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Periodontology 2000 Jun 2021Ecologists have long recognized the importance of spatial scale in understanding structure-function relationships among communities of organisms within their... (Review)
Review
Ecologists have long recognized the importance of spatial scale in understanding structure-function relationships among communities of organisms within their environment. Here, we review historical and contemporary studies of dental plaque community structure in the context of three distinct scales: the micro (1-10 µm), meso (10-100 µm) and macroscale (100 µm to ≥1 cm). Within this framework, we analyze the compositional nature of dental plaque at the macroscale, the molecular interactions of microbes at the microscale, and the emergent properties of dental plaque biofilms at the mesoscale. Throughout our analysis of dental plaque across spatial scales, we draw attention to disease and health-associated structure-function relationships and include a discussion of host immune involvement in the mesoscale structure of periodontal disease-associated biofilms. We end with a discussion of two filamentous organisms, Fusobacterium nucleatum and Corynebacterium matruchotii, and their relevant contributions in structuring dental plaque biofilms.
Topics: Biofilms; Corynebacterium; Dental Plaque; Fusobacterium nucleatum; Humans; Microbiota
PubMed: 33690940
DOI: 10.1111/prd.12364 -
Nutrients Jul 2023Dental caries is a common and multifactorial biofilm disease that is associated with dietary habits and microbiota. Among the various pathogens inducing caries, is the...
Dental caries is a common and multifactorial biofilm disease that is associated with dietary habits and microbiota. Among the various pathogens inducing caries, is the most extensively studied. Promoting oral health with probiotics has gained considerable attention. () strains were reported to modulate the gut microbiota and enhance host resistance to disease. Our previous research has found that ET-22 (ET-22) could inhibit biofilms in vitro. However, the preventive effect in vivo and functional mechanism of ET-22 on dental caries were unclear. In this study, the preventive effects of ET-22 on dental caries in mice were checked. Meanwhile, the functional mechanism of ET-22 was further investigated. Results showed that the supplementation of ET-22 in drinking water significantly improved the caries scoring of mice. The microbiota of dental plaques revealed that the live and heat-killed ET-22 similarly regulated the microbial structure in plaque biofilms. Functional prediction of PICRUSt showed that the addition of live and heat-killed ET-22 may inhibit biofilm formation. By the in vitro trials, the live and heat-killed ET-22 indeed inhibited the construction of biofilms and EPS productions of biofilms. This evidence suggests that ET-22 can restrain dental caries by regulating the microbiota of dental plaques and inhibiting biofilm formation, which may be partly mediated by the body components of ET-22.
Topics: Mice; Animals; Lacticaseibacillus paracasei; Dental Caries; Dental Plaque; Streptococcus mutans; Biofilms; Microbiota
PubMed: 37571254
DOI: 10.3390/nu15153316 -
BioMed Research International 2015Black stain is characterized as a dark line or an incomplete coalescence of dark dots localized on the cervical third of the tooth. Over the last century, the etiology... (Review)
Review
Black stain is characterized as a dark line or an incomplete coalescence of dark dots localized on the cervical third of the tooth. Over the last century, the etiology of black stain has been the subject of much debate. Most of the studies concerning this issue were conducted in pediatric population. According to the reviewed articles published between 2001 and 2014, the prevalence of black stain varies from 2.4% to 18% with equal sex distribution. The majority of the authors confirm the correlation between the presence of black stain and lower caries experience. The microflora of this deposit is dominated by Actinomyces spp. and has lower cariogenic potential than nondiscolored dental plaque. Iron/copper and sulfur complexes are thought to be responsible for the dark color. In patients with black stain saliva has higher calcium concentrations and higher buffering capacity. Factors such as dietary habits, socioeconomic status, and iron supplementation may be contributing to the formation of black stain.
Topics: Dental Caries; Dental Plaque; Humans; Prevalence; Saliva; Tooth Discoloration
PubMed: 25802850
DOI: 10.1155/2015/469392 -
PloS One 2022Detecting and monitoring dental plaque is an important issue in research and clinical practice. In this context, new digital imaging methods that permit permanent...
Detecting and monitoring dental plaque is an important issue in research and clinical practice. In this context, new digital imaging methods that permit permanent documentation of the clinical findings could be promising tools. The aim of the study was therefore to investigate whether disclosed plaque can be reliably visualised on 2D and 3D images captured with digital intraoral imaging devices. Clinical examination was the reference method. Twenty subjects (27.5±1.2 years) were included and plaque was measured at three different stages: habitual plaque (T1), after 72 h without oral hygiene (T2) and after a subsequent habitual brushing exercise (T3). At each time point, plaque was disclosed followed by the clinical examination and capturing the 2D and 3D images (intraoral-camera CS 1500 and intraoral-scanner CS 3600; Carestream Dental, Germany). Plaque amounts were recorded on oral and vestibular surfaces of the Ramfjord-teeth (16, 21, 24, 36, 41, 44) using the Rustogi-modified-Navy-Plaque-Index (RMNPI) and expressed as percentage of plaque-containing RMNPI areas of all RMNPI areas. At T1, percentages (mean±SD) obtained from the clinical examination, 2D and 3D images were 62.2±10.6, 65.1±10.0 and 64.4±10.6 resp. increasing to 76.9±8.0, 77.9±8.6 and 77.5±9.4 resp. at T2. After toothbrushing (T3), values decreased to 56.3±11.1, 58.2±12.1 and 61.2±10.8 resp. All methods were able to show statistically significant changes in plaque amounts at the different time points with in part statistically significant but minor differences between them. The Bland-Altmann analysis revealed a good agreement between values from both 2D and 3D images with the clinical examination. The agreement of the scores obtained with the both image-based methods for the single RMNPI areas with the clinical examination was mainly classified as substantial to almost perfect. Amounts of plaque can be reliably detected and monitored on 2D images from an intraoral camera and on 3D images from an intraoral scanner.
Topics: Adult; Dental Plaque; Female; Humans; Imaging, Three-Dimensional; Male; Photography, Dental; Prospective Studies
PubMed: 35167618
DOI: 10.1371/journal.pone.0263722 -
Virulence 2015As our knowledge of host-microbial interactions within the oral cavity increases, future treatments are likely to be more targeted. For example, efforts to target a... (Review)
Review
As our knowledge of host-microbial interactions within the oral cavity increases, future treatments are likely to be more targeted. For example, efforts to target a single species or key virulence factors that they produce, while maintaining the natural balance of the resident oral microbiota that acts to modulate the host immune response would be an advantage. Targeted approaches may be directed at the black-pigmented anaerobes, Porphyromonas gingivalis and Prevotella intermedia, associated with periodontitis. Such pigments provide an opportunity for targeted phototherapy with high-intensity monochromatic light. Functional inhibition approaches, including the use of enzyme inhibitors, are also being explored to control periodontitis. More general disruption of dental plaque through the use of enzymes and detergents, alone and in combination, shows much promise. The use of probiotics and prebiotics to improve gastrointestinal health has now led to an interest in using these approaches to control oral disease. More recently the potential of antimicrobial peptides and nanotechnology, through the application of nanoparticles with biocidal, anti-adhesive and delivery capabilities, has been explored. The aim of this review is to consider the current status as regards non-conventional treatment approaches for oral infections with particular emphasis on the plaque-related diseases.
Topics: Animals; Bacteria, Anaerobic; Dental Plaque; Detergents; Glycoside Hydrolases; Humans; Mouth; Nanoparticles; Periodontitis; Phototherapy; Porphyromonas gingivalis; Prebiotics; Prevotella intermedia; Probiotics; Protease Inhibitors
PubMed: 25668296
DOI: 10.4161/21505594.2014.983783 -
The Journal of International Medical... Oct 2017Objective The natural accumulation of supragingival plaque on surfaces of human teeth is associated with gingival inflammation and the initiation of common oral...
Objective The natural accumulation of supragingival plaque on surfaces of human teeth is associated with gingival inflammation and the initiation of common oral diseases. This study evaluated the distribution of dental plaque and gingivitis scores within the dental arches after prophylaxis. Methods Adult subjects from the Dharwad, India area representing the general population who provided written informed consent were scheduled for screening. Healthy subjects over the age of 18 years, not currently requiring any medical or dental care, and presenting with a complement of at least 20 natural teeth were recruited for this parallel design study. Enrolled subjects (n = 41) underwent oral examinations for dental plaque (PI) and gingivitis (GI) using the Turesky modification of the Quigley-Hein and the Löe-Silness Index, respectively, at the baseline visit, followed by a whole mouth dental prophylaxis. Subjects were given fluoride toothpaste for twice daily oral hygiene for the next 30 days. Subjects were recalled on days 15 and 30 for PI and GI examinations identical to baseline. Results Analyses indicated that mean scores for PI and GI on either arch and the whole mouth were higher than 2 and 1, respectively, during all examinations. Anterior surfaces consistently exhibited lower PI scores than posterior regions of either arch, or the entire dentition. Regional GI differences within the dentition were similar to PI scores, with lower scores on anterior than posterior teeth. Prophylaxis reduced both the frequency and mean scores of both PI and GI, irrespective of arch, with lower scores observed on anterior than posterior regions during all recall visits. Molar and lingual regions consistently exhibited higher PI and GI scores compared with anterior surfaces. At all examinations, mean scores for both plaque and gingivitis were higher on approximal vestibular than mid-vestibular surfaces. Conclusions Differences observed in PI and GI within the dentition have several practical implications: (a) there are advantages of whole mouth assessments for oral health (b) a need for oral hygiene formulations to reduce the larger deposits of dental plaque in the posterior region and resultant gingival inflammation, and
Topics: Adolescent; Adult; Dental Arch; Dental Plaque; Dentition; Female; Gingiva; Gingivitis; Humans; Male; Middle Aged; Young Adult
PubMed: 28795618
DOI: 10.1177/0300060517705476 -
Phytotherapy Research : PTR Feb 2018This is a systematic review of clinical and laboratory studies evaluating the effect of Melaleuca alternifolia on periodontopathogens, dental plaque, gingivitis,... (Review)
Review
This is a systematic review of clinical and laboratory studies evaluating the effect of Melaleuca alternifolia on periodontopathogens, dental plaque, gingivitis, periodontitis, and inflammatory responses. The PubMed, Cochrane, Web of science, Bireme, Lilacs, Prospero, Open Grey, and Clinical Trials databases were searched to identify potentially eligible studies through October 2016. Of 1,654 potentially eligible studies, 25 were included in the systematic review. Their methodology was evaluated through the Cochrane Handbook for clinical studies and the GRADE system for in vivo/in vitro studies. Although clinical studies must be interpreted with caution due to methodological limitations, laboratory studies have found promising results. In vitro evidences showed that M. alternifolia has bactericidal and bacteriostatic effects against the most prevalent periodontopathogens. Clinical studies found comparable effects to chlorhexidine 0.12% in reducing gingival inflammation, although the antiplaque effect was lower. M. alternifolia also showed antioxidant properties, which are beneficial to the host, allied to the reduction on immune-inflammatory responses to pathogens. This systematic review suggests that the M. alternifolia has potential anti-inflammatory and antimicrobial properties, which can be easily applied to the periodontal tissues. However, further clinical trials are needed to elucidate the clinical relevance of its application.
Topics: Anti-Bacterial Agents; Dental Plaque; Humans; Melaleuca; Periodontal Diseases
PubMed: 29235165
DOI: 10.1002/ptr.5974 -
Periodontology 2000 Feb 2019This paper focuses on plaque control and the management of gingivitis in adults and summarizes the evidence of commercially available dentifrices as gathered from... (Review)
Review
This paper focuses on plaque control and the management of gingivitis in adults and summarizes the evidence of commercially available dentifrices as gathered from existing systematic reviews. Three internet sources were used to search for appropriate papers (up to and including February 2017). The search strategy was designed to include any systematic review published on dentifrices that also included an evaluation of plaque and gingivitis scores. Characteristics of the individual reviews, such as methodological aspects, quantitative data and conclusions, were extracted. The potential risk of bias was estimated and the acquired evidence was graded. Independent screening of 205 unique reviews resulted in 10 published and eligible systematic reviews. One publication evaluated the mechanical contribution of dentifrice to plaque removal. Eight papers were identified that evaluated the efficacy of a proposed single active ingredients, of which two reviewed more than one potentially active ingredient. One study compared two active ingredients. This meta-review appraised the current state of evidence and found that toothbrushing with a standard fluoride dentifrice does not provide an added effect for the mechanical removal of dental plaque. Evidence suggests that compared with a standard dentifrice, those containing triclosan or stannous fluoride have benefits with respect to gingival health and control of dental plaque.
Topics: Adult; Analysis of Variance; Dental Plaque; Dentifrices; Double-Blind Method; Gingivitis; Humans; Periodontal Index
PubMed: 30892760
DOI: 10.1111/prd.12257 -
British Dental Journal Dec 2022Dental calculus is recognised as a secondary aetiological factor in periodontal disease, and being a prominent plaque retentive factor, it is routinely removed by the... (Review)
Review
Dental calculus is recognised as a secondary aetiological factor in periodontal disease, and being a prominent plaque retentive factor, it is routinely removed by the dental team to maintain oral health. Conversely, dental calculus can potentially be useful in forensic studies by supplying data that may be helpful in the identification of human remains and assist in determining the cause of death. During the last few decades, dental calculus has been increasingly recognised as an informative tool to understand ancient diet and health. As an archaeological deposit, it may contain non-dietary debris which permits the exploration of human behaviour and activities. While optical and scanning electron microscopy were the original analytical methods utilised to study microparticles entrapped within the calcified matrix, more recently, molecular approaches, including ancient DNA (aDNA) and protein analyses, have been applied. Oral bacteria, a major component of calculus, is the primary target of these aDNA studies. Such analyses can detect changes in the oral microbiota, including those that have reflected the shift from agriculture to industrialisation, as well as identifying markers for various systemic diseases.
Topics: Humans; Dental Calculus; Oral Health; Archaeology; Dental Plaque; Microbiota
PubMed: 36494546
DOI: 10.1038/s41415-022-5266-7 -
Microbiology Spectrum Oct 2022Rheumatoid arthritis (RA) is influenced by oral and gut bacteria; however, much less is known about the relationship between oral or gut viromes and RA. Here, we...
Rheumatoid arthritis (RA) is influenced by oral and gut bacteria; however, much less is known about the relationship between oral or gut viromes and RA. Here, we performed whole-oral- and whole-gut-virome analyses based on shotgun sequencing of 497 samples. A comparative analysis of the oral and gut viromes in healthy controls and untreated and treated RA patients was performed, and system interaction networks among viruses, bacteria, and RA-associated clinical indices were constructed to address the potential relationship between the virome and RA by principal-coordinate analysis, distance-based redundancy analysis, permutational multivariate analysis, Spearman correlation coefficient analysis, and random-forest model analysis. The results showed that the viromes could be profiled in dental plaque, saliva, and fecal samples, among which saliva had the highest within-sample diversity. Importantly, significantly different diversities and compositions of the oral (i.e., dental plaque and saliva) viromes were observed not only between RA patients and healthy controls but also between untreated and treated RA patients, yet there were relatively minor differences in the gut viromes. Furthermore, to understand how these viruses affected the bacteriome, a virus-bacterium interaction network was constructed from dental plaque, saliva, and fecal samples of RA patients. Additionally, some RA-associated oral taxa, including phage (vOTU70), Bacteroides vulgatus, Lactococcus lactis, Escherichia coli, and Neisseria elongata, were correlated with the RA-related clinical indices. Whole-virome analysis illustrated the potential role of the oral and gut viromes in affecting our body either directly or via bacteria, which characterized neglected and new candidates contributing to the development of RA. Our results demonstrated community variation among dental plaque, saliva, and fecal viromes. In oral and gut samples from untreated and treated RA patients, the perturbance of viral composition and the correlation network of microbes and RA-associated clinical indices might be involved in the pathogenicity of RA. The findings in this study expand the knowledge of the potential role of oral and gut viral communities in the development of RA and may contribute to research on correlations between viruses and other diseases.
Topics: Humans; Virome; Dental Plaque; Dysbiosis; Viruses; Bacteria; Arthritis, Rheumatoid
PubMed: 36040159
DOI: 10.1128/spectrum.00348-22