-
Insect Science Feb 2023Oocyte maturation is critical for insect reproduction. Vitellogenesis, the timely production and uptake of vitellogenin (Vg), is crucial for female fecundity. Vg is...
Oocyte maturation is critical for insect reproduction. Vitellogenesis, the timely production and uptake of vitellogenin (Vg), is crucial for female fecundity. Vg is synthesized in fat body and absorbed by the oocytes through endocytosis during insect oogenesis. In the silkworm, Bombyx mori, we discovered that a nucleus-enriched long-noncoding RNA (lncRNA) lncR26319 regulates Endophilin A (EndoA) - a member of the endophilin family of endocytic proteins - through competitive binding to miR-2834. The lncR26319-miR-2834-EndoA axis was required for Vg endocytosis in the silkworm; loss of EndoA or overexpression of miR-2834 significantly reduced egg numbers in virgin moths. In addition, accumulation of miR-2834 resulted in pupal and adult deformation and reduced fecundity in females. The expression of Vg, 30-kDa (30K) protein, and egg-specific protein (Esp) decreased after knockdown of EndoA or overexpression of miR-2834, while knockdown of miR-2834 had an opposite effect on the expression of Vg, 30K protein gene, and Esp. These results suggest that the lncR26319-miR-2834-EndoA axis contributes to the endocytic activity in the Vg uptake and leads to the normal progression of oogenesis in the silkworm. Thus, miR-2834 and EndoA are crucial for female reproduction and could be potential targets for new pest management strategies in lepidopterans.
Topics: Female; Animals; Bombyx; Oogenesis; Vitellogenins; Oocytes; MicroRNAs; Insect Proteins
PubMed: 35612298
DOI: 10.1111/1744-7917.13082 -
The Journal of Experimental Biology Jan 2016In the ant Ectatomma tuberculatum (Olivier 1792), workers have active ovaries and lay trophic eggs that are eaten by the queen and larvae. Vitellogenins are the main...
In the ant Ectatomma tuberculatum (Olivier 1792), workers have active ovaries and lay trophic eggs that are eaten by the queen and larvae. Vitellogenins are the main proteins found in the eggs of insects and are the source of nutrients for the embryo in the fertilized eggs and for adults in the trophic eggs. In social insects, vitellogenin titres vary between castes and affect reproductive social status, nursing, foraging, longevity, somatic maintenance, and immunity. In most insects, vitellogenin synthesis is mainly regulated by juvenile hormone. However, in non-reproductive worker ants, this relationship is poorly characterized. This study determined the effects of juvenile hormone on vitellogenin synthesis in non-reproductive E. tuberculatum workers. Juvenile hormone was topically applied onto workers, and the effect on vitellogenin synthesis in the fat body and vitellogenin titres in the haemolymph were analysed by ELISA and qPCR. Juvenile hormone downregulated protein synthesis and reduced vitellogenin titres in the haemolymph, suggesting that in workers of E. tuberculatum, juvenile hormone loses its gonadotrophic function.
Topics: Animals; Ants; Fat Body; Female; Hemolymph; Juvenile Hormones; Ovum; Vitellogenins
PubMed: 26567344
DOI: 10.1242/jeb.127712 -
Reproduction, Fertility, and Development May 2021Vitellogenesis is essential for oocyte maturation. Vitellogenin (Vtg), a yolk precursor protein, plays an important role in oogenesis and vitellogenesis. Chinese hook...
Molecular cloning, characterisation and expression analysis of the vitellogenin genes vtgAo1 and vtgC during ovarian development in Chinese hook snout carp Opsariichthys bidens.
Vitellogenesis is essential for oocyte maturation. Vitellogenin (Vtg), a yolk precursor protein, plays an important role in oogenesis and vitellogenesis. Chinese hook snout carp Opsariichthys bidens is an economically important freshwater fish in China whose reproductive and developmental biology are not well understood. In this study, we undertook histological analysis to examine ovary development and oogenesis in O. bidens. The ovaries were divided into Stages II-V and oocytes were divided into perinuclear oocytes, cortical alveoli oocytes, vitellogenic oocytes and mature oocytes. Full-length cDNA sequences were cloned of two vtg genes from the liver of O. bidens, namely Ob-vtgAo1 and Ob-vtgC. Ob-vtgAo1 and Ob-vtgC cDNA are made up of 4136 and 4392 bases respectively and encode proteins containing 1335 and 1250 amino acids respectively. Ob-vtgAo1 contains three yolk protein domains: lipovitellin heavy chain (LvH), phosvitin (Pv) and lipovitellin light chain (LvL), whereas Ob-VtgC contains LvH and LvL, which are incomplete Vtgs. Ob-vtgAo1 and Ob-vtgC mRNA expression was significantly higher in the liver of O. bidens than in all other tissues. In oocytes of Stage II-III ovaries, yolk granules are almost absent and ovarian and hepatic Ob-vtgAo1 and Ob-vtgC expression is low. At Stage IV, the oocyte is filled with yolk granules and ovarian and hepatic Ob-vtgAo1 and Ob-vtgC expression is significantly increased. Collectively, these findings help us better understand vitellogenesis in O. bidens.
Topics: Animals; Carps; Cloning, Molecular; Female; Fish Proteins; Gene Expression Regulation, Developmental; Liver; Oocytes; Oogenesis; Ovary; Phylogeny; Real-Time Polymerase Chain Reaction; Reverse Transcriptase Polymerase Chain Reaction; Up-Regulation; Vitellogenesis; Vitellogenins
PubMed: 33858563
DOI: 10.1071/RD20294 -
Aquatic Toxicology (Amsterdam,... Jun 2022Octyl methoxycinnamate (OMC) is a common UV filter found in personal care products such as sunscreen and cosmetics. However, OMC's presence in wastewater has raised...
Octyl methoxycinnamate (OMC) is a common UV filter found in personal care products such as sunscreen and cosmetics. However, OMC's presence in wastewater has raised concerns that it could potentially pollute aquatic ecosystems because of its limited biodegradability and its estrogenic disrupting properties. In this study, we investigated the environmental toxicity of OMC and its potential biomarkers using the nematode Caenorhabditis elegans. Our results showed that body length, eggs in utero, and total brood size decreased with increasing dose (experimental concentrations = 0, 1, 5, 10, 100, 500 μM for body length and eggs in utero, and 0, 5, 10 μM for total brood size) in C. elegans after L1 larval stage (the first larval stage for 0 - 12 hours post-hatching) larval stage exposure to OMC. The minimum effective concentrations were 1, 5, and 10 μM, respectively. Modeling results demonstrated that the threshold concentration of OMC inducing 10% inhibited eggs in utero was 0.33 μM (95.11 μg/L). Furthermore, germline apoptosis was induced in 10 μM OMC-treated worms (experimental concentrations = 0, 5, 10 μM). Decreased mRNA levels of vitellogenin-related genes (vit-2 and vit-6) and increased mRNA levels of apoptosis-related genes (egl-1 and ced-3) were observed in 10 μM OMC-treated C. elegans (experimental concentrations = 0, 10 μM), suggesting that reproductive toxicity was associated with decreased vitellogenin levels and germline apoptosis. In summary, our study shows that OMC is reproductively toxic and leads to reduced egg formation and decreased brood size in C. elegans by reducing vitellogenin levels and promoting germline apoptosis.
Topics: Animals; Apoptosis; Caenorhabditis elegans; Ecosystem; Germ Cells; RNA, Messenger; Vitellogenins; Water Pollutants, Chemical
PubMed: 35397382
DOI: 10.1016/j.aquatox.2022.106149 -
Frontiers in Endocrinology 2021This review summarizes the bulk of evidence about the effect of glyphosate, both technical and formulated, on the ovarian maturation of female crabs, as well as the... (Review)
Review
This review summarizes the bulk of evidence about the effect of glyphosate, both technical and formulated, on the ovarian maturation of female crabs, as well as the effects of glyphosate on sperm production in males of the same species. After long-term assays, made during the 3-month pre-reproductive period of this species, both formulated and technical glyphosate were able to produce a significant incidence of oocyte reabsorption in the ovary, together with a concomitant decreased of vitellogenin content, at concentrations ranging from 0.2 to 1 mg/L. Despite this, after 32-day assays, glyphosate stimulated oocyte growth, in terms of a higher percentage of vitellogenic oocytes, suggesting that glyphosate could be acting as an endocrine disruptor. assays made with isolated ovarian pieces showed a decrease of vitellogenin content, in correlation with lower protein synthesis, although some advance in maturation was observed in the histological analysis. In male crabs exposed to both technical and formulated glyphosate at 1 mg/L, several reproductive imbalances were noted, such as a significant decrease of the sperm count, abnormal spermatophores, and possible disrupting effects of glyphosate on the androgenic gland.
Topics: Androgens; Animals; Brachyura; Dose-Response Relationship, Drug; Endocrine Disruptors; Female; Glycine; Herbicides; Male; Models, Animal; Oocytes; Ovary; Reproduction; Spermatozoa; Vitellogenins; Water Pollutants, Chemical; Glyphosate
PubMed: 33841335
DOI: 10.3389/fendo.2021.643168 -
Journal of Insect Science (Online) Mar 2018Vitellogenin (Vg) and vitellogenin receptor (VgR) play important roles in the vitellogenesis of insects. In this study, we cloned and characterized the two corresponding...
Vitellogenin (Vg) and vitellogenin receptor (VgR) play important roles in the vitellogenesis of insects. In this study, we cloned and characterized the two corresponding genes (TpVg and TpVgR) in an economically important insect, Thitarodes pui (Lepidoptera: Hepialidae), from the Tibetan plateau. The full length of TpVg is 5566 bp with a 5373 bp open reading frame (ORF) encoding 1,790 amino acids. Sequence alignment revealed that TpVg has three conserved domains: a Vitellogenin_N domain, a DUF1943 domain, and a von Willebrand factor type D domain (VWD). The full length of TpVgR is 5732 bp, with a 5397 bp ORF encoding 1798 amino acids. BLASTP showed that TpVgR belongs to the low-density lipoprotein receptor (LDLR) gene superfamily. Structural analysis revealed that TpVgR has a group of four structural domains: a ligand-binding domain (LBD), an epidermal growth factor (EGF)-precursor homology domain, a transmembrane (TM) domain, and a cytoplasmic domain. In addition, TpVgR has four cysteine-rich LDL repeats in the first ligand-binding site and seven in the second. Quantitative real-time polymerase chain reaction analysis revealed that the expression levels of TpVg and TpVgR are much higher in later pupa than in either the larval or adult stage, implying that the synthesis and uptake of Vg in T. pui occurs in the later pupal stage. These results will help us to understand the molecular mechanism of the reproductive capacity and will provide new insight into the mass rearing and utilization of T. pui.
Topics: Animals; Egg Proteins; Female; Moths; Phylogeny; Receptors, Cell Surface; Sequence Analysis, DNA; Vitellogenins
PubMed: 29718485
DOI: 10.1093/jisesa/iey010 -
Ecotoxicology and Environmental Safety Nov 2023Vitellogenin (VTG), a biomarker for endocrine activity, is a mechanistic component of the regulatory assessment of potential endocrine-disrupting properties of... (Review)
Review
Vitellogenin (VTG), a biomarker for endocrine activity, is a mechanistic component of the regulatory assessment of potential endocrine-disrupting properties of chemicals. This review of VTG data is based on changes reported for 106 substances in standard fish species. High intra-study and inter-laboratory variability in VTG concentrations was confirmed, as well as discrepancies in interpretation of results based on large differences between fish in the dilution water versus solvent control, or due to the presence of outlier measurements. VTG responses in fish were ranked against predictions for estrogen receptor agonist activity and aromatase inhibition from bioactivity model output and ToxCast in vitro assay results, respectively. These endocrine mechanisms explained most of the VTG responses in the absence of systemic toxicity, the magnitude of the VTG response being proportional to the in vitro potency. Interpretation of the VTG data was sometimes confounded by an alternative endocrine mechanism of action. There was evidence for both false positive and negative responses for VTG synthesis, but overall, it was rare for substances without endocrine activity in vitro to cause a concentration-dependent VTG response in fish in the absence of systemic toxicity. To increase confidence in the VTG results, we recommend improvements in the VTG measurement methodologies and greater transparency in reporting of VTG data (including quality control criteria for assay performance). This review supports the application of New Approach Methodologies (NAMs) by demonstrating that endocrine activity in vitro from mammalian cell lines is predictive for in vivo VTG response in fish, suggesting that in vitro mechanistic data could be used more broadly in decision-making to help reduce animal testing.
Topics: Animals; Vitellogenins; Fishes; Estrogens; Endocrine Disruptors; Water Pollutants, Chemical; Mammals
PubMed: 37827093
DOI: 10.1016/j.ecoenv.2023.115563 -
Science China. Life Sciences Mar 2023The maintenance of proteostasis is essential for cellular and organism healthspan. How proteostasis collapse influences reproductive span remains largely unclear. In...
The maintenance of proteostasis is essential for cellular and organism healthspan. How proteostasis collapse influences reproductive span remains largely unclear. In Caenorhabditis elegans, excess accumulation of vitellogenins, the major components in yolk proteins, is crucial for the development of the embryo and occurs throughout the whole body during the aging process. Here, we show that vitellogenin accumulation leads to reproduction cessation. Excess vitellogenin is accumulated in the intestine and transported into the germline, impairing lysosomal activity in these tissues. The lysosomal function in the germline is required for reproductive span by maintaining oocyte quality. In contrast, autophagy and sperm depletion are not involved in vitellogenin accumulation-induced reproductive aging. Our findings provide insights into how proteome imbalance has an impact on reproductive aging and imply that improvement of lysosomal function is an effective approach for mid-life intervention for maintaining reproductive health in mammals.
Topics: Animals; Male; Vitellogenins; Semen; Aging; Caenorhabditis elegans; Caenorhabditis elegans Proteins; Lysosomes; Mammals
PubMed: 36680676
DOI: 10.1007/s11427-022-2242-8 -
Biochimica Et Biophysica Acta.... Apr 2023Vitellogenins (Vtgs) are essential for female reproduction in oviparous animals, yet the exact roles and mechanisms remain unknown. In the present study, we knocked out...
Vitellogenins (Vtgs) are essential for female reproduction in oviparous animals, yet the exact roles and mechanisms remain unknown. In the present study, we knocked out vtg1, which is the most abundant Vtg in zebrafish, Danio rerio via the CRISPR/Cas 9 technology. We aimed to identify the roles of Vtg1 and related mechanisms in reproduction and development. We found that, the Vtg1-deficient female zebrafish reduced gonadosomatic index, egg production, yolk granules and mature follicles in ovary compared to the wide type (WT). Moreover, the Vtg1-deficient zebrafish diminished hatching rates, cumulative survival rate, swimming capacity and food intake, but increased malformation rate, and delayed swim bladder development during embryo and early-larval phases. The Vtg1-deficiency in female broodstock inhibited docosahexaenoic acid-enriched phosphatidylcholine (DHA-PC) transportation from liver to ovary, which lowered DHA-PC content in ovary and offspring during larval stage. However, the Vtg1-deficient zebrafish increased gradually the total DHA-PC content via exogeneous food intake, and the differences in swimming capacity and food intake returned to normal as they matured. Furthermore, supplementing Vtg1-deficient zebrafish with dietary PC and DHA partly ameliorated the impaired female reproductive capacity and larval development during early phases. This study indicates that, DHA and PC carried by Vtg1 are crucial for female fecundity, and affect embryo and larval development through maternal-nutrition effects. This is the first study elucidating the nutrient and physiological functions of Vtg1 and the underlying biochemical mechanisms in fish reproduction and development.
Topics: Animals; Female; Ovary; Zebrafish; Vitellogenins; Docosahexaenoic Acids; Liver; Reproduction; Lecithins
PubMed: 36708962
DOI: 10.1016/j.bbalip.2023.159289 -
Poultry Science May 2022Dietary supplementation of green tea powder (GTP) changes egg quality of hens, however, whether these changes affect incubation is still unknown. This study was to...
Dietary supplementation of green tea powder (GTP) changes egg quality of hens, however, whether these changes affect incubation is still unknown. This study was to compare the proteomic difference of incubated eggs from hens with GTP supplemented or not. Huainan partridge chickens (1,080) at 35 wk of age were allocated into 2 groups, one group fed basal diet (CG) and one group fed basal diet plus 1% GTP (EG). After 4 wk feeding, artificially fertilized eggs were collected for yolk cholesterol determination and incubation. During incubation, 6 embryos from each group were randomly selected in each day for yolk protein extraction and quantification. Yolk cholesterol content was significantly lower, while the hatchability was significantly higher in EG than that of the CG group (P < 0.05). Yolk protein concentration at embryonic days (ED) of 0, 2, 6, and 13 showed significant changes and were selected for proteomic analysis by 2-dimensional gel electrophoresis combined with liquid chromatography-tandem mass spectrometry. Fifty-one differentially expressed (DE) protein spots were identified among different incubation stages between CG and EG group which were mainly classified into vitellogenin, immunoglobulin, and ovoinhibitor, and occupied 45.1, 23.5, and 15.7%, respectively, to the total DE proteins. Ovotransferrin, participated in extracellular sequestering of iron ion process, was significantly lower in EG group than that of the CG group (P < 0.05). Ig light chain precursor (Immunoglobulin) exhibited higher expression at ED6 in EG group as compared with that of the CG group, and was participated in immune response related processes. Ovoinhibitor, mainly involved in protease binding activity, showed lower abundance at ED13 in EG group as compared with that of the CG group. Vitellogenin-3, showed lower expression in EG group as compared with that of the CG group, was mainly participated in lipid transportation and localization according to GO enrichment. Chickens fed diet with GTP provided eggs more antioxidant ability that increased hatchability, indicated that GTP could be considered as additive in breeding layer.
Topics: Animal Feed; Animals; Antioxidants; Chickens; Cholesterol; Diet; Egg Proteins; Egg Yolk; Female; Guanosine Triphosphate; Powders; Proteomics; Tea; Vitellogenins
PubMed: 35325830
DOI: 10.1016/j.psj.2022.101804