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The Science of the Total Environment Jan 2023The acute toxic effects of pristine and aged polystyrene (P-PS and A-PS) and their leaching solutions (L-PS) on microalgae Skeletonema costatum were investigated by...
The acute toxic effects of pristine and aged polystyrene (P-PS and A-PS) and their leaching solutions (L-PS) on microalgae Skeletonema costatum were investigated by measuring algal density and growth inhibition rate (IR), chlorophyll concentration and photosynthetic efficiency (Fv/Fm) over 96 h. Total protein (TP), superoxide dismutase (SOD), catalase (CAT) and malondialdehyde (MDA) were measured to analyze the oxidative damage to microalgae by microplastics and their leachates. Hydrodynamic diameter of microplastics in seawater, FITR and SEM images were used to study the changes of polystyrene during aging. The interaction of algae cell with microplastics and the cellular ultrastructure changes of cells were analyzed combined with electron microscopy for a comprehensive and systematic understanding on the mechanisms of microplastic toxicity to microalgae. Both high concentration and small size of PS had significant inhibitory effect on the growth of microalgae, and the inhibitory effect was greater with increasing exposure time. The inhibition effect of aged microplastics was more obvious, which was speculated to be caused by the synergistic effect of aged PS itself and leaching solution. The negative effect of leaching solution on microalgae was due to the release of some additives during the aging process. The content of MDA reached the highest value of 54.41 nmol/mgprot in 1.0 μm 50 mg/L A-PS treatment group, and A-PS were found to be more prone to heterogeneous aggregation with algae cells by SEM.
Topics: Microalgae; Polystyrenes; Microplastics; Plastics; Diatoms; Water Pollutants, Chemical
PubMed: 36283517
DOI: 10.1016/j.scitotenv.2022.159614 -
Ecotoxicology and Environmental Safety Jun 2022The use of polystyrene micro and nanoplastics in cosmetics and personal care products continues to grow every day. The harmful effects of their biological accumulation...
The use of polystyrene micro and nanoplastics in cosmetics and personal care products continues to grow every day. The harmful effects of their biological accumulation in organisms of all trophic levels including humans have been reported by several studies. While we have accumulating evidence on the impact of nanoplastics on different organ systems in humans, only a handful of reports on the impact of polystyrene nanoplastics upon direct contact with the immune system at the cellular level are avialable. The present study offers significant evidence on the cell-specific harmful impact of sulfate-modified nanoplastics (S-NPs) on human macrophages. Here we report that exposure of human macrophages to S-NPs (100 µg/mL) stimulated the accumulation of lipids droplets (LDs) in the cytoplasm resulting in the differentiation of macrophages into foam cells. The observed effect was specific for human and murine macrophages but not for other cell types, especially human keratinocytes, liver, and lung cell models. Furthermore, we found that S-NPs mediated LDs accumulation in human macrophages was accompanied by acute mitochondrial oxidative stress. The accumulated LDs were further delivered and accumulated into lysosomes leading to impaired lysosomal clearance. In conclusion, our study reveals that exposure to polystyrene nanoplastics stabilized with anionic surfactants can be a potent stimulus for dysregulation of lipid metabolism and macrophage foam cell formation, a characteristic feature observed during atherosclerosis posing a serious threat to human health.
Topics: Animals; Atherosclerosis; Humans; Lipid Metabolism; Lysosomes; Macrophages; Mice; Microplastics; Nanoparticles; Polystyrenes
PubMed: 35561548
DOI: 10.1016/j.ecoenv.2022.113612 -
Journal of Hazardous Materials Jul 2023In the era of plastic use, organisms are constantly exposed to polystyrene particles (PS-Ps). PS-Ps accumulated in living organisms exert negative effects on the body,...
In the era of plastic use, organisms are constantly exposed to polystyrene particles (PS-Ps). PS-Ps accumulated in living organisms exert negative effects on the body, although studies evaluating their effects on brain development are scarce. In this study, the effects of PS-Ps on nervous system development were investigated using cultured primary cortical neurons and mice exposed to PS-Ps at different stages of brain development. The gene expression associated with brain development was downregulated in embryonic brains following PS-Ps exposure, and Gabra2 expression decreased in the embryonic and adult mice exposed to PS-Ps. Additionally, offspring of PS-Ps-treated dams exhibited signs of anxiety- and depression-like behavior, and abnormal social behavior. We propose that PS-Ps accumulation in the brain disrupts brain development and behavior in mice. This study provides novel information regarding PS-Ps toxicity and its harmful effects on neural development and behavior in mammals.
Topics: Animals; Mice; Polystyrenes; Depression; Water Pollutants, Chemical; Anxiety; Social Behavior; Nanoparticles; Mammals
PubMed: 37130475
DOI: 10.1016/j.jhazmat.2023.131465 -
Environmental Pollution (Barking, Essex... Feb 2024The extensive utilization and inadequate handling of plastics have resulted in severe environmental ramifications. In particular, plastics composed solely of a... (Review)
Review
The extensive utilization and inadequate handling of plastics have resulted in severe environmental ramifications. In particular, plastics composed solely of a carbon-carbon (C-C) backbone exhibit limited degradation due to the absence of hydrolyzable functional groups. Plastics with enduring longevity in the natural environment are susceptible to environmental factors and their intrinsic properties, subsequently undergoing a series of aging processes that culminate in biodegradation. This article focuses on polystyrene (PS), which constitutes 20% of total plastic waste, as a case study. Initially, the application of PS in life and the impacts it poses are introduced. Following that, the key factors influencing the aging of PS are discussed, primarily encompassing its properties (e.g., surface characteristics, additives) and environmental factors (e.g., water matrices, biofilms). Lastly, an overview of microbial degradation of PS is provided, including potential microorganisms involved in PS degradation (bacteria, fungi, algae, and insects), four processes of microbial degradation (colonization, bio-fragmentation, assimilation, and mineralization), and potential mechanisms of microbial degradation. This study provides a comprehensive understanding of the multifaceted influences affecting the aging and biodegradation mechanisms of PS, thereby contributing valuable insights for the future management of plastic pollution.
Topics: Polystyrenes; Plastics; Biodegradation, Environmental; Carbon
PubMed: 38016589
DOI: 10.1016/j.envpol.2023.123034 -
Advanced Materials (Deerfield Beach,... Jul 2022Tissue-culture-ware polystyrene is the gold standard for in vitro cell culture. While microengineering techniques can create advanced cell microenvironments in...
Tissue-culture-ware polystyrene is the gold standard for in vitro cell culture. While microengineering techniques can create advanced cell microenvironments in polystyrene, they require specialized equipment and reagents, which hinder their accessibility for most biological researchers. An economical and easily accessible method is developed and validated for fabricating microstructures directly in polystyrene with sizes approaching subcellular dimensions while requiring minimal processing time. The process involves deep ultraviolet irradiation through a shadow mask or ink pattern using inexpensive, handheld devices followed by selective chemical development with common reagents to generate micropatterns with depths/heights between 5 and 10 µm, which can be used to guide cell behavior. The remarkable straightforwardness of the process enables this class of microengineering techniques to be broadly accessible to diverse research communities.
Topics: Cell Culture Techniques; Plastics; Polystyrenes; Printing; Ultraviolet Rays
PubMed: 35358334
DOI: 10.1002/adma.202200687 -
Pharmacology Research & Perspectives Aug 2021This study explored the binding of 28 drugs, which were selected based on frequency of concomitant use and chemical properties, to sevelamer and polystyrene sulfonate in...
This study explored the binding of 28 drugs, which were selected based on frequency of concomitant use and chemical properties, to sevelamer and polystyrene sulfonate in vitro. The relative binding was determined by dissolving the investigated drugs alone (=control), together with 800 mg of sevelamer and 15 g of polystyrene sulfonate at different pH levels (1.5, 5.5, and 7.4), respectively. After incubation at 37℃ and shaking for 60 min, the solutions were diluted and centrifuged, and the drug concentrations were quantified with validated analytical assays. The binding assays were performed in threefold. The mean relative binding (MRB) at each pH level was calculated, with a MRB >20% for at least one pH level to be considered as relevant binding. Fourteen and 23 potentially new binding interactions were identified with sevelamer and polystyrene sulfonate, respectively. These potentially new binding interactions have to be studied in vivo to assess their clinical relevance.
Topics: Hydrogen-Ion Concentration; Pharmaceutical Preparations; Polystyrenes; Sevelamer
PubMed: 34302439
DOI: 10.1002/prp2.834 -
Environmental Science and Pollution... Mar 2023Health hazards associated with microplastics (MPs) remain largely unknown, and the effects of aged MPs, one of their persistent forms, are poorly characterized. Male ICR...
Health hazards associated with microplastics (MPs) remain largely unknown, and the effects of aged MPs, one of their persistent forms, are poorly characterized. Male ICR mice were intratracheally instilled with 0.01 and 1 mg/day pristine and ultraviolet (UV)-aged polystyrene microplastics (PS and APS) with an average diameter of 4 - 5 μm daily for 1 week. UV irradiation caused the PS to have a rough surface, become fragmented, and increase their carbonyl groups. Both PS and APS caused structural damage to the mouse gut, liver, spleen, and testis. Inflammatory infiltration in liver, swollen and congested gut, and loose spleen globules, as well as the loose interstitium of the seminiferous tubules in testis were found in 1 mg/day APS group. Increases in serum alanine aminotransferase and immunoglobulin A levels in 1 mg/day APS group (p < 0.05) demonstrated that APS exposure could induce greater liver and spleen functional damage than PS. Meanwhile, triglyceride and total cholesterol levels in liver were enhanced in 1 mg/day APS group (p < 0.05). Superoxide dismutase and glutathione contents in 0.01 and 1 mg/day APS groups significantly decreased (p < 0.05), which suggesting that PS and APS could interfere with the antioxidant capacity in mice. Nuclear factor erythroid 2-related factor 2 (Nrf2) and heme oxygenase-1 (HO-1) levels in the PS and APS groups showed significant increases in the liver and testis (p < 0.05), and a significant decrease in the spleen (p < 0.05), which were analyzed to get a first survey for Nrf2/HO-1-mediated tissue-specific defense mechanisms. In conclusion, acute exposure to PS and APS induced potential metabolic disorders, and APS could produce more serious immune damage and reproductive toxicity. These findings provide new insights in health risk assessment of aged MPs.
Topics: Mice; Male; Animals; Polystyrenes; Microplastics; Plastics; NF-E2-Related Factor 2; Organ Specificity; Mice, Inbred ICR; Defense Mechanisms
PubMed: 36602732
DOI: 10.1007/s11356-022-24918-1 -
Chemosphere Nov 2022Microorganisms play an important role in biogeochemical cycles, and are inevitably found associated with plastic debris. The interplay between microbes and plastics may...
Microorganisms play an important role in biogeochemical cycles, and are inevitably found associated with plastic debris. The interplay between microbes and plastics may change the characteristics of certain plastics over time and drive the environmental fate of plastics. In this study, we evaluated interactions of bacteria with nano- and microplastics. Here, polystyrene (PS) polymer particles of various diameters, specifically 60, 220, 430, 700, 1040, 1700, and 2260 nm, were used as the plastics. Escherichia coli (E. coli, gram-negative) and Bacillus sp. (gram-positive) were chosen as model bacteria. The effects of nano- and microPS particles on E. coli and Bacillus sp. cells were investigated by measuring the growth and viability of the cells in laboratory-scale flasks and their generation of reactive oxygen species (ROS) upon their exposure to these particles of 100 mg/L. The particles inhibited the growth and viability of both types of bacterial cells, but their inhibitory effects varied depending on the diameter of PS particle. The 60-nm-diameter PS particles were visually observed to enter the cells as well as accumulate on their surfaces and enhanced ROS generation of the cells. Unexpectedly, the 1040-nm-diameter PS particles, similar in size to the bacterial cells, inhibited the growth of both E. coli and Bacillus sp. cells the most. The E. coli and Bacillus sp. cells formed microPS-biofilm complex by secreting an extracellular polymeric substance (EPS) in response to their exposure to the ∼ 1-μm-diameter PS particles. A positive correlation between relative ROS levels and specific growth rates of the E. coli cells were observed with a Pearson correlation coefficient r value of 0.676 (p < 0.05).
Topics: Bacteria; Escherichia coli; Extracellular Polymeric Substance Matrix; Microscopy; Plastics; Polystyrenes; Reactive Oxygen Species; Water Pollutants, Chemical
PubMed: 35798153
DOI: 10.1016/j.chemosphere.2022.135584 -
International Journal of Biological... May 2023Nanoplastics are an emerging environmental contaminant that can penetrate biological barriers to enter the bloodstream and risk human health. In this context,...
Nanoplastics are an emerging environmental contaminant that can penetrate biological barriers to enter the bloodstream and risk human health. In this context, nanoplastics are likely to interact with proteins in the blood to possibly affect protein structure and function and consequently induce biological effects. Here we report that polystyrene (PS), PS-NH, and PS-COOH nanoplastics disrupt the structure of human fibrinogen (HF) in a dose-dependent manner, as revealed by UV-vis and fluorescence spectroscopy. All three nanoplastics interacted with HF in a similar way, with PS-NH having the greatest effect on HF structure. Furthermore, fibrinogen polymerization experiments demonstrated that nanoplastics have the potential to promote blood coagulation, with PS-NH again having a stronger effect. Collectively, these results provide insights into the interactions occurring between nanoplastics and HF, the likely transport and fate of nanoplastics in organisms, and their potential pathophysiological consequences.
Topics: Humans; Polystyrenes; Microplastics; Fibrinogen; Nanoparticles; Water Pollutants, Chemical
PubMed: 36931485
DOI: 10.1016/j.ijbiomac.2023.124049 -
International Journal of Molecular... Nov 2023Polymers' controlled pyrolysis is an economical and environmentally friendly solution to prepare activated carbon. However, due to the experimental difficulty in...
Polymers' controlled pyrolysis is an economical and environmentally friendly solution to prepare activated carbon. However, due to the experimental difficulty in measuring the dependence between microstructure and pyrolysis parameters at high temperatures, the unknown pyrolysis mechanism hinders access to the target products with desirable morphologies and performances. In this study, we investigate the pyrolysis process of polystyrene (PS) under different heating rates and temperatures employing reactive molecular dynamics (ReaxFF-MD) simulations. A clear profile of the generation of pyrolysis products determined by the temperature and heating rate is constructed. It is found that the heating rate affects the type and amount of pyrolysis intermediates and their timing, and that low-rate heating helps yield more diverse pyrolysis intermediates. While the temperature affects the pyrolytic structure of the final equilibrium products, either too low or too high a target temperature is detrimental to generating large areas of the graphitized structure. The reduced time plots (RTPs) with simulation results predict a PS pyrolytic activation energy of 159.74 kJ/mol. The established theoretical evolution process matches experiments well, thus, contributing to preparing target activated carbons by referring to the regulatory mechanism of pyrolytic microstructure.
Topics: Polystyrenes; Molecular Dynamics Simulation; Pyrolysis; Temperature; Heating
PubMed: 38003591
DOI: 10.3390/ijms242216403