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Fish & Shellfish Immunology Oct 2015Our understanding of the function of vitellogenin (Vg) in reproduction has undergone a transformation over the past decade in parallel with new insights into the role of... (Review)
Review
Our understanding of the function of vitellogenin (Vg) in reproduction has undergone a transformation over the past decade in parallel with new insights into the role of Vg in immunity. Initially, Vg was regarded as a female-specific reproductive protein, which is cleaved into yolk proteins such as phosvitin (Pv) and lipovitellin (Lv), stored in egg, providing the nutrients for developing embryos. Recently, Vg is shown to be an immune-relevant molecule involved in the defense of the host against the microbes including bacterium and virus. Furthermore, Pv and Lv, that both are proteolytically cleaved products of Vg, play a defense role in developing embryos. Importantly, yolk protein-derived small peptides also display antimicrobial activity. These data together indicate that Vg, in addition to being involved in yolk protein formation, plays a non-reproductive role via functioning as an immune-relevant molecule in both parent fishes and their offspring. It also shows that yolk proteins and their degraded peptides are novel players in maternal immunity, opening a new avenue to study the functions of reproductive proteins.
Topics: Animals; Egg Proteins; Fish Proteins; Fishes; Immunocompetence; Vitellogenins
PubMed: 26282682
DOI: 10.1016/j.fsi.2015.08.011 -
Microbiology Spectrum Aug 2022Liberibacter solanacearum (CLso) haplotype D, transmitted by the carrot psyllid , is a major constraint for carrot production in Israel. Unveiling the molecular...
Liberibacter solanacearum (CLso) haplotype D, transmitted by the carrot psyllid , is a major constraint for carrot production in Israel. Unveiling the molecular interactions between the psyllid vector and CLso can facilitate the development of nonchemical approaches for controlling the disease caused by CLso. Bacterial surface proteins are often known to be involved in adhesion and virulence; however, interactions of CLso with carrot psyllid proteins that have a role in the transmission process has remained unexplored. In this study, we used CLso outer membrane protein (OmpA) and flagellin as baits to screen for psyllid interacting proteins in a yeast two-hybrid system assay. We identified psyllid vitellogenin (Vg) to interact with both OmpA and flagellin of CLso. As Vg and autophagy are often tightly linked, we also studied the expression of autophagy-related genes to further elucidate this interaction. We used the juvenile hormone (JH-III) to induce the expression of Vg, thapsigargin for suppressing autophagy, and rapamycin for inducing autophagy. The results revealed that Vg negatively regulates autophagy. Induced Vg expression significantly suppressed autophagy-related gene expression and the levels of CLso significantly increased, resulting in a significant mortality of the insect. Although the specific role of Vg remains obscure, the findings presented here identify Vg as an important component in the insect immune responses against CLso and may help in understanding the initial molecular response in the vector against Liberibacter. Pathogen transmission by vectors involves multiple levels of interactions, and for the transmission of liberibacter species by psyllid vectors, much of these interactions are yet to be explored. Liberibacter solanacearum (CLso) haplotype D inflicts severe economic losses to the carrot industry. Understanding the specific interactions at different stages of infection is hence fundamental and could lead to the development of better management strategies to disrupt the transmission of the bacteria to new host plants. Here, we show that two liberibacter membrane proteins interact with psyllid vitellogenin and also induce autophagy. Altering vitellogenin expression directly influences autophagy and CLso abundance in the psyllid vector. Although the exact mechanism underlying this interaction remains unclear, this study highlights the importance of immune responses in the transmission of this disease agent.
Topics: Animals; Autophagy; Flagellin; Hemiptera; Liberibacter; Plant Diseases; Rhizobiaceae; Vitellogenins
PubMed: 35863005
DOI: 10.1128/spectrum.01577-22 -
International Journal of Molecular... Oct 2022The fall armyworm is a highly polyphagous invasive pest. The strong reproductive capacity is an important factor in the rapid colonization and expansion of...
The fall armyworm is a highly polyphagous invasive pest. The strong reproductive capacity is an important factor in the rapid colonization and expansion of Vitellogenin (Vg) and vitellogenin receptor (VgR) play important roles in insect reproduction. As the precursor of vitellin (Vn), Vg provides essential nutrition for embryonic development, and VgR mediates the uptake of Vg by oocytes. In this context, we cloned and characterized these two genes of ( and ) and evaluated their expression profiles in different developmental stages and tissues. The RNA interference experiment was used to investigate their function in vitellogenesis. The ORF values of and were 5250 and 5445 bp, encoding 1749 and 1815 amino acid residues, respectively. The qRT-PCR results revealed that both and were highly expressed in female adults; was specifically expressed in the fat body, whereas was highly expressed in the ovary. In addition, the depletion of either or hindered oocyte maturation and ovarian development, leading to a significant decrease in fecundity. The present study reveals the importance of and in the vitellogenesis of , laying a theoretical foundation for the development of pollution-free pest control strategies with and as new targets.
Topics: Amino Acids; Animals; Female; Spodoptera; Vitellins; Vitellogenesis; Vitellogenins
PubMed: 36233286
DOI: 10.3390/ijms231911972 -
Animal Reproduction Science Dec 2020To evaluate functions of vitellogenin (Vg) and heat shock protein 90 (Hsp90) during ovarian and embryonic development of Exopalaemon carinicauda, the cDNA of Vg (Ec-Vg)...
To evaluate functions of vitellogenin (Vg) and heat shock protein 90 (Hsp90) during ovarian and embryonic development of Exopalaemon carinicauda, the cDNA of Vg (Ec-Vg) was cloned, and the abundances of Ec-Vg and heat shock protein 90 (Hsp90) (Ec-Hsp90) were determined during ovarian and embryonic development. During ovarian development, the concentration of Vg protein in hemolymph was markedly greater than in the ovary and hepatopancreas, and was greatest at sexual maturity. The relative abundance of Ec-Vg mRNA transcript was greatest in the hepatopancreas and almost undetectable in hemocytes. By combining mRNA transcript relative abundances with morphological results, a model was developed to explain the mRNA transcript relative abundance of Ec-Hsp90 and Ec-Vg during ovarian and embryonic development. The relative abundance of Ec-Vg mRNA transcript was greatest during the recovery period after reproduction had occurred (Stage V) and mature stage (Stage IV) in the ovary or hepatopancreas, respectively. There were marked associations of the patterns of Ec-Hsp90 and Ec-Vg mRNA transcript abundances both in hepatopancreas and ovary. During embryonic development, the relative abundance of the two mRNA transcripts were greatest at the metazoea and protozoea stages, respectively. These results indicate that Ec-Vg is produced primarily in the hepatopancreas, secreted into the hemolymph and transported into growing oocytes. It, therefore, is believed that Ec-Vg has an important function in the overall ovarian development and late embryonic development of E. carinicauda. In contrast, Ec-Hsp90 is a regulatory factor for Vg transcription and is important during early organogenesis in E. carinicauda.
Topics: Animals; Decapoda; Female; Gene Expression Regulation, Developmental; HSP90 Heat-Shock Proteins; Ovary; Vitellogenins
PubMed: 33099104
DOI: 10.1016/j.anireprosci.2020.106633 -
Aging Cell Nov 2022Some of the most conspicuous aging phenotypes of C. elegans are related to post-reproductive production of vitellogenins (Vtg), which form yolk protein (YP) complexes...
Some of the most conspicuous aging phenotypes of C. elegans are related to post-reproductive production of vitellogenins (Vtg), which form yolk protein (YP) complexes after processing and lipid loading. Vtg/YP levels show huge increases with age, and inhibition of this extends lifespan, but how subcellular and organism-wide distribution of these proteins changes with age has not been systematically explored. Here, this has been done to understand how vitellogenesis promotes aging. The age-associated changes of intestinal vitellogenin vesicles (VVs), pseudocoelomic yolk patches (PYPs), and gonadal yolk organelles (YOs) have been characterized by immuno-electron microscopy. We find that from reproductive adult day 2 (AD 2) to post-reproductive AD 6 and AD 9, intestinal VVs expand from 0.2 to 3-4 μm in diameter or by >3000 times in volume, PYPs increase by >3 times in YP concentration and volume, while YOs in oocytes shrink slightly from 0.5 to 0.4 μm in diameter or by 49% in volume. In AD 6 and AD 9 worms, mislocalized YOs found in the hypodermis, uterine cells, and the somatic gonadal sheath can reach a size of 10 μm across in the former two tissues. This remarkable size increase of VVs and that of mislocalized YOs in post-reproductive worms are accompanied by extensive fusion between these Vtg/YP-containing vesicular structures in somatic cells. In contrast, no fusion is seen between YOs in oocytes. We propose that in addition to the continued production of Vtg, excessive fusion between VVs and mislocalized YOs in the soma worsen the aging pathologies seen in C. elegans.
Topics: Animals; Vitellogenins; Caenorhabditis elegans; Vitellogenesis; Egg Proteins; Oocytes
PubMed: 36199214
DOI: 10.1111/acel.13719 -
Reproductive Toxicology (Elmsford, N.Y.) Aug 2023Di-(2-ethylhexyl) terephthalate (DEHTP) is frequently used in food packaging and medical devices as an alternative to di-(2-ethylhexyl) phthalate (DEHP). In this study,...
Di-(2-ethylhexyl) terephthalate (DEHTP) is frequently used in food packaging and medical devices as an alternative to di-(2-ethylhexyl) phthalate (DEHP). In this study, zebrafish pairs were exposed to DEHTP for 21 d and the effects on fertility, sex hormone levels, vitellogenin levels, and transcription of genes along the hypothalamic-pituitary-gonad axis were evaluated. Results showed that mean egg numbers were significantly reduced in the 30 and 300 μg/L DEHTP groups. The adverse effects of DEHTP on hormones and gene transcripts were more prominent in males than in females. In male fish, the gonadosomatic index, hepatosomatic index, and vitellogenin concentration were significantly increased. The results of a significant decrease in testosterone (T) and an increase in the 17β-estradiol (E2)/T ratio in males exposed to 3-300 μg/L DEHTP suggest that the endocrine potential of DEHTP is similar that of DEHP. In females, genes related to gonadotropin-releasing hormone and gonadotropin were up-regulated while E2 was significantly down-regulated. These findings suggest that positive E2 feedback mechanisms in the hypothalamus and pituitary gland are activated to balance sex hormones. The effects of chronic exposure to DEHTP on the neuroendocrine system require further investigation.
Topics: Female; Animals; Male; Zebrafish; Diethylhexyl Phthalate; Vitellogenins; Gonadal Steroid Hormones; Gonads; Water Pollutants, Chemical
PubMed: 37211339
DOI: 10.1016/j.reprotox.2023.108408 -
Aging Oct 2021Eggs are rich in nutrients and contain a lot of protein. Although eggs have proved to accelerate the growth of C2C12 cells, the regulatory and mechanism of fertilized...
Eggs are rich in nutrients and contain a lot of protein. Although eggs have proved to accelerate the growth of C2C12 cells, the regulatory and mechanism of fertilized egg yolk extract (FEYE) on skeletal muscle development and fat metabolism remains unclearly. The mice were treated with FEYE by gavage for 24 d, we found that FEYE can inhibit the expression of skeletal muscle atrophy genes such as MSTN and Murf-1, and up-regulate the expression levels of MYOD, MYOG and Irisin. In addition, the treatment of FEYE induced UCP1 and PGC1α high expression in WAT, thereby causing WAT browning reaction. In order to confirm the composition of FEYE, we performed protein full spectrum identification (LC MS/MS) analysis and found the most enriched component is vitellogenin 2 (VTG2). Therefore, we added the recombinant protein VTG2 to C2C12 cells and found that VTG2 promoted the proliferation and differentiation of C2C12 cells. After that, we further proved that VTG2 inhibited the expression of MSTN and improved the expression of MYOD and Irisin. Finally, the dual luciferase test proved that VTG2 directly inhibited the transcriptional activity of MSTN. Our results conclude that FEYE inhibits the expression of MSTN in muscle tissues by delivering VTG2, thereby promoting skeletal muscle development, and can also promote the expression level of FNDC5 in serum. Then, FNDC5 acts on the fat through the serum, stimulating the browning reaction of white adipocytes. Therefore, VTG2 can be used to stop muscle consumption, improve skeletal muscle aging, and prevent obesity.
Topics: Adipose Tissue, Brown; Adipose Tissue, White; Animals; Cell Differentiation; Cell Line; Cell Proliferation; Egg Yolk; Lipid Metabolism; Male; Mice; Mice, Inbred C57BL; Muscle, Skeletal; Myostatin; Tissue Extracts; Vitellogenins
PubMed: 34609951
DOI: 10.18632/aging.203590 -
Journal of Fish Biology Feb 2022Providing a non-invasive procedure to track fish maturity remains a priority in broodstocks' management. In the present study, the main goal was to assess reproduction... (Comparative Study)
Comparative Study
Comparative study on accuracy of mucosal estradiol-17β, testosterone and 11-ketotestosterone, for maturity, and cutaneous vitellogenin gene expression in goldfish (Carassius auratus).
Providing a non-invasive procedure to track fish maturity remains a priority in broodstocks' management. In the present study, the main goal was to assess reproduction status by measuring sex steroids and vitellogenin (VTG) in the skin mucosa, as a non-invasive method. For this purpose, the present study compared the levels of estradiol-17β (E ), testosterone (T), 11-ketotestosterone (11-KT), VTG and calcium (Ca) in skin mucosa and blood plasma of goldfish (Carassius auratus). Skin mucosal and blood samples were collected, as well as gonad tissues, from goldfish, as a seasonal spawner. Histological analysis confirmed the gender and maturity status from females' ovaries (as primary-growth, cortical-alveoli, initial and late-vitellogenesis) and males' testes (as spermatogenesis and spermiation). Furthermore, vitellogenin (vtg) expression was observed in skin, liver and gonads. The results indicate that mucosal E concentrations were significantly higher during initial and late vitellogenesis than the other stages. Mucosal 11-KT concentrations significantly increased at spermiation (P < 0.05). E /T and 11-KT/E ratios significantly increased at early vitellogenesis and spermatogenesis, respectively (P < 0.05). Females' mucosal VTG levels were significantly fluctuated according to the maturity stage. Ca showed a similar trend, but Ca was more accurate for sex identification than the VTG. Although mucus showed high levels of VTG, ovarian vtg expression was strongest while liver and skin had the similar results. These results show that measuring the mucosal androgens could be considered as an accurate, non-invasive method to monitor fish maturity.
Topics: Animals; Estradiol; Female; Gene Expression; Goldfish; Male; Mucous Membrane; Testosterone; Vitellogenins
PubMed: 34822181
DOI: 10.1111/jfb.14963 -
Ticks and Tick-borne Diseases May 2018Tick infestation in cattle reflects the main cause of economic loss to cattle producers. This is due to several reasons but mainly to their ability to feed on blood and...
Tick infestation in cattle reflects the main cause of economic loss to cattle producers. This is due to several reasons but mainly to their ability to feed on blood and generate a huge amount of eggs. Lipid transport in arthropods is achieved by highly specialized hemolymphatic lipoproteins, which resemble those described in vertebrate blood. Such lipoproteins continuously deliver lipids through the blood to growing eggs. The injection of radioactive [H] palmitic acid into tick hemocoel showed that the gut, ovary, fat body and Gene's organ were the main organs of incorporation of this labeled fatty acid. The rate of [H] palmitic acid incorporation by the organs was high up to 30 min after injection. The [H] palmitic acid incorporated by these organs was later found in phospholipids and neutral lipids. Here, we describe the purification and characterization of a key player of lipid dynamics in tick hemolymph. The Rhipicephalus microplus lipid-apolipoprotein complex (RmLCP) is a new high-density lipoprotein (1.18 g/mL), which accounts for over 90% of [H] palmitic acid present in the hemolymph. It has a native molecular weight of 420 kDa and is composed of one subunit of 122 kDa. Protein identification analysis of RmLPC subunit showed two better hits: vitellogenin 2 (23% protein coverage) and vitellogenin 5 (29% protein coverage), respectively and similarities with hemolymphatic apolipoproteins of arachnids such as the tick Ixodes scapularis (80%), the mite Galendromus occidentalis (44%) and the spider Parasteatoda tepidariorum (43%) and also for the insects Locusta migratoria (45%), Drosophila melanogaster (42%) and Manduca sexta (47%) to vitellogenin 2 and tick Ixodes scapularis (83%), the crab Limulus polyphemus (55%) and the oyster Crassostrea gigas (55%) to vitellogenin 5. Furthermore, it shows a distinct lipid composition from most arthropod lipoproteins, being composed of 40% free cholesterol, 27% phospholipids, 20% triacylglycerol and 15% hydrocarbons. In addition to binding most hemolymphatic fatty acids, this lipoprotein also binds and transports free cholesterol. In conclusion, the present study provides insight into the macromolecules involved in arachnid metabolism, which have significant potential for future use for the biological control of ticks.
Topics: Animals; Carrier Proteins; Cattle; Cattle Diseases; Cholesterol; Fatty Acids; Female; Ixodes; Lipoproteins; Phospholipids; Rhipicephalus; Tick Infestations; Vitellogenins
PubMed: 29567146
DOI: 10.1016/j.ttbdis.2018.03.010 -
General and Comparative Endocrinology Jan 2023The vitellogenin is composed by polypeptides that are precursors of egg yolk proteins that provides embryo and larvae nutrition. The mRNA encoding for vitellogenin Ab...
The vitellogenin is composed by polypeptides that are precursors of egg yolk proteins that provides embryo and larvae nutrition. The mRNA encoding for vitellogenin Ab (Vtg-Ab; 4,536 bp long and 1,512 amino acids) were obtained by RNA-Seq library sequencing of pirarucu gonads. The Vtg-Ab sequences had high homology with Vtgs of other three teleosts species of the order Osteoglossiformes. The transcript of ovarian Vtg was identified based on structural criteria, and so we classify the Vtg of pirarucu as Vtg-Ab due to the truncated or shortened phosvitin (N-terminal end) and phosvitinless domain (C-terminal end). The Vtg-Ab of pirarucu present two major deletions with 133 amino acids in the Lipovitellin I domain and 89 amino acids in the truncated or shortened Phosvitin domain, both located in the N-terminal end region. The three-dimensional (3-D) structure Vtg-Ab protein shows the presence of a typical 4α-helices bundle protein that runs in anti-parallel. In general, the characterization of Vtg-Ab may be the useful elucidation of the hormonal regulation of vitellogenesis and improve the production of pirarucu for broodstock management in aquaculture and preparation of Vtg antibody production (species-specific) for sex identification.
Topics: Animals; Vitellogenins; Phosvitin; Amino Acid Sequence; Fishes; Amino Acids
PubMed: 36244430
DOI: 10.1016/j.ygcen.2022.114143