-
Molecular Biology and Evolution Dec 2022Host-associated microbiomes are essential for a multitude of biological processes. Placed at the contact zone between external and internal environments, the...
Host-associated microbiomes are essential for a multitude of biological processes. Placed at the contact zone between external and internal environments, the little-studied oral microbiome has important roles in host physiology and health. Here, we investigate the roles of host evolutionary relationships and ecology in shaping the oral microbiome in three closely related gorilla subspecies (mountain, Grauer's, and western lowland gorillas) using shotgun metagenomics of 46 museum-preserved dental calculus samples. We find that the oral microbiomes of mountain gorillas are functionally and taxonomically distinct from the other two subspecies, despite close evolutionary relationships and geographic proximity with Grauer's gorillas. Grauer's gorillas show intermediate bacterial taxonomic and functional, and dietary profiles. Altitudinal differences in gorilla subspecies ranges appear to explain these patterns, suggesting a close connection between dental calculus microbiomes and the environment, likely mediated through diet. This is further supported by the presence of gorilla subspecies-specific phyllosphere/rhizosphere taxa in the oral microbiome. Mountain gorillas show a high abundance of nitrate-reducing oral taxa, which may promote adaptation to a high-altitude lifestyle by modulating blood pressure. Our results suggest that ecology, rather than evolutionary relationships and geographic distribution, shape the oral microbiome in these closely related species.
Topics: Animals; Gorilla gorilla; Phylogeny; Dental Calculus; Hominidae; Microbiota
PubMed: 36472532
DOI: 10.1093/molbev/msac263 -
Journal of Periodontal Research Apr 2022This meta-analysis of observational studies (PROSPERO registration number CRD42021236054) sought to investigate strength and generalizability of the association of... (Meta-Analysis)
Meta-Analysis Review
BACKGROUND AND OBJECTIVES
This meta-analysis of observational studies (PROSPERO registration number CRD42021236054) sought to investigate strength and generalizability of the association of herpes simplex virus type 1 (HSV-1) in subjects with plaque-induced gingivitis and periodontitis, since the data from literature are contrasting.
MATERIAL AND METHODS
Case-control and cross-sectional studies, investigating HSV-1 in subgingival plaque/crevicular fluid and periodontal status, were searched through MEDLINE via PubMed, Scopus, Web of Science and Google Scholar. From each study the crude odds ratio (OR) with 95% confidence interval (95CI) was extracted, and the pooled OR was assessed for periodontitis, chronic and aggressive, and gingivitis. The meta-analytic method was chosen based on the level of heterogeneity. The generalizability of results, determined by the meta-analysis bias, was investigated through secondary analyses including sensitivity analyses for study quality, publication bias, and study inclusion, and subgroup analyses for quality of scientific journals that published the primary studies, world Region, subgingival plaque sampling method and study design.
RESULTS
Twelve studies were included (738 cases, 551 controls). The pooled ORs were 4.4 (95CI, 1.9-10.2) for any periodontitis; specifically, 2.8 (95CI, 1.0-8.3) for chronic periodontitis, 11.8 (95CI, 5.4-25.8) for aggressive periodontitis and 4.8 (95CI, 2.1-11.0) for gingivitis. These estimates were statistically significant, excluding for chronic periodontitis, resulting marginally significant (p = .05). Secondary analyses on any and aggressive periodontitis, and, partly, chronic periodontitis corroborated the results, while the material was insufficient for secondary analyses on gingivitis.
CONCLUSIONS
The results obtained indicated that HSV-1 is associated with periodontitis, while data about gingivitis are inconclusive. HSV-1 investigation in subgingival plaque could help assess periodontitis risk and severity and, if causal association were confirmed, could contribute to its control.
Topics: Aggressive Periodontitis; Cross-Sectional Studies; Dental Plaque; Gingivitis; Herpesvirus 1, Human; Humans; Observational Studies as Topic
PubMed: 34978079
DOI: 10.1111/jre.12968 -
Scientific Reports Mar 2017We evaluated the association between oral health and mortality. The study population comprised 76,188 subjects aged 16-89 years at recruitment. The mean follow-up time...
We evaluated the association between oral health and mortality. The study population comprised 76,188 subjects aged 16-89 years at recruitment. The mean follow-up time was 3.4 ± 2.4 years. Subjects with a personal medical history of cancer or cardiovascular disease and death by casualty were excluded from the analysis. A full-mouth clinical examination was performed in order to assess dental plaque, dental calculus and gingival inflammation. The number of teeth and functional masticatory units <5 were recorded. Causes of death were ascertained from death certificates. Mortality risk was evaluated using Cox regression model with propensity score calibrated for each oral exposure. All-cause mortality risk were raised with dental plaque, gingival inflammation, >10 missing teeth and functional masticatory units <5. All-cancer mortality was positively associated with dental plaque and gingival inflammation. Non-cardiovascular and non-cancer mortality were also positively associated with high dental plaque (HR = 3.30, [95% CI: 1.76-6.17]), high gingival inflammation (HR = 2.86, [95% CI: 1.71-4.79]), >10 missing teeth (HR = 2.31, [95% CI: 1.40-3.82]) and functional masticatory units <5 (HR = 2.40 [95% CI 1.55-3.73]). Moreover, when ≥3 oral diseases were cumulated in the model, the risk increased for all-cause mortality (HR = 3.39, [95% CI: 2.51-5.42]), all-cancer mortality (HR = 3.59, [95% CI: 1.23-10.05]) and non-cardiovascular and non-cancer mortality (HR = 4.71, [95% CI: 1.74-12.7]). The present study indicates a postive linear association between oral health and mortality.
Topics: Adolescent; Adult; Aged; Aged, 80 and over; Cardiovascular Diseases; Dental Plaque; Female; Gingivitis; Humans; Male; Middle Aged; Mouth Diseases; Neoplasms; Oral Health; Proportional Hazards Models
PubMed: 28294149
DOI: 10.1038/srep44604 -
BMC Oral Health Jun 2021Toothbrushing is an important yet neglected behaviour that affects the oral health of preschool children. Little is reported on parental supervision, an essential aspect...
BACKGROUND
Toothbrushing is an important yet neglected behaviour that affects the oral health of preschool children. Little is reported on parental supervision, an essential aspect of routine effective toothbrushing in this age group. The aim of this study was to evaluate pre-schoolers' toothbrushing behaviour including parental involvement and its association with their oral health.
METHODS
This was a cross-sectional study. A total of 92 preschool children (4-6 years) were invited to participate with their parents/guardians. Nine parameters of toothbrushing behaviour were assessed from parental responses (questionnaire) and observation of child and parents/guardians (video recording). Oral examination included recording plaque, gingival and dental caries indices. BORIS software was used to assess toothbrushing parameters and Smart PLS was used to perform association with a second-generation multivariate analysis to create models with and without confounding factors.
RESULTS
Girls were slightly more (53%) than boys (47%). Children aged 4 years were slightly more in number (38%), followed by 6-year-olds and 5-year-olds. Nearly, 90% parents had tertiary education and 46% had more than 2 children. Differences were recorded in the reported and observed behaviour. Thirty-five percent parents/guardians reported using pea-size toothpaste amount but only 28% were observed. Forty percent reported to brush for 30 s-1 min, however 51% were observed to brush for 1-2 min. Half the children were observed to use fluoridated toothpaste (F < 1000 ppm) under parental supervision (11%). The mean (SD) plaque score reduction after toothbrushing was 10.80 (2.46), mean pre-brushing plaque score was 90.3 (10.2), mean gingival index was 0.89 (0.65) and mean dental caries status (ICDAS) was 18.87 (12.39). Toothbrushing behaviour in terms of toothbrushing technique, duration, pattern and frequency, toothbrush type and grip type, toothpaste type and amount, post-brushing mouth rinsing and parental involvement contributed significantly to plaque score change (86%), dental caries status (73%), gingival index (66%) and pre-brushing plaque score (31%). The significant confounding variables had a small influence on oral health of preschool children.
CONCLUSIONS
Preschool children's toothbrushing behaviour was inadequate while their oral health was poor, with a significant association between the two parameters.
Topics: Child; Child, Preschool; Cross-Sectional Studies; Dental Caries; Dental Plaque; Female; Humans; Male; Oral Health; Toothbrushing
PubMed: 34078349
DOI: 10.1186/s12903-021-01643-8 -
Hua Xi Kou Qiang Yi Xue Za Zhi = Huaxi... Oct 2017Mechanical instrumentation preparation alone is insufficient to completely remove root canal infection, and chemical irrigation is essential to eliminate infected... (Review)
Review
Mechanical instrumentation preparation alone is insufficient to completely remove root canal infection, and chemical irrigation is essential to eliminate infected remnants. An ideal root canal irrigant should completely remove the smear layer, lubricate the root canal, efficiently kill bacteria, induce mild or no inflammatory response in the tissues, and avoid damaging the dentin structure. However, a commercial irrigant that meets all these requirements is currently lacking. QMix is a root canal irrigation mixture of ethylenediamine tetraacetic acid (EDTA), chlorhexidine (CHX) and surfactant. This mixture can remove the smear layer efficiently, and it possesses strong antibacterial effect and good biocompatibility with minimal cytotoxicity. Furthermore, the influence of QMix on the color and micro-hardness of dentin is low, and it can improve the wettability of root canal sealant without affecting its adhesive properties. This review compares the efficiencies of QMix with other irrigants (sodium hypochlorite, CHX, EDTA, SmearClear, and MTAD) in term of smear layer removal, dentine and root canal sealing, cell cytotoxicity, and bacterial growth inhibition.
Topics: Dental Pulp Cavity; Dentin; Edetic Acid; Humans; Microscopy, Electron, Scanning; Root Canal Irrigants; Root Canal Preparation; Root Canal Therapy; Smear Layer; Sodium Hypochlorite
PubMed: 29188654
DOI: 10.7518/hxkq.2017.05.019 -
Rapid Communications in Mass... Jun 2022Dental calculus (mineralised dental plaque) is composed primarily of hydroxyapatite. We hypothesise that the carbonate component of dental calculus will reflect the...
RATIONALE
Dental calculus (mineralised dental plaque) is composed primarily of hydroxyapatite. We hypothesise that the carbonate component of dental calculus will reflect the isotopic composition of ingested simple carbohydrates. Therefore, dental calculus carbonates may be an indicator for sugar consumption, and an alternative to bone carbonate in isotopic palaeodiet studies.
METHODS
We utilised Fourier transform infrared attenuated total reflectance analysis to characterise the composition and crystallisation of bone and dental calculus before isotope analysis of carbonate. Using a Sercon 20-22 mass spectrometer coupled with a Sercon GSL sample preparation system and an IsoPrime 100 dual inlet mass spectrometer plus Multiprep device to measure carbon, we tested the potential of dental calculus carbonate to identify C resources in diet through analysis of δ C values in paired bone, calculus and teeth mineral samples.
RESULTS
The modern population shows higher δ C values in all three tissue carbonates compared to both archaeological populations. Clear differences in dental calculus δ C values are observed between the modern and archaeological individuals suggesting potential for utilising dental calculus in isotope palaeodiet studies. The offset between dental calculus and either bone or enamel carbonate δ C values is large and consistent in direction, with no consistent offset between the δ C values for the three tissues per individual.
CONCLUSIONS
Our results support dental calculus carbonate as a new biomaterial to identify C sugar through isotope analysis. Greater carbon fractionation in the mouth is likely due to the complex formation of dental calculus as a mineralized biofilm, which results in consistently high δ C values compared to bone and enamel.
Topics: Carbon; Carbon Isotopes; Carbonates; Dental Calculus; Dietary Sugars; Humans; Isotopes; Sugars
PubMed: 35261104
DOI: 10.1002/rcm.9286 -
Frontiers in Cellular and Infection... 2014Dental caries remains a significant public health problem and is considered pandemic worldwide. The prediction of dental caries based on profiling of microbial species...
Dental caries remains a significant public health problem and is considered pandemic worldwide. The prediction of dental caries based on profiling of microbial species involved in disease and equally important, the identification of species conferring dental health has proven more difficult than anticipated due to high interpersonal and geographical variability of dental plaque microbiota. We have used RNA-Seq to perform global gene expression analysis of dental plaque microbiota derived from 19 twin pairs that were either concordant (caries-active or caries-free) or discordant for dental caries. The transcription profiling allowed us to define a functional core microbiota consisting of nearly 60 species. Similarities in gene expression patterns allowed a preliminary assessment of the relative contribution of human genetics, environmental factors and caries phenotype on the microbiota's transcriptome. Correlation analysis of transcription allowed the identification of numerous functional networks, suggesting that inter-personal environmental variables may co-select for groups of genera and species. Analysis of functional role categories allowed the identification of dominant functions expressed by dental plaque biofilm communities, that highlight the biochemical priorities of dental plaque microbes to metabolize diverse sugars and cope with the acid and oxidative stress resulting from sugar fermentation. The wealth of data generated by deep sequencing of expressed transcripts enables a greatly expanded perspective concerning the functional expression of dental plaque microbiota.
Topics: Anti-Bacterial Agents; Bacteria; Biofilms; Child; Child, Preschool; Cluster Analysis; Dental Caries; Dental Plaque; Drug Resistance, Bacterial; Gene Expression Profiling; Gene Expression Regulation; Gene Expression Regulation, Bacterial; Gene Regulatory Networks; Gene-Environment Interaction; Genetic Predisposition to Disease; Humans; Metagenome; Microbiota; Oxidative Stress; Phenotype; Transcription, Genetic
PubMed: 25177549
DOI: 10.3389/fcimb.2014.00108 -
Genomic ancestry, diet and microbiomes of Upper Palaeolithic hunter-gatherers from San Teodoro cave.Communications Biology Nov 2022Recent improvements in the analysis of ancient biomolecules from human remains and associated dental calculus have provided new insights into the prehistoric diet and...
Recent improvements in the analysis of ancient biomolecules from human remains and associated dental calculus have provided new insights into the prehistoric diet and genetic diversity of our species. Here we present a multi-omics study, integrating metagenomic and proteomic analyses of dental calculus, and human ancient DNA analysis of the petrous bones of two post-Last Glacial Maximum (LGM) individuals from San Teodoro cave (Italy), to reconstruct their lifestyle and the post-LGM resettlement of Europe. Our analyses show genetic homogeneity in Sicily during the Palaeolithic, representing a hitherto unknown Italian genetic lineage within the previously identified Villabruna cluster. We argue that this lineage took refuge in Italy during the LGM, followed by a subsequent spread to central-western Europe. Analysis of dental calculus showed a diet rich in animal proteins which is also reflected on the oral microbiome composition. Our results demonstrate the power of this approach in the study of prehistoric humans and will enable future research to reach a more holistic understanding of the population dynamics and ecology.
Topics: Humans; Animals; Proteomics; Dental Calculus; Diet; Genomics; Microbiota
PubMed: 36400919
DOI: 10.1038/s42003-022-04190-2 -
The Angle Orthodontist May 2022To evaluate the effect of nitrogen (N)-doped titanium dioxide (TiO2) coated stainless steel brackets activated with natural visible light and dental operating lights on...
OBJECTIVES
To evaluate the effect of nitrogen (N)-doped titanium dioxide (TiO2) coated stainless steel brackets activated with natural visible light and dental operating lights on Streptococcus mutans concentration in the plaque of orthodontic patients at 30 and 60 days.
MATERIALS AND METHODS
A total of 30 patients were recruited for this split-mouth study; 60 upper lateral incisor brackets constituted the study sample. A total of 30 brackets (15 right and 15 left) were coated with N-doped TiO2 using the (radio frequency) magnetron sputtering method. Plaque samples were collected at 30 days and 60 days after appliance placement. S mutans concentration was evaluated using real-time polymerase chain reaction.
RESULTS
At both time intervals, the concentration of S mutans in the control group was greater than that in the study group (P = .005). In both the study and the control groups, the S mutans concentrations significantly increased from 30 to 60 days (P = .005).
CONCLUSIONS
N-doped TiO2, on exposure to natural visible light and dental operating light, was effective in reducing the plaque concentration of S mutans in orthodontic patients. The efficacy was better at 30 days than at 60 days after placing the orthodontic appliances.
Topics: Dental Plaque; Humans; Nitrogen; Nitrogen Dioxide; Orthodontic Brackets; Stainless Steel; Streptococcus mutans; Surface Properties; Titanium
PubMed: 35072710
DOI: 10.2319/062521-510.1 -
The Bulletin of Tokyo Dental College Mar 2020Many types of interdental instrument, such as dental floss and interdental brushes, can be purchased easily and sell in large numbers. Many studies have compared the...
Many types of interdental instrument, such as dental floss and interdental brushes, can be purchased easily and sell in large numbers. Many studies have compared the effects of such instruments. Few studies have investigated their relationship with regions of residual plaque, however. The purpose of this study was to compare rates of plaque removal from the mesial and distal surfaces of the bilateral maxillary and mandibular premolars and molars among 3 types of interdental instrument: finger-winding-dental floss, holder-type dental floss, and an interdental brush. Prior to the experiment, the artificial teeth in a jaw model were removed for application of artificial plaque. The teeth were then replaced and the model attached to a phantom. An operator brushed the teeth in a posture close to that adopted in actual tooth brushing with each type of instrument, after which the plaque removal rate was compared among them. The rate of plaque removal using finger-winding floss was the highest, although this type of instrument is less easy to hold than the others. The rate with a handle-equipped interdental instrument showed a decrease in some regions, although it is easy to hold. The results of this study suggest that the type of interdental instrument used should differ depending on the region to be cleaned.
Topics: Dental Devices, Home Care; Dental Plaque; Dental Plaque Index; Humans; Molar; Toothbrushing
PubMed: 32074586
DOI: 10.2209/tdcpublication.2019-0005