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Frontiers in Cellular and Infection... 2021Accumulative evidence suggests that gut microbiota play an important role in bone remodeling and hence bone health maintenance. This study aimed to explore the...
OBJECTIVE
Accumulative evidence suggests that gut microbiota play an important role in bone remodeling and hence bone health maintenance. This study aimed to explore the association of gut microbiota with the risk of osteoporosis and to identify potential disease-related taxa, which may be promising targets in osteoporosis prevention and treatment in the future.
METHODS
Absolute quantification 16S ribosomal RNA gene sequencing was used to detect absolute and relative abundances of gut microbiota in 44 patients with osteoporosis and 64 controls. In combination with one of our previous studies, a total of 175 samples were involved in the relative abundance analysis.
RESULTS
Compared with the controls, the patients with osteoporosis had higher absolute and relative abundances of Bacteroidetes phylum, and and genera. The absolute abundances of , , , and genera increased, and that of the genus decreased in the osteoporosis group. As for relative abundance, that of the and genera increased, whereas that of the genus decreased in the osteoporosis group. Controlling for potential confounders, the associations of , , and genera with the risk of osteoporosis did not maintain significance. Ridge regression analysis suggested that is associated with reduced bone mineral density (BMD) and T-score at lumbar spines, and is associated with increased BMD at the femoral neck. Functional predictions revealed that 10 Kyoto Encyclopedia of Genes and Genomes pathways were enriched in the osteoporosis group.
CONCLUSIONS
Gut microbiota compositions may contribute to the risk of osteoporosis. Several specific taxa and functional pathways are identified to associate with reduced bone density, thus providing epidemiologic evidence for the potential role of aberrant gut microbiota in osteoporosis pathogenesis.
Topics: Aged; China; Clostridiales; Feces; Gastrointestinal Microbiome; High-Throughput Nucleotide Sequencing; Humans; Osteoporosis; RNA, Ribosomal, 16S
PubMed: 33996619
DOI: 10.3389/fcimb.2021.630372 -
Applied Biochemistry and Biotechnology Dec 2022A high-performance immobilized bacterial strain coated with magnetic iron oxide nanoparticles was used for Basic Blue 41 azo dye (BB 41 dye) decolorization. To create...
A high-performance immobilized bacterial strain coated with magnetic iron oxide nanoparticles was used for Basic Blue 41 azo dye (BB 41 dye) decolorization. To create the coated bacterial strain, Raoultella Ornithinolytica sp. was isolated and identified under the accession number KT213695, then coated with manufactured magnetic iron oxide nanoparticles. SEM and SEM-EDX were used to characterize the coated bacteria and validate its morphological structure formation. The coated Raoultella Ornithinolytica sp. A1 (coated A1) generated a 95.20% decolorization for BB 41 dye at 1600 ppm starting concentration with an optimal dose of coated A1 5 mL/L, pH 8, under static conditions for 24 h at 37 °C. Continuous batch cycles were used, with BB 41 dye (1600 ppm) added every 24 h four times, to achieve a high decolorization efficiency of 80.14%. Furthermore, the metabolites of BB 41 dye biodegradation were investigated by gas chromatographic-mass spectrum analysis (GC-MS) and showed a less toxic effect on the bioindicator Artemia salina. Additionally, 5 mL/L of coated A1 demonstrated the highest decolorization rate (47.2%) when applied to a real wastewater sample after 96 h with a consequent reduction in COD from 592 to 494 ppm.
Topics: Azo Compounds; Coloring Agents; Biodegradation, Environmental; Bacteria; Nanoparticles; Magnetic Phenomena
PubMed: 35881226
DOI: 10.1007/s12010-022-04076-3 -
Microorganisms Mar 2022Although (nasturtium) is an agriculturally and economically important plant, especially due to the presence of edible flowers and its medicinal properties, its...
Although (nasturtium) is an agriculturally and economically important plant, especially due to the presence of edible flowers and its medicinal properties, its microbiome is quite unexplored. Here, the structure of the total bacterial community associated with the rhizosphere, endosphere and bulk soil of was determined by 16S rRNA amplicon metagenomic sequencing. A decrease in diversity and richness from bulk soil to the rhizosphere and from the rhizosphere to the endosphere was observed in the alpha diversity analyses. The phylum Proteobacteria was the most dominant in the bacteriome of the three sites evaluated, whereas the genera and showed a significantly higher relative abundance in the rhizosphere and endosphere communities, respectively. Plant growth-promoting bacteria (236 PGPB) were also isolated from the endosphere, and 76 strains belonging to 11 different genera, mostly , and , showed positive results for at least four out of six plant growth-promoting tests performed. The selection of PGPB associated with can result in the development of a biofertilizer with activity against phytopathogens and capable of favoring the development of this important plant.
PubMed: 35336212
DOI: 10.3390/microorganisms10030638 -
Environmental Monitoring and Assessment Mar 2022The alarming upsurge in the co-existence of heavy metal and antibiotic resistance may have a devastating impact on humans, animals, and the environment. Four...
The alarming upsurge in the co-existence of heavy metal and antibiotic resistance may have a devastating impact on humans, animals, and the environment. Four metal-resistant bacteria were isolated from hospital effluents and industrial drain. Heavy metal resistance and antimicrobial resistance were examined in the isolates followed by identification through 16S rRNA gene sequencing. Delftia tsuruhatensis strain FK-01 and Carnobacterium inhibens strain FK-02 tolerated arsenic with maximal tolerated concentration (MTC) of 30 mM and 10 mM, respectively. Staphylococcus hominis strain FK-04 tolerated copper up to 4 mM and lead-resistant Raoultella ornithinolytica strain FK-05 exhibited tolerance to 1 mM lead. The growth kinetics of bacteria were monitored in the presence of metals and the following antibiotics, tetracycline, chloramphenicol, and kanamycin. The presence of arsenate significantly enhanced tetracycline resistance in C. inhibens. Heavy metal-induced antibiotic resistance was also observed in S. hominis and R. ornithinolytica, against chloramphenicol and tetracycline respectively. D. tsuruhatensis showed resistance to kanamycin but when grown in the presence of arsenic and kanamycin, bacteria lost resistance to the antibiotic. Therefore, it is suggested that the novel arsenate-resistant strain Delftia tsuruhatensis FK-01 has a unique ability to inhibit antimicrobial resistance that can be harnessed in bioremediation.
Topics: Anti-Bacterial Agents; Bacteria; Drug Resistance, Bacterial; Environmental Monitoring; Humans; Metals, Heavy; RNA, Ribosomal, 16S; Wastewater
PubMed: 35254524
DOI: 10.1007/s10661-022-09917-6 -
Medicina Clinica Jan 2022Raoultella spp. is a gram-negative bacillus with increasing clinical importance due to the development of multi-drug resistance and because it has been reported as a...
INTRODUCTION
Raoultella spp. is a gram-negative bacillus with increasing clinical importance due to the development of multi-drug resistance and because it has been reported as a cause of invasive infection. The risk of infection increases if comorbidities present such as diabetes mellitus and malignancies.
METHODS
Descriptive study of clinical and microbiological characteristics in adult patients with Raoultella spp. isolation, treated at a fourth-level hospital in Bogotá, Colombia, between 2015 and 2020.
RESULTS
61 patients with isolation of Raoultella spp., 51 were considered infection and 10, colonization. The associated comorbidities were hypertension (n=26, 42.6%), heart failure (n=19, 31.1%) and diabetes mellitus (n=18, 29.5%). AmpC resistance patterns were found in 10 samples (16.4%) and KPC in 3 (4.9%).
CONCLUSIONS
Raoultella spp. is of clinical importance due to its isolation in immunocompromised patients with multiple comorbidities and due to the increase in multi-resistant strains.
Topics: Adult; Anti-Bacterial Agents; Colombia; Enterobacteriaceae; Enterobacteriaceae Infections; Humans
PubMed: 33558064
DOI: 10.1016/j.medcli.2020.10.024 -
Microorganisms Nov 2021spp. is a relevant pathogen that can present acquired resistance to almost all available antibiotics, thus representing a serious threat for public health. While most...
spp. is a relevant pathogen that can present acquired resistance to almost all available antibiotics, thus representing a serious threat for public health. While most studies have been focused on isolates causing community-acquired and nosocomial infections, little is known about the commensal isolates colonizing healthy subjects. We describe the molecular identification and the phenotypic characterization of commensal spp. from breast milk of healthy women and faeces from healthy breast-fed infants, which were compared with isolates from community-acquired infections and from a nosocomial NICU outbreak. The phylogenetic analysis of a 454-bp sequence of the gene was useful for species identification (, , , , , , and ), previously misidentified as or by biochemical methods. Globally, we report that commensal strains present virulence traits (virulence genes, siderophores and biofilms) comparable to community-acquired and NICU-infective isolates, thus suggesting that the human microbiota could constitute a reservoir for infection. Isolates causing NICU outbreak were multi-drug resistant (MDR) and ESBLs producers, although an imipenem-resistant commensal MDR isolate was also found. A commensal strain showed a potent bacteriocin-like inhibitory activity against MDR isolates, thus highlighting the potential role of commensal spp. in health and disease.
PubMed: 34835469
DOI: 10.3390/microorganisms9112344 -
European Journal of Gastroenterology &... Jul 2023Incidence of ulcerative colitis is globally increased. Enteric infections and their role in ulcerative colitis flares present a common health problem and a unique... (Observational Study)
Observational Study
OBJECTIVE
Incidence of ulcerative colitis is globally increased. Enteric infections and their role in ulcerative colitis flares present a common health problem and a unique clinical challenge. We aimed to identify enteropathogens in flared ulcerative colitis patients and their antimicrobial susceptibilities and relation with the disease activity.
METHODS
Stool samples were collected from 95 patients with ulcerative colitis (17 inactive cases and 78 active cases) according to the Mayo score assessment of ulcerative colitis severity. Enteropathogens were examined using an automated VITEK2 system and FilmArray gastrointestinal pathogen panel.
RESULTS
Enteric infections were found in 81 patients (85.3%) with a significantly higher percentage in active ulcerative colitis (96.2% vs. 35.3%, P < 0.001). In 78 symptomatic patients, (78.7%) of bacteria as enteroaggregative and enteropathogenic E. coli , (11.5%) parasitic as Cryptosporidium and (7.7%) viral as Norovirus were the most detected microbial pathogens. Mixed, multidrug-resistant organisms (MDROs) and opportunistic infections were reported in 70.7%, 52.9% and 46.7% respectively. Raoultella ornithinolytica was reported for the first time as an enteropathogen in ulcerative colitis flare. Multiple organisms, MDROs, extended-spectrum beta-lactamases-producing and AmpC-resistant bacteria were significantly associated with disease severity.
CONCLUSION
Identifying enteropathogens especially opportunistic and MDR organisms as a cause of ulcerative colitis flare-ups is a matter of worry increasing their diagnostic and therapeutic burden. Periodic studies evaluating changes in microbial profiles and their antimicrobial susceptibilities are needed to achieve antibiotic stewardship and improve management.
Topics: Humans; Colitis, Ulcerative; Escherichia coli; Cryptosporidiosis; Feces; Cryptosporidium; Bacteria; Anti-Infective Agents
PubMed: 37161967
DOI: 10.1097/MEG.0000000000002563 -
Biotechnology Reports (Amsterdam,... Dec 2017Interest in novel ligninolytic bacteria has remained topical due to, in part, the maneuverability of the bacterial genome. Conversely, the fungal genome lacks the...
Interest in novel ligninolytic bacteria has remained topical due to, in part, the maneuverability of the bacterial genome. Conversely, the fungal genome lacks the dexterity for similar maneuverability thus, posing challenges in the fungal enzyme yield optimization process. Some impact of this situation includes the inability to commercialize the bio-catalytic process of lignin degradation by fungi. Consequently, this study assessed some fresh water bacteria isolates for ligninolytic and peroxidase properties through the utilization and degradation of model lignin compounds (guaiacol and veratryl alcohol) and the decolourization of selected ligninolytic indicator dyes; Azure B (AZB), Remazol Brilliant Blue R (RBBR) and Congo Red (CR). Bacterial strains with appreciable ligninolytic and peroxidase production potentials were identified through 16S rDNA sequence analysis and the nucleotide sequences deposited in the GenBank. About 5 isolates were positive for the degradation of both guaiacol (GA) and veratryl alcohol (VA) thus, accounting for about 17% of the test isolates. Similarly, AZB, RBBR and CR were respectively decolorized by 3, 2 and 5 bacterial strains thus, accounting for 10%, 7% and 17% of the test isolates. Two of the test bacterial strains were able to decolourize AZB, RBBR and CR respectively and these bacterial strains were identified as OKOH-1 and NWODO-2 with respective accession numbers as KX640917 and KX640918. Upon quantitation of the peroxidase activities; 5250 ± 0.00 U/L was recorded against OKOH-1 and 5833 ± 0.00 U/L against NWODO-2. The ligninolytic and dye decolourization properties of OKOH-1 and NWODO-2 marks for novelty particularly, as dyes with arene substituents were decolourized. Consequently, the potentials for the industrial applicability of these test bacterial strains abound as there is a dearth of information on organisms with such potentials.
PubMed: 29062721
DOI: 10.1016/j.btre.2017.10.001 -
Journal of Dairy Science Mar 2021Milk fan is a cheese-like fermented milk product produced in Yunnan Province, China. In this study, we characterized the microbial communities of milk fan from 6... (Review)
Review
Milk fan is a cheese-like fermented milk product produced in Yunnan Province, China. In this study, we characterized the microbial communities of milk fan from 6 distinct geographical origins and investigated their generation of volatile organic compounds (VOC). The microbial communities found in all milk fan samples were dominated by Lactococcus, Lactobacillus, and Raoultella bacteria and Rhodotorula, Torulaspora, and Candida fungi. Samples from the Kunming and Weishan regions had greater bacterial richness, and samples from Xizhou had greater fungal community richness. Sixty prominent VOC (i.e., those having odor activity values ≥1), including esters, acids, alcohols, aldehydes, ketones, and aromatic compounds, were identified by gas chromatography-mass spectrometry analysis of milk fan samples. Pearson correlation analysis revealed that Lactobacillus, Rhodotorula, Lodderomyces, and Debaryomyces had significant correlations with various VOC, revealing a total of 13 compounds that are characteristic of the odor of milk fan. These bacteria and fungi are therefore identified as functional microorganisms that collectively create the complex VOC profile of milk fan. This study provides a comprehensive overview of the microbial community of milk fan and demonstrates its contribution to the unique aroma profile of this fermented milk product.
Topics: Animals; Cheese; China; Microbiota; Milk; Volatile Organic Compounds
PubMed: 33358802
DOI: 10.3168/jds.2020-19053 -
Revista Espanola de Anestesiologia Y... Feb 2018Infections in humans by Raoultella spp. are rare and the effect that Raoultella spp. might have as a human pathogen is currently unknown. A clinical case is presented of...
Infections in humans by Raoultella spp. are rare and the effect that Raoultella spp. might have as a human pathogen is currently unknown. A clinical case is presented of catheter-related bacteraemia caused by Raoultella ornithinolytica, treated successfully with applied antibiotic therapy (intravenous cefepime and sealed the catheter with amikacin).
Topics: Aged; Amikacin; Anti-Bacterial Agents; Antineoplastic Combined Chemotherapy Protocols; Bacteremia; Catheter-Related Infections; Cefepime; Combined Modality Therapy; Drug Resistance, Multiple, Bacterial; Enterobacteriaceae; Enterobacteriaceae Infections; Equipment Contamination; Female; Humans; Pancreatic Neoplasms; Postoperative Complications
PubMed: 29162280
DOI: 10.1016/j.redar.2017.09.007