-
Iranian Journal of Parasitology 2017is an opportunistic amphizoic protozoan found in different fresh water sources. The aim of this study was to identify and characterize isolates from surface resting...
BACKGROUND
is an opportunistic amphizoic protozoan found in different fresh water sources. The aim of this study was to identify and characterize isolates from surface resting waters, in Northwest Iran.
METHODS
Samples were collected from twenty-two different areas, between May and Sep 2014. After filtration, samples were cultivated on non-nutrient agar. The extracted DNAs were amplified and sequenced using partial 18S rRNA in order to genotype and phylogenetic analyses.
RESULTS
Thirty-four (68%) out of 50 collected samples were positive for free-living amoebae based on both culture and morphological characterizations but 28 samples were identified as spp. by PCR. Sequentially, one isolate was identified as , (T5) (AN: KP940443, identity 99.7%-100%, and divergence 0.3%) whilst other sequenced isolates identified spp. (AN: KP940444-45) as very similar to and with identity 100% and divergence 0%.
CONCLUSION
Surface resting waters in Northwest Iran, were potentially contaminated with pathogenic amphizoic protozoan. Further studies will be required to determine other species and genotypes in the region.
PubMed: 28979345
DOI: No ID Found -
Scientific Reports Jul 2020Multinuclearity is a widespread phenomenon across the living world, yet how it is achieved, and the potential related advantages, are not systematically understood. In...
Multinuclearity is a widespread phenomenon across the living world, yet how it is achieved, and the potential related advantages, are not systematically understood. In this study, we investigate multinuclearity in amoebae. We observe that non-adherent amoebae are giant multinucleate cells compared to adherent ones. The cells solve their multinuclearity by a stretchy cytokinesis process with cytosolic bridge formation when adherence resumes. After initial adhesion to a new substrate, the progeny of the multinucleate cells is more numerous than the sibling cells generated from uninucleate amoebae. Hence, multinucleate amoebae show an advantage for population growth when the number of cells is quantified over time. Multiple nuclei per cell are observed in different amoeba species, and the lack of adhesion induces multinuclearity in diverse protists such as Acanthamoeba castellanii, Vermamoeba vermiformis, Naegleria gruberi and Hartmannella rhysodes. In this study, we observe that agitation induces a cytokinesis delay, which promotes multinuclearity. Hence, we propose the hypothesis that multinuclearity represents a physiological adaptation under non-adherent conditions that can lead to biologically relevant advantages.
Topics: Acanthamoeba castellanii; Cell Culture Techniques; Cell Nucleus; Cytokinesis; Microscopy, Electron, Scanning
PubMed: 32694508
DOI: 10.1038/s41598-020-68694-9