine the synergistic impact of Phy and Bis on NSCLC cells, particularly in the context of autophagy modulation, and to elucidate the resulting differential protein expression using LCMS/ MS analysis.
METHODS
The A549 cell lines were subjected to the patented effective concentration of Phy and Bis, and subsequently, the viability of the cells was evaluated utilizing the MTT assay. The present study utilized real-time PCR analysis to assess the expression levels of crucial apoptotic genes, specifically Bcl-2, Bax, and Caspase-9, as well as autophagy-related genes, including Beclin-1, SQSTM1, Ulk1, and LC3B. The confirmation of autophagy marker expression (Beclin-1, LC3B) and the autophagy-regulating protein SQSTM1 was achieved through the utilization of Western blot analysis. Differentially expressed proteins were found using LC-MS/MS analysis.
RESULTS
The combination of Phy and Bis demonstrated significant inhibition of NSCLC cell growth, indicating their synergistic effect. Real-time PCR analysis revealed a shift towards apoptosis, with downregulation of Bcl-2 and upregulation of Bax and Caspase-9, suggesting a shift towards apoptosis. Genes associated with autophagy regulation, including Beclin-1, SQSTM1 (p62), Ulk1, and LC3B, showed significant upregulation, indicating potential induction of autophagy. Western blot analysis confirmed increased expression of autophagy markers, such as Beclin-1 and LC3B, while the autophagy-regulating protein SQSTM1 exhibited a significant decrease. LC-MS/MS analysis revealed differential expression of 861 proteins, reflecting the modulation of cellular processes. Protein-protein interaction network analysis highlighted key proteins involved in apoptotic and autophagic pathways, including STOML2, YWHAB, POX2, B2M, CDA, CAPN2, TXN, ECHS1, PEBP1, PFN1, CDC42, TUBB1, HSPB1, PXN, FGF2, and BAG3, emphasizing their crucial roles. Additionally, PANTHER pathway analysis uncovered enriched pathways associated with the differentially expressed proteins, revealing their involvement in a diverse range of biological processes, encompassing cell signaling, metabolism, and cellular stress responses.
CONCLUSION
The combined treatment of Phy and Bis exerts a synergistic inhibitory effect on NSCLC cell growth, mediated through the interplay of apoptosis and autophagy. The differential protein expression observed, along with the identified proteins and enriched pathways, provides valuable insights into the underlying molecular mechanisms. These findings offer a foundation for further exploration of the therapeutic potential of Phy and Bis in the management of NSCLC.
Topics: Humans; Autophagy; Apoptosis; Tandem Mass Spectrometry; Cell Proliferation; Phytol; Drug Screening Assays, Antitumor; Cell Survival; Dose-Response Relationship, Drug; A549 Cells; Proteome; Chromatography, Liquid; Antineoplastic Agents, Phytogenic; Structure-Activity Relationship; Molecular Structure; Carcinoma, Non-Small-Cell Lung; Antineoplastic Agents; Drug Synergism; Lung Neoplasms; Tumor Cells, Cultured; Sesquiterpenes
PubMed: 38415491
DOI: 10.2174/0118715206289038240214102951