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Journal of Hazardous Materials Feb 2022Uranium is a heavy metal with both chemotoxicity and radiotoxicity. Due to the increasing consumption of uranium, the remediation of uranium contamination and recovery...
Uranium is a heavy metal with both chemotoxicity and radiotoxicity. Due to the increasing consumption of uranium, the remediation of uranium contamination and recovery of uranium from non-conventional approach is highly needed. Microorganism exhibits high potential for immobilization of uranium. This study for the first time isolated a marine Pseudomonas stutzeri strain MRU-UE1 with high uranium immobilization capacity of 308.72 mg/g, which is attributed to the synergetic mechanisms of biosorption, biomineralization, and bioreduction. The uranium is found to be immobilized in forms of tetragonal chernikovite (H(UO)(PO)·8HO) by biomineralization and CaU(PO) by bioreduction under aerobic environment, which is rarely observed and would broaden the application of this strain in aerobic condition. The protein, phosphate group, and carboxyl group are found to be essential for the biosorption of uranium. In response to the stress of uranium, the strain produces inorganic phosphate group, which transformed soluble uranyl ion to insoluble uranium-containing precipitates, and poly-β-hydroxybutyrate (PHB), which is observed for the first time during the interaction between microorganism and uranium. In summary, P. stutzeri strain MRU-UE1 would be a promising alternative for environmental uranium contamination remediation and uranium extraction from seawater.
Topics: Biodegradation, Environmental; Biomineralization; Phosphates; Pseudomonas stutzeri; Uranium
PubMed: 34801303
DOI: 10.1016/j.jhazmat.2021.127758 -
Marine Biotechnology (New York, N.Y.) Feb 2023A marine aerobic denitrifying bacterium was isolated and identified as Pseudomonas stutzeri BBW831 from the seabed silt of Beibu Gulf in China. According to the genome...
Nitrogen Removal Characteristics of a Marine Denitrifying Pseudomonas stutzeri BBW831 and a Simplified Strategy for Improving the Denitrification Performance Under Stressful Conditions.
A marine aerobic denitrifying bacterium was isolated and identified as Pseudomonas stutzeri BBW831 from the seabed silt of Beibu Gulf in China. According to the genome analysis, P. stutzeri BBW831 possessed a total of 14 genes (narG, narH, narI, narJ, napA, napB, nirB, nirD, nirS, norB, norC, norD, norQ, and nosZ) responsible for fully functional enzymes (nitrate reductase, nitrite reductase, nitric oxide reductase, and nitrous oxide reductase) involved in the complete aerobic denitrification pathway, suggesting that it had the potential for reducing nitrate to the final N. Denitrification results showed that P. stutzeri BBW831 exhibited efficient nitrogen removal characteristics. Within 12 h, the NO-N removal efficiency and rate reached 94.64% and 13.09 mg·L·h under 166.10 ± 3.75 mg/L NO-N as the sole nitrogen source, and removal efficiency of the mixed nitrogen (50.50 ± 0.55, 62.28 ± 0.74, and 64.26 ± 0.90 mg/L of initial NH-N, NO-N, and NO-N, respectively) was nearly 100%. Furthermore, a simplified strategy, by augmenting the inoculation biomass, was developed for promoting the nitrogen removal performance under high levels of NO-N and salinity. As a result, the removal efficiency of the initial NO-N up to approximately 130 mg/L reached 99.46% within 8 h, and the NO-N removal efficiency achieved at 59.46% under the NaCl concentration even up to 50 g/L. The C/N ratio of 10 with organic acid salt such as trisodium citrate and sodium acetate as the carbon source was most conducive for cell growth and nitrogen removal by P. stutzeri BBW831, respectively. In conclusion, the marine P. stutzeri BBW831 contained the functional genes responsible for a complete aerobic denitrification pathway (NO-N → NO-N → NO → NO → N), and had great potential for the practical treatment of high-salinity nitrogenous mariculture wastewater.
Topics: Denitrification; Nitrates; Nitrogen; Nitrogen Dioxide; Pseudomonas stutzeri
PubMed: 36446961
DOI: 10.1007/s10126-022-10185-1 -
3 Biotech Feb 2023Toxic polycyclic aromatic hydrocarbons (PAHs) are often released into the environment during the combustion and processing of fossil fuels and are capable of causing...
UNLABELLED
Toxic polycyclic aromatic hydrocarbons (PAHs) are often released into the environment during the combustion and processing of fossil fuels and are capable of causing significant pollution to people and the environment. One of the representative substances of PAHs is phenanthrene, which is often studied as a model compound for PAHs treatment. In this study, we compared the results of transcriptome analysis of in two different culture conditions under phenanthrene-induced culture (test group) and glucose-induced culture (control group), and analysed the key enzymatic mechanisms of in the biodegradation of phenanthrene. In our experiments, the transcriptome results showed that a total of 380 genes were more than twofold differentially expressed in the test group, of which 187 genes were significantly up-regulated in expression under Phenanthrene induction. Among the 380 differentially expressed genes, 90 genes were involved in Phenanthrene biodegradation, mainly including genes involved in biometabolism, cellular chemotaxis, substrate transport, signal induction and other related processes. Based on the transcriptome sequence analysis of at the time of phenanthrene induction, a total of 25 dioxygenase genes were identified, and the related genes were mainly concentrated in two relatively concentrated clusters of PAHs biodegradation genes. The transcriptome analysis resulted in a complete set of enzyme genes related to the phenanthrene biodegradation pathway. The analysis of key enzymes led to the inference of a possible phenanthrene biodegradation pathway: the salicylic acid degradation pathway. The results of this study provide a theoretical basis for in situ remediation of PAHs-contaminated environments using .
SUPPLEMENTARY INFORMATION
The online version contains supplementary material available at 10.1007/s13205-023-03473-7.
PubMed: 36718409
DOI: 10.1007/s13205-023-03473-7 -
3 Biotech Oct 2017The present study focuses on the optimization of biosurfactant (BS) production using two potential biosurfactant producer NA3 and MN3 and role of enzymes in the...
The present study focuses on the optimization of biosurfactant (BS) production using two potential biosurfactant producer NA3 and MN3 and role of enzymes in the biodegradation of crude oil. The optimal conditions for NA3 and MN3 for biodegradation were pH of 8 and 7; temperature of 30 and 40 °C, respectively. NA3 and MN3 produced 3.81 and 4.68 g/L of BS, respectively. Gas chromatography mass spectrometry confirmed that BS was mainly composed of fatty acids. Furthermore, the role of the degradative enzymes, alkane hydroxylase, alcohol dehydrogenase and laccase on biodegradation of crude oil are explained. Maximum biodegradation efficiency (BE) was recorded for mixed consortia (86%) followed by strain NA3 (84%). Both bacterial strains were found to be vigorous biodegraders of crude oil than other biosurfactant-producing bacteria due to their enzyme production capabilities and our results suggests that the bacterial isolates can be used for effective degradation of crude oil within short time periods.
PubMed: 28794933
DOI: 10.1007/s13205-017-0902-7 -
Iranian Journal of Microbiology Apr 2020Excess use of pesticides in agricultural field not only compromised soil fertility but also posed serious threat to water bodies and life in the surrounding environment....
BACKGROUND AND OBJECTIVES
Excess use of pesticides in agricultural field not only compromised soil fertility but also posed serious threat to water bodies and life in the surrounding environment. The leftover pesticide residue needs to be remediated effectively. Compared to physical, chemical and enzymatic remediation options the microbial remediation is more practical and sustainable.
MATERIALS AND METHODS
The smk strain was found to use dichlorvos as the solitary carbon source. Minimal medium supplemented with dichlorvos was used to test ability of bacterium to degrade pesticide aerobically. The metabolites produced by the bacterium were studied with UV-Vis spectrophotometry, HPLC, FTIR and GC-MS techniques. The toxicity studies of neat dichlorvos and smk degraded metabolites were studied by subcutaneous injection in .
RESULTS
The smk strain was found to degrade as high as 80% of dichlorvos on 7 day of incubation, at 30 °C temperature and at pH 7. In five steps complete aerobic degradation of 2,2dicholorvinyl dimethyl phosphate (dichlorvos) resulted in production of free methyl and phosphate. The degradation intermediates produced are 2-Chlorovinyl dimethyl phosphate, vinyl dimethyl phosphate, dimethyl phosphate, methylphosphate and finally free phosphate. The histopathological analysis of liver, spleen and thymus of s were performed to study toxicity of dichlorvos and degraded metabolites.
CONCLUSION
smk could result highest aerobic degradation of dichlorvos to produce free methyl and phosphate. Degradation metabolites could reverse largely toxic effects of dichlorvos when studied in .
PubMed: 32494348
DOI: No ID Found -
Scientific Reports Feb 2021The bacterium Pseudomonas stutzeri SPM-1, obtained from textile wastewater dumping sites of Surat, Gujarat was studied for the degradation of the textile azo dye Procion...
The bacterium Pseudomonas stutzeri SPM-1, obtained from textile wastewater dumping sites of Surat, Gujarat was studied for the degradation of the textile azo dye Procion Red-H3B. The optimization was carried on the phenanthrene enrichment medium followed by exposing it to variable environmental factors and nutritional sources. The complete decolorization of dye (50 mg/L) happened within 20 h of incubation at pH 8 and temperature 32 ± 0.2 °C under microaerophilic conditions. Decolourization was monitored with the shifting of absorbance peak in UV-Vis spectrophotometry and HPLC analysis. The physicochemical studies of effluent before and after the treatment revealed 85%, 90%, and 65% decline in BOD, COD, and TOC levels. The strain showed significant activities of azoreductase (95%), laccase (76%), and NADH-DCIP reductase (88%) at 12 h, 10 h, and 8 h of growth respectively indicating evidence for reductive cleavage of the dye. The changes in the functional groups were confirmed by the presence of new peaks in FT-IR data. GC-MS analysis helped in recognizing the degraded dye compounds thus elucidating the proposed pathway for degradation of Procion Red-H3B. The potential of the bioremediation process was concluded by a phytotoxicity test using two plants, Vigna radiata and Cicer arietinum. Our study demonstrates that the strain Pseudomonas stutzeri SPM-1 has rapid decolorization efficiency and holds a noteworthy perspective in industrial application for textile wastewater treatment.
Topics: Azo Compounds; Biodegradation, Environmental; Gas Chromatography-Mass Spectrometry; Humans; Pseudomonas stutzeri; Spectroscopy, Fourier Transform Infrared; Triazines; Wastewater; Water Pollutants, Chemical; Water Purification
PubMed: 33542307
DOI: 10.1038/s41598-021-82494-9 -
Bioscience, Biotechnology, and... Jul 2023d-Aldotetroses are rare sugars that are obtained via chemical synthesis in low yield. In this study, we demonstrated that d-aldotetroses could be produced using 3...
d-Aldotetroses are rare sugars that are obtained via chemical synthesis in low yield. In this study, we demonstrated that d-aldotetroses could be produced using 3 isomerases. First, l-erythrulose was epimerized using d-tagatose 3-epimerase from Pseudomonas cichorii ST-24. The specific optical rotation of the reaction solution gradually decreased to zero, indicating that approximately 50% of the l-erythrulose was converted to d-erythrulose. d, l-Erythrulose mixture was isomerized with d-arabinose isomerase from Klebsiella pneumoniae 40bXX to produce d-threose, resulting in a conversion rate of 9.35%. d-Erythrose production using l-rhamnose isomerase from Pseudomonas stutzeri LL172 resulted in a conversion rate of 12.9%. Because of the low purity of the purchased d-erythrose, the product was reduced by the Raney nickel catalyst compared with authentic erythritol. We confirmed the products using HPLC and 13C-NMR spectra. This is the first report of d-aldotetrose production using an enzymatic reaction.
Topics: Tetroses; Hexoses; Isomerases; Aldose-Ketose Isomerases; Racemases and Epimerases
PubMed: 37156528
DOI: 10.1093/bbb/zbad058 -
Journal of Visualized Experiments : JoVE Jan 2022Melanins are natural pigments, and the presence of indole ring and numerous functional groups makes melanin an ideal choice for many applications such as UV protective...
Melanins are natural pigments, and the presence of indole ring and numerous functional groups makes melanin an ideal choice for many applications such as UV protective agents, skincare, cosmetics etc. A marine Pseudomonas stutzeri produces melanin without the addition of tyrosine. The feedback inhibition was observed by melanin in the culture of a melanin-producing marine bacterium, Pseudomonas stutzeri. Melanin also demonstrated microbial growth inhibition. The Han-Levenspiel model-based analysis identified uncompetitive type product inhibition of melanin on the cell growth. Tyrosinase enzyme, which produces melanin, was inhibited by melanin. The double reciprocal plot of the enzymatic reaction in the presence of different melanin concentrations revealed uncompetitive product inhibition. An adsorbent-based adsorptive bioprocess was developed to reduce the feedback inhibition by melanin. Different adsorbents were screened to select the best adsorbent for melanin adsorption. Dosage amount and time were optimized to develop the adsorptive bioprocess, which resulted in an 8.8-fold enhancement in melanin production by the marine bacteria Pseudomonas stutzeri (153 mg/L to 1349 mg/L) without supplementation of tyrosine and yeast extract.
Topics: Adsorption; Cosmetics; Melanins; Pseudomonas stutzeri; Tyrosine
PubMed: 35098948
DOI: 10.3791/63339 -
Plant Physiology and Biochemistry : PPB Nov 2022Arsenic (As)-contaminated rice paddy fields are spreading globally, and thus, rice grains with low As accumulation at a safe level for consumption is profoundly needed....
Responses to arsenic stress of rice varieties coinoculated with the heavy metal-resistant and rice growth-promoting bacteria Pseudomonas stutzeri and Cupriavidus taiwanensis.
Arsenic (As)-contaminated rice paddy fields are spreading globally, and thus, rice grains with low As accumulation at a safe level for consumption is profoundly needed. Rice is highly susceptible to As accumulation, and the responses to As vary among rice varieties. Here, combinations of the As-oxidizing bacteria Pseudomonas stutzeri strains 4.25, 4.27, or 4.44 and Cupriavidus taiwanensis KKU2500-3 were investigated with respect to their responses to As toxicity and rice growth promotion during the early growth stage. All bacterial strains enhanced antioxidant enzyme activities, including SOD, CAT, APX, GPX, and GR, under As stress in vitro. Uninoculated and coinoculated rice seedlings of three rice varieties (KDML105, RD6, RD10) were cultivated in hydroponic solution without and with a combination of toxic As and less toxic As for 30 days. Compared with uninoculated seedlings, the inoculated seedlings showed higher growth parameters and lower As contents in roots, shoots and throughout the plants. The bioconcentration factor (BCF) and translocation factor were reduced in inoculated seedlings. The effective response of rice to As toxicity influenced by bacteria was highest in KDML105, followed by RD6 and RD10. The root sulfide content was correlated with As accumulation in roots, shoots, and total seedlings and the BCFs. P. stutzeri 4.44 and C. taiwanensis KKU2500-3 were the most promising combinations for application in KDML105 cultivation under As-contaminated conditions. Understanding the basic response of rice coinoculated with effective bacteria at the early stage will provide guidelines for rice cultivation under As conditions at other scales.
Topics: Antioxidants; Arsenic; Cupriavidus; Metals, Heavy; Oryza; Plant Roots; Pseudomonas stutzeri; Seedlings; Soil Pollutants; Sulfides; Superoxide Dismutase
PubMed: 36182828
DOI: 10.1016/j.plaphy.2022.09.014 -
Applied and Environmental Microbiology Feb 2021Diazotrophs can produce bioavailable nitrogen from inert N gas by bioelectrochemical nitrogen fixation (-BNF), which is emerging as an energy-saving and highly selective...
Diazotrophs can produce bioavailable nitrogen from inert N gas by bioelectrochemical nitrogen fixation (-BNF), which is emerging as an energy-saving and highly selective strategy for agriculture and industry. However, current -BNF technology is impeded by requirements for NH assimilation inhibitors to facilitate intracellular ammonia secretion and precious metal catalysts to generate H as the energy-carrying intermediate. Here, we initially demonstrate inhibitor- and catalystless extracellular NH production by the diazotroph Pseudomonas stutzeri A1501 using an electrode as the sole electron donor. Multiple lines of evidence revealed that P. stutzeri produced 2.32 ± 0.25 mg/liter extracellular NH at a poised potential of -0.3 V (versus standard hydrogen electrode [SHE]) without the addition of inhibitors or expensive catalysts. The electron uptake mechanism was attributed to the endogenous electron shuttle phenazine-1-carboxylic acid, which was excreted by P. stutzeri and mediated electron transfer from electrodes into cells to directly drive N fixation. The faradaic efficiency was 20% ± 3%, which was 2 to 4 times that of previous -BNF attempts using the H-mediated pathway. This study reports a diazotroph capable of producing secretable NH via extracellular electron uptake, which has important implications for optimizing the performance of -BNF systems and exploring the novel nitrogen-fixing mode of syntrophic microbial communities in the natural environment. Ammonia greatly affects global ecology, agriculture, and the food industry. Diazotrophs with an enhanced capacity of extracellular NH excretion have been proven to be more beneficial to the growth of microalgae and plants, whereas most previously reported diazotrophs produce intracellular organic nitrogen in the absence of chemical suppression and genetic manipulation. Here, we demonstrate that Pseudomonas stutzeri A1501 is capable of extracellular NH production without chemical suppression or genetic manipulation when the extracellular electrode is used as the sole electron donor. We also reveal the electron uptake pathway from the extracellular electron-donating partner to P. stutzeri A1501 via redox electron shuttle phenazines. Since both P. stutzeri A1501 and potential electron-donating partners (such as electroactive microbes and natural semiconductor minerals) are abundant in diverse soils and sediments, P. stutzeri A1501 has broader implications on the improvement of nitrogen fertilization in the natural environment.
Topics: Ammonia; Nitrogen Fixation; Pseudomonas stutzeri
PubMed: 33310714
DOI: 10.1128/AEM.01998-20