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Viruses Dec 2022Bacterial viruses known as bacteriophages have been demonstrated to be effective in killing foodborne pathogens such as . Adsorption is the first step in the phage-host...
Bacterial viruses known as bacteriophages have been demonstrated to be effective in killing foodborne pathogens such as . Adsorption is the first step in the phage-host interaction. In the present work, 10 phages were used to characterize the adsorption process on ATCC12022 in several physicochemical conditions related to food and in a food matrix. One-step growth curves were drawn for all the -phages evaluated. Furthermore, the adsorption rate for each of the 10 phages was determined. In addition, the influence of temperature, Na, Mg, pH, sucrose and glycerol on phage adsorption was investigated. Two phages (Shi22 and Shi30) showed higher burst sizes values (67 and 64 PFU cell, respectively) and burst times of 25 min to 30 min, while the other eight phages exhibited burst sizes ranging from 14 to 17 PFU cell with slower burst times. Furthermore, most phages achieved a high adsorption rate, and the adsorption constants () ranged from ~10 to 10 mL min. Regarding the influence of temperature, cations and pH, a high or moderate percentage of adsorption was observed for most of the phages evaluated. The adsorption decreased at increasing concentrations of Na, sucrose and glycerol, although at different levels, since adsorption was more affected by sucrose than by glycerol and Na for most phages. The adsorption obtained in Triptein soy broth (TSB) for most of the phages/strain systems evaluated was moderate or high, as well as those observed in a food matrix. Thus, our phages could potentially be used to improve food safety under a wide range of environmental conditions against foodborne pathogens.
Topics: Shigella flexneri; Bacteriophages; Adsorption; Glycerol; Shigella; Sucrose
PubMed: 36560819
DOI: 10.3390/v14122815 -
International Journal of Molecular... Aug 2023The influence of nanoscale surface topography on protein adsorption is highly important for numerous applications in medicine and technology. Herein, ferritin adsorption...
The influence of nanoscale surface topography on protein adsorption is highly important for numerous applications in medicine and technology. Herein, ferritin adsorption at flat and nanofaceted, single-crystalline AlO surfaces is investigated using atomic force microscopy and X-ray photoelectron spectroscopy. The nanofaceted surfaces are generated by the thermal annealing of AlO wafers at temperatures above 1000 °C, which leads to the formation of faceted saw-tooth-like surface topographies with periodicities of about 160 nm and amplitudes of about 15 nm. Ferritin adsorption at these nanofaceted surfaces is notably suppressed compared to the flat surface at a concentration of 10 mg/mL, which is attributed to lower adsorption affinities of the newly formed facets. Consequently, adsorption is restricted mostly to the pattern grooves, where the proteins can maximize their contact area with the surface. However, this effect depends on the protein concentration, with an inverse trend being observed at 30 mg/mL. Furthermore, different ferritin adsorption behavior is observed at topographically similar nanofacet patterns fabricated at different annealing temperatures and attributed to different step and kink densities. These results demonstrate that while protein adsorption at solid surfaces can be notably affected by nanofacet patterns, fine-tuning protein adsorption in this way requires the precise control of facet properties.
Topics: Ferritins; Adsorption; Medicine; Microscopy, Atomic Force; Photoelectron Spectroscopy
PubMed: 37628990
DOI: 10.3390/ijms241612808 -
Chemical Communications (Cambridge,... Mar 2022Metal-organic cages are macrocyclic structures that can possess an intrinsic void that can hold molecules for encapsulation, adsorption, sensing, and catalysis... (Review)
Review
Metal-organic cages are macrocyclic structures that can possess an intrinsic void that can hold molecules for encapsulation, adsorption, sensing, and catalysis applications. As metal-organic cages may be comprised from nearly any combination of organic and metal-containing components, cages can form with diverse shapes and sizes, allowing for tuning toward targeted properties. Therefore, their near-infinite design space is almost impossible to explore through experimentation alone and computational design can play a crucial role in exploring new systems. Although high-throughput computational design and screening workflows have long been known as powerful tools in drug and materials discovery, their application in exploring metal-organic cages is more recent. We show examples of structure prediction and host-guest/catalytic property evaluation of metal-organic cages. These examples are facilitated by advances in methods that handle metal-containing systems with improved accuracy and are the beginning of the development of automated cage design workflows. We finally outline a scope for how high-throughput computational methods can assist and drive experimental decisions as the field pushes toward functional and complex metal-organic cages. In particular, we highlight the importance of considering realistic, flexible systems.
Topics: Adsorption; Catalysis; Metals
PubMed: 35229861
DOI: 10.1039/d2cc00532h -
Analytical Sciences : the International... 2021A nanoporous material has been applied for the development of functional nanobiomaterials by utilizing its uniform pore structure and large adsorption capacity. The... (Review)
Review
A nanoporous material has been applied for the development of functional nanobiomaterials by utilizing its uniform pore structure and large adsorption capacity. The structure and stability of biomacromolecules, such as peptide, oligonucleotide, and protein, are primary factors to govern the performance of nanobiomaterials, so that their direct characterization methodologies are in progress. In this review, we focus on recent topics in the structural characterization of protein molecules adsorbed at a nanoporous material with uniform meso-sized pores. The thermal stabilities of the adsorbed proteins are also summarized to discuss whether the structure of the adsorbed protein molecules can be stabilized or not.
Topics: Adsorption; Nanopores; Proteins; Surface Properties
PubMed: 33431779
DOI: 10.2116/analsci.20SAR05 -
International Journal of Molecular... Mar 2023The application of the adsorption method in sewage treatment has recently become a hot spot. A novel magnetic clay-biochar composite (BNT-MBC) was fabricated by...
The application of the adsorption method in sewage treatment has recently become a hot spot. A novel magnetic clay-biochar composite (BNT-MBC) was fabricated by co-pyrolysis of bentonite and biomass after being impregnated with Fe (NO)·9HO. Its adsorption capacity for Cd(II) and methyl orange was approximately doubled, reaching a maximum of 26.22 and 63.34 mg/g, and could be easily separated from the solution by using external magnets with its saturation magnetization of 9.71 emu/g. A series of characterizations including surface morphology and pore structure, elemental analysis, functional group analysis and graphitization were carried out, showing that the specific surface area was increased 50 times by loading 20 wt.% bentonite, while its graphitization and oxygen-containing functional groups were also enhanced. The isotherm fitting indicated that Cd(II) was adsorbed in multiple layers, while methyl orange was in both monolayer and multilayer adsorptions. The kinetic fitting indicated that chemisorption was the rate-limiting step of both, and it was also a complex process controlled by two steps with the fitting of intra-particle diffusion. In the binary system of Cd(II) and methyl orange, the co-existing pollutants facilitated the adsorption of the original one, and there was no competition between adsorption sites of Cd(II) and methyl orange. BNT-MBC also exhibited good reusability and can be magnetically recovered for recycling. Thus, the magnetic clay-biochar composite BNT-MBC is a cost-effective and promising adsorbent for simultaneous removing Cd(II) and methyl orange from wastewater.
Topics: Adsorption; Cadmium; Clay; Bentonite; Charcoal; Water; Magnetic Phenomena; Water Pollutants, Chemical; Kinetics
PubMed: 36982828
DOI: 10.3390/ijms24065755 -
Biosensors Nov 2022Observing interfacial molecular adsorption and desorption dynamics in a label-free manner is fundamentally important for understanding spatiotemporal transports of...
Observing interfacial molecular adsorption and desorption dynamics in a label-free manner is fundamentally important for understanding spatiotemporal transports of matter and energy across interfaces. Here, we report a label-free real-time sensing technique utilizing strong optical second harmonic generation of monolayer 2D semiconductors. BSA molecule adsorption and desorption dynamics on the surface of monolayer MoS in liquid environments have been all-optically observed through time-resolved second harmonic generation (SHG) measurements. The proposed SHG detection scheme is not only interface specific but also expected to be widely applicable, which, in principle, undertakes a nanometer-scale spatial resolution across interfaces.
Topics: Adsorption; Second Harmonic Generation Microscopy
PubMed: 36421166
DOI: 10.3390/bios12111048 -
Molecules (Basel, Switzerland) Oct 2022The need for fresh and conveniently treated water has become a major concern in recent years. Molybdenum disulfide (MoS) nanomaterials are attracting attention in... (Review)
Review
The need for fresh and conveniently treated water has become a major concern in recent years. Molybdenum disulfide (MoS) nanomaterials are attracting attention in various fields, such as energy, hydrogen production, and water decontamination. This review provides an overview of the recent developments in MoS-based nanomaterials for water treatment via adsorption and photodegradation. Primary attention is given to the structure, properties, and major methods for the synthesis and modification of MoS, aiming for efficient water-contaminant removal. The combination of MoS with other components results in nanocomposites that can be separated easily or that present enhanced adsorptive and photocatalytic properties. The performance of these materials in the adsorption of heavy metal ions and organic contaminants, such as dyes and drugs, is reviewed. The review also summarizes current progress in the photocatalytic degradation of various water pollutants, using MoS-based nanomaterials under UV-VIS light irradiation. MoS-based materials showed good activity after several reuse cycles and in real water scenarios. Regarding the ecotoxicity of the MoS, the number of studies is still limited, and more work is needed to effectively evaluate the risks of using this nanomaterial in water treatment.
Topics: Photolysis; Adsorption; Molybdenum; Water Pollutants; Water Pollutants, Chemical; Nanocomposites; Metals, Heavy; Coloring Agents; Hydrogen
PubMed: 36296375
DOI: 10.3390/molecules27206782 -
International Journal of Environmental... Dec 2022In our work, the transition-metal-oxide precursor (TMO@BC, M = Fe, Co, Ni) has been loaded on the pollen carbon by the hydrothermal method and annealed at different...
In our work, the transition-metal-oxide precursor (TMO@BC, M = Fe, Co, Ni) has been loaded on the pollen carbon by the hydrothermal method and annealed at different temperatures to generate a composite material of metal oxide and pollen carbon in this study, which can effectively prevent agglomeration caused by a small size and magnetism. The XRD patterns of the samples showed that the as-synthesized metal oxides were γ-FeO, CoO, and NiO. In the 20 mg/L methyl orange adsorption experiment, the adsorption amount of CoO@C at 500 ℃ reached 19.32 mg/g and the removal rate was 96.61%. Therefore, CoO@C was selected for the adsorption correlation-model-fitting analysis, which was in line with the secondary reaction. The pseudo-second-order kinetic model (: 0.9683-0.9964), the intraparticle diffusion model, and the Freundlich adsorption isotherm model indicated that the adsorption process was the result of both physical and chemical adsorptions, and the judgment was based on the electrostatic action. The adsorption and removal efficiency of ciprofloxacin (CIP) by changing the pH of the reaction was about 80%, so the electrostatic attraction worked, but not the main factor. Recovered by an external magnetic field, the three-time recycling efficiency was still maintained at more than 80%. This novel biomass-derived magnetic porous carbon material embedded with transition-metal-oxide nanoparticles is highly promising for many applications, especially in the field of environmental remediation.
Topics: Oxides; Carbon; Porosity; Metals; Adsorption; Metal Nanoparticles; Magnetic Phenomena; Water Pollutants, Chemical; Kinetics; Charcoal
PubMed: 36554621
DOI: 10.3390/ijerph192416740 -
Molecules (Basel, Switzerland) Mar 2023Corrosion is the process of damaging materials, and corrosion of metallic materials frequently results in serious consequences. The addition of corrosion inhibitors is... (Review)
Review
Corrosion is the process of damaging materials, and corrosion of metallic materials frequently results in serious consequences. The addition of corrosion inhibitors is the most effective means of preventing metal corrosion. Until now, researchers have made unremitting efforts in the research of high-efficiency green corrosion inhibitors, and research on biomass corrosion inhibitors in a class of environmentally friendly corrosion inhibitors is currently quite promising. This work presents the classification of green biomass corrosion inhibitors in detail, including plant-based corrosion inhibitors, amino acid corrosion inhibitors, and biosurfactant corrosion inhibitors, based on the advantages of easy preparation, environmental friendliness, high corrosion inhibition efficiency, and a wide application range of biomass corrosion inhibitors. This work also introduces the preparation methods of biomass corrosion inhibitors, including hydrolysis, enzymatic digestion, the heating reflux method, and microwave extraction. In addition, the corrosion inhibition mechanisms of green biomass corrosion inhibitors, including physical adsorption, chemisorption, and film-forming adsorption, and evaluation methods of biomass corrosion inhibitors are also explicitly described. This study provides valuable insights into the development of green corrosion inhibitors.
Topics: Corrosion; Biomass; Metals; Adsorption
PubMed: 36985804
DOI: 10.3390/molecules28062832 -
Revista de Investigacion Clinica;... Dec 2023Membranes and sorbents play a crucial role in extracorporeal blood purification therapies, which aim to remove harmful molecules and toxins from the blood. Over the... (Review)
Review
Membranes and sorbents play a crucial role in extracorporeal blood purification therapies, which aim to remove harmful molecules and toxins from the blood. Over the years, advancements in hemodialysis (HD) membranes and sorbents have significantly enhanced their safety and effectiveness. This review article will summarize the latest breakthroughs in the development and clinical application of HD membranes and sorbents. We will commence with a concise examination of the mechanisms involved in solute transport across membranes and sorbents. Subsequently, we will explore the evolutionary path of HD membranes, from early cellophane membranes to high-flux membranes, including the development of high-cutoff membranes and the emergence of medium- cutoff membranes. We will discuss each type of HD membrane's advantages and limitations, highlighting the most promising advancements in novel biomaterials and biocompatibility, technologies, research in membrane performance, and their clinical applications. Furthermore, we will delve into the evolution and progress of sorbent technology, tracing its historical development, outlining its key characteristics, examining the mechanism involved in the adsorption process, and exploring its clinical application. This review aims to underscore the growth and future landscape of HD membranes and sorbents in extracorporeal blood purification techniques.
Topics: Humans; Renal Dialysis; Adsorption
PubMed: 37913784
DOI: 10.24875/RIC.23000223