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Scientific Reports Feb 2020Biofluorescence is the absorption of electromagnetic radiation (light) at one wavelength followed by its reemission at a lower energy and longer wavelength by a living...
Biofluorescence is the absorption of electromagnetic radiation (light) at one wavelength followed by its reemission at a lower energy and longer wavelength by a living organism. Previous studies have documented the widespread presence of biofluorescence in some animals, including cnidarians, arthropods, and cartilaginous and ray-finned fishes. Many studies on biofluorescence have focused on marine animals (cnidarians, cartilaginous and ray-finned fishes) but we know comparatively little about the presence of biofluorescence in tetrapods. We show for the first time that biofluorescence is widespread across Amphibia, with a focus on salamanders (Caudata), which are a diverse group with a primarily Holarctic distribution. We find that biofluorescence is not restricted to any particular family of salamanders, there is striking variation in their fluorescent patterning, and the primary wavelengths emitted in response to blue excitation light are within the spectrum of green light. Widespread biofluorescence across the amphibian radiation is a previously undocumented phenomenon that could have significant ramifications for the ecology and evolution of these diverse and declining vertebrates. Our results provide a roadmap for future studies on the characterization of molecular mechanisms of biofluorescence in amphibians, as well as directions for investigations into the potential impact of biofluorescence on the visual ecology and behavior of biofluorescent amphibians.
Topics: Amphibians; Animals; Ecology; Fluorescence; Phylogeny; Pigmentation; Urodela
PubMed: 32108141
DOI: 10.1038/s41598-020-59528-9 -
Humane acute testing with tadpoles for risk assessment of chemicals: Avoidance instead of lethality.Chemosphere Sep 2022In spite of the sensitivity of amphibians to contamination, data from fish have been commonly used to predict the effects of chemicals on aquatic life stages. However,... (Review)
Review
In spite of the sensitivity of amphibians to contamination, data from fish have been commonly used to predict the effects of chemicals on aquatic life stages. However, recent studies have highlighted that toxicity data derived from fish species may not protect all the aquatic life stages of amphibians. For pesticide toxicity assessment (PTA), EFSA has highlighted that more information on lethal toxicity for the aquatic life stages of amphibians is still needed to reduce uncertainties. The current review aims to propose a test with amphibians based on spatial avoidance, as a more humane alternative method to the lethality tests for chemicals. A review of lethal toxicity tests carried out with amphibians in the period between 2018 and 2021 is presented, then we discuss the suitability of using fish toxicity data as a surrogate to predict the effects on more sensitive amphibian groups. The possible differences in sensitivity to chemicals may justify the need to develop further tests with amphibian embryos and larvae in order to reduce uncertainties. A new test is proposed focused on the avoidance behaviour of organisms fleeing from contamination to replace lethal tests. As avoidance indicates the threshold at which organisms will flee from contamination, a reduction in the population density, or its disappearance, at the local scale due to emigration is expected, with ecological consequences analogous to mortality. Avoidance tests provide an ethical advantage over lethal tests as they respect the concepts of the 3 Rs (mainly Refinement), reducing the suffering of the organisms.
Topics: Amphibians; Animals; Fishes; Larva; Risk Assessment; Toxicity Tests; Water Pollutants, Chemical
PubMed: 35691390
DOI: 10.1016/j.chemosphere.2022.135197 -
Bio Systems Sep 2019In 1901 T.H. Morgan proposed in "Regeneration" that pattern formation in amphibian limb regeneration is a stepwise process. Since, biologist have continued to piece... (Review)
Review
In 1901 T.H. Morgan proposed in "Regeneration" that pattern formation in amphibian limb regeneration is a stepwise process. Since, biologist have continued to piece together the molecular components of this process to better understand the "patterning code" responsible for regenerate formation. Within this context, several different models have been proposed; however, all are based on one of two underlying hypotheses. The first is the "morphogen hypothesis" that dictates that pattern emerges from localized expression of signaling molecules, which produce differing position-specific cellular responses in receptive cells depending on the intensity of the signal. The second hypothesis is that cells in the remaining tissues retain memory of their patterning information, and use this information to generate new cells with the missing positional identities. A growing body of evidence supports the possibility that these two mechanisms are not mutually exclusive. Here, we propose our theory of hierarchical pattern formation, which consists of 4 basic steps. The first is the existence of cells with positional memory. The second is the communication of positional information through cell-cell interactions in a regeneration-permissive environment. The third step is the induction of molecular signaling centers. And the last step is the interpretation of these signals by specialized cell types to ultimately restore the limb in its entirety. Biological codes are intertwined throughout this model, and we will discuss their multiple roles and mechanisms.
Topics: Amphibians; Animals; Body Patterning; Caenorhabditis elegans; Cell Communication; Extremities; Fibroblasts; Humans; Models, Biological; Morphogenesis; Regeneration; Signal Transduction; Urodela
PubMed: 31295535
DOI: 10.1016/j.biosystems.2019.103989 -
Scientific Reports Nov 2023The major evolutionary transition from fish to amphibian included Late Devonian tetrapods that were neither fish nor amphibian. They had thick necks and small limbs with...
The major evolutionary transition from fish to amphibian included Late Devonian tetrapods that were neither fish nor amphibian. They had thick necks and small limbs with many digits on elongate flexuous bodies more suitable for water than land. Habitats of Devonian tetrapods are of interest in assessing selective pressures on their later evolution for land within three proposed habitats: 1, tidal flats, 2, desert ponds, and 3, woodland streams. Here we assess paleoenvironments of the Late Devonian tetrapod Sinostega from paleosols in Shixiagou Canyon near Zhongning, Ningxia, China. Fossil tetrapods, fish, molluscs, and plants of the Zhongning Formation are associated with different kinds of paleosols, representing early successional vegetation, seasonal wetlands, desert shrublands, and riparian woodlands, and paleoclimates ranging from semiarid moderately seasonal to monsoonal subhumid. The tetrapod Sinostega was found in a paleochannel of a meandering stream below a deep-calcic paleosol supporting well drained progymnosperm woodland in a monsoonal subhumid paleoclimate. This habitat is similar to that of the tetrapods Densignathus, Hynerpeton, and an indeterminate watcheeriid from Pennsylvania, USA. Chinese and Pennsylvanian Late Devonian tetrapods lived in productive woodland streams, choked with woody debris as a refuge from large predators. Habitats of other Devonian tetrapods have yet to be assessed from studies of associated paleosols as evidence for their ancient climate and vegetation.
Topics: Animals; Biological Evolution; Fossils; Fishes; Amphibians; Ecosystem
PubMed: 37990036
DOI: 10.1038/s41598-023-47728-y -
American Journal of Physiology. Cell... Oct 2022Professor Hans H. Ussing (1911-2000) was one of the founding members of the field of epithelial cell biology. He is most famous for the electrophysiological technique... (Review)
Review
Professor Hans H. Ussing (1911-2000) was one of the founding members of the field of epithelial cell biology. He is most famous for the electrophysiological technique that he developed to measure electrogenic ion flux across epithelial tissues. Ussing-style electrophysiology has been applied to multiple tissues and has informed fields as diverse as amphibian biology and medicine. In the latter, this technique has contributed to a basic understanding of maladies such as hypertension, polycystic kidney disease, cystic fibrosis, and diarrheal diseases to mention but a few. In addition to this valuable contribution to biological methods, Prof. Ussing also provided strong evidence for the concept of active transport several years before the elucidation of NaKATPase. In addition, he provided cell biologists with the important concept of polarized epithelia with specific and different transporters found in the apical and basolateral membranes, thus providing these cells with the ability to conduct directional, active and passive transepithelial transport. My studies have used Ussing chamber electrophysiology to study the toad urinary bladder, an amphibian cell line, renal cell lines, and, most recently, choroid plexus cell lines. This technique has formed the basis of our in vitro mechanistic studies that are used in an iterative manner with animal models to better understand disease progress and treatment. I was honored to be invited to deliver the 2022 Hans Ussing Lecture sponsored by the Epithelial Transport Group of the American Physiological Society. This manuscript is a version of the material presented in that lecture.
Topics: Adenosine Triphosphatases; Amphibians; Animals; Biological Transport; Brain; Epithelium; Kidney; Male; Mammals
PubMed: 36036449
DOI: 10.1152/ajpcell.00303.2022 -
PLoS Biology Jan 2022The trade-off between offspring size and number is central to life history strategies. Both the evolutionary gain of parental care or more favorable habitats for...
The trade-off between offspring size and number is central to life history strategies. Both the evolutionary gain of parental care or more favorable habitats for offspring development are predicted to result in fewer, larger offspring. However, despite much research, it remains unclear whether and how different forms of care and habitats drive the evolution of the trade-off. Using data for over 800 amphibian species, we demonstrate that, after controlling for allometry, amphibians with direct development and those that lay eggs in terrestrial environments have larger eggs and smaller clutches, while different care behaviors and adaptations vary in their effects on the trade-off. Specifically, among the 11 care forms we considered at the egg, tadpole and juvenile stage, egg brooding, male egg attendance, and female egg attendance increase egg size; female tadpole attendance and tadpole feeding decrease egg size, while egg brooding, tadpole feeding, male tadpole attendance, and male tadpole transport decrease clutch size. Unlike egg size that shows exceptionally high rates of phenotypic change in just 19 branches of the amphibian phylogeny, clutch size has evolved at exceptionally high rates in 135 branches, indicating episodes of strong selection; egg and tadpole environment, direct development, egg brooding, tadpole feeding, male tadpole attendance, and tadpole transport explain 80% of these events. By explicitly considering diversity in parental care and offspring habitat by stage of offspring development, this study demonstrates that more favorable conditions for offspring development promote the evolution of larger offspring in smaller broods and reveals that the diversity of parental care forms influences the trade-off in more nuanced ways than previously appreciated.
Topics: Amphibians; Animals; Biological Evolution; Body Size; Clutch Size; Ecosystem; Female; Life History Traits; Male; Maternal Behavior; Ovum; Paternal Behavior; Reproduction
PubMed: 34982764
DOI: 10.1371/journal.pbio.3001495 -
BMC Genomics Mar 2023Regeneration studies help to understand the strategies that replace a lost or damaged organ and provide insights into approaches followed in regenerative medicine and...
BACKGROUND
Regeneration studies help to understand the strategies that replace a lost or damaged organ and provide insights into approaches followed in regenerative medicine and engineering. Amphibians regenerate their limbs effortlessly and are indispensable models to study limb regeneration. Xenopus and axolotl are the key models for studying limb regeneration but recent studies on non-model amphibians have revealed species specific differences in regeneration mechanisms.
RESULTS
The present study describes the de novo transcriptome of intact limbs and three-day post-amputation blastemas of tadpoles and froglets of the Asian tree frog Polypedates maculatus, a non-model amphibian species commonly found in India. Differential gene expression analysis between early tadpole and froglet limb blastemas discovered species-specific novel regulators of limb regeneration. The present study reports upregulation of proteoglycans, such as epiphycan, chondroadherin, hyaluronan and proteoglycan link protein 1, collagens 2,5,6, 9 and 11, several tumour suppressors and methyltransferases in the P. maculatus tadpole blastemas. Differential gene expression analysis between tadpole and froglet limbs revealed that in addition to the expression of larval-specific haemoglobin and glycoproteins, an upregulation of cysteine and serine protease inhibitors and downregulation of serine proteases, antioxidants, collagenases and inflammatory genes in the tadpole limbs were essential for creating an environment that would support regeneration. Dermal myeloid cells were GAG+, EPYC+, INMT+, LEF1+ and SALL4+ and seemed to migrate from the unamputated regions of the tadpole limb to the blastema. On the other hand, the myeloid cells of the froglet limb blastemas were few and probably contributed to sustained inflammation resulting in healing.
CONCLUSIONS
Studies on non-model amphibians give insights into alternate tactics for limb regeneration which can help devise a plethora of methods in regenerative medicine and engineering.
Topics: Animals; Transcriptome; Regeneration; Xenopus laevis; Anura; Extremities; Gene Expression Profiling
PubMed: 36927452
DOI: 10.1186/s12864-023-09205-8 -
Stem Cell Research May 2021Mesenchymal stem cells (MSCs) are multipotent cells that exist in mesenchymal tissues such as bone marrow and are able to differentiate into osteocytes, chondrocytes,...
Mesenchymal stem cells (MSCs) are multipotent cells that exist in mesenchymal tissues such as bone marrow and are able to differentiate into osteocytes, chondrocytes, and adipocytes. MSCs are generally collected as adherent cells on a plastic dish, and are positive for markers such as CD44, CD73, CD90, CD105 and CD166, and negative for CD11b, CD14, CD19, CD31, CD34, CD45, CD79a and HLA-DR. MSCs have been established from many kinds of mammals, but MSCs from amphibians have not yet been reported. We cultured adherent cells from the bone marrow of Xenopus laevis by modifying the protocol for culturing mammalian MSCs. The morphology of these cells was similar to that of mammalian MSCs. The amphibian MSCs were positive for cd44, cd73, cd90 and cd166, and negative for cd11b, cd14, cd19, cd31, cd34, cd45, cd79a and hla-dra. Moreover, they could be induced to differentiate into osteocyte-, chondrocyte-, and adipocyte-lineage cells by cytokine induction systems that were similar to those used for mammalian MSC differentiation. Thus, they are considered to be similar to mammalian MSCs. Unlike mammals, amphibians have high regenerative capacity. The findings from the present study will allow for future research to reveal how Xenopus MSCs are involved in the amphibian regenerative capacity and to elucidate the differences in the regenerative capacity between mammals and amphibians.
Topics: Animals; Bone Marrow; Bone Marrow Cells; Cell Differentiation; Cells, Cultured; Mesenchymal Stem Cells; Xenopus laevis
PubMed: 33892293
DOI: 10.1016/j.scr.2021.102341 -
Global Change Biology Sep 2023Shifts between native and alien climatic niches pose a major challenge for predicting biological invasions. This is particularly true for insular species because...
Shifts between native and alien climatic niches pose a major challenge for predicting biological invasions. This is particularly true for insular species because geophysical barriers could constrain the realization of their fundamental niches, which may lead to underestimates of their invasion potential. To investigate this idea, we estimated the frequency of shifts between native and alien climatic niches and the magnitude of climatic mismatches using 80,148 alien occurrences of 46 endemic insular amphibian, reptile, and bird species. Then, we assessed the influence of nine potential predictors on climatic mismatches across taxa, based on species' characteristics, native range physical characteristics, and alien range properties. We found that climatic mismatch is common during invasions of endemic insular birds and reptiles: 78.3% and 55.1% of their respective alien records occurred outside of the environmental space of species' native climatic niche. In comparison, climatic mismatch was evident for only 16.2% of the amphibian invasions analyzed. Several predictors significantly explained climatic mismatch, and these varied among taxonomic groups. For amphibians, only native range size was associated with climatic mismatch. For reptiles, the magnitude of climatic mismatch was higher for species with narrow native altitudinal ranges, occurring in topographically complex or less remote islands, as well as for species with larger distances between their native and alien ranges. For birds, climatic mismatch was significantly larger for invasions on continents with higher phylogenetic diversity of the recipient community, and when the invader was more evolutionarily distinct. Our findings highlight that apparently common niche shifts of insular species may jeopardize our ability to forecast their potential invasions using correlative methods based on climatic variables. Also, we show which factors provide additional insights on the actual invasion potential of insular endemic amphibians, reptiles, and birds.
Topics: Animals; Introduced Species; Ecosystem; Phylogeny; Amphibians; Reptiles; Birds
PubMed: 37395619
DOI: 10.1111/gcb.16849 -
Scientific Reports Apr 2022Although 23% of Thailand's land is in protected areas, these are vulnerable to climate change. We used spatial distribution modelling for 866 vertebrate and 591 plant...
Although 23% of Thailand's land is in protected areas, these are vulnerable to climate change. We used spatial distribution modelling for 866 vertebrate and 591 plant species to understand potential climate change impacts on species in protected areas. Most mammals, birds, and plants were projected to decline by 2070, but most amphibians and reptiles were projected to increase. By 2070 under RCP8.5, 54% of modeled species will be threatened and 11 nationally extinct. However, SDMs are sensitive to truncation of the climate space currently occupied by habitat loss and hunting, and apparent truncation by data limitations. In Thailand, lowland forest clearance has biased records for forest-dependent species to cooler uplands (> 250 m a.s.l.) and hunting has confined larger vertebrates to well-protected areas. In contrast, available data is biased towards lowland non-forest taxa for amphibians and reptiles. Niche truncation may therefore have resulted in overestimation of vulnerability for some mammal and plant species, while data limitations have likely led to underestimation of the threat to forest-dependent amphibians and reptiles. In view of the certainty of climate change but the many uncertainties regarding biological responses, we recommend regular, long-term monitoring of species and communities to detect early signals of climate change impacts.
Topics: Amphibians; Animals; Biodiversity; Climate Change; Conservation of Natural Resources; Ecosystem; Mammals; Thailand
PubMed: 35383264
DOI: 10.1038/s41598-022-09767-9