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Results and Problems in Cell... 2017Our understanding of the functions of vitellogenin (Vtg) in reproduction has undergone an evolutionary transformation over the past decade. Primarily, Vtg was regarded... (Review)
Review
Our understanding of the functions of vitellogenin (Vtg) in reproduction has undergone an evolutionary transformation over the past decade. Primarily, Vtg was regarded as a female-specific reproductive protein, which is cleaved into yolk proteins including phosvitin (Pv) and lipovitellin (Lv), stored in eggs, providing the nutrients for early embryos. Recently, Vtg has been shown to be an immunocomponent factor capable of protecting the host against the attack by microbes including bacteria and viruses. Moreover, Pv and Lv that both are proteolytically cleaved products of maternal Vtg, as well as Pv-derived small peptides, all display an antibacterial role in developing embryos. In addition, both Vtg and yolk protein Pv possess antioxidant activity capable of protecting cells from damage by free radicals. Collectively, these data indicate that Vtg, in addition to being involved in yolk protein formation, also plays non-nutritional roles via functioning as immune-relevant molecules and antioxidant reagents.
Topics: Animals; Egg Proteins; Female; Humans; Oocytes; Phosvitin; Vitellogenins
PubMed: 28779327
DOI: 10.1007/978-3-319-60855-6_17 -
Comparative Biochemistry and... May 2021Most broadcast spawner corals have a vitellogenic phase that lasts at least 6 months. It is established that estrogen regulates vitellogenin synthesis in vertebrates....
Most broadcast spawner corals have a vitellogenic phase that lasts at least 6 months. It is established that estrogen regulates vitellogenin synthesis in vertebrates. Although some research have been conducted on the physiological role of sex steroids in corals, little is known about their involvement in oocyte development. This study aimed to detect steroid hormones - progesterone, testosterone, and estradiol-17β (E2) - in Acropora tenuis and study the relationships between vitellogenesis/vitellogenin synthesis and these steroids. This study also investigated the effect of E2 on vitellogenin synthesis in corals and identified steroidogenic enzymes in A. tenuis genome. Branches from tagged coral colonies were collected monthly from March to November. Histological observations showed that oocytes were vitellogenic from March to May (Stage IV and V), but not in June, and that gonads were occupied by immature oocytes in September (Stage I). Real-time qPCR revealed that vitellogenin (vg1 and vg2) transcript levels in coral branches were high in April and May, implying that corals actively underwent vitellogenesis during these months, and spawned before June. Liquid chromatography-mass spectrometry revealed that E2 could be detected in coral branches in March, April, and May, but not in June, whereas testosterone and progesterone did not fluctuate much in the same months. Immersing branches in E2-containing seawater failed to increase vitellogenin transcript levels. The results indicate that E2 is involved in oogenesis but does not positively regulate vitellogenin synthesis. Steroidogenic enzymes (except CYP19A) were identified in A. tenuis, suggesting that corals may endogenously synthesize progestogens and androgens from cholesterol.
Topics: Animals; Anthozoa; Chromatography, Liquid; Cloning, Molecular; Estradiol; Mass Spectrometry; Oocytes; Oogenesis; Progesterone; RNA, Messenger; Testosterone; Vitellogenins
PubMed: 33486078
DOI: 10.1016/j.cbpa.2021.110910 -
Journal of Insect Science (Online) Nov 2020Oviposition-related genes have remained a consistent focus of insect molecular biology. Previous research has gradually clarified our mechanistic understanding of... (Review)
Review
Oviposition-related genes have remained a consistent focus of insect molecular biology. Previous research has gradually clarified our mechanistic understanding of oviposition-related genes, including those related to oviposition-gland-related genes, oogenesis-related genes, oviposition-site-selection-related genes, and genes related to ovulation and hatching. Moreover, some of this research has revealed how the expression of single oviposition-related genes affects the expression of related genes, and more importantly, how individual node genes function to link the expression of upstream and downstream genes. However, the research to date is not sufficient to completely explain the overall interactions among the genes of the insect oviposition system. Through a literature review of a large number of studies, this review provides references for future research on oviposition-related genes in insects and the use of RNAi or CRISPR/Cas9 technology to verify the functions of oviposition-related genes and to prevent and control harmful insects.
Topics: Animals; CRISPR-Cas Systems; Egg Proteins; Female; Gene Expression; Genes, Insect; Insect Control; Insecta; Oogenesis; Oviposition; RNA Interference; Receptors, Cell Surface; Vitellogenins
PubMed: 33367730
DOI: 10.1093/jisesa/ieaa137 -
MBio Feb 2023Integration between animal reproduction and symbiont inheritance is fundamental in symbiosis biology, but the underlying molecular mechanisms are largely unknown....
Integration between animal reproduction and symbiont inheritance is fundamental in symbiosis biology, but the underlying molecular mechanisms are largely unknown. Vitellogenin (Vg) is critical for oogenesis, and it is also a pathogen pattern recognition molecule in some animals. Previous studies have shown that Vg is involved in the regulation of symbiont abundance and transmission. However, the mechanisms by which an insect and its symbiont contribute to the function of Vg and how Vg impacts the persistence of insect-microbe symbiosis remain largely unclear. Symbionts are transovarially transmitted via maternal inheritance of the bacteriocytes in the whitefly Bemisia tabaci. Surprisingly, Vg is localized in bacteriocytes of whiteflies. Vg could be synthesized in whitefly bacteriocytes by the gene expressed in these cells or exported into bacteriocytes from hemolymph via the Vg receptor. We further found that the juvenile hormone and " Portiera aleyrodidarum" (here termed ) control the level and localization of Vg in whiteflies. Immunocapture PCR revealed interactions between Vg and . Suppressing Vg expression reduced abundance as well as whitefly oogenesis and fecundity. Thus, we reveal that Vg facilitated the persistence of whitefly-bacteriocyte symbiont associations. This study will provide insight into the key role of Vg in the coevolution of insect reproduction and symbiont inheritance. Intracellular heritable symbionts have been incorporated into insect reproductive and developmental biology by various mechanisms. All Bemisia tabaci species harbor the obligate symbiont in specialized insect cells called bacteriocytes. We report that the whitefly juvenile hormone and determined vitellogenin (Vg) localization in bacteriocytes of whiteflies. In turn, Vg affected whitefly fecundity as well as fitness and transmission of the symbiont. Our findings show that Vg, a multifunctional protein, is indispensable for symbiont integration into the reproduction and development of insects. This reflects the outcome of long-term coevolution of the insect-microbe symbiosis.
Topics: Animals; Vitellogenins; Hemiptera; Symbiosis; Polymerase Chain Reaction
PubMed: 36692332
DOI: 10.1128/mbio.02990-22 -
Nutrients Oct 2015Vitellogenin (Vtg), the major egg yolk precursor protein, is traditionally thought to provide protein- and lipid-rich nutrients for developing embryos and larvae.... (Review)
Review
Vitellogenin (Vtg), the major egg yolk precursor protein, is traditionally thought to provide protein- and lipid-rich nutrients for developing embryos and larvae. However, the roles of Vtg as well as its derived yolk proteins lipovitellin (Lv) and phosvitin (Pv) extend beyond nutritional functions. Accumulating data have demonstrated that Vtg, Lv and Pv participate in host innate immune defense with multifaceted functions. They can all act as multivalent pattern recognition receptors capable of identifying invading microbes. Vtg and Pv can also act as immune effectors capable of killing bacteria and virus. Moreover, Vtg and Lv are shown to possess phagocytosis-promoting activity as opsonins. In addition to these immune-relevant functions, Vtg and Pv are found to have antioxidant activity, which is able to protect the host from oxidant stress. These non-nutritional functions clearly deepen our understanding of the physiological roles of the molecules, and at the same time, provide a sound basis for potential application of the molecules in human health.
Topics: Animals; Antioxidants; Bacteria; Egg Proteins; Fishes; Phosvitin; Receptors, Pattern Recognition; Viruses; Vitellogenins
PubMed: 26506386
DOI: 10.3390/nu7105432 -
PLoS Pathogens Dec 2023Microsporidia are a group of obligate intracellular parasites that infect almost all animals, causing serious human diseases and major economic losses to the farming...
Microsporidia are a group of obligate intracellular parasites that infect almost all animals, causing serious human diseases and major economic losses to the farming industry. Nosema bombycis is a typical microsporidium that infects multiple lepidopteran insects via fecal-oral and transovarial transmission (TOT); however, the underlying TOT processes and mechanisms remain unknown. Here, we characterized the TOT process and identified key factors enabling N. bombycis to invade the ovariole and oocyte of silkworm Bombyx mori. We found that the parasites commenced with TOT at the early pupal stage when ovarioles penetrated the ovary wall and were exposed to the hemolymph. Subsequently, the parasites in hemolymph and hemolymph cells firstly infiltrated the ovariole sheath, from where they invaded the oocyte via two routes: (I) infecting follicular cells, thereby penetrating oocytes after proliferation, and (II) infecting nurse cells, thus entering oocytes following replication. In follicle and nurse cells, the parasites restructured and built large vacuoles to deliver themselves into the oocyte. In the whole process, the parasites were coated with B. mori vitellogenin (BmVg) on their surfaces. To investigate the BmVg effects on TOT, we suppressed its expression and found a dramatic decrease of pathogen load in both ovarioles and eggs, suggesting that BmVg plays a crucial role in the TOT. Thereby, we identified the BmVg domains and parasite spore wall proteins (SWPs) mediating the interaction, and demonstrated that the von Willebrand domain (VWD) interacted with SWP12, SWP26 and SWP30, and the unknown function domain (DUF1943) bound with the SWP30. When disrupting these interactions, we found significant reductions of the pathogen load in both ovarioles and eggs, suggesting that the interplays between BmVg and SWPs were vital for the TOT. In conclusion, our study has elucidated key aspects about the microsporidian TOT and revealed the key factors for understanding the molecular mechanisms underlying this transmission.
Topics: Animals; Humans; Vitellogenins; Spores, Fungal; Nosema; Bombyx
PubMed: 38060601
DOI: 10.1371/journal.ppat.1011859 -
Toxicology Jun 2022In view of the recurrent applications of pesticides in agricultural producing countries, the increased presence of these substances in the environment raise a demand for...
In view of the recurrent applications of pesticides in agricultural producing countries, the increased presence of these substances in the environment raise a demand for the evaluation of adverse effects on non-target organisms. This study assesses the impact of exposure to five pesticides suspected of being endocrine disruptors (atrazine, 2,4-dichlorophenoxyacetic acid, mancozeb, chlorpyrifos and cypermethrin) on the reproductive development of the nematode Caenorhabditis elegans. To this end, nematodes in the L4 larval stage were exposed to different concentrations of pesticides for 24 h and the consequences on brood size, percentage of gravid nematodes, expression of reproductive-related genes and vitellogenin trafficking and endocytosis were measured. Moreover, 17β-estradiol was used as an estrogenic control for endocrine disrupting compounds throughout the work. The results showed that all the pesticides disturbed to some extent one or more of the evaluated endpoints. Remarkably, we found that atrazine, 2,4-dichlorophenoxyacetic acid and chlorpyrifos produced comparable responses to 17β-estradiol suggesting that these pesticides may have estrogen-like endocrine disrupting activity. Atrazine and 17β-estradiol, as well as 2,4-dichlorophenoxyacetic acid and chlorpyrifos to a lesser extent, decreased the brood size, affected vitellogenin trafficking and endocytosis, and changed the expression of several reproductive-related genes. Conversely, mancozeb and cypermethrin had the least impact on the evaluated endpoint. Cypermethrin affected the brood size at the highest concentration tested and mancozeb altered the distribution of vitellogenin only in approximately 10% of the population. However, both products overexpressed hus-1 and vit-2 genes, indicating that an induction of stress could interfere with the normal development of the nematode. In conclusion, our work proved that C. elegans is a useful biological model to identify the effects of estrogen-like endocrine disruptor compounds, and the sublethal endpoints proposed may serve as an important contribution on evaluating environmental pollutants.
Topics: 2,4-Dichlorophenoxyacetic Acid; Animals; Atrazine; Caenorhabditis elegans; Chlorpyrifos; Endocrine Disruptors; Estradiol; Estrogens; Herbicides; Pesticides; Vitellogenins
PubMed: 35697162
DOI: 10.1016/j.tox.2022.153229 -
PloS One 2016Vitellogenin (Vg) plays vital role in oocytes and embryo development in insects. Vg is synthesized in the fat body, moves through haemolymph and accumulates in oocytes....
Vitellogenin (Vg) plays vital role in oocytes and embryo development in insects. Vg is synthesized in the fat body, moves through haemolymph and accumulates in oocytes. Vitellogenin receptors (VgR) present on the surface of oocytes, are responsible for Vg transportation from haemolymph to oocytes. Here, we cloned and characterized these genes from Bemisia tabaci Asia1 (BtA1) species. The cloned BtA1Vg and BtA1VgR genes consisted of 6,330 and 5,430 bp long open reading frames, which encoded 2,109 and 1,809 amino acid (AA) residues long protein. The BtA1Vg protein comprised LPD_N, DUF1943 and VWFD domains, typical R/KXXR/K, DGXR and GL/ICG motifs, and polyserine tracts. BtA1VgR protein contained 12 LDLa, 10 LDLb and 7 EGF domains, and a trans-membrane and cytoplasmic region at C-terminus. Phylogenetic analyses indicated evolutionary association of BtA1Vg and BtA1VgR with the homologous proteins from various insect species. Silencing of BtA1VgR by siRNA did not affect the transcript level of BtA1Vg. However, BtA1Vg protein accumulation in oocytes was directly influenced with the expression level of BtA1VgR. Further, BtA1VgR silencing caused significant mortality and reduced fecundity in adult whiteflies. The results established the role of BtA1VgR in transportation of BtA1Vg in oocytes. Further, these proteins are essential for fecundity, and therefore these can be potential RNAi targets for insect control in crop plants.
Topics: Amino Acid Sequence; Animals; Data Mining; Diptera; Egg Proteins; Phylogeny; Receptors, Cell Surface; Sequence Homology, Amino Acid; Vitellogenins
PubMed: 27159161
DOI: 10.1371/journal.pone.0155306 -
Experimental & Applied Acarology Mar 2022Vitellogenin (Vg) is an important factor that impacts oocyte maturation, egg formation and embryonic development in Arthropoda. Two orthologs of Vg gene were obtained...
Vitellogenin (Vg) is an important factor that impacts oocyte maturation, egg formation and embryonic development in Arthropoda. Two orthologs of Vg gene were obtained from the genome of Phytoseiulus persimilis and termed as PpVg1 and PpVg2. Both orthologs belong to the large lipid transfer protein superfamily. The expression of PpVg1 and PpVg2 was low in immatures and male adults, and increased rapidly in female adults after mating, and reached a peak before the first egg was laid (168× and 20.5× the level in virgin females, respectively). When PpVg1 and PpVg2 were interfered with dsRNA, the relative expression decreased by 81.0 and 30.9%, respectively, and 7.8 and 31.4% interfered individuals died within 24 h. Among surviving individuals, ca. 51.1 and 44.8% are infertile. Factors that might be related to expression of Vg genes are also discussed.
Topics: Animals; Female; Male; Mites; Reproduction; Vitellogenins
PubMed: 35239074
DOI: 10.1007/s10493-022-00698-8 -
Pest Management Science Apr 2018The beet armyworm Spodoptera exigua, a worldwide phytophagous pest, causes considerable economic agricultural losses. Understanding the relationship between its...
BACKGROUND
The beet armyworm Spodoptera exigua, a worldwide phytophagous pest, causes considerable economic agricultural losses. Understanding the relationship between its fecundity and the host plant is a basic and important component of early forecasting of beet armyworm outbreaks. However, little is known about the molecular mechanism by which distinct hosts affect S. exigua fecundity.
RESULTS
In this study, key life-history parameters of S. exigua reared on distinct hosts were investigated; the host plants could be ranked as lettuce > shallot > tomato > celery in their order of suitability. Full-length S. exigua vitellogenin receptor (SeVgR) cDNA was cloned, and sex-, stage- and tissue-specific expression characteristics were assessed. Spodoptera exigua vitellogenin (SeVg) and SeVgR expression levels were markedly modulated by host nutrients (P < 0.05). SeVg and SeVgR expression levels were significantly higher in S. exigua reared on lettuce, the most preferred and most nutritive host, than in those reared on tomato and celery. Interestingly, significant linear regression correlations were found between SeVg and SeVgR expression levels and key S. exigua life-history parameters, especially life span, pupa weight, and female fecundity (P < 0.01).
CONCLUSION
Host plant type and suitability could affect the expression pattern of SeVg and SeVgR, which influenced S. exigua fecundity. Vg and VgR have the potential to be used as molecular markers of S. exigua fecundity and for forecasting outbreaks of S. exigua on different hosts. © 2017 Society of Chemical Industry.
Topics: Animals; Egg Proteins; Female; Fertility; Food Chain; Herbivory; Insect Proteins; Larva; Life History Traits; Receptors, Cell Surface; Spodoptera; Transcriptome; Vitellogenins
PubMed: 29149486
DOI: 10.1002/ps.4794