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Frontiers in Plant Science 2019Efficient seed dispersal in flowering plants is enabled by the development of fruits, which can be either dehiscent or indehiscent. Dehiscent fruits open at maturity to...
Efficient seed dispersal in flowering plants is enabled by the development of fruits, which can be either dehiscent or indehiscent. Dehiscent fruits open at maturity to shatter the seeds, while indehiscent fruits do not open and the seeds are dispersed in various ways. The diversity in fruit morphology and seed shattering mechanisms is enormous within the flowering plants. How these different fruit types develop and which molecular networks are driving fruit diversification is still largely unknown, despite progress in eudicot model species. The orchid family, known for its astonishing floral diversity, displays a huge variation in fruit dehiscence types, which have been poorly investigated. We undertook a combined approach to understand fruit morphology and dehiscence in different orchid species to get more insight into the molecular network that underlies orchid fruit development. We describe fruit development in detail for the epiphytic orchid species and compare it to two terrestrial orchid species: and . Our anatomical analysis provides further evidence for the split carpel model, which explains the presence of three fertile and three sterile valves in most orchid species. Interesting differences were observed in the lignification patterns of the dehiscence zones. While and develop a lignified layer at the valve boundaries, fruits did not lignify at these boundaries, but formed a cuticle-like layer instead. We characterized orthologs of fruit-associated MADS-domain transcription factors and of the Arabidopsis dehiscence-related genes and in , and found that the key players of the eudicot fruit regulatory network appear well-conserved in monocots. Protein-protein interaction studies revealed that MADS-domain complexes comprised of FRUITFULL (FUL), SEPALLATA (SEP) and AGAMOUS (AG) /SHATTERPROOF (SHP) orthologs can also be formed in , and that the expression of , and can be associated with dehiscence zone development similar to Arabidopsis. Our expression analysis also indicates differences, however, which may underlie fruit divergence.
PubMed: 30838009
DOI: 10.3389/fpls.2019.00137 -
Ginekologia Polska 2020We wanted to identify risk factors for dehiscence of cesarean section (CS) scars in patients undergoing repeated cesarean section.
OBJECTIVE
We wanted to identify risk factors for dehiscence of cesarean section (CS) scars in patients undergoing repeated cesarean section.
MATERIAL AND METHODS
This was a retrospective case-control study over a 3-year period in our medical center (2011-2014), comparing women who had repeated CS without complications and women diagnosed with dehiscence. Data were collected from medical records and the groups were compared for demographic and obstetrical data.
RESULTS
Dehiscence was identified in 27 women, while 54 women without dehiscence were the control group. Statistically significant differences were found in the need for augmentation, the number of previous cesarean sections, cesarean section in the active phase of labor and length of hospitalization.
DISCUSSION
The need for augmentation of labor, CS in the nonactive stage and more than one cesarean section, all increased the risk of dehiscence. There was no association between dehiscence and scar pain, time elapsed since the previous cesarean section, the method of wound closure or fever.
Topics: Adult; Case-Control Studies; Cesarean Section, Repeat; Female; Humans; Israel; Medical Records; Pregnancy; Pregnancy Complications; Pregnancy Outcome; Prevalence; Retrospective Studies; Risk Factors; Surgical Wound Dehiscence; Trial of Labor
PubMed: 33030734
DOI: 10.5603/GP.2020.0086 -
Cureus May 2022Background and objective Corneal transplants are associated with multiple well-known complications, one of them being wound dehiscence. It results in unfavorable...
Background and objective Corneal transplants are associated with multiple well-known complications, one of them being wound dehiscence. It results in unfavorable outcomes, such as ocular structure damage, graft failure, repeated surgeries, and possibly a poor prognosis in terms of vision quality. Although the wound site may appear well-healed clinically, as the strength at the graft-host junction is weak, the site is susceptible to dehiscence. Wound dehiscence can be secondary to suture removal and/or trauma. In this study, we assessed the incidence of wound dehiscence secondary to trauma following corneal transplant and evaluated its occurrence in terms of patient age, type of corneal transplant performed, duration between corneal transplantation and wound dehiscence, mechanism of injury, and final outcome. Methods This was a case series that included all patients who suffered from wound dehiscence secondary to trauma post-corneal transplant between January 1, 2009, and December 31, 2019, at Hospital Kuala Lumpur. Results A total of 492 patients underwent corneal transplant surgery during the 10-year study period. Based on specified inclusion and exclusion criteria, only 13 patients were eligible for inclusion in this study. The incidence of wound dehiscence secondary to trauma post-penetrating keratoplasty (PK) was low (2.64%). Twelve patients had undergone PK, while one patient had undergone deep anterior lamellar keratoplasty (DALK). Blunt ocular trauma post-transplant can cause wound dehiscence regardless of patient age and duration post-corneal transplantation. Males are at a higher risk as their active lifestyle contributes to higher exposure to ocular injury. Conclusion As corneal transplant patients are at life-long risk of wound dehiscence post-transplant, they must be counseled about this possible risk and the need to take adequate precautions in their daily lives. Based on our findings, the use of newer technologies and partial- rather than full-thickness corneal transplants should be explored further.
PubMed: 35746998
DOI: 10.7759/cureus.25184 -
Journal of Ginseng Research Mar 2022Ginseng, officially known as Meyer, has been traditionally used as a medicinal herb, particularly in Asia. Ginseng is propagated from seeds; however, seed germination...
BACKGROUND
Ginseng, officially known as Meyer, has been traditionally used as a medicinal herb, particularly in Asia. Ginseng is propagated from seeds; however, seed germination is challenging, especially in its natural environment on farms. The seeds typically exhibit morphophysiological dormancy and require release from both morphological and physiological dormancy before germination. Although some studies have proposed methods for increasing seed germination rates, the underlying mechanisms of its dormancy release process remain unclear. Here, we investigated metabolic alterations during dehiscence in to determine their potential roles in dormancy release.
METHODS
We compared the ginseng seed metabolome before and after dehiscence and the ginsenoside and phytosterol compositions of the seeds in both periods in the presence of related enzymes.
RESULTS
After seed dehiscence, the sugar, amino acid, and squalene concentrations were significantly altered, phytosterols associated with the stigmasterol biosynthesis pathway were increased, while ginsenoside and brassinosteroid levels were not significantly altered. In addition, squalene epoxidase, cycloartenol synthase, 24-methylenesterol C-methyltransferase, and the stigmasterol biosynthesis pathway were activated.
CONCLUSION
Overall, our findings suggest that morphological activities that facilitate ginseng seed growth are the primary phenomena occurring during the dehiscence process. This study improves the understanding of germination processes and promotes further research of its germination and cultivation.
PubMed: 35509817
DOI: 10.1016/j.jgr.2021.06.005 -
Cureus Mar 2022Background No study has evaluated the diagnostic accuracy of cone-beam computed tomography (CBCT) imaging in detecting bone defects in orthodontic patients with Class I...
The Diagnostic Accuracy of Cone-Beam Computed Tomography (CBCT) Imaging in Detecting and Measuring Dehiscence and Fenestration in Patients With Class I Malocclusion: A Surgical-Exposure-Based Validation Study.
Background No study has evaluated the diagnostic accuracy of cone-beam computed tomography (CBCT) imaging in detecting bone defects in orthodontic patients with Class I malocclusions. This study aimed to evaluate the accuracy of CBCT in detecting dehiscences and fenestrations before orthodontic treatment compared to the gold standard i.e., the actual clinical detection of bone defects on surgical exposure. Methods A validation study was undertaken at the Department of Orthodontics, University of Damascus between 29 August 2018 and 1 November 2020. The sample included 16 patients who had Class I malocclusion with moderate crowding on the lower anterior teeth. Results The proportion of dehiscence diagnosed on CBCT images was approximately two-and-a-half times greater than that found on direct examination i.e., 42.7% versus 17.7%, respectively. The proportion of fenestrations was almost three times greater when diagnosed on CBCT images compared to the gold standard i.e., 39.5% versus 13.5%, respectively. The sensitivity of CBCT imaging in detecting dehiscence and fenestration was 100%. The specificity of CBCT imaging ranged from 45.5% to 86.7% and from 50% to 86.7% for dehiscence and fenestration detection, respectively. Also, the diagnostic accuracy of CBCT imaging ranged from 44% to 87.5% and from 56% to 87.5% for dehiscence and fenestration detection, respectively. Conclusions The proportion of dehiscence diagnosed on CBCT images was approximately two-and-a-half times greater than that found on direct examination, and the proportion of fenestrations was almost three times greater when diagnosed on CBCT images compared to the gold standard. The CBCT overestimates the dimensions of the linear measurements compared to those measured by the gold standard.
PubMed: 35291727
DOI: 10.7759/cureus.22789 -
Cureus May 2023Background In this study, we aimed to determine if there is a difference in the rates of wound dehiscence, delayed union, nonunion, and unanticipated surgery after the...
Background In this study, we aimed to determine if there is a difference in the rates of wound dehiscence, delayed union, nonunion, and unanticipated surgery after the use of bioabsorbable local antibiotic-delivery systems (LADS), specifically comparing antibiotic-impregnated calcium sulfate pellets (Osteoset-T, Wright Medical Technology Inc., Arlington, TN, USA, hereafter referred to as beads) and chitosan sponge (Sentrex BioSponge, Bionova Medical, Germantown, TN, USA, hereafter referred to as sponges) in the management of acute and chronic extremity wounds. Methodology We conducted a retrospective comparative cohort study in the setting of a level 1 trauma center. All patients who received either beads or sponges as an adjunct to surgical debridement from January 2010 to December 2017 were included, and 136 patients met the inclusion criteria. The intervention studied was extremity wounds that were treated with bioabsorbable LADS, either beads or sponges. The main outcome measurement was wound dehiscence and the need for unanticipated surgery. Results Of the 136 patients in the study cohort, 78% (106/136) were treated with beads, and 22% (30/136) were treated with sponges. Of the 136 patients, 50 (37%) experienced wound dehiscence, and 49 patients required unanticipated surgery. Overall, 62% (31/50) of patients with wound dehiscence and 67.4% (33/49) of patients requiring unanticipated surgery were seen in the bead cohort (p = 0.0001 and 0.025, respectively). However, in multivariable analyses, we found that the odds of having wound dehiscence and undergoing unanticipated surgery were, respectively, 4.9 (p = 0.001) and 2.8 (p = 0.021) times more likely to occur in the sponge than in the bead group. Conclusions Sentrex sponges appear to be associated with higher rates of wound dehiscence and the need for unanticipated surgery compared to Osteoset beads.
PubMed: 37273303
DOI: 10.7759/cureus.38490 -
American Journal of Audiology Sep 2021Purpose The purpose of this clinical focus article is to describe a new method for assessment of superior semicircular canal dehiscence by laying the patient supine...
Purpose The purpose of this clinical focus article is to describe a new method for assessment of superior semicircular canal dehiscence by laying the patient supine during Valsalva-induced nystagmus testing. Method The traditional Valsalva-induced nystagmus test is described, followed by a new method for assessment of superior semicircular dehiscence conducted by laying the patient supine during testing. A case study is presented to illustrate this new testing technique known as the Supine Superior Semicircular Canal Dehiscence Test. Results It is hypothesized that during Valsalva-induced nystagmus testing performed in the upright, seated position, the dura mater could potentially seal the superior semicircular canal fistula, thereby concealing a defect in the bony labyrinth and yielding a false-negative test. To circumvent this, the patient should be placed in the supine position during Valsalva-induced nystagmus testing in order to prevent the dura mater from inadvertently sealing itself against the petrous portion of the temporal bone. The Supine Superior Semicircular Canal Dehiscence Test may reveal the defect in the bony labyrinth and improve the sensitivity of the Valsalva-induced nystagmus test. Conclusions The Supine Superior Semicircular Canal Dehiscence Test may be more sensitive for identifying superior semicircular canal dehiscence in patients with traditional symptoms and a negative Valsalva-induced nystagmus test in the seated position. While a case study is presented to illustrate the potential benefits of including the Supine Superior Semicircular Canal Dehiscence Test in the battery of diagnostic tests, further research is needed in larger samples.
Topics: Humans; Nystagmus, Pathologic; Semicircular Canal Dehiscence; Semicircular Canals; Temporal Bone; Vestibular Diseases
PubMed: 34153201
DOI: 10.1044/2021_AJA-21-00011 -
Visceral Medicine Jun 2017Anastomotic leakages substantially influence the outcome of patients after major surgery of the upper gastrointestinal tract. Endoscopy is important for making a... (Review)
Review
Anastomotic leakages substantially influence the outcome of patients after major surgery of the upper gastrointestinal tract. Endoscopy is important for making a diagnosis and for managing anastomotic leakages. By means of endoscopic inspection of the anastomotic site, not only the size and position of dehiscences but also the blood supply of the anastomotic region and an imminent leakage can be assessed. To close anastomotic leakages, several therapeutic methods are available, i.e. stenting, clipping, and the application of glue. In the majority of cases, the endoscopic application of a sponge for permanent suction (endoscopic vacuum-assisted closure therapy) is promising.
PubMed: 28785568
DOI: 10.1159/000475783 -
Journal of Neurological Surgery. Part... Aug 2021Tegmen tympani dehiscence in temporal multidetector computed tomography (MDCT) and superior semicircular canal dehiscence may be seen together. We investigated...
Tegmen tympani dehiscence in temporal multidetector computed tomography (MDCT) and superior semicircular canal dehiscence may be seen together. We investigated superior semicircular canal dehiscence in temporal MDCT and temporal magnetic resonance imaging (MRI). In this retrospective study, 127 temporal MRI and MDCT scans of the same patients were reviewed. In all, 48.8% ( = 62) of cases were male, and 51.2% ( = 65) of cases were female. Superior semicircular canal dehiscence and superior semicircular canal-temporal lobe distance were evaluated by both MDCT and MRI. Tegmen tympani dehiscence was evaluated by MDCT. Superior semicircular canal dehiscence was detected in 14 cases (5.5%) by temporal MDCT and 15 cases (5.9%) by temporal MRI. In 13 cases (5.1%), it was detected by both MDCT and MRI. In one case (0.4%), it was detected by only temporal MDCT, and in two cases (0.8%), it was detected by only temporal MRI. Median superior semicircular canal-to-temporal distance was 0.66 mm in both males and females in temporal MDCT and temporal MRI. In both temporal MDCT and temporal MRI, as superior semicircular canal-to-temporal lobe distance increased, the presence of superior semicircular canal dehiscence in temporal MDCT and temporal MRI decreased. Tegmen tympani dehiscence was detected in eight cases (6.3%) on the right side and six cases (4.7%) on the left side. The presence of tegmen tympani dehiscence in temporal MDCT and the presence of superior semicircular dehiscence in MDCT and MRI increased. Superior semicircular canal dehiscence was detected by both MDCT and MRI. Due to the accuracy of the MRI method to detect superior semicircular dehiscence, we recommend using MRI instead of MDCT to diagnose superior semicircular canal dehiscence. Moreover, there is no radiation exposure from MRI.
PubMed: 35573917
DOI: 10.1055/s-0040-1714107 -
Journal of the American Veterinary... Jul 2021To evaluate short-term risk factors associated with dehiscence and death in cats undergoing full-thickness large intestinal incisions.
OBJECTIVE
To evaluate short-term risk factors associated with dehiscence and death in cats undergoing full-thickness large intestinal incisions.
ANIMALS
84 client-owned cats that had undergone full-thickness large intestinal incisions and for which information regarding outcome through postoperative day 7 was available.
PROCEDURES
Medical records from 4 veterinary teaching hospitals were reviewed. For cats that met the inclusion criteria, signalment, history, laboratory test results, surgical and medical procedures, perioperative complications, and outcome were analyzed. A Fisher exact or Wilcoxon rank sum test was used to identify individual variables associated with dehiscence of intestinal incisions or patient nonsurvival to hospital discharge or both.
RESULTS
84 cats met the inclusion criteria. The overall dehiscence and survival to hospital discharge rates were 8.3% (7/84 cats) and 94% (79/84 cats), respectively. Factors associated with dehiscence and nonsurvival to hospital discharge included presence of band neutrophils, performance of partial colectomy with colonic resection and anastomosis, administration of blood products, postoperative cardiopulmonary arrest, and incisional inflammation or infection. Factors associated with nonsurvival to hospital discharge only included low serum globulin concentration, repair of colonic trauma or dehiscence, and postoperative colonic dehiscence. Factors associated with dehiscence only included hypoalbuminemia, renal dysfunction, administration of blood products or > 2 classes of antimicrobials, and intra-abdominal fecal contamination.
CONCLUSIONS AND CLINICAL RELEVANCE
Results indicated that intestinal dehiscence and mortality rates associated with large intestinal incisions in cats may be higher than previously proposed, although the risk of either outcome was still low. Factors suggestive of systemic illness were associated with colonic dehiscence or death, and focused prospective studies of risk factors are warranted.
Topics: Anastomosis, Surgical; Animals; Cats; Intestine, Large; Prospective Studies; Retrospective Studies; Risk Factors
PubMed: 34227864
DOI: 10.2460/javma.259.2.162