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Scientific Reports Nov 2021Extreme conditions and the availability of determinate substrates in oil fields promote the growth of a specific microbiome. Sulfate-reducing bacteria (SRB) and...
Extreme conditions and the availability of determinate substrates in oil fields promote the growth of a specific microbiome. Sulfate-reducing bacteria (SRB) and acid-producing bacteria (APB) are usually found in these places and can harm important processes due to increases in corrosion rates, biofouling and reservoir biosouring. Biocides such as glutaraldehyde, dibromo-nitrilopropionamide (DBNPA), tetrakis (hydroxymethyl) phosphonium sulfate (THPS) and alkyl dimethyl benzyl ammonium chloride (ADBAC) are commonly used in oil fields to mitigate uncontrolled microbial growth. The aim of this work was to evaluate the differences among microbiome compositions and their resistance to standard biocides in four different Brazilian produced water samples, two from a Southeast Brazil offshore oil field and two from different Northeast Brazil onshore oil fields. Microbiome evaluations were carried out through 16S rRNA amplicon sequencing. To evaluate the biocidal resistance, the Minimum Inhibitory Concentration (MIC) of the standard biocides were analyzed using enriched consortia of SRB and APB from the produced water samples. The data showed important differences in terms of taxonomy but similar functional characterization, indicating the high diversity of the microbiomes. The APB and SRB consortia demonstrated varying resistance levels against the biocides. These results will help to customize biocidal treatments in oil fields.
Topics: Bacteria; Biodiversity; Biofilms; Biofouling; Corrosion; Culture Media; Desulfovibrio; Disinfectants; Environmental Microbiology; Geography; Glutaral; Microbial Sensitivity Tests; Microbiota; Oil and Gas Fields; RNA, Ribosomal, 16S; Steel; Sulfates; Water; Water Microbiology
PubMed: 34845279
DOI: 10.1038/s41598-021-02494-7 -
International Dental Journal Apr 2023The oral microbiological environment may be implicated in the corrosion of orthodontic metals. This study aimed to examine the prevalence of sulfate-reducing bacteria...
OBJECTIVES
The oral microbiological environment may be implicated in the corrosion of orthodontic metals. This study aimed to examine the prevalence of sulfate-reducing bacteria (SRB) in orthodontic patients undergoing fixed appliance treatment.
METHODS
Sixty-nine orthodontic and 69 healthy non-orthodontic participants were enrolled in the study. Supragingival and subgingivaloral biofilm were collected and tested for the presence of SRB. The DNA extraction, polymerase chain reaction (PCR), and 16sRNA Sanger sequencing method was performed from the SRB-positive samples. The sequenced PCR products were analysed and compared with databases to identify the bacterial genus.
RESULTS
Amongst 69 orthodontic patients, characteristic black precipitates developed in 14, indicating the presence of iron sulfides which demonstrates the likelihood of SRB. Alternatively, 2 out of 69 showed the presence of SRB in healthy non-orthodontic participants (controls). Desulfovibrio spp was confirmed by analyses of 16sRNA sequencing, which revealed that the SRB prevalence was 20% in the examined participants with orthodontic appliances.
CONCLUSIONS
The prevalence of SRB was found to be significantly higher amongst orthodontic patients compared to non-orthodontic participants. Presence of stainless steel in the oral environment may have facilitated the colonisation of SRB.
Topics: Humans; Desulfovibrio; Bacteria; Biofilms; Sulfates
PubMed: 36180285
DOI: 10.1016/j.identj.2022.07.007 -
Microbiome Dec 2018Currently, the effect of the bacterial community on cast iron corrosion process does not reach consensus. Moreover, some studies have produced contrasting results,...
BACKGROUND
Currently, the effect of the bacterial community on cast iron corrosion process does not reach consensus. Moreover, some studies have produced contrasting results, suggesting that bacteria can either accelerate or inhibit corrosion.
RESULTS
The long-term effects of the bacterial community on cast iron corrosion in reclaimed wastewater distribution systems were investigated from both spatial (yellow layer vs. black layer) and temporal (1-year dynamic process) dimensions of the iron coupon-reclaimed wastewater microcosm using high-throughput sequencing and flow cytometry approaches. Cast iron coupons in the NON and UV reactors suffered more severe corrosion than did those in the NaClO reactor. The bacterial community significantly promoted cast iron corrosion, which was quantified for the first time in the practical reclaimed wastewater and found to account for at least 30.5% ± 9.7% of the total weight loss. The partition of yellow and black layers of cast iron corrosion provided more accurate information on morphology and crystal structures for corrosion scales. The black layer was dense, and the particles looked fusiform, while the yellow layer was loose, and the particles were ellipse or spherical. Goethite was the predominant crystalline phase in black layers, while corrosion products mainly existed as an amorphous phase in yellow layers. The bacterial community compositions of black layers were distinctly separated from yellow layers regardless of disinfection methods. The NON and UV reactors had a more similar microbial composition and variation tendency for the same layer type than did the NaClO reactor. Biofilm development can be divided into the initial start-up stage, mid-term development stage, and terminal stable stage. In total, 12 potential functional genera were selected to establish a cycle model for Fe, N, and S metabolism. Desulfovibrio was considered to accelerate the transfer of Fe to Fe and speed up weight loss.
CONCLUSION
The long-term effect of disinfection processes on corrosion behaviors of cast iron in reclaimed wastewater distribution systems and the hidden mechanisms were deciphered for the first time. This study established a cycle model for Fe, N, and S metabolism that involved 12 functional genera and discovered the significant contribution of Desulfovibrio in promoting corrosion.
Topics: Bacteria; Biofilms; Bioreactors; Corrosion; DNA, Bacterial; Desulfovibrio; High-Throughput Nucleotide Sequencing; Iron; Iron Compounds; Minerals; Sequence Analysis, DNA; Spatio-Temporal Analysis; Wastewater
PubMed: 30545419
DOI: 10.1186/s40168-018-0610-5 -
MBio Jan 2021Formation of multispecies communities allows nearly every niche on earth to be colonized, and the exchange of molecular information among neighboring bacteria in such...
Formation of multispecies communities allows nearly every niche on earth to be colonized, and the exchange of molecular information among neighboring bacteria in such communities is key for bacterial success. To clarify the principles controlling interspecies interactions, we previously developed a coculture model with two anaerobic bacteria, (Gram positive) and Hildenborough (Gram negative, sulfate reducing). Under conditions of nutritional stress for , the existence of tight cell-cell interactions between the two bacteria induced emergent properties. Here, we show that the direct exchange of carbon metabolites produced by allows to duplicate its DNA and to be energetically viable even without its substrates. We identify the molecular basis of the physical interactions and how autoinducer-2 (AI-2) molecules control the interactions and metabolite exchanges between and (or and ). With nutrients, produces a small molecule that inhibits the AI-2 activity and could act as an antagonist Sensing of AI-2 by could induce formation of an intercellular structure that allows directly or indirectly metabolic exchange and energetic coupling between the two bacteria. Bacteria have usually been studied in single culture in rich media or under specific starvation conditions. However, in nature they coexist with other microorganisms and build an advanced society. The molecular bases of the interactions controlling this society are poorly understood. Use of a synthetic consortium and reducing complexity allow us to shed light on the bacterial communication at the molecular level. This study presents evidence that quorum-sensing molecule AI-2 allows physical and metabolic interactions in the synthetic consortium and provides new insights into the link between metabolism and bacterial communication.
Topics: Clostridium acetobutylicum; Coculture Techniques; Culture Media; DNA Replication; DNA, Bacterial; Desulfovibrio vulgaris; Energy Metabolism; Fluoresceins; Genes, Reporter; Homoserine; Lactones; Luminescent Proteins; Quorum Sensing; Signal Transduction; Red Fluorescent Protein
PubMed: 33468690
DOI: 10.1128/mBio.02758-20 -
Mediators of Inflammation 2020This study was conducted to analyze plasma reproductive hormone and biochemical parameter changes, as well as fecal microbiota composition and metabolites in sows, at...
This study was conducted to analyze plasma reproductive hormone and biochemical parameter changes, as well as fecal microbiota composition and metabolites in sows, at different pregnancy and lactation stages, using Bama mini pig as an experimental animal model. We found that plasma prolactin (PRL), progesterone, follicle-stimulating hormone (FSH), and estrogen levels decreased from day 45 to day 105 of pregnancy. Plasma total protein and albumin levels were lower in pregnant sows, while glucose, urea nitrogen, total cholesterol, and high-density lipoprotein-cholesterol, as well as fecal acetate, butyrate, valerate, total short-chain fatty acids, skatole, and tyramine levels, were higher in lactating sows. Interestingly, the lactating sows showed lower -diversity and and relative abundances, while pregnant sows showed a higher relative abundance. Notably, the relative abundance was highest on day 7 of lactation. Spearman analysis showed a positive correlation between plasma triglyceride and cholinesterase levels and and relative abundances. Moreover, and relative abundances were also positively correlated with plasma FSH, LH, and E levels, as well as PRL and LH with Collectively, plasma reproductive hormones, biochemical parameters, and fecal microbiota composition and metabolite levels could alter along with pregnancy and lactation, which might contribute to the growth and development demands of fetuses and newborns.
Topics: Akkermansia; Albumins; Animals; Bacteroides; Blood Proteins; Clostridiales; Desulfovibrio; Estrogens; Feces; Female; Follicle Stimulating Hormone; Lactation; Luteinizing Hormone; Microbiota; Pregnancy; Pregnancy, Animal; Progesterone; Prolactin; Proteobacteria; Spirochaetales; Streptococcus; Swine; Swine, Miniature; Verrucomicrobia
PubMed: 33162832
DOI: 10.1155/2020/8829072 -
Molecular Cell Sep 2016Bacteria employ surveillance complexes guided by CRISPR (clustered, regularly interspaced, short palindromic repeats) RNAs (crRNAs) to target foreign nucleic acids for...
Bacteria employ surveillance complexes guided by CRISPR (clustered, regularly interspaced, short palindromic repeats) RNAs (crRNAs) to target foreign nucleic acids for destruction. Although most type I and type III CRISPR systems require four or more distinct proteins to form multi-subunit surveillance complexes, the type I-C systems use just three proteins to achieve crRNA maturation and double-stranded DNA target recognition. We show that each protein plays multiple functional and structural roles: Cas5c cleaves pre-crRNAs and recruits Cas7 to position the RNA guide for DNA binding and unwinding by Cas8c. Cryoelectron microscopy reconstructions of free and DNA-bound forms of the Cascade/I-C surveillance complex reveal conformational changes that enable R-loop formation with distinct positioning of each DNA strand. This streamlined type I-C system explains how CRISPR pathways can evolve compact structures that retain full functionality as RNA-guided DNA capture platforms.
Topics: Amino Acid Motifs; Bacterial Proteins; Binding Sites; CRISPR-Cas Systems; Cloning, Molecular; Cryoelectron Microscopy; DNA; Desulfovibrio vulgaris; Endonucleases; Escherichia coli; Gene Editing; Gene Expression; Kinetics; Models, Molecular; Nucleic Acid Conformation; Operon; Protein Binding; Protein Conformation, alpha-Helical; Protein Interaction Domains and Motifs; RNA, Bacterial; RNA, Guide, CRISPR-Cas Systems; Recombinant Proteins; Substrate Specificity
PubMed: 27588603
DOI: 10.1016/j.molcel.2016.07.027 -
Scientific Reports Sep 2023Sulfate-reducing bacteria (SRB) are terminal members of any anaerobic food chain. For example, they critically influence the biogeochemical cycling of carbon, nitrogen,...
Sulfate-reducing bacteria (SRB) are terminal members of any anaerobic food chain. For example, they critically influence the biogeochemical cycling of carbon, nitrogen, sulfur, and metals (natural environment) as well as the corrosion of civil infrastructure (built environment). The United States alone spends nearly $4 billion to address the biocorrosion challenges of SRB. It is important to analyze the genetic mechanisms of these organisms under environmental stresses. The current study uses complementary methodologies, viz., transcriptome-wide marker gene panel mapping and gene clustering analysis to decipher the stress mechanisms in four SRB. Here, the accessible RNA-sequencing data from the public domains were mined to identify the key transcriptional signatures. Crucial transcriptional candidate genes of Desulfovibrio spp. were accomplished and validated the gene cluster prediction. In addition, the unique transcriptional signatures of Oleidesulfovibrio alaskensis (OA-G20) at graphene and copper interfaces were discussed using in-house RNA-sequencing data. Furthermore, the comparative genomic analysis revealed 12,821 genes with translation, among which 10,178 genes were in homolog families and 2643 genes were in singleton families were observed among the 4 genomes studied. The current study paves a path for developing predictive deep learning tools for interpretable and mechanistic learning analysis of the SRB gene regulation.
Topics: Humans; Transcriptome; Gene Expression Profiling; Desulfovibrio; Food Chain; Sulfates
PubMed: 37758719
DOI: 10.1038/s41598-023-43089-8 -
Ecotoxicology and Environmental Safety Dec 2020Smelting wastewater is characterized with high concentration of toxic heavy metals and high acidity, which must be properly treated before discharge. Here,... (Review)
Review
Smelting wastewater is characterized with high concentration of toxic heavy metals and high acidity, which must be properly treated before discharge. Here, bioelectrochemical system (BES) coupled with thermoelectric generator (TEG) was first demonstrated to simultaneously treat organic wastewater and smelting wastewater by utilizing the simulated waste heat that was abundant in smelting factories. By modulating the input voltage generated from simulated waste heat via TEG to 0, 1.0 and 2.0 V, almost all the Cu, Cd and Co in smelting wastewater were sequentially recovered with a respective rate of 121.17, 158.20 and 193.87 mg L d. Cu was bioelectrochemically recovered as Cu. While, Cd and Co were recovered by electrodeposition as Cd(OH), CdCO or Co(OH) on cathodic surface. High throughput sequencing analysis showed that the microbial community of anodic biofilm was greatly shifted after successive treatment by batch-mode. Desulfovibrio (17.00%), Megasphaera (11.81%), Geobacter (10.36%) and Propionibacterium (8.64%) were predominant genera in anodic biofilm enriched from activated sludge in BES before treatment. After successive treatment by batch-mode, Geobacter (34.76%), Microbacter (8.60%) and Desulfovibrio (5.33%) were shifted as the major genera. Economic analysis revealed that it was feasible to use TEG to substitute electrical grid energy to integrate with BES for wastewater treatment. In addition, literature review indicated that it was not uncommon for the coexistence of waste heat with typical pollutants (e.g. heavy metal ions and various biodegradation-resistant organic wastes) that could be treated by BES in different kinds of factories or geothermal sites. This study provides novel insights to expand the application potentials of BES by integrating with TEG to utilize widespread waste heat.
Topics: Bioelectric Energy Sources; Biofilms; Bioreactors; Electrochemical Techniques; Electrodes; Geobacter; Hot Temperature; Metals, Heavy; Sewage; Wastewater; Water Pollutants, Chemical; Water Purification
PubMed: 32853867
DOI: 10.1016/j.ecoenv.2020.111174 -
Nutrients Jan 2023Intestinal dysbiosis plays an important role in the pathogenesis of colitis (UC). can achieve anti-inflammatory effects as a medicine and food homologous vegetable....
Intestinal dysbiosis plays an important role in the pathogenesis of colitis (UC). can achieve anti-inflammatory effects as a medicine and food homologous vegetable. Luteolin, eriodictyol, fisetin, and kaempferol are the main anti-inflammatory active compounds obtained through mass spectrometry from the methanol extract of (JJSM). JJSM intervention resulted in attenuated weight loss, high disease-activity-index score, colon length shortening and colonic pathological damage in DSS-induced colitis mice. Interestingly, hydrogen sulfide (HS) was inhibited remarkably, which is helpful to elucidate the relationship between active substance and intestinal flora. Furthermore, JJSM administration improved intestinal flora with down-regulating the abundance of harmful bacteria such as and and up-regulating the abundance of beneficial bacteria such as and and enhanced the production of SCFAs. It is worth noticing that is related to the production of intestinal gas HS. The elevated levels of and HS will hasten the onset of colitis, which is a crucial risk factor for colitis. The results displayed that JJSM could considerably ameliorate colitis by rebuilding HS-related intestinal flora, which provides a new therapeutic strategy for to be utilized as a functional food and considered as an emerging candidate for intestinal inflammation.
Topics: Animals; Mice; Gastrointestinal Microbiome; Methanol; Colitis; Colon; Anti-Inflammatory Agents; Colitis, Ulcerative; Dextran Sulfate; Disease Models, Animal; Mice, Inbred C57BL
PubMed: 36771228
DOI: 10.3390/nu15030519 -
Journal of Advanced Research Jan 2021Increased numbers of sulfate-reducing bacteria (SRB) are often found in the feces of people and animals with inflammatory bowel disease. The final products of their... (Review)
Review
INTRODUCTION
Increased numbers of sulfate-reducing bacteria (SRB) are often found in the feces of people and animals with inflammatory bowel disease. The final products of their metabolism are hydrogen sulfide and acetate, which are produced during dissimilatory sulfate reduction process.
OBJECTIVES
The aim of the study was to monitor processes concerning sulfate reduction microbial metabolisms, including: the main microbial genera monitoring and their hydrogen sulfide production in the intestines of healthy and not healthy individuals, phylogenetic analysis of SRB isolates, cluster analysis of SRB physiological and biochemical parameters, SRB growth kinetic parameters calculation, same as the application of the two-factor dispersion analysis for finding relationship between SRB biomass accumulation, temperature and pH. Feces samples from healthy people and patients with colitis were used for isolation of sulfate-reducing microbial communities.
METHODS
Microbiological, biochemical, biophysical, molecular biology methods, and statistical processing of the results have been used for making an evaluation of gained results.
RESULTS
Two dominant SRB morphotypes differed in colony size and quantitative ratio in the feces of healthy and colitis patients were observed and identified. In the feces of healthy people, 93% of SRB of morphotype I prevailed while morphotype II made only 7% ; in the feces of patients with colitis, the ratio of these morphotypes was 99:1, respectively. Hydrogen sulfide concentrations are also higher in the feces of people with colitis and certain synergy effects exist among acetate produced by SRB.
CONCLUSIONS
The study results brought important findings concerning colony environments with developed colitis and these findings can lead to the development of possible risk indicators of ulcerative colitis prevalence.
PubMed: 33318867
DOI: 10.1016/j.jare.2020.03.007