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Plant Cell Reports Jul 2021WRKY transcription factors are among the largest families of transcriptional regulators. In this review, their pivotal role in modulating various signal transduction... (Review)
Review
WRKY transcription factors are among the largest families of transcriptional regulators. In this review, their pivotal role in modulating various signal transduction pathways during biotic and abiotic stresses is discussed. Transcription factors (TFs) are important constituents of plant signaling pathways that define plant responses against biotic and abiotic stimuli besides playing a role in response to internal signals which coordinate different interacting partners during developmental processes. WRKY TFs, deriving their nomenclature from their signature DNA-binding sequence, represent one of the largest families of transcriptional regulators found exclusively in plants. By modulating different signal transduction pathways, these TFs contribute to various plant processes including nutrient deprivation, embryogenesis, seed and trichome development, senescence as well as other developmental and hormone-regulated processes. A growing body of research suggests transcriptional regulation of WRKY TFs in adapting plant to a variety of stressed environments. WRKY TFs can regulate diverse biological functions from receptors for pathogen triggered immunity, modulator of chromatin for specific interaction and signal transfer through a complicated network of genes. Latest discoveries illustrate the interaction of WRKY proteins with other TFs to form an integral part of signaling webs that regulate several seemingly disparate processes and defense-related genes, thus establishing their significant contributions to plant immune response. The present review starts with a brief description on the structural characteristics of WRKY TFs followed by the sections that present recent evidence on their roles in diverse biological processes in plants. We provide a comprehensive overview on regulatory crosstalks involving WRKY TFs during multiple stress responses in plants and future prospects of WRKY TFs as promising molecular diagnostics for enhancing crop improvement.
Topics: Crops, Agricultural; Multigene Family; Plant Growth Regulators; Plant Physiological Phenomena; Plant Proteins; Plants; Signal Transduction; Stress, Physiological; Transcription Factors
PubMed: 33860345
DOI: 10.1007/s00299-021-02691-8 -
Molecules (Basel, Switzerland) Mar 2018Plant secondary metabolites (SMs) are not only a useful array of natural products but also an important part of plant defense system against pathogenic attacks and... (Review)
Review
Plant secondary metabolites (SMs) are not only a useful array of natural products but also an important part of plant defense system against pathogenic attacks and environmental stresses. With remarkable biological activities, plant SMs are increasingly used as medicine ingredients and food additives for therapeutic, aromatic and culinary purposes. Various genetic, ontogenic, morphogenetic and environmental factors can influence the biosynthesis and accumulation of SMs. According to the literature reports, for example, SMs accumulation is strongly dependent on a variety of environmental factors such as light, temperature, soil water, soil fertility and salinity, and for most plants, a change in an individual factor may alter the content of SMs even if other factors remain constant. Here, we review with emphasis how each of single factors to affect the accumulation of plant secondary metabolites, and conduct a comparative analysis of relevant natural products in the stressed and unstressed plants. Expectantly, this documentary review will outline a general picture of environmental factors responsible for fluctuation in plant SMs, provide a practical way to obtain consistent quality and high quantity of bioactive compounds in vegetation, and present some suggestions for future research and development.
Topics: Biological Products; Plants; Salinity; Secondary Metabolism; Soil; Stress, Physiological; Temperature; Water
PubMed: 29584636
DOI: 10.3390/molecules23040762 -
Methods in Molecular Biology (Clifton,... 2018Programmed cell death (PCD) is a controlled mechanism that eliminates specific cells under developmental or environmental stimuli. All organisms-from bacteria to... (Review)
Review
Programmed cell death (PCD) is a controlled mechanism that eliminates specific cells under developmental or environmental stimuli. All organisms-from bacteria to multicellular eukaryotes-have the ability to induce PCD in selected cells. Although this process was first identified in plants, the interest in deciphering the signaling pathways leading to PCD strongly increased when evidence came to light that PCD may be involved in several human diseases. In plants, PCD activation ensures the correct occurrence of growth and developmental processes, among which embryogenesis and differentiation of tracheary elements. PCD is also part of the defense responses activated by plants against environmental stresses, both abiotic and biotic.This chapter gives an overview of the roles of PCD in plants as well as the problems arising in classifying different kinds of PCD according to defined biochemical and cellular markers, and in comparison with the various types of PCD occurring in mammal cells. The importance of understanding PCD signaling pathways, with their elicitors and effectors, in order to improve plant productivity and resistance to environmental stresses is also taken into consideration.
Topics: Apoptosis; Biomarkers; Disease Resistance; Energy Metabolism; Gene Expression Regulation, Plant; Plant Development; Plant Diseases; Plant Physiological Phenomena; Plants; Stress, Physiological
PubMed: 29332281
DOI: 10.1007/978-1-4939-7668-3_1 -
Annual Review of Plant Biology 2015In plant innate immunity, individual cells have the capacity to sense and respond to pathogen attack. Intracellular recognition mechanisms have evolved to intercept... (Review)
Review
In plant innate immunity, individual cells have the capacity to sense and respond to pathogen attack. Intracellular recognition mechanisms have evolved to intercept perturbations by pathogen virulence factors (effectors) early in host infection and convert it to rapid defense. One key to resistance success is a polymorphic family of intracellular nucleotide-binding/leucine-rich-repeat (NLR) receptors that detect effector interference in different parts of the cell. Effector-activated NLRs connect, in various ways, to a conserved basal resistance network in order to transcriptionally boost defense programs. Effector-triggered immunity displays remarkable robustness against pathogen disturbance, in part by employing compensatory mechanisms within the defense network. Also, the mobility of some NLRs and coordination of resistance pathways across cell compartments provides flexibility to fine-tune immune outputs. Furthermore, a number of NLRs function close to the nuclear chromatin by balancing actions of defense-repressing and defense-activating transcription factors to program cells dynamically for effective disease resistance.
Topics: Disease Resistance; Immunity, Innate; Plant Diseases; Plant Proteins; Plants; Signal Transduction; Virulence Factors
PubMed: 25494461
DOI: 10.1146/annurev-arplant-050213-040012 -
Methods in Molecular Biology (Clifton,... 2015DNA barcoding uses specific regions of DNA in order to identify species. Initiatives are taking place around the world to generate DNA barcodes for all groups of living...
DNA barcoding uses specific regions of DNA in order to identify species. Initiatives are taking place around the world to generate DNA barcodes for all groups of living organisms and to make these data publically available in order to help understand, conserve, and utilize the world's biodiversity. For land plants the core DNA barcode markers are two sections of coding regions within the chloroplast, part of the genes, rbcL and matK. In order to create high quality databases, each plant that is DNA barcoded needs to have a herbarium voucher that accompanies the rbcL and matK DNA sequences. The quality of the DNA sequences, the primers used, and trace files should also be accessible to users of the data. Multiple individuals should be DNA barcoded for each species in order to check for errors and allow for intraspecific variation. The world's herbaria provide a rich resource of already preserved and identified material and these can be used for DNA barcoding as well as by collecting fresh samples from the wild. These protocols describe the whole DNA barcoding process, from the collection of plant material from the wild or from the herbarium, how to extract and amplify the DNA, and how to check the quality of the data after sequencing.
Topics: DNA Barcoding, Taxonomic; DNA, Plant; Electrophoresis, Agar Gel; Information Systems; Plants; Polymerase Chain Reaction; Sequence Alignment; Sequence Analysis, DNA; Specimen Handling; Terminology as Topic
PubMed: 25373752
DOI: 10.1007/978-1-4939-1966-6_8 -
Chimia Nov 2022Nematodes represent the most abundant group of metazoans on earth. They utilize diverse chemicals to interact with con-specific and hetero-specific organisms, and are... (Review)
Review
Nematodes represent the most abundant group of metazoans on earth. They utilize diverse chemicals to interact with con-specific and hetero-specific organisms, and are also impacted by compounds produced by other interacting organisms. In the first part of this review we discuss how nematode-derived glycolipids modulate their behavior and development, as well as the interactions with other organisms. Furthermore, we provide a short overview about other secondary metabolites produced by nematodes that affect different life traits of free-living nematodes. In the second part of this review we discuss how different bacteria-, nematode-, and plant-derived chemicals such as volatile organic compounds, root exudates, and plant defenses regulate the interaction between entomopathogenic nematodes, their symbiotic bacteria, insect prey, predators, and plants.
Topics: Animals; Nematoda; Ecology; Plants; Volatile Organic Compounds; Bacteria
PubMed: 38069790
DOI: 10.2533/chimia.2022.945 -
Molecular Ecology Resources Jan 2016The internal transcribed spacer (ITS) of nuclear ribosomal DNA is one of the most commonly used DNA markers in plant phylogenetic and DNA barcoding analyses, and it has...
The internal transcribed spacer (ITS) of nuclear ribosomal DNA is one of the most commonly used DNA markers in plant phylogenetic and DNA barcoding analyses, and it has been recommended as a core plant DNA barcode. Despite this popularity, the universality and specificity of PCR primers for the ITS region are not satisfactory, resulting in amplification and sequencing difficulties. By thoroughly surveying and analysing the 18S, 5.8S and 26S sequences of Plantae and Fungi from GenBank, we designed new universal and plant-specific PCR primers for amplifying the whole ITS region and a part of it (ITS1 or ITS2) of plants. In silico analyses of the new and the existing ITS primers based on these highly representative data sets indicated that (i) the newly designed universal primers are suitable for over 95% of plants in most groups; and (ii) the plant-specific primers are suitable for over 85% of plants in most groups without amplification of fungi. A total of 335 samples from 219 angiosperm families, 11 gymnosperm families, 24 fern and lycophyte families, 16 moss families and 17 fungus families were used to test the performances of these primers. In vitro PCR produced similar results to those from the in silico analyses. Our new primer pairs gave PCR improvements up to 30% compared with common-used ones. The new universal ITS primers will find wide application in both plant and fungal biology, and the new plant-specific ITS primers will, by eliminating PCR amplification of nonplant templates, significantly improve the quality of ITS sequence information collections in plant molecular systematics and DNA barcoding.
Topics: DNA Barcoding, Taxonomic; DNA Primers; DNA, Plant; DNA, Ribosomal Spacer; Phylogeny; Plants; Polymerase Chain Reaction; Species Specificity
PubMed: 26084789
DOI: 10.1111/1755-0998.12438 -
Cytometry. Part a : the Journal of the... Apr 2021Flow cytometric analysis and sorting of plant mitotic chromosomes has been mastered by only a few laboratories worldwide. Yet, it has been contributing significantly to... (Review)
Review
Flow cytometric analysis and sorting of plant mitotic chromosomes has been mastered by only a few laboratories worldwide. Yet, it has been contributing significantly to progress in plant genetics, including the production of genome assemblies and the cloning of important genes. The dissection of complex genomes by flow sorting into the individual chromosomes that represent small parts of the genome reduces DNA sample complexity and streamlines projects relying on molecular and genomic techniques. Whereas flow cytometric analysis, that is, chromosome classification according to fluorescence and light scatter properties, is an integral part of any chromosome sorting project, it has rarely been used on its own due to lower resolution and sensitivity as compared to other cytogenetic methods. To perform chromosome analysis and sorting, commercially available electrostatic droplet sorters are suitable. However, in order to resolve and purify chromosomes of interest the instrument must offer high resolution of optical signals as well as stability during long runs. The challenge is thus not the instrumentation, but the adequate sample preparation. The sample must be a suspension of intact mitotic metaphase chromosomes and the protocol, which includes the induction of cell cycle synchrony, accumulation of dividing cells at metaphase, and release of undamaged chromosomes, is time consuming and laborious and needs to be performed very carefully. Moreover, in addition to fluorescent staining chromosomal DNA, the protocol may include specific labelling of DNA repeats to facilitate discrimination of particular chromosomes. This review introduces the applications of chromosome sorting in plants, and discusses in detail sample preparation, chromosome analysis and sorting to achieve the highest purity in flow-sorted fractions, and their suitability for downstream applications.
Topics: Cell Cycle; Chromosomes, Plant; Flow Cytometry; Metaphase; Plants
PubMed: 33615737
DOI: 10.1002/cyto.a.24324 -
Current Opinion in Immunology Feb 2015Intracellular immune receptors with nucleotide-binding, leucine-rich domains (NLRs) are found in both plants and animals. Compared to animals, NLR-encoding gene families... (Review)
Review
Intracellular immune receptors with nucleotide-binding, leucine-rich domains (NLRs) are found in both plants and animals. Compared to animals, NLR-encoding gene families are expanded, more prevalent and have enriched diversity in higher plants. Strong host defense triggered by the recognition of specific pathogen effectors constitutes a major part of the plant immune response that has long been exploited to breed crops for enhanced resistance. Although the first plant NLR genes were cloned about 20 years ago, their signaling mechanisms remain obscure. Here we review recent progress in plant NLR studies, focusing on their pathogen recognition, homeostasis control and potential signaling activation mechanisms.
Topics: Animals; Gene Expression Regulation, Plant; Homeostasis; Host-Pathogen Interactions; Humans; Multigene Family; Plant Proteins; Plants; Protein Interaction Domains and Motifs; Receptors, Immunologic; Signal Transduction
PubMed: 25667191
DOI: 10.1016/j.coi.2015.01.014 -
Essays in Biochemistry 2015Cytokinin is an essential plant hormone that is involved in a wide range of plant growth and developmental processes which are controlled through its signalling pathway.... (Review)
Review
Cytokinin is an essential plant hormone that is involved in a wide range of plant growth and developmental processes which are controlled through its signalling pathway. Cytokinins are a class of molecules that are N(6)-substituted adenine derivatives, such as isopentenyl adenine, and trans- and cis-zeatin, which are common in most plants. The ability to perceive and respond to cytokinin occurs through a modified bacterial two-component pathway that functions via a multi-step phosphorelay. This cytokinin signalling process is a crucial part of almost all stages of plant life, from embryo patterning to apical meristem regulation, organ development and eventually senescence. The cytokinin signalling pathway involves the co-ordination of three types of proteins: histidine kinase receptors to perceive the signal, histidine phosphotransfer proteins to relay the signal, and response regulators to provide signal output. This pathway contains both positive and negative elements that function in a complex co-ordinated manner to control cytokinin-regulated plant responses. Although much is known about how this cytokinin signal is perceived and initially regulated, there are still many avenues that need to be explored before the role of cytokinin in the control of plant processes is fully understood.
Topics: Cytokinins; Histidine Kinase; Plants; Protein Kinases; Signal Transduction
PubMed: 26374884
DOI: 10.1042/bse0580013