-
The Korean Journal of Parasitology Aug 2015The present study was performed to report 15 anisakiasis cases in Korea and to review the Korean cases reported in the literature. Total 32 Anisakis type I larvae were...
The present study was performed to report 15 anisakiasis cases in Korea and to review the Korean cases reported in the literature. Total 32 Anisakis type I larvae were detected in the stomach of 15 patients by the endoscopy. Single worm was detected from 12 cases, and even 9 larvae were found from 2 cases. Epigastric pain was most commonly manifested in almost all cases, and hemoptysis and hematemesis were seen in 1 case each. Symptom manifestations began at 10-12 hr after eating fish in 73.3% cases. Endoscopy was performed 1-2 days after the symptom onset in most cases. The common conger, Conger myriaster, was the probable infection source in 7 cases. In the review of Korean anisakiasis cases, thus far, total 645 cases have been reported in 64 articles. Anisakis type I larva was the most frequently detected (81.3%). The favorable infection site of larvae was the stomach (82.4%). The common conger was the most probable source of human infections (38.6%). Among the total 404 cases which revealed the age and sex of patients, 185 (45.8%) were males, and the remaining 219 (54.2%) were female patients. The age prevalence was the highest in forties (34.7%). The seasonal prevalence was highest in winter (38.8%). By the present study, 15 cases of gastric anisakiasis are added as Korean cases, and some epidemiological characteristics of Korean anisakiasis were clarified.
Topics: Adult; Animals; Anisakiasis; Anisakis; Female; Fish Diseases; Fishes; Food Contamination; Humans; Larva; Male; Middle Aged; Prevalence; Republic of Korea; Stomach; Stomach Diseases
PubMed: 26323845
DOI: 10.3347/kjp.2015.53.4.465 -
Genes Mar 2020Ascaridoid nematodes are widespread in marine fishes. Despite their major socioeconomic importance, mechanisms associated to the fish-borne zoonotic disease anisakiasis...
Ascaridoid nematodes are widespread in marine fishes. Despite their major socioeconomic importance, mechanisms associated to the fish-borne zoonotic disease anisakiasis are still obscure. RNA-Seq and de-novo assembly were herein applied to RNA extracted from larvae and dissected pharynx of (HA), a non-pathogenic nematode. Assembled transcripts in HA were annotated and compared to the transcriptomes of the zoonotic species sensu stricto (AS) and (AP). Approximately 60,000,000 single-end reads were generated for HA, AS and AP. Transcripts in HA encoded for 30,254 putative peptides while AS and AP encoded for 20,574 and 20,840 putative peptides, respectively. Differential gene expression analyses yielded 471, 612 and 526 transcripts up regulated in the pharynx of HA, AS and AP. The transcriptomes of larvae and pharynx of HA were enriched in transcripts encoding collagen, peptidases, ribosomal proteins and in heat-shock motifs. Transcripts encoding proteolytic enzymes, anesthetics, inhibitors of primary hemostasis and virulence factors, anticoagulants and immunomodulatory peptides were up-regulated in AS and AP pharynx. This study represents the first transcriptomic characterization of a marine parasitic nematode commonly recovered in fish and probably of negligible concern for public health.
Topics: Animals; Anisakis; Conserved Sequence; Fishes; Genome, Helminth; Pharynx; Sequence Homology; Transcriptome; Virulence
PubMed: 32197414
DOI: 10.3390/genes11030321 -
Revista Espanola de Enfermedades... Sep 2015Intestinal anisakiasis is a rare parasitic disease and difficult to diagnose due to symptoms are not specific, so it is considered an underdiagnosed disease. The...
Intestinal anisakiasis is a rare parasitic disease and difficult to diagnose due to symptoms are not specific, so it is considered an underdiagnosed disease. The clinical suspicion with a correct diagnosis of anisakiasis allows the establishment of a correct treatment; in most cases, the resolution is possible with conservative treatment, avoiding unnecessary surgery to the preoperative differential diagnosis of acute abdomen. We report the case of apatient who required urgent surgery secondary to an exacerbation of chronic anisakiasis.
Topics: Anisakiasis; Chronic Disease; Humans; Ileal Neoplasms; Male; Mesentery; Middle Aged; Tomography, X-Ray Computed
PubMed: 26334466
DOI: No ID Found -
Internal Medicine (Tokyo, Japan) 2016
PubMed: 27980274
DOI: 10.2169/internalmedicine.55.7433 -
Cancer Diagnosis & Prognosis 2021Anisakiasis-related gastric eosinophilic granuloma is rare.
BACKGROUND
Anisakiasis-related gastric eosinophilic granuloma is rare.
CASE REPORT
Herein, we report a patient with anisakiasis-related gastric eosinophilic granuloma who was treated with laparoscopic and endoscopic cooperative surgery (LECS). A 59-year-old woman was presented to our hospital for further examination of a gastric lesion that was initially diagnosed by a local medical doctor. Esophagogastroduodenoscopy showed a submucosal tumor-like lesion in the lower body of the stomach. Endoscopic ultrasonography showed a heterogeneous hypoechoic submucosal mass lesion in the submucosal layer measuring 10 mm, without evidence of deep involvement. Under a clinical diagnosis of gastrointestinal stromal tumor, the patient underwent LECS. Gross appearance of the resected specimen revealed a 1.5×1.0 cm submucosal tumor-like lesion. Microscopic examination revealed necrosed insects consistent with the characteristics of gastric anisakiasis, around which prominent eosinophilic infiltration and granulomas were observed. This prompted a diagnosis of gastric eosinophilic granuloma related to anisakiasis.
CONCLUSION
To the best of our knowledge, this is the second case of gastric eosinophilic granuloma related to anisakiasis resected by LECS in the English medical literature. LECS might be a useful procedure for minimally invasive therapeutic diagnosis.
PubMed: 35403157
DOI: 10.21873/cdp.10068 -
Helminthologia Dec 2019The occurrence of zoonotic parasitic nematodes in Atlantic chub mackerels ( syn. ) from Libyan waters was investigated, using epizootiological estimations and molecular...
The occurrence of zoonotic parasitic nematodes in Atlantic chub mackerels ( syn. ) from Libyan waters was investigated, using epizootiological estimations and molecular specific characterization of larvae. Nematodes belonging to spp., the main etiological agent of anisakiasis in Mediterranean waters, and to spp. so far considered not pathogenic to humans, were detected. Prevalence values were generally high in visceral cavities (over 40 % for both parasites) while were low for (around 1 %) and null for in muscles. Moreover, the level of infections was associated with seasons, a feature potentially useful to plan fishing captures and to elaborate risk mitigation strategies for anisakiasis. Species molecular identification performed on a subsample described the presence of as the predominant species, along with and the hybrids ( and sensu stricto), thus posing a concrete zoonotic risk following the consumption of such fish species as a raw preparation.
PubMed: 31708677
DOI: 10.2478/helm-2019-0034 -
The American Journal of Tropical... Aug 2016Human anisakiasis is a zoonosis acquired by eating raw or undercooked infected seafood. Herein, we report a case of acute dysentery caused by anisakiasis in a...
Human anisakiasis is a zoonosis acquired by eating raw or undercooked infected seafood. Herein, we report a case of acute dysentery caused by anisakiasis in a 64-year-old man in Malaysia. A colonoscopy was performed and a nematode larva was found penetrating the mucosa of the ascending colon. Bleeding was observed at the site of penetration. Y-shaped lateral epidermal cords were seen from the cross section of the worm, which is a prominent feature of Anisakis larva. Molecular analysis using polymerase chain reaction of cytochrome oxidase 2 (cox2) gene confirmed the specimen to be larva of Anisakis simplex.
Topics: Animals; Anisakiasis; Anisakis; Colon; Colonoscopy; Dysentery; Humans; Larva; Malaysia; Male; Middle Aged; Seafood
PubMed: 27325803
DOI: 10.4269/ajtmh.16-0007 -
Internal Medicine (Tokyo, Japan) 2016
PubMed: 27580560
DOI: 10.2169/internalmedicine.55.6918 -
Comparative Proteomics Analysis of s.s.-Evaluation of the Response of Invasive Larvae to Ivermectin.Genes Jun 2020Ivermectin (IVM), an antiparasitic drug, has a positive effect against s.s. infection and has been used for the treatment and prevention of anisakiasis in humans....
Ivermectin (IVM), an antiparasitic drug, has a positive effect against s.s. infection and has been used for the treatment and prevention of anisakiasis in humans. However, the molecular mechanism of action of IVM on s.s. remains unknown. Herein, tandem mass tag (TMT) labeling and extensive liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) analysis were used to identify the effect of IVM on the proteome of s.s. . During the study, 3433 proteins, of which 1247 had at least two protein unique peptides, were identified. Comparative proteomics analysis revealed that 59 proteins were differentially regulated (DRPs) in IVM-treated larvae, of which 14 proteins were upregulated and 38 were downregulated after 12 h of culture, but after 24 h, 12 proteins were upregulated and 22 were downregulated. The transcription level of five randomly selected DRPs was determined by real-time PCR as a supplement to the proteomic data. The functional enrichment analysis showed that most of the DRPs were involved in oxidoreductase activity, immunogenicity, protein degradation, and other biological processes. This study has, for the first time, provided comprehensive proteomics data on s.s. response to IVM and might deliver new insight into the molecular mechanism by which IVM acts on invasive larvae of s.s.
Topics: Animals; Anisakiasis; Anisakis; Chromatography, Liquid; Gene Expression Regulation; Humans; Ivermectin; Larva; Proteome; Proteomics; Tandem Mass Spectrometry
PubMed: 32604878
DOI: 10.3390/genes11060710 -
Emerging Infectious Diseases Jun 2022Human Pseudoterranova decipiens larval infections were diagnosed by molecular analysis of mitochondrial cox1 and nd1 genes in 12 health check-up patients in South Korea...
Human Pseudoterranova decipiens larval infections were diagnosed by molecular analysis of mitochondrial cox1 and nd1 genes in 12 health check-up patients in South Korea during 2002-2020. Based on high genetic identity (99.3%-100% for cox1 and 96.7%-98.0% for nd1), we identified all 12 larvae as P. decipiens sensu stricto.
Topics: Animals; Anisakiasis; Anisakis; Ascaridoidea; Humans; Larva; Republic of Korea
PubMed: 35608950
DOI: 10.3201/eid2806.212483