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International Journal of Molecular... Feb 2021Plants are constantly exposed to a wide range of potential pathogens and to protect themselves, have developed a variety of chemical and physical defense mechanisms.... (Review)
Review
Plants are constantly exposed to a wide range of potential pathogens and to protect themselves, have developed a variety of chemical and physical defense mechanisms. Callose is a β-(1,3)-D-glucan that is widely distributed in higher plants. In addition to its role in normal growth and development, callose plays an important role in plant defense. Callose is deposited between the plasma membrane and the cell wall at the site of pathogen attack, at the plasmodesmata, and on other plant tissues to slow pathogen invasion and spread. Since it was first reported more than a century ago, defense-related callose deposition has been extensively studied in a wide-spectrum of plant-pathogen systems. Over the past 20 years or so, a large number of studies have been published that address the dynamic nature of pathogen-induced callose deposition, the complex regulation of synthesis and transport of defense-related callose and associated callose synthases, and its important roles in plant defense responses. In this review, we summarize our current understanding of the regulation and function of defense-related callose deposition in plants and discuss both the progresses and future challenges in addressing this complex defense mechanism as a critical component of a plant immune system.
Topics: Gene Expression Regulation, Plant; Glucans; Glucosyltransferases; Host-Pathogen Interactions; Plant Physiological Phenomena; Plant Proteins
PubMed: 33673633
DOI: 10.3390/ijms22052393 -
Molecular Plant Jan 2016Intercellular and supracellular communications through plasmodesmata are involved in vital processes for plant development and physiological responses. Micro- and... (Review)
Review
Intercellular and supracellular communications through plasmodesmata are involved in vital processes for plant development and physiological responses. Micro- and macromolecules, including hormones, RNA, and proteins, serve as biological information vectors that traffic through the plasmodesmata between cells. Previous studies demonstrated that the plasmodesmata are elaborately regulated, whereby a long queue of multiple signaling molecules forms. However, the mechanism by which these signals are coupled or coordinated in terms of simultaneous transport in a single channel remains a puzzle. In the last few years, several phytohormones that could function as both non-cell-autonomous signals and plasmodesmal regulators have been disclosed. Plasmodesmal regulators such as auxin, salicylic acid, reactive oxygen species, gibberellic acids, chitin, and jasmonic acid could regulate intercellular trafficking by adjusting plasmodesmal permeability. Here, callose, along with β-glucan synthase and β-glucanase, plays a critical role in regulating plasmodesmal permeability. Interestingly, most of the previously identified regulators are capable of diffusing through the plasmodesmata. Given the small sizes of these molecules, the plasmodesmata are prominent intercellular channels that allow diffusion-based movement of those signaling molecules. Obviously, intercellular communication is under the control of a major mechanism, named a feedback loop, at the plasmodesmata, which mediates complicated biological behaviors. Prospective research on the mechanism of coupling micromolecules at the plasmodesmata for developmental signaling and nutrient provision will help us to understand how plants coordinate their development and photosynthetic assimilation, which is important for agriculture.
Topics: Cell Communication; Glucans; Glucosyltransferases; Plant Growth Regulators; Plants; Plasmodesmata; Signal Transduction
PubMed: 26384246
DOI: 10.1016/j.molp.2015.08.015 -
Journal of Experimental Botany Jan 2020Plasmodesmata traverse cell walls, generating connections between neighboring cells. They allow intercellular movement of molecules such as transcription factors,... (Review)
Review
Plasmodesmata traverse cell walls, generating connections between neighboring cells. They allow intercellular movement of molecules such as transcription factors, hormones, and sugars, and thus create a symplasmic continuity within a tissue. One important factor that determines plasmodesmal permeability is their aperture, which is regulated during developmental and physiological processes. Regulation of aperture has been shown to affect developmental events such as vascular differentiation in the root, initiation of lateral roots, or transition to flowering. Extensive research has unraveled molecular factors involved in the regulation of plasmodesmal permeability. Nevertheless, many plant developmental processes appear to involve feedbacks mediated by mechanical forces, raising the question of whether mechanical forces and plasmodesmal permeability affect each other. Here, we review experimental data on how one of these forces, turgor pressure, and plasmodesmal permeability may mutually influence each other during plant development, and we discuss the questions raised by these data. Addressing such questions will improve our knowledge of how cellular patterns emerge during development, shedding light on the evolution of complex multicellular plants.
Topics: Hydrostatic Pressure; Osmotic Pressure; Permeability; Plant Development; Plasmodesmata
PubMed: 31563945
DOI: 10.1093/jxb/erz434 -
The New Phytologist Jul 2021Endodormancy and the related chilling requirement synchronize the seasonal development of trees from the boreal and temperate regions under the climatic conditions... (Review)
Review
Endodormancy and the related chilling requirement synchronize the seasonal development of trees from the boreal and temperate regions under the climatic conditions prevailing at their native growing sites. The phenomenon of endodormancy has been known at the whole-plant level for 100 years, and in the last couple of decades, insights into the physiological and molecular basis of endodormancy and its release have also been obtained. Intriguingly, recent studies have shown experimentally that subtropical trees also show endodormancy and a chilling requirement. Motivated by the climatic differences between the subtropical and more northern zones, here we address the similarities and differences in endodormancy between trees growing in the subtropical zone and those growing in more northern zones.
Topics: Climate Change; Seasons; Trees
PubMed: 33811341
DOI: 10.1111/nph.17382 -
Molecular Plant-microbe Interactions :... Jan 2020Plasmodesmata (PD) are essential for intercellular trafficking of molecules required for plant life, from small molecules like sugars and ions to macromolecules... (Review)
Review
Plasmodesmata (PD) are essential for intercellular trafficking of molecules required for plant life, from small molecules like sugars and ions to macromolecules including proteins and RNA molecules that act as signals to regulate plant development and defense. As obligate intracellular pathogens, plant viruses have evolved to manipulate this communication system to facilitate the initial cell-to-cell and eventual systemic spread in their plant hosts. There has been considerable interest in how viruses manipulate the PD that connect the protoplasts of neighboring cells, and viruses have yielded invaluable tools for probing the structure and function of PD. With recent advances in biochemistry and imaging, we have gained new insights into the composition and structure of PD in the presence and absence of viruses. Here, we first discuss viral strategies for manipulating PD for their intercellular movement and examine how this has shed light on our understanding of native PD function. We then address the controversial role of the cytoskeleton in trafficking to and through PD. Finally, we address how viruses could alter PD structure and consider possible mechanisms of the phenomenon described as 'gating'. This discussion supports the significance of virus research in elucidating the properties of PD, these persistently enigmatic plant organelles.
Topics: Cytoskeleton; Plant Development; Plant Viruses; Plants; Plasmodesmata; Protein Transport; Signal Transduction
PubMed: 31715107
DOI: 10.1094/MPMI-07-19-0212-FI -
Frontiers in Plant Science 2021The ability to develop secondary (post-cytokinetic) plasmodesmata (PD) is an important evolutionary advantage that helps in creating symplastic domains within the plant...
The ability to develop secondary (post-cytokinetic) plasmodesmata (PD) is an important evolutionary advantage that helps in creating symplastic domains within the plant body. Developmental regulation of secondary PD formation is not completely understood. In flowering plants, secondary PD occur exclusively between cells from different lineages, e.g., at the L1/L2 interface within shoot apices, or between leaf epidermis (L1-derivative), and mesophyll (L2-derivative). However, the highest numbers of secondary PD occur in the minor veins of leaf between bundle sheath cells and phloem companion cells in a group of plant species designated "symplastic" phloem loaders, as opposed to "apoplastic" loaders. This poses a question of whether secondary PD formation is upregulated in general in symplastic loaders. Distribution of PD in leaves and in shoot apices of two symplastic phloem loaders, and , was compared with that in two apoplastic loaders, (potato) and (barley), using immunolabeling of the PD-specific proteins and transmission electron microscopy (TEM), respectively. Single-cell sampling was performed to correlate sugar allocation between leaf epidermis and mesophyll to PD abundance. Although the distribution of PD in the leaf lamina (except within the vascular tissues) and in the meristem layers was similar in all species examined, far fewer PD were found at the epidermis/epidermis and mesophyll/epidermis boundaries in apoplastic loaders compared to symplastic loaders. In the latter, the leaf epidermis accumulated sugar, suggesting sugar import from the mesophyll PD. Thus, leaf epidermis and mesophyll might represent a single symplastic domain in and .
PubMed: 34394148
DOI: 10.3389/fpls.2021.695415 -
Plant, Cell & Environment Feb 2023Cell walls are essential for plant growth and development, providing support and protection from external environments. Callose is a glucan that accumulates in... (Review)
Review
Cell walls are essential for plant growth and development, providing support and protection from external environments. Callose is a glucan that accumulates in specialized cell wall microdomains including around intercellular pores called plasmodesmata. Despite representing a small percentage of the cell wall (~0.3% in the model plant Arabidopsis thaliana), callose accumulation regulates important biological processes such as phloem and pollen development, cell division, organ formation, responses to pathogenic invasion and to changes in nutrients and toxic metals in the soil. Callose accumulation modifies cell wall properties and restricts plasmodesmata aperture, affecting the transport of signaling proteins and RNA molecules that regulate plant developmental and environmental responses. Although the importance of callose, at and outside plasmodesmata cell walls, is widely recognized, the underlying mechanisms controlling changes in its synthesis and degradation are still unresolved. In this review, we explore the most recent literature addressing callose metabolism with a focus on the molecular factors affecting callose accumulation in response to mutualistic symbionts and pathogenic elicitors. We discuss commonalities in the signaling pathways, identify research gaps and highlight opportunities to target callose in the improvement of plant responses to beneficial versus pathogenic microbes.
Topics: Plasmodesmata; Plants; Arabidopsis; Glucans; Cell Wall
PubMed: 36478232
DOI: 10.1111/pce.14510 -
Plants (Basel, Switzerland) Aug 2023In order to adapt to sessile life and terrestrial environments, vascular plants have developed highly sophisticated cells to transport photosynthetic products and... (Review)
Review
In order to adapt to sessile life and terrestrial environments, vascular plants have developed highly sophisticated cells to transport photosynthetic products and developmental signals. Of these, two distinct cell types (i.e., the sieve element (SE) and companion cell) are arranged in precise positions, thus ensuring effective transport. During SE differentiation, most of the cellular components are heavily modified or even eliminated. This peculiar differentiation implies the selective disintegration of the nucleus (i.e., enucleation) and the loss of cellular translational capacity. However, some cellular components necessary for transport (e.g., plasmalemma) are retained and specific phloem proteins (P-proteins) appear. Likewise, MYB (i.e., ) and NAC (i.e., and ) transcription factors (TFs) and OCTOPUS proteins play a notable role in SE differentiation. The maturing SEs become heavily dependent on neighboring non-conducting companion cells, to which they are connected by plasmodesmata through which only 20-70 kDa compounds seem to be able to pass. The study of sieve tube proteins still has many gaps. However, the development of a protocol to isolate proteins that are free from any contaminating proteins has constituted an important advance. This review considers the very detailed current state of knowledge of both bound and soluble sap proteins, as well as the role played by the companion cells in their presence. Phloem proteins travel long distances by combining two modes: non-selective transport via bulk flow and selective regulated movement. One of the goals of this study is to discover how the protein content of the sieve tube is controlled. The majority of questions and approaches about the heterogeneity of phloem sap will be clarified once the morphology and physiology of the plasmodesmata have been investigated in depth. Finally, the retention of specific proteins inside an SE is an aspect that should not be forgotten.
PubMed: 37687278
DOI: 10.3390/plants12173033 -
Journal of Experimental Botany Dec 2017The intercellular transport of molecules through membranous channels that traverse the cell walls-so-called plasmodesmata-is of fundamental importance for plant... (Review)
Review
The intercellular transport of molecules through membranous channels that traverse the cell walls-so-called plasmodesmata-is of fundamental importance for plant development. Regulation of plasmodesmata aperture (and transport capacity) is mediated by changes in the flanking cell walls, mainly via the synthesis/degradation (turnover) of the (1,3)-β-glucan polymer callose. The role of callose in organ development and in plant environmental responses is well recognized, but detailed understanding of the mechanisms regulating its accumulation and its effects on the structure and permeability of the channels is still missing. We compiled information on the molecular components and signalling pathways involved in callose turnover at plasmodesmata and, more generally, on the structural and mechanical properties of (1,3)-β-glucan polymers in cell walls. Based on this revision, we propose models integrating callose, cell walls, and the regulation of plasmodesmata structure and intercellular communication. We also highlight new tools and interdisciplinary approaches that can be applied to gain further insight into the effects of modifying callose in cell walls and its consequences for intercellular signalling.
Topics: Biological Transport; Cell Wall; Glucans; Models, Biological; Plant Physiological Phenomena; Plasmodesmata; Signal Transduction
PubMed: 29040641
DOI: 10.1093/jxb/erx337 -
Plants (Basel, Switzerland) Jan 2019Sieve pores of the sieve plates connect neighboring sieve elements to form the conducting sieve tubes of the phloem. Sieve pores are critical for phloem function. From... (Review)
Review
Sieve pores of the sieve plates connect neighboring sieve elements to form the conducting sieve tubes of the phloem. Sieve pores are critical for phloem function. From the 1950s onwards, when electron microscopes became increasingly available, the study of their formation had been a pillar of phloem research. More recent work on sieve elements instead has largely focused on sieve tube hydraulics, phylogeny, and eco-physiology. Additionally, advanced molecular and genetic tools available for the model species helped decipher several key regulatory mechanisms of early phloem development. Yet, the downstream differentiation processes which form the conductive sieve tube are still largely unknown, and our understanding of sieve pore formation has only moderately progressed. Here, we summarize our current knowledge on sieve pore formation and present relevant recent advances in related fields such as sieve element evolution, physiology, and plasmodesmata formation.
PubMed: 30678196
DOI: 10.3390/plants8020025