-
Environmental Pollution (Barking, Essex... Feb 2023Nanoplastics (NPs) pollution has become an emerging threat to the aquatic environment and its organisms. The removal of NPs from contaminated water is a global...
Nanoplastics (NPs) pollution has become an emerging threat to the aquatic environment and its organisms. The removal of NPs from contaminated water is a global challenge. In this study, an efficient and reusable composite was prepared from cetyltrimethylammonium bromide (CTAB) modified magnetic biochar. The performances of CTAB modified magnetic biochar (CMB) to remove polystyrene (PS) and carboxylate-modified polystyrene (CPS) nanoparticles from water were systematically evaluated. The results showed that the PS and CPS removal performance of magnetic biochar was improved by CTAB modification. These increases were assigned to the increase in the surface hydrophobicity of CMB. Due to the strong electrostatic repulsion between the nanoparticles, PS and CPS maintained high stability in alkaline conditions, resulting in a significant decrease in removal efficiency. The removal efficiency was decreased to 67.4% for PS and to 40.7% for CPS at pH 11. The inhibition effects of NaCl on the PS and CPS removal efficiencies were decreased gradually with the increase of NaCl concentration. Among the anions studied, HPO had the biggest impact on the removal performance of CMB. Besides, CMB could be used to remove PS and CPS in real surface water, and the removal efficiencies of PS and CPS were 95.3% and 97.8%, respectively. Particularly, the removal efficiencies of PS and CPS were 90.2% for PS and 94.8% for CPS when CMB was recycled five times. According to the characterization results of XRD, TGA, SEM, FTIR and XPS, PS and CPS nanoparticles were removed by CMB from water mainly through aggregation instead of adsorption. The efficient removal of PS and CPS by CMB via aggregation process offers new insight into the removal of NPs from aquatic environment.
Topics: Cetrimonium; Polystyrenes; Microplastics; Sodium Chloride; Adsorption; Carboxylic Acids; Magnetic Phenomena; Water Pollutants, Chemical
PubMed: 36539007
DOI: 10.1016/j.envpol.2022.120897 -
ACS Macro Letters Apr 2022The interfacial strength of polystyrene (PS) with and without PS oligomers in contact with a glass substrate was examined to determine the relationship between the...
The interfacial strength of polystyrene (PS) with and without PS oligomers in contact with a glass substrate was examined to determine the relationship between the interfacial aggregation state and adhesion. The shear bond strength and adsorbed layer thickness of neat PS exhibited a similar dependence on the thermal annealing time: they increased to constant values within almost the same time. This implies that the adhesion of the polymer is closely related to the formation of an adsorbed layer at the adhesion interface. Nevertheless, in the case of PS with a small amount of oligomer, the shear bond strength decreased, while the adsorbed layer thickness was almost the same as that of neat PS. Based on the results of interfacial analyses, we propose that the interfacial segregation of the oligomer reduced the entanglement between the interfacial free chains in the adsorbed layer and the bulk chains.
Topics: Polymers; Polystyrenes; Shear Strength; Surface Properties
PubMed: 35575338
DOI: 10.1021/acsmacrolett.2c00062 -
Environmental Pollution (Barking, Essex... Apr 2018Microplastic (MP) are environmental pollutants and have the potential to cause varying degrees of aquatic toxicity. In this study, the effects on gut microbiota of adult...
Microplastic (MP) are environmental pollutants and have the potential to cause varying degrees of aquatic toxicity. In this study, the effects on gut microbiota of adult male zebrafish exposed for 14 days to 100 and 1000 μg/L of two sizes of polystyrene MP were evaluated. Both 0.5 and 50 μm-diameter spherical polystyrene MP increased the volume of mucus in the gut at a concentration of 1000 μg/L (about 1.456 × 10 particles/L for 0.5 μm and 1.456 × 10 particles/L for 50 μm). At the phylum level, the abundance of Bacteroidetes and Proteobacteria decreased significantly and the abundance of Firmicutes increased significantly in the gut after 14-day exposure to 1000 μg/L of both sizes of polystyrene MP. In addition, high throughput sequencing of the 16S rRNA gene V3-V4 region revealed a significant change in the richness and diversity of microbiota in the gut of polystyrene MP-exposed zebrafish. A more in depth analysis, at the genus level, revealed that a total of 29 gut microbes identified by operational taxonomic unit (OTU) analysis were significantly changed in both 0.5 and 50 μm-diameter polystyrene MP-treated groups. Moreover, it was observed that 0.5 μm polystyrene MP not only increased mRNA levels of IL1α, IL1β and IFN but also their protein levels in the gut, indicating that inflammation occurred after polystyrene MP exposure. Our findings suggest that polystyrene MP could induce microbiota dysbiosis and inflammation in the gut of adult zebrafish.
Topics: Animals; Bacteroidetes; Dysbiosis; Fish Diseases; Gastroenteritis; Male; Particle Size; Polystyrenes; Proteobacteria; RNA, Ribosomal, 16S; Zebrafish
PubMed: 29304465
DOI: 10.1016/j.envpol.2017.12.088 -
The Science of the Total Environment May 2022The knowledge about the interaction of nanoplastics with other aquatic pollutants and their combined effects on biota is very scarce. In this work, we studied the...
The knowledge about the interaction of nanoplastics with other aquatic pollutants and their combined effects on biota is very scarce. In this work, we studied the interaction between polystyrene nanoplastics (PS NPs) (30 nm) and the micropollutants in a biologically treated wastewater effluent (WW). The capacity of PS NPs to sorb micropollutants was studied as well as their single and combined toxicity towards three freshwater organisms: the recombinant bioluminescent cyanobacterium, Anabaena sp. PCC 7120 CPB4337; the duckweed, Spirodela polyrhiza and the cladoceran, Daphnia magna. The endpoints were the inhibition of bioluminescence, the growth inhibition of the aquatic plant and the immobilization of D. magna after 24, 72 and 48 h of exposure, respectively. Combination Index (CI)-isobologram method was used to quantify mixture toxicity and the nature of interactions. PS NPs sorbed a variety of chemicals present in WW as micropollutants in a range of tens of ng/L to μg/L. It was found that those pollutants with positive charge were the main ones retained onto PS NPs, which was attributed to the electrostatic interaction with the negatively charged PS NPs. Regarding the toxicological effects, single exposure to PS NPs affected the three tested organisms. However, single exposure to WW only had a negative impact on the cyanobacterium and S. polyrhiza with no observed toxicity to D. magna. Regarding PS NPs-WW combined exposure, a reduction of toxicity in comparison with single exposure was observed probably due to the sorption of micropollutants onto PS NPs, which resulted in lower bioavailability of the micropollutants. In addition, the formation of PS NPs-WW heteroaggregates was observed which could result in lower bioavailability of PS NPs and sorbed micropollutants, thus lowering toxicity. This study represents a near-realistic scenario approach to the potential sorption of wastewater pollutants onto nanoplastics that could alter the toxicological effect on the biota.
Topics: Microplastics; Nanoparticles; Polystyrenes; Wastewater; Water Pollutants, Chemical
PubMed: 35031361
DOI: 10.1016/j.scitotenv.2022.153063 -
Chemosphere Oct 2023Pollution by microplastics in aquatic ecosystems is a worldwide problem, and the role of microplastics as vectors of pollutants has been a concern. Although small...
Pollution by microplastics in aquatic ecosystems is a worldwide problem, and the role of microplastics as vectors of pollutants has been a concern. Although small microplastics are thought to have a greater effect than large microplastics as vectors of pollutants, the impact of the size of microplastics on their ability to serve as vectors of pollutants has not been quantified. In this study, we conducted the 14-day experiment (7 days of exposure and 7 days of depuration) with polystyrene microplastics (2-μm or 10-μm diameter) and anthracene. On the last day of the exposure period, the concentration of anthracene in the muscle of Java medaka exposed to both anthracene and 2-μm polystyrene microplastics was the highest (47.4 ± 15.2 μg/g-muscle) of any group, followed by the group exposed to both anthracene and 10-μm polystyrene microplastics (23.0 ± 4.2 μg/g-muscle) and the group exposed to only anthracene (11.2 ± 2.2 μg/g-muscle). These results demonstrated that the size of microplastics was a critical determinant of their ability to serve as vectors of anthracene. The concentrations of anthracene and fine microplastics in the environment are sufficiently low that the effect of microplastics as vectors of anthracene may be observed only under experimental conditions that are unlikely to occur in the present environment. However, because pollution by plastics is expected to become more serious in the future, careful thought and proactive action will be needed to ensure that the impact of microplastics as vectors of pollutants does not become demonstrable under future environmental conditions.
Topics: Animals; Microplastics; Polystyrenes; Plastics; Oryzias; Ecosystem; Water Pollutants, Chemical; Environmental Pollutants; Anthracenes
PubMed: 37474033
DOI: 10.1016/j.chemosphere.2023.139543 -
Macromolecular Bioscience Aug 2023Polystyrene is one of the most widely used plastics. This article reports on the interaction of 50 and 210 nm polystyrene nanoparticles (PSNPs) with human serum...
Polystyrene is one of the most widely used plastics. This article reports on the interaction of 50 and 210 nm polystyrene nanoparticles (PSNPs) with human serum albumin (HSA) and transferrin (Tf), as well as their effect on supported lipid bilayers (SLBs), using experimental and theoretical approaches. Dynamic light scattering (DLS) and atomic force microscopy (AFM) measurements show that the increase in diameter for the PSNP-protein bioconjugates depends on nanoparticle size and type of proteins. The circular dichroism (CD) spectroscopy results demonstrate that the proteins preserve their structures when they interact with PSNPs at physiological temperatures. The quartz crystal microbalance (QCM) technique reveals that PSNPs and their bioconjugates show no strong interactions with SLBs. On the contrary, the molecular dynamics simulations (MDS) show that both proteins bind strongly to the lipid bilayer (SLBs) when compared to their binding to a polystyrene surface model. The interaction is strongly dependent on the protein and lipid bilayer composition. Both the PSNPs and their bioconjugates show no toxicity in human umbilical vein endothelial (HUVEC) cells; however, bare 210 nm PSNPs and 50 nm PSNP-Tf bioconjugates show an increase in reactive oxygen species production. This study may be relevant for assessing the impact of plastics on health.
Topics: Humans; Lipid Bilayers; Polystyrenes; Protein Corona; Nanoparticles; Plastics
PubMed: 36707930
DOI: 10.1002/mabi.202200464 -
The Science of the Total Environment Oct 2022Microplastics have become pervasive environmental pollutants, especially in freshwater rivers and lakes. However, how freshwater prawns' reproductive system is affected...
Microplastics have become pervasive environmental pollutants, especially in freshwater rivers and lakes. However, how freshwater prawns' reproductive system is affected by polystyrene microplastics (PS-MPs) remains incompletely understood. Thus, the present study aimed to determine the effect of PS-MPs on the male reproductive system and offspring larval immunity in oriental river prawn. Acute exposure to PS-MPs decreased the survival rate and heart rate of prawn larvae. After chronic exposure to PS-MPs (2 and 20 mg/L) for four weeks, the oxidative stress generation in testis tissue indicated a negative impact on male prawn testicular function. PS-MPs disrupted testicular germ cell quality and caused sex hormone imbalance, leading to reduced hatching success and survival of F1 larvae, despite not being exposed to PS-MPs. Steroidogenic gene expression was altered and apoptosis-related genes had higher expression in the gonads after parental exposure to PS-MPs. Decreased immunity-related enzyme activities were observed in F1 larvae with/without continued PS-MPs exposure, compared with those in untreated prawns. A concentration-dependent increase in bioaccumulation of PS-MPs in different tissues of larval offspring was observed. Thus, PS-MPs had multiple effects on male reproductive dysfunction and transgenerational toxicity in prawns. Our findings provide a novel insight into the reproductive toxicity mechanism of microplastics in freshwater crustaceans.
Topics: Animals; Fresh Water; Larva; Male; Microplastics; Palaemonidae; Plastics; Polystyrenes; Water Pollutants, Chemical
PubMed: 35738382
DOI: 10.1016/j.scitotenv.2022.156820 -
Journal of Hazardous Materials Jul 2022As a ubiquitous emerging pollutant, microplastics (MPs) have attracted widespread attention. At this stage, researchers mainly employed commercial MPs (CMPs) as the...
As a ubiquitous emerging pollutant, microplastics (MPs) have attracted widespread attention. At this stage, researchers mainly employed commercial MPs (CMPs) as the model particles to explore the toxic effects of MPs. But whether CMPs can reflect the effects of realistic MPs (RMPs) still remains unknown. Herein, the effects of commercial and realistic polystyrene MPs on gut microbiota of zebrafish were compared. Considering MPs co-exist with antibiotics in real environment, we further distinguished the effects of CMPs and RMPs when they co-existed with enrofloxacin (ENR). The results revealed that while both CMPs and RMPs significantly shifted the gut microbiota, CMPs exhibited stronger toxic effects and more severe damage to gut. Furthermore, ENR exhibited a distinct effect with both CMPs and RMPs on gut microbiota, while the addition of CMPs and RMPs significantly alleviated the toxicity of ENR. In addition, analysis via Kyoto Encyclopedia of Genes and Genomes pathway database revealed that seven major level 1 pathways associated with metabolism, information processing and diseases in the microbial community were affected. Taken together, this work is the first to report that CMPs could not represent RMPs in terms of toxicity and other behaviors, reminding people the limits of using CMPs in ecotoxicology studies.
Topics: Animals; Humans; Microbiota; Microplastics; Plastics; Polystyrenes; Water Pollutants, Chemical; Zebrafish
PubMed: 35429760
DOI: 10.1016/j.jhazmat.2022.128874 -
Polystyrene nanoplastics enhance the toxicological effects of DDE in zebrafish (Danio rerio) larvae.The Science of the Total Environment Feb 2023Anthropogenic releases of plastics, persistent organic pollutants (POPs), and heavy metals can impact the environment, including aquatic ecosystems. Nanoplastics (NPs)...
Anthropogenic releases of plastics, persistent organic pollutants (POPs), and heavy metals can impact the environment, including aquatic ecosystems. Nanoplastics (NPs) have recently emerged as pervasive environmental pollutants that have the ability to adsorb POPs and can cause stress in organisms. Among POPs, DDT and its metabolites are ubiquitous environmental pollutants due to their long persistence. Despite the discontinued use of DDT in Europe, DDT and its metabolites (primarily p,p'-DDE) are still found at detectable levels in fish feed used in salmon aquaculture. Our study aimed to look at the individual and combined toxicity of NPs (50 mg/L polystyrene) and DDE (100 μg/L) using zebrafish larvae as a model. We found no significant morphological, cardiac, respiratory, or behavioural changes in zebrafish larvae exposed to NPs alone. Conversely, morphological, cardiac and respiratory alterations were observed in zebrafish larvae exposed to DDE and NPs + DDE. Interestingly, behavioural changes were only observed in zebrafish larvae exposed to NPs + DDE. These findings were supported by RNA-seq results, which showed that some cardiac, vascular, and immunogenic pathways were downregulated only in zebrafish larvae exposed to NPs + DDE. In summary, we found an enhanced toxicological impact of DDE when combined with NPs.
Topics: Animals; Zebrafish; Larva; Polystyrenes; Ecosystem; Water Pollutants, Chemical; Environmental Pollutants
PubMed: 36435242
DOI: 10.1016/j.scitotenv.2022.160457 -
Marine Pollution Bulletin Feb 2023Jellyfish are planktonic predators that may be susceptible to ingesting microplastics. However, the effects of MP exposure on jellyfish are poorly understood. In this...
Jellyfish are planktonic predators that may be susceptible to ingesting microplastics. However, the effects of MP exposure on jellyfish are poorly understood. In this study, the ingestion and egestion of polystyrene microbeads, and its chronic physiological effects on Rhopilema esculentum at an environmental concentration (100 items/L) and a predicted concentration (1000 items/L) were evaluated. The results showed that the ingestion amount of juvenile medusae was relatively low. The MP egestion rates reached 100 % within 9 h of clearance. Chronic exposure (15 days) to MPs at environmental concentrations led to no adverse impacts. Nevertheless, the predicted concentration of MP exposure induced growth inhibition, a reduction in assimilation efficiency, oxygen consumption increase, and lipase enzyme activity reduction in the jellyfish, indicating that MPs can cause adverse effects on the energy budget of jellyfish in the near future. Our study provides new insights into the potential risk of MPs in marine environments.
Topics: Animals; Microplastics; Polystyrenes; Plastics; Water Pollutants, Chemical; Scyphozoa; Eating
PubMed: 36652861
DOI: 10.1016/j.marpolbul.2023.114609