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Trends in Plant Science Feb 2018Sexual reproduction relies on the successful fusion of the sperm and egg cell. Despite the vast differences between plants and animals, there are similarities at a... (Review)
Review
Sexual reproduction relies on the successful fusion of the sperm and egg cell. Despite the vast differences between plants and animals, there are similarities at a molecular level between plant and animal reproduction. While the molecular basis of fertilization has been extensively studied in plants, the process of capacitation has received little attention until recently. Recent research has started to uncover the molecular basis of plant capacitation. Furthermore, recent studies suggest that the key molecules in plants and animal fertilization are functionally conserved. Here, we review new insights for our understanding of capacitation of pollen tube and fertilization in plants and also propose that there are commonalities in the process of sexual reproduction between plants and animals.
Topics: Animals; Arabidopsis; Female; Fertilization; Germ Cells, Plant; Male; Pollen Tube; Sperm-Ovum Interactions
PubMed: 29170007
DOI: 10.1016/j.tplants.2017.10.006 -
Plant Physiology Jan 2017Pollen tube guidance in flowering plants is a unique and critical process for successful sexual reproduction. The pollen tube that grows from pollen, which is the male... (Review)
Review
Pollen tube guidance in flowering plants is a unique and critical process for successful sexual reproduction. The pollen tube that grows from pollen, which is the male gametophyte, precisely navigates to the embryo sac, which is the female gametophyte, within the pistil. Recent advances have clarified the molecular framework of gametophytic pollen tube guidance. Multiple species-specific attractant peptides are secreted from synergid cells, the proper development and function of which are regulated by female gametes. Multiple receptor-like kinases on the pollen tube tip are involved in sensing species-specific attractant peptides. In this Update article, recent progress in our understanding of the mechanism of gametophytic pollen tube guidance is reviewed, including attraction by synergid cells, control of pollen tube guidance by female gametes, and directional growth of the pollen tube by directional cue sensing. Future directions in the study of pollen tube guidance also are discussed.
Topics: Models, Biological; Ovule; Peptides; Pollen Tube
PubMed: 27920159
DOI: 10.1104/pp.16.01571 -
Plant Cell Reports Dec 2023VAMP726/VAMP725 and SYP131 can form a part of a SNARE complex to mediate vesicle secretion at the pollen tube apex. Secretory vesicle fusion with the plasma membrane of...
VAMP726/VAMP725 and SYP131 can form a part of a SNARE complex to mediate vesicle secretion at the pollen tube apex. Secretory vesicle fusion with the plasma membrane of the pollen tube tip is a key step in pollen tube growth. Membrane fusion was mediated by SNAREs. However, little is known about the composition and function of the SNARE complex during pollen tube tip growth. In this study, we constructed a double mutant vamp725 vamp726 via CRISPR‒Cas9. Fluorescence labeling combined with microscopic observation, luciferase complementation imaging, co-immunoprecipitation and GST pull-down were applied in the study. We show that double mutation of the R-SNAREs VAMP726 and VAMP725 significantly inhibits pollen tube growth in Arabidopsis and slows vesicle exocytosis at the apex of the pollen tube. GFP-VAMP726 and VAMP725-GFP localize mainly to secretory vesicles and the plasma membrane at the apex of the pollen tube. In addition, fluorescence recovery after photobleaching (FRAP) experiments showed that mCherry-VAMP726 colocalizes with Qa-SNARE SYP131 in the central region of the pollen tube apical plasma membrane. Furthermore, we found that VAMP726 and VAMP725 can interact with the SYP131. Based on these results, we suggest that VAMP726/VAMP725 and SYP131 can form a part of a SNARE complex to mediate vesicle secretion at the pollen tube apex, and vesicle secretion may mainly occur at the central region of the pollen tube apical plasma membrane.
Topics: Arabidopsis; Pollen Tube; Arabidopsis Proteins; Cell Membrane; SNARE Proteins
PubMed: 37805949
DOI: 10.1007/s00299-023-03075-w -
Plant Reproduction Mar 2022The pollen tube is ideal for studying cell polar growth, and observing the ultrastructure of the pollen tube tip using transmission electron microscopy (TEM) is the...
The pollen tube is ideal for studying cell polar growth, and observing the ultrastructure of the pollen tube tip using transmission electron microscopy (TEM) is the primary method for studying pollen tube growth. The preparation of ultrathin sections of the pollen tube tip sample is important for its successful microscopic observation. The direction of pollen tube growth in vitro is irregular, and it is difficult to dissect the tip of the pollen tube during ultrathin sectioning. Here, we used two methods to efficiently obtain an ultrathin section of the pollen tube tip of Pyrus. In the first method, laser micro-cutting was used to obtain the pollen tube tip, followed by ultrathin sectioning. In the other method, the pollen tubes were cultured in the same growth direction, followed by ultrathin sectioning. Ultrathin sections, which were observed via TEM, showed typical characteristics of the pollen tube tip, such as dense vesicles, numerous mitochondria, and secretory vesicles of the Golgi. We concluded that these two methods are effective in pollen tube tip sample preparation for ultrathin sectioning and provide the foundation for observing the ultrastructure of pollen tube tips.
Topics: Pollen Tube; Pyrus; Secretory Vesicles
PubMed: 34731307
DOI: 10.1007/s00497-021-00433-0 -
Journal of Integrative Plant Biology Jul 2023Pollen tube polar growth is a key physiological activity for angiosperms to complete double fertilization, which is highly dependent on the transport of polar substances...
Pollen tube polar growth is a key physiological activity for angiosperms to complete double fertilization, which is highly dependent on the transport of polar substances mediated by secretory vesicles. The exocyst and Sec1/Munc18 (SM) proteins are involved in the regulation of the tethering and fusion of vesicles and plasma membranes, but the molecular mechanism by which they regulate pollen tube polar growth is still unclear. In this study, we found that loss of function of SEC1A, a member of the SM protein family in Arabidopsis thaliana, resulted in reducing pollen tube growth and a significant increase in pollen tube width. SEC1A was diffusely distributed in the pollen tube cytoplasm, and was more concentrated at the tip of the pollen tube. Through co-immunoprecipitation-mass spectrometry screening, protein interaction analysis and in vivo microscopy, we found that SEC1A interacted with the exocyst subunit SEC6, and they mutually affected the distribution and secretion rate at the tip of the pollen tube. Meanwhile, the functional loss of SEC1A and SEC6 significantly affected the distribution of the SNARE (soluble N-ethylmaleimide-sensitive factor attachment protein receptor) complex member SYP125 at the tip of the pollen tube, and led to the disorder of pollen tube cell wall components. Genetic analysis revealed that the pollen tube-related phenotype of the sec1a sec6 double mutant was significantly enhanced compared with their respective single mutants. Therefore, we speculated that SEC1A and SEC6 cooperatively regulate the fusion of secretory vesicles and plasma membranes in pollen tubes, thereby affecting the length and the width of pollen tubes.
Topics: Arabidopsis; Arabidopsis Proteins; Cell Cycle; Cell Membrane; Pollen Tube
PubMed: 36951316
DOI: 10.1111/jipb.13486 -
International Journal of Molecular... Nov 2021In angiosperms, double fertilization requires pollen tubes to transport non-motile sperm to distant egg cells housed in a specialized female structure known as the... (Review)
Review
In angiosperms, double fertilization requires pollen tubes to transport non-motile sperm to distant egg cells housed in a specialized female structure known as the pistil, mediating the ultimate fusion between male and female gametes. During this journey, the pollen tube encounters numerous physical barriers that must be mechanically circumvented, including the penetration of the stigmatic papillae, style, transmitting tract, and synergid cells as well as the ultimate fusion of sperm cells to the egg or central cell. Additionally, the pollen tube must maintain structural integrity in these compact environments, while responding to positional guidance cues that lead the pollen tube to its destination. Here, we discuss the nature of these physical barriers as well as efforts to genetically and cellularly identify the factors that allow pollen tubes to successfully, specifically, and quickly circumnavigate them.
Topics: Cell Communication; Flowers; Pollen Tube; Pollination
PubMed: 34830110
DOI: 10.3390/ijms222212230 -
Journal of Experimental Botany Aug 2015Flowering seed plants (angiosperms) have evolved unique ways to protect their gametes from pathogen attack and from drying out. The female gametes (egg and central cell)... (Review)
Review
Flowering seed plants (angiosperms) have evolved unique ways to protect their gametes from pathogen attack and from drying out. The female gametes (egg and central cell) are deeply embedded in the maternal tissues of the ovule inside the ovary, while the male gametes (sperm cells) are enclosed in the vegetative pollen tube cell. After germination of the pollen tube at the surface of papilla cells of the stigma the two immobile sperm cells are transported deep inside the sporophytic maternal tissues to be released inside the ovule for double fertilization. Angiosperms have evolved a number of hurdles along the pollen tube journey to prevent inbreeding and fertilization by alien sperm cells, and to maximize reproductive success. These pre-zygotic hybridization barriers require intensive communication between the male and female reproductive cells and the necessity to distinguish self from non-self interaction partners. General molecules such as nitric oxide (NO) or gamma-aminobutyric acid (GABA) therefore appear to play only a minor role in these species-specific communication events. The past 20 years have shown that highly polymorphic peptides play a leading role in all communication steps along the pollen tube pathway and fertilization. Here we review our current understanding of the role of peptides during reproduction with a focus on peptide signalling during self-incompatibility, pollen tube growth and guidance as well as sperm reception and gamete activation.
Topics: Magnoliopsida; Peptides; Plant Proteins; Pollen Tube; Reproduction; Self-Incompatibility in Flowering Plants; Signal Transduction
PubMed: 26068467
DOI: 10.1093/jxb/erv275 -
Journal of Integrative Plant Biology Sep 2023Pollen tube growth is essential for successful double fertilization, which is critical for grain yield in crop plants. Rapid alkalinization factors (RALFs) function as...
Pollen tube growth is essential for successful double fertilization, which is critical for grain yield in crop plants. Rapid alkalinization factors (RALFs) function as ligands for signal transduction during fertilization. However, functional studies on RALF in monocot plants are lacking. Herein, we functionally characterized two pollen-specific RALFs in rice (Oryza sativa) using multiple clustered regularly interspaced palindromic repeats (CRISPR)/CRISPR-associated protein 9-induced loss-of-function mutants, peptide treatment, expression analyses, and tag reporter lines. Among the 41 RALF members in rice, OsRALF17 was specifically expressed at the highest level in pollen and pollen tubes. Exogenously applied OsRALF17 or OsRALF19 peptide inhibited pollen tube germination and elongation at high concentrations but enhanced tube elongation at low concentrations, indicating growth regulation. Double mutants of OsRALF17 and OsRALF19 (ralf17/19) exhibited almost full male sterility with defects in pollen hydration, germination, and tube elongation, which was partially recovered by exogenous treatment with OsRALF17 peptide. This study revealed that two partially functionally redundant OsRALF17 and OsRALF19 bind to Oryza sativa male-gene transfer defective 2 (OsMTD2) and transmit reactive oxygen species signals for pollen tube germination and integrity maintenance in rice. Transcriptomic analysis confirmed their common downstream genes, in osmtd2 and ralf17/19. This study provides new insights into the role of RALF, expanding our knowledge of the biological role of RALF in regulating rice fertilization.
Topics: Pollen Tube; Oryza; Pollen; Signal Transduction; Peptides
PubMed: 37195059
DOI: 10.1111/jipb.13508 -
Annual Review of Plant Biology 2015During sexual reproduction of flowering plants, pollen tube guidance by pistil tissue is critical for the delivery of nonmotile sperm cells to female gametes. Multistep... (Review)
Review
During sexual reproduction of flowering plants, pollen tube guidance by pistil tissue is critical for the delivery of nonmotile sperm cells to female gametes. Multistep controls of pollen tube guidance can be divided into two phases: preovular guidance and ovular guidance. During preovular guidance, various female molecules, including stimulants for pollen germination and pollen tube growth, are provided to support tube growth toward the ovary, where the ovules are located. After entering the ovary, pollen tubes receive directional cues from their respective target ovules, including attractant peptides for precise, species-preferential attraction. Successful pollen tube guidance in the pistil requires not only nutritional and directional controls but also competency controls to make pollen tubes responsive to guidance cues, regulation to terminate growth once a pollen tube arrives at the target, and strategies to stop ovular attraction depending on the fertilization of female gametes.
Topics: Fertilization; Magnoliopsida; Ovule; Peptides; Pollen Tube; Pollination
PubMed: 25621518
DOI: 10.1146/annurev-arplant-043014-115635 -
Annual Review of Plant Biology Jun 2021Pollen-pistil interactions serve as important prezygotic reproductive barriers that play a critical role in mate selection in plants. Here, we highlight recent progress...
Pollen-pistil interactions serve as important prezygotic reproductive barriers that play a critical role in mate selection in plants. Here, we highlight recent progress toward understanding the molecular basis of pollen-pistil interactions as reproductive isolating barriers. These barriers can be active systems of pollen rejection, or they can result from a mismatch of required male and female factors. In some cases, the barriers are mechanistically linked to self-incompatibility systems, while others represent completely independent processes. Pollen-pistil reproductive barriers can act as soon as pollen is deposited on a stigma, where penetration of heterospecific pollen tubes is blocked by the stigma papillae. As pollen tubes extend, the female transmitting tissue can selectively limit growth by producing cell wall-modifying enzymes and cytotoxins that interact with the growing pollen tube. At ovules, differential pollen tube attraction and inhibition of sperm cell release can act as barriers to heterospecific pollen tubes.
Topics: Flowers; Pollen; Pollen Tube; Pollination; Reproduction
PubMed: 34143652
DOI: 10.1146/annurev-arplant-080620-102159