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Medicine Dec 2023Lactational mastitis is a common and frequently disease in clinical practice, characterized by acute inflammation of the mammary ducts and surrounding connective...
BACKGROUND
Lactational mastitis is a common and frequently disease in clinical practice, characterized by acute inflammation of the mammary ducts and surrounding connective tissues. The main manifestations are damage to the mammary gland acini, edema, and invasion of inflammatory cells. If not treated properly, it may lead to the formation of breast abscesses, or even sepsis, septic shock, and chronic inflammation of the breast, which may cause the disease to persist or recur multiple times, so that the patients suffer extreme pain, and the health of both the mother and child are directly affected. This disease not only causes suffering for women but also may result in the cessation of breastfeeding. Therefore, rapid and effective treatment is particularly important.
CASE SUMMARY
We report 3 cases of lactation mastitis patients showing good clinical efficacy after being treated with the Chinese medicine Gualou Xiaoyong soup and painless lactation promoting techniques. Gualou Xiaoyong soup combined with painless lactation promotion techniques can significantly reduce and eliminate the clinical symptoms of patients in the short term, and rapidly restore inflammatory indicators such as total white blood cells, neutrophils, C-reactive protein, and procalcitonin to normal levels. The patchy low echo area of the breast under B-ultrasound also disappears quickly. Therefore, we believe that this method is a good way to treat lactational mastitis and is worthy of clinical reference and research. However, this study has certain limitations: this study lacks a large sample of prospective controlled studies. Next, we will continue to collect relevant cases and conduct prospective case randomized controlled clinical studies.
CONCLUSION
The treatment of lactation mastitis with Gualou Xiaoyong soup and painless lactation promoting techniques can achieve good clinical results.
Topics: Child; Female; Humans; Breast Feeding; Mastitis; Lactation; Breast; Empyema, Pleural
PubMed: 38050191
DOI: 10.1097/MD.0000000000036384 -
Veterinary Medicine and Science Sep 2023Mastitis and associated antimicrobial resistance (AMR) are major challenges to the dairy industry worldwide.
BACKGROUND
Mastitis and associated antimicrobial resistance (AMR) are major challenges to the dairy industry worldwide.
OBJECTIVE
This study aimed to expose the mastitis burden, causative bacteria and drivers for mastitis-causing multi-drug-resistant (MDR) Staphylococci infectivity in cows on dairy farms in Wakiso district, Uganda.
METHODS
On 22 farms, practices were documented using questionnaires, and 175 cows were screened by the California mastitis test. Composite milk samples from the positive reactors were submitted to the laboratory for bacterial culture testing. Antimicrobial sensitivity testing by the Kirby Bauer disc diffusion method was done only on Staphylococci with a panel of 10 antimicrobials of clinical relevance.
RESULTS
Mastitis was detected in 80.6% (n = 141) of the 175 sampled cows, of which sub-clinical mastitis (76.0%: n = 133) was predominant. The Chi-squared analysis hypothesized that cow age (p = 0.017), sub-county (p = 0.013), parity (p < 0.0001), sex of farm owner (p = 0.003), farm duration in dairy production (p = 0.048) and the use of milking salve (p = 0.006) were associated with mastitis. Coagulase-negative Staphylococci were the most prevalent (71.4%; n = 95), followed by Staphylococcus aureus (30.1%, n = 40). Staphylococci (76.3%; n = 135) were majorly resistant to penicillin and tetracycline. Only one isolate was phenotyped as a methicillin-resistant Staphylococcus specie (MRSS). The prevalences of MDR strains at cow and isolate level were 6.3% and 8.3%. The major MDR phenotype identified was penicillin-tetracycline-trimethoprim-sulphamethoxazole. The isolate detected as an MRSS exhibited the broadest MDR pattern. Cow parity was identified as a predictor of infectivity of mastitis-causing MDR Staphylococci in dairy herds.
CONCLUSION
The high prevalence of mastitis and associated pathogen AMR found exposes possibilities of economic losses for the dairy sector warranting the need for farmer sensitization on the institution of proper mastitis prevention and control programs, with emphasis on milking hygiene practices and routine disease monitoring.
Topics: Female; Pregnancy; Animals; Cattle; Farms; Uganda; Methicillin-Resistant Staphylococcus aureus; Bacteria; Mastitis; Anti-Bacterial Agents; Staphylococcus; Tetracycline; Penicillins; Cattle Diseases
PubMed: 37592441
DOI: 10.1002/vms3.1234 -
MCN. the American Journal of Maternal... 2017Biological and environmental changes to maternal and newborn microbiomes in the postnatal period can affect health outcomes for the mother-baby dyad. Postpartum sleep...
Biological and environmental changes to maternal and newborn microbiomes in the postnatal period can affect health outcomes for the mother-baby dyad. Postpartum sleep deprivation and unmet dietary needs can alter commensal bacteria within the body and disrupt gut-brain communication. Perineal injury and breast infections also change microbial community composition, potentiating an environment favoring pathogen growth. The gut microbiome refers to the collection of microorganisms working in harmony. Disruptions within the gut microbiome and gut-brain communication may lead to postpartum depression, a potentially devastating sequela. Postnatal newborn changes to the gut and skin microbiome materialize quickly after birth and are profoundly influenced by mode of birth, feeding method, and bathing and skin care practices. During the newborn period, infant microbiomes are highly vulnerable and susceptible to multiple influences. Maternal-newborn nurses have a valuable role in helping mothers and newborns promote healthy microbiomes. Factors that influence the rapidly changing postnatal microbiome of the mother and her newborn, and the role nurses have to positively influence immediate and long-term health outcomes are presented.
Topics: Adolescent; Adult; Feeding Behavior; Female; Gastrointestinal Microbiome; Humans; Infant, Newborn; Mastitis; Maternal-Fetal Exchange; Mothers; Postpartum Period; Pregnancy
PubMed: 29049057
DOI: 10.1097/NMC.0000000000000374 -
Annals of the Royal College of Surgeons... Mar 2023Idiopathic granulomatous mastitis (IGM) is an evolving problem with varied presentation. No definite treatment guidelines are available at present that may reduce rate...
INTRODUCTION
Idiopathic granulomatous mastitis (IGM) is an evolving problem with varied presentation. No definite treatment guidelines are available at present that may reduce rate of recurrence. Current evidence suggests a ductal pathology behind IGM, which leads to periductal mastitis, leakage and sinus/fistula formation. Thus, excision of the sinus/fistulous tract with en-bloc wide local excision (WLE) of the lesion could be curative. The objective of this study was to look for the basic aetiology of IGM and evaluate the effectiveness of WLE with total or partial duct excision as a curative approach.
METHODS
An institutional prospective comparative study was conducted over 4 years (2015-2019), in which 59 cases of IGM were randomly divided into three groups. After necessary investigations, patients in group A received steroid therapy, those in group B received WLE and patients in group C received WLE with total or partial duct excision as the mode of treatment. Postoperative follow-up was between 6 months and 3 years.
RESULTS
Histopathological examination (HPE) was found to be the most suitable diagnostic procedure. Patients in group B showed the highest rate of recurrence (73.6%), followed by group A (35.0%) and group C (5.0%). Patients in group C had a significantly lower chance of recurrence compared with both group A and group B (< 0.05). HPE reports of excised ducts from patients in group C showed ductal disruption and leakage along with periductal granuloma in 70% of cases.
CONCLUSIONS
The presence of duct granuloma indicates the association of ductal pathology in IGM. IGM is therefore a disease of the mammary ducts and en-bloc duct excision is curative in non-responding cases.
Topics: Female; Humans; Granulomatous Mastitis; Prospective Studies; Granuloma; Immunoglobulin M
PubMed: 35638904
DOI: 10.1308/rcsann.2022.0017 -
Journal of Dairy Science Oct 2021Current sensor systems are used to detect cows with clinical mastitis. Although, the systems perform well enough to not negatively affect the adoption of automatic...
Current sensor systems are used to detect cows with clinical mastitis. Although, the systems perform well enough to not negatively affect the adoption of automatic milking systems, the performance is far from perfect. An important advantage of sensor systems is the availability of multiple measurements per day. By clearly defining the need for detection of subclinical mastitis (SCM) and clinical mastitis (CM) from the farmers' management perspective, detection and management of SCM and CM may be improved. Sensor systems may also be used for other aspects of mastitis management. In this paper we have defined 4 mastitis situations that could be managed with the support of sensor systems. Because of differences in the associated management and the epidemiology of these specific mastitis situations, the required demands for performance of the sensor systems do differ. The 4 defined mastitis situations with the requirements of performance are the following: (1) Cows with severe CM needing immediate attention. Sensor systems should have a very high sensitivity (>95% and preferably close to 100%) and specificity (>99%) within a narrow time window (maximum 12 h) to ensure that close to all cows with true cases of severe CM are detected quickly. Although never studied, it is expected that because of the effects of severe CM, such a high detection performance is feasible. (2) Cows with mastitis that do not need immediate attention. Although these cows have a risk of progressing into severe CM or chronic mastitis, they should get the chance to cure spontaneously under close monitoring. Sensor alerts should have a reasonable sensitivity (>80%) and a high specificity (>99.5%). The time window may be around 7 d. (3) Cows needing attention at drying off. For selective dry cow treatment, the absence or presence of an intramammary infection at dry-off needs to be known. To avoid both false-positive and false-negative alerts, sensitivity and specificity can be equally high (>95%). (4) Herd-level udder health. By combining sensor readings from all cows in the herd, novel herd-level key performance indicators can be developed to monitor udder health status and development over time and raise alerts at significant deviances from predefined thresholds; sensitivity should be reasonably high, >80%, and because of the costs for further analysis of false-positive alerts, the specificity should be >99%. The development and validation of sensor-based algorithms specifically for these 4 mastitis situations will encourage situation-specific farmer interventions and operational udder health management.
Topics: Animals; Cattle; Cattle Diseases; Cell Count; Dairying; Escherichia coli Infections; Female; Mammary Glands, Animal; Mastitis; Mastitis, Bovine; Milk
PubMed: 34304877
DOI: 10.3168/jds.2020-19097 -
Journal of Dairy Science Apr 2020In dairy cattle, mastitis is a disease of the mammary gland caused by pathogens such as bacteria, viruses, fungi, and algae. Mastitis causes economic losses to dairy...
In dairy cattle, mastitis is a disease of the mammary gland caused by pathogens such as bacteria, viruses, fungi, and algae. Mastitis causes economic losses to dairy farms as well as public health concerns. The reproductive efficiency of commercial dairy herds has important implications for the economic success of dairy operations and is strongly associated with the health status of cows. Mastitis has previously been linked with decreased fertility of dairy cows, but the effect of specific pathogens on the severity of fertility reduction is still unclear. In this study, cows diagnosed with mastitis caused by major pathogens (Staphylococcus aureus, Streptococcus agalactiae, Escherichia coli, Klebsiella spp., Mycoplasma spp., and environmental Streptococcus) needed more artificial inseminations (AI) than did cows with mastitis caused by minor pathogens (coagulase-negative Staphylococcus and Corynebacterium spp.) and healthy cows. Cows diagnosed with mastitis, independent of what pathogen was causing mastitis, had more days open compared with nonmastitic cows. The percentage of cows that successfully established pregnancy at first AI was greater for the control group than for the major pathogens group but not significantly different from the minor pathogens group. Pregnancy loss was lower in the control group than in the major pathogens group; however, there was no difference compared with the minor pathogen group. Mastitis caused by gram-negative bacteria decreased the percentage of pregnancy per first AI and increased days open and pregnancy loss compared with the control group. Cows with mastitis caused by gram-positive bacteria also had increased days open compared with control cows. This study shows that different mastitis-causing bacteria can affect the fertility of cows differently. Mastitis events caused by major pathogens and gram-negative bacteria were associated with the greatest decrease in reproductive efficiency.
Topics: Animals; Bacteria; Bacterial Physiological Phenomena; Cattle; Female; Host Microbial Interactions; Insemination, Artificial; Mastitis; Mastitis, Bovine; Milk; Reproduction
PubMed: 32089296
DOI: 10.3168/jds.2019-16841 -
Journal of Veterinary Science May 2022The poor intracellular concentration of enrofloxacin might lead to treatment failure of cow mastitis caused by small colony variants (SASCVs).
BACKGROUND
The poor intracellular concentration of enrofloxacin might lead to treatment failure of cow mastitis caused by small colony variants (SASCVs).
OBJECTIVES
In this study, enrofloxacin composite nanogels were developed to increase the intracellular therapeutic drug concentrations and enhance the efficacy of enrofloxacin against cow mastitis caused by intracellular SASCVs.
METHODS
Enrofloxacin composite nanogels were formulated by an electrostatic interaction between gelatin (positive charge) and sodium alginate (SA; negative charge) with the help of CaCl (ionic crosslinkers) and optimized by a single factor test using the particle diameter, zeta potential (ZP), polydispersity index (PDI), loading capacity (LC), and encapsulation efficiency (EE) as indexes. The formation mechanism, structural characteristics, bioadhesion ability, cellular uptake, and the antibacterial activity of the enrofloxacin composite nanogels against intracellular SASCVs strain were studied systematically.
RESULTS
The optimized formulation was comprised of 10 mg/mL (gelatin), 5 mg/mL (SA), and 0.25 mg/mL (CaCl). The size, LC, EE, PDI, and ZP of the optimized enrofloxacin composite nanogels were 323.2 ± 4.3 nm, 15.4% ± 0.2%, 69.6% ± 1.3%, 0.11 ± 0.02, and -34.4 ± 0.8 mV, respectively. Transmission electron microscopy showed that the enrofloxacin composite nanogels were spherical with a smooth surface and good particle size distributions. In addition, the enrofloxacin composite nanogels could enhance the bioadhesion capacity of enrofloxacin for the SASCVs strain by adhesive studies. The minimum inhibitory concentration, minimum bactericidal concentration, minimum biofilm inhibitory concentration, and minimum biofilm eradication concentration were 2, 4, 4, and 8 μg/mL, respectively. The killing rate curve had a concentration-dependent bactericidal effect as increasing drug concentrations induced swifter and more radical killing effects.
CONCLUSIONS
This study provides a good tendency for developing enrofloxacin composite nanogels for treating cow mastitis caused by intracellular SASCVs and other intracellular bacterial infections.
Topics: Alginates; Animals; Anti-Bacterial Agents; Calcium Chloride; Cattle; Cattle Diseases; Enrofloxacin; Female; Gelatin; Mastitis; Nanogels; Staphylococcus aureus
PubMed: 35618320
DOI: 10.4142/jvs.21292 -
Cell Stress & Chaperones Nov 2023Mastitis is a disease involved in inflammation of breast which affects human and animals. Wogonin is one bioactive compound from many Chinese herbal medicines, which...
Mastitis is a disease involved in inflammation of breast which affects human and animals. Wogonin is one bioactive compound from many Chinese herbal medicines, which have multiple properties, including anti-inflammatory activity. However, the roles of wogonin in mastitis progression are largely undefined. Mastitis models were established using LPS-treated mice and mammary epithelial cells (MECs). Infiltration of inflammatory cells was analyzed by hematoxylin-eosin staining and myeloperoxidase (MPO) activity. Inflammatory cytokine (TNF-α and IL-1β) levels were detected via ELISA. The phosphorylation and total of Akt and NF-κB levels and content of Nrf2 and HO-1 were measured via western blot. Cell viability was examined by CCK-8 assay. Oxidative stress was assessed by ROS generation and levels of MDA, GSH, and SOD. Wogonin attenuated LPS-induced infiltration of inflammatory cells, increase of MPO activity and levels of TNF-α and IL-1β, and activation of the Akt/NF-κB pathway in murine mammary gland tissues, and promoted activation of Nrf2/HO-1 signaling. Wogonin did not affect MEC viability, but mitigated LPS-induced inflammation in MECs by reducing TNF-α and IL-1β levels. Wogonin relieved LPS-induced oxidative stress in MECs through decreasing ROS generation and MDA level and increasing GSH and SOD levels. Wogonin repressed LPS-induced activation of the Akt/NF-κB pathway in MECs and increased Nrf2/HO-1 signaling activation. Activated Akt/NF-κB signaling or Nrf2/HO-1 signaling inactivation reversed the suppressive effects of wogonin on LPS-induced inflammation and oxidative stress in MECs. Wogonin mitigates LPS-induced inflammation and oxidative stress of MECs via suppressing activation of the Akt/NF-κB signaling and activating Nrf2/HO-1 pathway, indicating the therapeutic potential of wogonin in mastitis.
Topics: Female; Humans; Animals; Mice; NF-kappa B; Lipopolysaccharides; Proto-Oncogene Proteins c-akt; NF-E2-Related Factor 2; Tumor Necrosis Factor-alpha; Reactive Oxygen Species; Inflammation; Mastitis; Oxidative Stress; Superoxide Dismutase
PubMed: 37910344
DOI: 10.1007/s12192-023-01391-4 -
Journal of Medical Case Reports Apr 2022Granulomatous mastitis is a rare inflammatory disease of the breast, typically seen in woman of child-bearing age. No definitive etiology has been described. In rare...
BACKGROUND
Granulomatous mastitis is a rare inflammatory disease of the breast, typically seen in woman of child-bearing age. No definitive etiology has been described. In rare instances, this condition has been reported to be associated with extramammary manifestations such as erythema nodosum and arthritis. We describe this rare condition in an adolescent female.
CASE PRESENTATION
A 16-year-old, Hispanic female presented with right-sided painful breast swelling, polyarthritis, and erythema nodosum on bilateral shins and lower thighs. Physical examination was negative for lymphadenopathy and pulmonary, gastrointestinal, and cardiovascular findings. Ophthalmologic examination for uveitis and serologic tests for autoimmune diseases were negative. Diagnosis of idiopathic granulomatous mastitis was made by exclusion of other etiologies and conditions. Confirmation was made by histopathologic examination demonstrating noncaseating granuloma within breast lobules with neutrophils and microabscess formation. After wide local excision and a short course of trimethoprim-sulfamethoxazole, our patient was placed on naproxen and prednisone, the latter being tapered off over 3 months, with steady and complete resolution of all symptoms.
CONCLUSION
This is the first reported case of idiopathic granulomatous mastitis in a pediatric patient who also had extramammary manifestations, including erythema nodosum and polyarthritis. In this case-based review, we summarize the phenotype, risk factors, prognosis, and treatment options of this rare condition, chiefly to make the readers cognizant of such a diagnostic possibility in similar clinical presentation in the future.
Topics: Adolescent; Arthritis; Child; Erythema Nodosum; Female; Granuloma; Granulomatous Mastitis; Humans; Prednisone
PubMed: 35382864
DOI: 10.1186/s13256-022-03327-5 -
Scientific Reports Oct 2019Little is known regarding the association of non-lactational mastitis women with breast cancer risk. This population-based cohort study examined the breast cancer risk...
Little is known regarding the association of non-lactational mastitis women with breast cancer risk. This population-based cohort study examined the breast cancer risk in women with non-lactational mastitis. We identified 3,091 women with non-lactational mastitis between 2000 and 2011 using the Taiwan National Health Insurance Research Database. We performed 1:4 propensity score matching by age, socioeconomic status and comorbidities and identified 12,364 women without non-lactational mastitis. The mean age of women with non-lactational mastitis was 37.9 years; these women had a higher breast cancer risk than the comparison group (adjusted hazard ratio = 1.94, 95% confidence interval: 1.30-2.90). The incidence rates of breast cancer in women with non-lactational mastitis and the comparison group were 14.79 and 7.57 per 10,000 person-years, respectively. Furthermore, non-lactational mastitis was a risk factor for breast cancer in women aged <50 years, women with lower socioeconomic status and women with hormonal medication (p < 0.05). Women who had more episodes of non-lactational mastitis had a higher risk of developing a breast cancer. Thus, the risk of breast cancer in women with non-lactational mastitis is significantly higher than those without non-lactational mastitis.
Topics: Adult; Breast Neoplasms; Cohort Studies; Female; Humans; Mastitis; Middle Aged; Risk
PubMed: 31666573
DOI: 10.1038/s41598-019-52046-3